FastQCFastQC Report
Thu 26 May 2016
SRR936085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6939093
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.1082274009009535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123850.034.6421621
CGTATCA20900.034.318527100-101
GTATCAA223800.028.6100061
CCTGTCG21500.028.37365796-97
CGGTATC20300.026.5363100-101
CCGTACA13450.026.33192100-101
TTATACG20100.026.20457696-97
CGTCTAG7350.024.700195100-101
ATCAACG267400.023.4961283
TCAACGC270750.023.2268814
CCGTATA8950.022.944778100-101
GACCGTA4700.022.79562698-99
CAACGCA276050.022.7584045
TATCAAC278650.022.6982842
TCGTATA9700.022.397985100-101
CCGTATC11650.021.970015100-101
TCGAGTA9800.021.562054100-101
CGAGCCG11600.021.54870896-97
CGATCTA4300.021.456116100-101
TACGCAG14600.021.403967100-101
Produced by FastQC (version 0.11.5)