FastQCFastQC Report
Thu 26 May 2016
SRR936084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961622
Sequences flagged as poor quality0
Sequence length77
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG24300.015.04579217
CGCACGA6250.014.1976354
GCCTCGA21750.013.38398621
CGAACTC21800.013.18835925
TCGAACT22350.012.54763124
ACGCTCG6800.012.53020645
CTCGAAC22750.012.32719123
GGCGGAA30800.012.10234420
CGGGGTG20650.012.03561249
GACGCGA17450.012.00949469
CGTGCGC11600.011.93670146
ACGCACG7500.011.8311073
GTAGACC41850.011.7898038
TAAGTCG10250.011.7800867
CCGGGGT21800.011.56324148
CACGATT8150.011.324086
GGACGCG18700.011.20543268
GTTACGC3205.74758E-511.09373555
CCTCGAA28000.010.90332222
AAGGCGG34050.010.84286518
Produced by FastQC (version 0.11.5)