##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6961622 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.09730993725313 39.0 36.0 41.0 28.0 41.0 2 35.96141890496209 39.0 35.0 41.0 27.0 41.0 3 35.81902967440634 39.0 35.0 41.0 27.0 41.0 4 35.719473852501615 39.0 35.0 40.0 27.0 41.0 5 35.644321538859764 39.0 35.0 40.0 27.0 41.0 6 35.56204918911139 39.0 35.0 40.0 27.0 41.0 7 35.41867556152862 39.0 35.0 40.0 27.0 41.0 8 35.298379458120536 38.0 34.0 40.0 26.0 41.0 9 35.47110170589555 39.0 35.0 40.0 27.0 41.0 10-11 35.703542579588486 39.0 35.0 40.5 27.0 41.0 12-13 35.62443034109005 39.0 35.0 41.0 27.0 41.0 14-15 35.473460998026034 39.0 35.0 41.0 26.0 41.0 16-17 35.33263605809106 39.0 35.0 41.0 26.0 41.0 18-19 35.19603384096408 39.0 35.0 41.0 25.0 41.0 20-21 35.02575355283582 39.0 34.0 41.0 24.5 41.0 22-23 34.80238499016465 39.0 34.0 40.5 24.0 41.0 24-25 34.604711373297775 38.5 34.0 40.0 24.0 41.0 26-27 33.6623393944687 37.5 33.0 39.5 22.0 40.5 28-29 33.680979159741796 38.0 33.0 39.5 22.0 40.5 30-31 34.10423483205494 38.0 33.0 40.0 22.0 41.0 32-33 33.984833347745685 38.0 33.0 40.0 21.5 41.0 34-35 33.731322097063014 38.0 33.0 40.0 20.5 41.0 36-37 33.42570754344318 37.5 33.0 40.0 19.0 41.0 38-39 33.11949873176108 37.0 32.0 40.0 18.0 41.0 40-41 32.76489415828667 36.5 32.0 40.0 15.5 41.0 42-43 32.39098761754086 36.0 31.5 39.0 13.0 41.0 44-45 32.02595135156721 35.5 31.0 39.0 9.5 40.5 46-47 31.604292864507727 35.0 31.0 38.5 7.5 40.0 48-49 31.18137504449394 35.0 31.0 37.5 6.0 40.0 50-51 30.72077972633389 35.0 30.0 37.0 2.0 39.0 52-53 30.22655388356334 35.0 30.0 36.5 2.0 39.0 54-55 29.747132636618304 34.0 29.0 36.0 2.0 38.5 56-57 29.295071249200255 34.0 29.0 35.5 2.0 37.0 58-59 28.85607492047112 34.0 29.0 35.0 2.0 37.0 60-61 28.487570209930965 34.0 29.0 35.0 2.0 36.5 62-63 28.109994912105254 34.0 27.5 35.0 2.0 36.0 64-65 27.71267033745871 34.0 27.0 35.0 2.0 36.0 66-67 27.349102982034935 33.0 26.0 35.0 2.0 35.0 68-69 26.974486621077673 33.0 26.0 35.0 2.0 35.0 70-71 26.513590223657648 33.0 25.0 35.0 2.0 35.0 72-73 26.01996934047841 33.0 24.5 35.0 2.0 35.0 74-75 25.428709214605448 32.5 21.5 35.0 2.0 35.0 76-77 23.65948819111408 30.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1465177953179833 1101 2 1.0916359371151145 1101 3 1.2159382154565037 1101 4 1.056190771813533 1101 5 1.1427510910641558 1101 6 1.0466036460672186 1101 7 1.0292953342774211 1101 8 1.027171332519842 1101 9 1.0133934117499876 1101 10-11 1.0551140117446636 1101 12-13 1.2781701485181998 1101 14-15 1.3399429865984445 1101 16-17 1.2688024938166862 1101 18-19 1.3078476797891838 1101 20-21 1.2900317861367796 1101 22-23 1.2252960891250737 1101 24-25 1.2207754175912555 1101 26-27 1.2149851792013067 1101 28-29 1.2503088335876456 1101 30-31 1.2071555390943374 1101 32-33 1.353279906965632 1101 34-35 1.3607597219326095 1101 36-37 1.3230106101575316 1101 38-39 1.3261589685987332 1101 40-41 1.214950585321347 1101 42-43 1.2291322370861693 1101 44-45 1.1799942712484253 1101 46-47 1.2135842056036807 1101 48-49 1.1970886315423002 1101 50-51 1.1734884755591963 1101 52-53 1.2308872069780925 1101 54-55 1.3053335772000239 1101 56-57 1.281353471621557 1101 58-59 1.289877713890533 1101 60-61 1.193330414183027 1101 62-63 1.1848288222549677 1101 64-65 1.144112958256681 1101 66-67 1.175624432366071 1101 68-69 1.1593341545050322 1101 70-71 1.2753320898057012 1101 72-73 1.1946143359014734 1101 74-75 1.2229982797946413 1101 76-77 1.3592796128159996 1102 1 1.1585576853314663 1102 2 1.1958712088842844 1102 3 1.3022998225656224 1102 4 1.0600811138308686 1102 5 1.0615359688762354 1102 6 1.2285209223843978 1102 7 1.0788903926441407 1102 8 1.0437210826718868 1102 9 1.0603340151036988 1102 10-11 1.175664390232697 1102 12-13 1.3275846799594362 1102 14-15 1.2798477511185737 1102 16-17 1.2575334491141277 1102 18-19 1.2469307199074535 1102 20-21 1.2550182544904587 1102 22-23 1.1954245200940221 1102 24-25 1.2345264902217536 1102 26-27 1.0847447588057406 1102 28-29 1.2873622275341532 1102 30-31 1.3685783547953818 1102 32-33 1.2628226401112883 1102 34-35 1.2185585581757508 1102 36-37 1.2362028945745536 1102 38-39 1.3291235479839756 1102 40-41 1.3560462027408207 1102 42-43 1.2697553554164003 1102 44-45 1.2935282480004915 1102 46-47 1.2121910078572213 1102 48-49 1.1352134592789724 1102 50-51 1.1778104899429032 1102 52-53 1.1953611053368114 1102 54-55 1.2667603987319431 1102 56-57 1.3410489176216984 1102 58-59 1.3638610894404515 1102 60-61 1.0994243410914812 1102 62-63 1.1622756291038776 1102 64-65 1.19250668305963 1102 66-67 1.2793032463579586 1102 68-69 1.2567733704304018 1102 70-71 1.2622070353238826 1102 72-73 1.2539445710563406 1102 74-75 1.1768061999521358 1102 76-77 1.1575522683090007 1103 1 0.7580208955959336 1103 2 0.7706479645550175 1103 3 0.796069660048154 1103 4 0.7762905242309088 1103 5 0.7618509214131137 1103 6 0.7578490725827436 1103 7 0.7970242682733684 1103 8 0.7856977463285162 1103 9 0.8821489443923269 1103 10-11 0.9268022694543987 1103 12-13 0.9071793284582057 1103 14-15 0.9124101604198316 1103 16-17 0.8746896748717248 1103 18-19 0.8357800052074609 1103 20-21 0.7997368650774632 1103 22-23 0.7854510230930529 1103 24-25 0.9995707353023064 1103 26-27 1.0083786797078602 1103 28-29 0.9414737407991112 1103 30-31 0.9959145586751461 1103 32-33 0.9594706714743992 1103 34-35 1.0653374526850072 1103 36-37 1.1148852941849583 1103 38-39 1.0040906945433576 1103 40-41 0.9893909766577522 1103 42-43 0.9910048629206827 1103 44-45 0.9233966139914926 1103 46-47 0.8916908279892226 1103 48-49 0.9104519985061046 1103 50-51 0.8933377478980837 1103 52-53 1.089071720974804 1103 54-55 1.0857196482296594 1103 56-57 1.068856693792494 1103 58-59 1.0277714931523398 1103 60-61 0.923356451322423 1103 62-63 0.8194687583910394 1103 64-65 0.8510580604569533 1103 66-67 0.9158077014558117 1103 68-69 0.8357226354187901 1103 70-71 0.7963667213991599 1103 72-73 0.739695354766905 1103 74-75 0.8642159906445137 1103 76-77 0.9755067653760499 1104 1 0.7596688014878339 1104 2 0.7362809641844521 1104 3 0.8394170959492371 1104 4 0.8698536687623246 1104 5 0.8687284821238137 1104 6 0.870822344994501 1104 7 0.7775204549883341 1104 8 0.7168976398010614 1104 9 0.6865580818705652 1104 10-11 0.8553205663039734 1104 12-13 0.9117329983007991 1104 14-15 0.9996671209563317 1104 16-17 0.9764782747274765 1104 18-19 0.8150425489581181 1104 20-21 0.8175608805751295 1104 22-23 0.9013245150992262 1104 24-25 0.9372066549593683 1104 26-27 0.7378438057069161 1104 28-29 0.8066814361760635 1104 30-31 0.8853860867471255 1104 32-33 0.8701279904511594 1104 34-35 0.9895530684355336 1104 36-37 1.03674074381329 1104 38-39 1.00633658532238 1104 40-41 0.9944671191505492 1104 42-43 1.0483228919352072 1104 44-45 0.9479804318636553 1104 46-47 0.8447400817757327 1104 48-49 0.7986481113910955 1104 50-51 0.6452432531121488 1104 52-53 0.6744247163962065 1104 54-55 0.5905708414983373 1104 56-57 0.5795336312606842 1104 58-59 0.5767844555729802 1104 60-61 0.592997167558476 1104 62-63 0.5747313002461887 1104 64-65 0.5020319953482009 1104 66-67 0.5955585987411602 1104 68-69 0.389211196511436 1104 70-71 0.32281848976448657 1104 72-73 0.31705319889372774 1104 74-75 0.2542551281132752 1104 76-77 0.3241050383801536 1105 1 0.14106778237416506 1105 2 0.3894265581348435 1105 3 0.2978198301830375 1105 4 0.2618251845458701 1105 5 0.16487572940374662 1105 6 0.3399906867999576 1105 7 0.38508509695353155 1105 8 0.3124254079355211 1105 9 0.2444818564392861 1105 10-11 0.4048375039305583 1105 12-13 0.47549901577636433 1105 14-15 0.45743112610964687 1105 16-17 0.38068166338567977 1105 18-19 0.3169046143903742 1105 20-21 0.26366798827766047 1105 22-23 0.284268180579474 1105 24-25 0.32526022188243076 1105 26-27 0.09829979066398664 1105 28-29 0.30873225141792204 1105 30-31 0.5628811629237944 1105 32-33 0.4331274160719971 1105 34-35 0.3768206766106985 1105 36-37 0.34571672891163274 1105 38-39 0.34942554676201354 1105 40-41 0.5502656953892213 1105 42-43 0.5257893798714761 1105 44-45 0.36084953629585925 1105 46-47 0.26513759866713116 1105 48-49 0.19761199292652876 1105 50-51 0.28556121002669244 1105 52-53 0.2255935158520188 1105 54-55 0.01757252629191086 1105 56-57 0.10149310815230095 1105 58-59 0.2731717927024526 1105 60-61 0.34015767601177416 1105 62-63 0.13262007910268991 1105 64-65 0.2093649753303346 1105 66-67 0.25852039823811523 1105 68-69 0.1623705837259628 1105 70-71 0.011928920316776725 1105 72-73 -0.257510910705502 1105 74-75 -0.5325100576752 1105 76-77 -0.6022690398233834 1106 1 0.011914276847804217 1106 2 0.14002950192280395 1106 3 0.03067722905000636 1106 4 0.09101623818074245 1106 5 0.16277990289226096 1106 6 -0.007603816384516904 1106 7 -0.3083559311819499 1106 8 -0.051681932516508766 1106 9 0.03465193230353236 1106 10-11 -0.2634177443725605 1106 12-13 0.14351694703000106 1106 14-15 0.12703895968537893 1106 16-17 0.036523482632169646 1106 18-19 0.0982568786347855 1106 20-21 0.1466631651337167 1106 22-23 0.19758264233099254 1106 24-25 -0.006550564096194478 1106 26-27 0.015168725415207973 1106 28-29 0.17281084155959547 1106 30-31 0.2932210301969249 1106 32-33 0.19271142854251622 1106 34-35 0.16551686730632298 1106 36-37 0.14934682640261343 1106 38-39 0.25002251698725075 1106 40-41 0.24191292765880945 1106 42-43 0.29275226616694283 1106 44-45 0.23606353816432346 1106 46-47 0.257640675864657 1106 48-49 0.13780769009209237 1106 50-51 0.012205392528379377 1106 52-53 0.07062924141435545 1106 54-55 -0.012195321555349636 1106 56-57 0.03729742399040603 1106 58-59 -0.07077121788375607 1106 60-61 -0.3162019535948275 1106 62-63 -0.27978839230324937 1106 64-65 -0.16875920813037482 1106 66-67 -0.34581793216329615 1106 68-69 -0.14445211890073395 1106 70-71 -0.3077825566039323 1106 72-73 -0.3693727808044933 1106 74-75 -0.3419025097739308 1106 76-77 -0.3815575803763558 1107 1 -0.016753879178835973 1107 2 0.055815434669796105 1107 3 0.07020821498806384 1107 4 -0.04897563474970923 1107 5 -0.03766942812712415 1107 6 -0.18464115078837295 1107 7 -0.0638067854886799 1107 8 -0.09470304619198089 1107 9 -0.16465777483435318 1107 10-11 -0.07368642763401567 1107 12-13 -0.01762388497594003 1107 14-15 -0.23382796476042955 1107 16-17 -0.17643331628408276 1107 18-19 -0.0917429293397305 1107 20-21 -0.13164924861627014 1107 22-23 -0.12759587248019244 1107 24-25 -0.18219896440336925 1107 26-27 -0.4046815170966127 1107 28-29 -0.23838667240788425 1107 30-31 -0.02638721715118919 1107 32-33 0.05239186369465898 1107 34-35 -0.10198115345344405 1107 36-37 -0.06828940964991403 1107 38-39 -0.0031629102246668594 1107 40-41 -0.07500767333445424 1107 42-43 -0.009504390616008607 1107 44-45 -0.02665014185163983 1107 46-47 -0.06874942932914152 1107 48-49 -0.04264588959873805 1107 50-51 -0.06254229201456596 1107 52-53 -0.0719354285489473 1107 54-55 -0.1336145199014851 1107 56-57 -0.10409505095284999 1107 58-59 -0.15918080513919008 1107 60-61 -0.2273852378712462 1107 62-63 -0.16258501441139472 1107 64-65 -0.08947406044900319 1107 66-67 -0.009921403071263768 1107 68-69 -0.20944185199564558 1107 70-71 -0.25854976299137533 1107 72-73 -0.2695528972632104 1107 74-75 -0.2819515081328454 1107 76-77 -0.30341978495634336 1108 1 0.22714888434417446 1108 2 0.4462733178155318 1108 3 0.41953995987596215 1108 4 0.41152719234347757 1108 5 0.3857819386719399 1108 6 0.38523645641753745 1108 7 0.43342948929943503 1108 8 0.3559706277512902 1108 9 0.393625474749733 1108 10-11 0.40369114519870664 1108 12-13 0.5702601544634476 1108 14-15 0.4966429114483901 1108 16-17 0.47188330763852093 1108 18-19 0.4235890670789857 1108 20-21 0.37075701571431807 1108 22-23 0.4112735450116318 1108 24-25 0.482711171965164 1108 26-27 0.33098866695762297 1108 28-29 0.433634972183107 1108 30-31 0.4735875084497181 1108 32-33 0.534157173308067 1108 34-35 0.5350878371638927 1108 36-37 0.43754555944252616 1108 38-39 0.5809273205932826 1108 40-41 0.48874590653143457 1108 42-43 0.5048257286225422 1108 44-45 0.45294496631874637 1108 46-47 0.4755197118831731 1108 48-49 0.5243412160094252 1108 50-51 0.42335536163153975 1108 52-53 0.5845210683465503 1108 54-55 0.6048762264333227 1108 56-57 0.6553036488511808 1108 58-59 0.6584706532138576 1108 60-61 0.6782311177787754 1108 62-63 0.5784603064278961 1108 64-65 0.6225293987063587 1108 66-67 0.7164139950793746 1108 68-69 0.7155355223622841 1108 70-71 0.7957893458454457 1108 72-73 0.7939456538060377 1108 74-75 0.8139876036155833 1108 76-77 0.7518741300197682 1109 1 -0.15609438907214468 1109 2 -0.26177695226454034 1109 3 -0.34127565303485596 1109 4 -0.21607520034965688 1109 5 -0.19553464062614268 1109 6 -0.31918131394412796 1109 7 -0.30957828266706144 1109 8 -0.17334424903059897 1109 9 -0.2395029186949813 1109 10-11 -0.24673602887360602 1109 12-13 -0.21771298405737838 1109 14-15 -0.13774786585790366 1109 16-17 -0.08954174227570633 1109 18-19 -0.21832393115370508 1109 20-21 -0.12580256587338567 1109 22-23 -0.0430281492312119 1109 24-25 -0.23584784942693204 1109 26-27 -0.442389980419172 1109 28-29 -0.15372872933430415 1109 30-31 -0.0713058899141501 1109 32-33 -0.10653126921855716 1109 34-35 -0.12818805119968602 1109 36-37 -0.07266948789001759 1109 38-39 -0.13451419229371453 1109 40-41 -0.0760698220069429 1109 42-43 -0.040602048507324184 1109 44-45 -0.05251031222905311 1109 46-47 -0.09392121887884386 1109 48-49 -0.11295565078263792 1109 50-51 -0.16972472740168598 1109 52-53 -0.20594219946782388 1109 54-55 -0.0679263265435388 1109 56-57 -0.11765764282831626 1109 58-59 -0.006958300127433148 1109 60-61 -0.11720612884983339 1109 62-63 -0.2093908250775236 1109 64-65 -0.0993343282291832 1109 66-67 -0.08072526934989455 1109 68-69 -0.1311862866907596 1109 70-71 -0.1331912576307559 1109 72-73 -0.21779353426917325 1109 74-75 -0.22451358405891497 1109 76-77 -0.1934242638535686 1110 1 -0.36802668483120726 1110 2 -0.3857897440451836 1110 3 -0.6103487202668063 1110 4 -0.4828701986722237 1110 5 -0.5130008319023815 1110 6 -0.449143345170981 1110 7 -0.4168324699711121 1110 8 -0.705234761320682 1110 9 -0.6610536893076073 1110 10-11 -0.6534005764776936 1110 12-13 -0.4926134925365986 1110 14-15 -0.4652643436770134 1110 16-17 -0.588350727560865 1110 18-19 -0.6630498123992155 1110 20-21 -0.7116752872344776 1110 22-23 -0.6785680474817255 1110 24-25 -0.7090262459479959 1110 26-27 -0.7334841122060851 1110 28-29 -0.5749679345210694 1110 30-31 -0.4341364402055845 1110 32-33 -0.4493361416829842 1110 34-35 -0.44447916862966963 1110 36-37 -0.35475495968511694 1110 38-39 -0.4179208114711557 1110 40-41 -0.3726264794367182 1110 42-43 -0.3567155363789496 1110 44-45 -0.40489191056553153 1110 46-47 -0.4613554613589983 1110 48-49 -0.47437277100724984 1110 50-51 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-1.1379433503477117 2314 36-37 -1.7238675250210775 2314 38-39 -1.298161037428521 2314 40-41 -1.1075859094107408 2314 42-43 -0.9457884816932172 2314 44-45 -1.0178024071606941 2314 46-47 -0.9003231131765403 2314 48-49 -0.795819204058013 2314 50-51 -0.6601514385601384 2314 52-53 -0.5879291007240361 2314 54-55 -0.7609549620251705 2314 56-57 -0.810331161393762 2314 58-59 -0.7171160719300289 2314 60-61 -0.658234798071792 2314 62-63 -0.7110454812678775 2314 64-65 -0.6274972663911775 2314 66-67 -0.5619886962524241 2314 68-69 -0.5211563958304843 2314 70-71 -0.4743084611235986 2314 72-73 -0.4822253531674683 2314 74-75 -0.40596898147989435 2314 76-77 -0.39458624314280044 2315 1 -1.559230210374274 2315 2 -1.4270383833176083 2315 3 -1.2026217428754862 2315 4 -1.2164847772912992 2315 5 -1.395235120660601 2315 6 -1.4016466247256432 2315 7 -1.7270885943602536 2315 8 -1.7824483961179425 2315 9 -1.3385528682874366 2315 10-11 -1.5379537514121466 2315 12-13 -1.4474888842719267 2315 14-15 -1.4049808821223309 2315 16-17 -1.2880158300018323 2315 18-19 -1.316656637862387 2315 20-21 -1.331461603424529 2315 22-23 -1.4788239865508856 2315 24-25 -1.6205174407638268 2315 26-27 -1.4555669000716236 2315 28-29 -1.4662840954402938 2315 30-31 -1.5408399806104498 2315 32-33 -1.398830818868582 2315 34-35 -1.5907840824695398 2315 36-37 -1.6784887181418284 2315 38-39 -1.5804085208559826 2315 40-41 -1.6975434878508722 2315 42-43 -1.7804609160112044 2315 44-45 -1.7280393929771662 2315 46-47 -1.6003177952215282 2315 48-49 -1.5016237775695842 2315 50-51 -1.4278615095666218 2315 52-53 -1.4563003678760502 2315 54-55 -1.5453499595231115 2315 56-57 -1.7104217979080367 2315 58-59 -1.557733820397754 2315 60-61 -1.5635193625324497 2315 62-63 -1.4853994829389983 2315 64-65 -1.4262033228905473 2315 66-67 -1.5415966662114364 2315 68-69 -1.6713888298404882 2315 70-71 -1.5610178273729574 2315 72-73 -1.524057005009194 2315 74-75 -1.506897590400353 2315 76-77 -1.4958264640266172 2316 1 -2.415545983384959 2316 2 -2.0482993438165096 2316 3 -2.4179574645822797 2316 4 -3.16457525150971 2316 5 -2.5813448541290853 2316 6 -2.5407954399874555 2316 7 -2.2808610721238765 2316 8 -2.0186799944100144 2316 9 -2.378426089835898 2316 10-11 -2.1857514291784526 2316 12-13 -2.8234027250370346 2316 14-15 -4.1188208652364615 2316 16-17 -3.09611228258607 2316 18-19 -2.660514913140851 2316 20-21 -2.4608836827726037 2316 22-23 -2.837570932578913 2316 24-25 -2.7155002262628685 2316 26-27 -2.668950079940526 2316 28-29 -3.164574858323853 2316 30-31 -3.4015597453508875 2316 32-33 -3.460174843139569 2316 34-35 -3.371756273132 2316 36-37 -3.403516470801055 2316 38-39 -3.4807791885846093 2316 40-41 -3.1784065856971466 2316 42-43 -3.338982165077013 2316 44-45 -3.2504569884054746 2316 46-47 -3.2965830275974177 2316 48-49 -3.0486487416173134 2316 50-51 -3.2187692475579937 2316 52-53 -3.2324193896071733 2316 54-55 -3.2130245726565043 2316 56-57 -3.1460991815572292 2316 58-59 -3.32954652676948 2316 60-61 -3.0529646838642 2316 62-63 -3.076935789498716 2316 64-65 -3.051813390224634 2316 66-67 -3.04295856935396 2316 68-69 -3.13464874085361 2316 70-71 -3.0558701428983177 2316 72-73 -3.036939291144556 2316 74-75 -3.1616719590350293 2316 76-77 -3.5486176864762378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 212577.0 3 16864.0 4 20344.0 5 25106.0 6 27834.0 7 29877.0 8 33007.0 9 32716.0 10 35065.0 11 37507.0 12 34418.0 13 33360.0 14 33830.0 15 35588.0 16 37284.0 17 39035.0 18 41396.0 19 44286.0 20 47007.0 21 51115.0 22 56377.0 23 64654.0 24 74025.0 25 86946.0 26 101081.0 27 118951.0 28 139471.0 29 163648.0 30 192330.0 31 229988.0 32 277396.0 33 346155.0 34 450532.0 35 597380.0 36 839062.0 37 1114520.0 38 989523.0 39 249848.0 40 1519.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.64083021481807 26.81333684030133 28.24438950960722 22.301443435273384 2 22.32046786314055 26.924627317012906 28.194845288255582 22.560059531590962 3 22.41766034251091 27.22362221081544 27.647152880047337 22.711564566626315 4 22.30081156789193 27.227372194974564 27.978669098769853 22.493147138363657 5 22.41819869763839 27.398641436223905 27.851792938123314 22.331366928014393 6 22.716155659428626 27.06633535575505 27.68648019317786 22.53102879163846 7 22.480541525659326 26.944306559957244 27.956671625719164 22.618480288664266 8 22.44093509631168 27.048746836867032 27.53618503428101 22.974133032540276 9 22.319613571101105 26.931566218877816 27.88307023110479 22.865749978916288 10-11 22.77203973461788 27.07174791313372 27.69207869395698 22.464133658291424 12-13 22.853261781199578 26.7489053570927 27.721571665194116 22.676261196513607 14-15 22.47621861933026 27.123257778716976 27.749847615569745 22.650675986383018 16-17 22.87734409129036 26.90636333847758 27.840832285018628 22.375460285213425 18-19 22.723395837391756 26.8720545889081 27.611923466576105 22.792626107124036 20-21 22.72477369991481 27.026493738588027 27.658805124057412 22.58992743743975 22-23 22.87842286197327 27.079864133554022 27.327326335049595 22.71438666942311 24-25 22.671236227507464 27.1540432273061 27.376482096666656 22.798238448519776 26-27 22.80826856546113 27.180166384436344 27.408665914722913 22.602899135379616 28-29 22.980592808993155 27.04333325480087 27.32895917097302 22.64711476523295 30-31 22.731269008537115 27.072969366401487 27.40092431896086 22.794837306100533 32-33 22.659169761685135 27.359742976392337 27.31793813947214 22.663149122450392 34-35 22.637374146083957 27.02036986130255 27.502254231321306 22.840001761292186 36-37 22.550910061913445 27.1624794253352 27.585483400021044 22.70112711273031 38-39 22.58808981557946 27.070370708793007 27.734865947282312 22.606673528345226 40-41 22.483874471281776 27.149689787035847 27.4778032445578 22.888632497124572 42-43 22.45164183129301 27.262291816484524 27.333952969566784 22.952113382655686 44-45 22.761552455023303 26.873568146116117 27.612191399839837 22.752687999020743 46-47 22.57064186058807 26.790480119480875 27.545158933456044 23.09371908647501 48-49 22.572303118913563 26.87593893740839 27.508055628624927 23.043702315053125 50-51 22.478702557374195 27.200092761092986 27.300907229639527 23.020297451893292 52-53 22.560660021961265 26.921704150541288 27.454815169883695 23.06282065761375 54-55 22.59231984043496 26.95927345721837 27.44415686932056 23.004249833026112 56-57 22.61333649018923 26.971083449033635 27.39258261461226 23.02299744616488 58-59 22.66973160810653 26.983109897905123 27.500440884642668 22.846717609345678 60-61 22.54389466028638 27.01959401659668 27.35332791393793 23.083183409179007 62-63 22.556673532963867 26.947298576741808 27.458488452429336 23.03753943786499 64-65 22.527464317543156 26.877165971788507 27.338215080486762 23.257154630181574 66-67 22.36942705175132 26.994475819976056 27.49542273708792 23.140674391184703 68-69 22.482605817231136 26.743378643762032 27.647965951980595 23.126049587026234 70-71 22.256191900962353 26.704958249249515 27.742392429603502 23.29645742018463 72-73 22.415490198389506 26.347396236547237 27.797140829210697 23.439972735852553 74-75 22.156034708241652 26.46852388626446 27.670544118051893 23.70489728744199 76-77 22.188926387831224 26.286106108789735 27.97263850999624 23.5523289933828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5435.0 1 3378.5 2 1306.5 3 1357.0 4 1423.0 5 1527.0 6 1668.0 7 1800.0 8 2013.5 9 2132.0 10 2248.0 11 2581.0 12 2991.5 13 3185.0 14 3448.5 15 4142.5 16 5053.5 17 5534.0 18 6163.5 19 7744.5 20 10060.0 21 13413.5 22 15403.0 23 19127.0 24 27363.5 25 38445.0 26 45014.0 27 51818.0 28 71894.5 29 92964.0 30 100761.0 31 116952.0 32 148181.5 33 179917.5 34 213008.5 35 229402.0 36 243328.5 37 268410.0 38 284809.0 39 290053.0 40 297677.5 41 310920.0 42 323699.5 43 330861.0 44 330410.0 45 334624.5 46 339144.5 47 340479.5 48 341960.0 49 339572.5 50 331173.5 51 315626.0 52 306090.0 53 298491.0 54 276871.5 55 245114.5 56 214839.0 57 202300.0 58 189412.0 59 159617.0 60 129426.0 61 116142.0 62 101755.0 63 75892.0 64 55393.0 65 46370.0 66 38233.5 67 25954.5 68 18501.5 69 12726.5 70 10262.0 71 8421.0 72 5483.5 73 3638.5 74 2890.0 75 2488.5 76 1703.5 77 1110.5 78 901.0 79 775.5 80 541.0 81 365.5 82 249.5 83 200.0 84 197.0 85 167.5 86 145.5 87 150.0 88 124.0 89 95.5 90 84.0 91 75.0 92 78.5 93 76.0 94 67.0 95 65.0 96 66.0 97 65.0 98 57.5 99 130.0 100 209.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034905658480164537 2 0.008791054728337735 3 0.012224162702312765 4 0.019980975697904885 5 0.014881589376728585 6 0.014220823825252218 7 0.018487070972827886 8 0.019463854831532075 9 0.018285968413682904 10-11 0.022193103848499672 12-13 0.02119477328702995 14-15 0.008747961322806669 16-17 0.006449646361149744 18-19 0.011405387997222486 20-21 0.010514790949580428 22-23 0.013832983175472612 24-25 0.021654436279361335 26-27 0.014292646167803996 28-29 0.00853249429515133 30-31 0.008690503448765244 32-33 0.010126950299800822 34-35 0.011829139818277982 36-37 0.009976123380442086 38-39 0.017610838393696183 40-41 0.013215311029527313 42-43 0.01560699503650155 44-45 0.017811940952841165 46-47 0.016914161670943926 48-49 0.0138042542384519 50-51 0.025963776832468064 52-53 0.030107925997705707 54-55 0.024441143170370355 56-57 0.015944560046494912 58-59 0.017014712950516417 60-61 0.020627376780870895 62-63 0.025590300651198813 64-65 0.02023953613109129 66-67 0.020160531554284333 68-69 0.029296333526870607 70-71 0.0543407843746759 72-73 0.064640108296601 74-75 0.07600958512254759 76-77 0.07368254122386995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 6961622.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.56779728383523 #Duplication Level Percentage of deduplicated Percentage of total 1 66.98141647691898 28.512513583736283 2 14.332654847384015 12.202190921652424 3 6.4652067447967765 8.256288303317803 4 3.58929844267693 6.1115411399622825 5 2.1951394376066657 4.67211252948963 6 1.4578537048144147 3.72345725856169 7 1.014879829347183 3.024083919017293 8 0.708080157635538 2.411313008874854 9 0.5559744447007925 2.129994671131458 >10 2.509237738717706 17.9800172744907 >50 0.11982942410864338 3.5199341091166083 >100 0.06551029594248933 5.000777748872506 >500 0.0031546831670137415 0.8988061765055273 >1k 0.0017298534872301492 1.4704975284285478 >5k 3.3918695676062014E-5 0.08647182684245859 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.723736221242693E-4 1.867380906346251E-4 3 0.0 0.0 0.0 3.447472442485386E-4 3.016538387174713E-4 4 1.4364468510355776E-5 0.0 0.0 7.613168310488562E-4 3.160183072278271E-4 5 5.74578740414231E-5 0.0 0.0 0.001738100689753049 3.3038277573818283E-4 6 5.74578740414231E-5 0.0 0.0 0.0036054815960993 3.3038277573818283E-4 7 5.74578740414231E-5 0.0 0.0 0.008862877070889515 4.0220511828996174E-4 8 5.74578740414231E-5 0.0 0.0 0.014651757880562891 4.883919293520964E-4 9 7.182234255177888E-5 1.4364468510355776E-5 0.0 0.021517973828512952 4.883919293520964E-4 10 1.0055127957249044E-4 1.4364468510355776E-5 0.0 0.029131142139001514 7.038589570074331E-4 11 1.723736221242693E-4 1.4364468510355776E-5 0.0 0.037692365371173556 7.038589570074331E-4 12 1.867380906346251E-4 1.4364468510355776E-5 0.0 0.04918394017945818 7.325878940281446E-4 13 1.867380906346251E-4 1.4364468510355776E-5 0.0 0.06511413575744274 7.469523625385004E-4 14 2.8728937020711554E-4 1.4364468510355776E-5 0.0 0.08562659679023078 8.044102365799235E-4 15 2.8728937020711554E-4 1.4364468510355776E-5 0.0 0.10720202849278516 8.618681106213466E-4 16 2.8728937020711554E-4 1.4364468510355776E-5 0.0 0.12808796570684244 8.618681106213466E-4 17 2.8728937020711554E-4 1.4364468510355776E-5 0.0 0.14953411719280363 8.618681106213466E-4 18 2.8728937020711554E-4 1.4364468510355776E-5 0.0 0.17125319358046157 8.905970476420582E-4 19 3.016538387174713E-4 1.4364468510355776E-5 0.0 0.19623300431997026 9.193259846627696E-4 20 3.160183072278271E-4 1.4364468510355776E-5 0.0 0.22283600000114917 9.193259846627696E-4 21 3.160183072278271E-4 1.4364468510355776E-5 0.0 0.2525129919435442 0.0011635219493388179 22 4.165695868003175E-4 1.4364468510355776E-5 0.0 0.2843446541624926 0.0012066153548698853 23 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.31680835299589666 0.0012784376974216641 24 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.3496311635420596 0.0014364468510355776 25 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.38294236601757464 0.001479540256566645 26 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.41823586514751876 0.0016519138786909144 27 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.4539172049272425 0.0016519138786909144 28 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.4889377791554899 0.001982296654429097 29 4.740274608417406E-4 1.4364468510355776E-5 0.0 0.5267019668692152 0.002068483465491232 30 5.027563978624522E-4 1.4364468510355776E-5 0.0 0.5687467661990266 0.0021259413395326547 31 5.027563978624522E-4 1.4364468510355776E-5 0.0 0.6147417943691859 0.002183399213574078 32 5.314853348831637E-4 1.4364468510355776E-5 0.0 0.6643997620094857 0.002197763682084434 33 5.314853348831637E-4 1.4364468510355776E-5 0.0 0.7152643450046555 0.0023414083671879915 34 5.458498033935195E-4 1.4364468510355776E-5 0.0 0.7671057118585295 0.0023414083671879915 35 5.458498033935195E-4 1.4364468510355776E-5 0.0 0.820139329598763 0.0024563241152708378 36 5.602142719038752E-4 1.4364468510355776E-5 0.0 0.8747817678121564 0.0025568753948433283 37 6.033076774349426E-4 1.4364468510355776E-5 0.0 0.9324551088812348 0.0025856043318640397 38 6.176721459452984E-4 1.4364468510355776E-5 0.0 0.9908179444388103 0.0028154358280297323 39 6.176721459452984E-4 1.4364468510355776E-5 0.0 1.0526713458444024 0.0028154358280297323 40 6.176721459452984E-4 1.4364468510355776E-5 0.0 1.1186904431179976 0.0029016226390918666 41 6.176721459452984E-4 1.4364468510355776E-5 0.0 1.1902829541736106 0.0029734449816436457 42 6.607655514763657E-4 1.4364468510355776E-5 0.0 1.2698621097209817 0.0029734449816436457 43 6.607655514763657E-4 1.4364468510355776E-5 0.0 1.3554456131056813 0.0031027251982368477 44 6.607655514763657E-4 1.4364468510355776E-5 0.0 1.4441174772201075 0.003131454135257559 45 6.607655514763657E-4 1.4364468510355776E-5 0.0 1.5359638888753224 0.003145818603767915 46 6.751300199867215E-4 1.4364468510355776E-5 0.0 1.6328522289776721 0.0031745475407886268 47 7.038589570074331E-4 1.4364468510355776E-5 0.0 1.730918455497871 0.0032463698833404054 48 7.038589570074331E-4 1.4364468510355776E-5 0.0 1.8318432112516307 0.003404379036954319 49 7.613168310488562E-4 1.4364468510355776E-5 0.0 1.9362872617904276 0.003777855218223569 50 7.613168310488562E-4 1.4364468510355776E-5 0.0 2.044480438610427 0.003792219686733925 51 7.900457680695677E-4 1.4364468510355776E-5 0.0 2.1615795859068476 0.003864042029285704 52 7.900457680695677E-4 1.4364468510355776E-5 0.0 2.2896100937396486 0.003921499903327127 53 7.900457680695677E-4 1.4364468510355776E-5 0.0 2.430008408959866 0.003921499903327127 54 7.900457680695677E-4 1.4364468510355776E-5 0.0 2.5743856819574518 0.00397895777736855 55 7.900457680695677E-4 1.4364468510355776E-5 0.0 2.726476674545099 0.0040364156514099735 56 9.336904531731255E-4 1.4364468510355776E-5 0.0 2.8842416321943363 0.004050780119920329 57 9.336904531731255E-4 1.4364468510355776E-5 0.0 3.0451524084473416 0.0041944248050238865 58 9.767838587041928E-4 1.4364468510355776E-5 0.0 3.21748293716608 0.0043524339586378 59 9.767838587041928E-4 1.4364468510355776E-5 0.0 3.386337264505312 0.0043524339586378 60 9.767838587041928E-4 1.4364468510355776E-5 0.0 3.564255571474579 0.004381162895658512 61 0.0010629706697663274 1.4364468510355776E-5 0.0 3.758477550203099 0.004424256301189579 62 0.0010629706697663274 1.4364468510355776E-5 0.0 3.9624242741131304 0.004424256301189579 63 0.0010629706697663274 1.4364468510355776E-5 0.0 4.17519078168852 0.004855190356500253 64 0.0010629706697663274 1.4364468510355776E-5 0.0 4.396575970370123 0.004855190356500253 65 0.0010629706697663274 2.872893702071155E-5 0.0 4.629222902363845 0.004883919293520964 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGCG 2430 0.0 15.045792 17 CGCACGA 625 0.0 14.197635 4 GCCTCGA 2175 0.0 13.383986 21 CGAACTC 2180 0.0 13.188359 25 TCGAACT 2235 0.0 12.547631 24 ACGCTCG 680 0.0 12.530206 45 CTCGAAC 2275 0.0 12.327191 23 GGCGGAA 3080 0.0 12.102344 20 CGGGGTG 2065 0.0 12.035612 49 GACGCGA 1745 0.0 12.009494 69 CGTGCGC 1160 0.0 11.936701 46 ACGCACG 750 0.0 11.831107 3 GTAGACC 4185 0.0 11.789803 8 TAAGTCG 1025 0.0 11.78008 67 CCGGGGT 2180 0.0 11.563241 48 CACGATT 815 0.0 11.32408 6 GGACGCG 1870 0.0 11.205432 68 GTTACGC 320 5.74758E-5 11.093735 55 CCTCGAA 2800 0.0 10.903322 22 AAGGCGG 3405 0.0 10.842865 18 >>END_MODULE