FastQCFastQC Report
Thu 26 May 2016
SRR936084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961622
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.1041998545741208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA22500.035.448227100-101
GGTATCA121900.035.0534321
CGGTATC18750.028.728935100-101
CCTGTCG22100.028.54507696-97
GTATCAA221550.028.0439111
CGATCTA4700.025.961409100-101
CCGTACA13800.025.878817100-101
TCGTATA8000.024.552526100-101
TCGGTAT7250.023.808512100-101
CGTCCTA7400.023.728075100-101
TTATACG17650.023.60320396-97
TACGCAG14300.023.309032100-101
ATCAACG265850.022.8944513
GCGTATC7550.022.862478100-101
TAAATCG7200.022.73096396-97
TCAACGC270300.022.5395474
ACGTATC10950.022.286505100-101
ACCGTAT8500.022.05614398-99
GCGTACA11400.021.92889100-101
CAACGCA278050.021.8680445
Produced by FastQC (version 0.11.5)