FastQCFastQC Report
Thu 26 May 2016
SRR936083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773446
Sequences flagged as poor quality0
Sequence length77
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366852.0685715832339975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195721.10361409369104No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG27180.15326093943655458No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA24820.1399535142316146No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC23550.13279231507471895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG400.002254942435.5070163
CGGTATA1600.033.2756210
CGTACGG450.00402065631.55466314
TAGCGGC853.5534667E-629.2278756
TCTAGCG853.554247E-629.2270534
CTAGCGG905.543945E-627.6041035
CGGTTAC657.339973E-427.2999944
TCGCGAT807.874025E-526.62575126
TTCGCGA851.1889562E-425.0595325
GCGGTAT2200.024.2004499
ACGTACG750.001689291123.66399813
TCACGTA2003.6379788E-1123.08672371
GATCACG1904.6929927E-1022.43244269
ATGGCGG2600.021.8406096
CGTAAAT2051.2205419E-920.78107524
CGAATGT4050.020.15992558
ATCCGCC4250.020.04310236
AGCGGCG1257.034416E-519.8749547
TCGTAAA2705.456968E-1219.72333523
AATCCGC4350.019.58178935
Produced by FastQC (version 0.11.5)