FastQCFastQC Report
Thu 26 May 2016
SRR936083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773446
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237811.3409486389774483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166950.9413875584596316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129530.7303859266084223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49500.27911760493412263No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.2659793419139912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.2319777427674708No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31950.1801577268211155No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28620.16138072430736544No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.15889967893017323No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24720.13938964028225276No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG24560.13848744196327376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.11869546634067235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA19750.053.04346100-101
CCCGGTA4100.052.26427598-99
CGAGTAC14650.049.77196100-101
CGGTATC15250.049.7653100-101
TCGAGTA4900.048.59037100-101
TTACGCA5350.048.39737100-101
TACGCAG12250.048.347416100-101
CTACGCG1250.047.61117696-97
CCGTACA6850.047.357876100-101
CCCGTAC3100.047.04253498-99
ACGTATC5800.046.181793100-101
TCGTATC5900.045.903484100-101
CGTACAT24050.045.415833100-101
AGTATCA46100.044.67468100-101
CCGTATC7400.044.642403100-101
CCGGTAT5750.044.513004100-101
AGGTATC28600.044.018032100-101
TACGTAC3250.043.95559398-99
ACGTACA7200.043.402332100-101
ACCGTAC2100.042.51657598-99
Produced by FastQC (version 0.11.5)