FastQCFastQC Report
Thu 26 May 2016
SRR936082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779958
Sequences flagged as poor quality0
Sequence length77
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368582.070723017059953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195721.0995765068613979No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG28050.15758798803117827No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA23360.13123905170796163No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC23070.1296097997817926No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG350.001170000840.58082646
TAGCGGC751.3378976E-633.1270376
GTACGCT450.00402439831.54867427
CGGTATA1451.6370905E-1129.3753810
CTAGCGG853.5524881E-629.228925
ATGGCGG1701.1823431E-1025.0540626
GCTACGA1502.1304004E-823.6701545
TCGTCAC750.001688671123.66549556
CAATACG1051.8307757E-523.66416514
AGTCCGC750.00168977623.66283617
TCTAGCG1102.6255577E-522.5859814
TCGCGAT952.52995E-422.41805526
CCCGTAT1655.8709702E-821.510462
TTCGCGA1003.5786198E-421.29715225
CGCGTCA1003.580658E-421.2953572
AGCTACG1708.0179234E-820.88542744
CGCTCCG850.003495114620.87955910
GCGCAAT1205.1323907E-520.70614411
ACCGTTA1751.09172106E-720.28357111
GCGGTAT2302.3646862E-1020.0625349
Produced by FastQC (version 0.11.5)