FastQCFastQC Report
Thu 26 May 2016
SRR936082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779958
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236181.3268852411124308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164870.926257810577553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128820.72372494182447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51380.28865849643643277No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47520.2669725914881138No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.22938743498442096No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.18691452270222106No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28870.16219483830517348No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27650.15534074399508302No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG25300.14213818528302352No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24010.13489082326661642No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.11489035134536882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA20100.053.153767100-101
CCGTACA8100.050.335133100-101
CGAGTAC13000.049.676624100-101
TTACGCA5350.049.507603100-101
CGGTATC12550.048.849392100-101
TACGCAG13600.048.360302100-101
CTATCGT1000.047.61228696-97
AGACGCG1750.047.61228696-97
CTACGCG1200.047.11632596-97
CCCGGTA4300.047.05997598-99
CGTACAT22700.046.147957100-101
TCGAGTA5050.045.96623100-101
ACGTATC5900.045.396896100-101
CCGCGTA800.044.6377798-99
AGTATCA44250.043.782784100-101
ACGCGCT751.8189894E-1243.6458298-99
AGGTATC26800.043.41878100-101
ACCGTAC3050.042.93031798-99
CCGTATC7600.042.682373100-101
TCGTATC6650.042.514553100-101
Produced by FastQC (version 0.11.5)