FastQCFastQC Report
Thu 26 May 2016
SRR936081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948542
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184881.9490966135395165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA137201.446430416365327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC1553.6379788E-1127.4790764
GCCGTAT952.5159318E-422.43488171
AATCCGC1454.025569E-722.0306386
CGTATTA1303.8450416E-621.84349441
ACGGTTA850.003491549820.88201119
GACGTAT1459.887239E-619.58485439
ACGTATT1459.891741E-619.5838240
TAAGGCG3650.019.45173617
CCGATAT950.006645747518.6829239
ACCGCGA950.00664979418.680955
TGATCCG1551.7562583E-518.3222834
GAGACGT1602.3067798E-517.75064737
CCCGATA1000.00893278417.74877438
AAATCCG1653.0101599E-517.2091185
TCGCGCT1905.3452277E-616.81640237
CGATCGT1905.3532804E-616.8137441
ATTAAGG4450.016.75253515
TATTAAG4450.016.75253514
CGCGATC1703.8833467E-516.7056135
GATGTAT3850.016.59713254
Produced by FastQC (version 0.11.5)