##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936081_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 948542 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.158366208349236 39.0 35.0 41.0 29.0 41.0 2 35.98684823655674 39.0 35.0 41.0 28.0 41.0 3 35.87810766418356 39.0 35.0 41.0 28.0 41.0 4 35.73566167866051 39.0 35.0 41.0 27.0 41.0 5 35.62845503941839 39.0 35.0 40.0 27.0 41.0 6 35.48222746067122 38.0 35.0 40.0 27.0 41.0 7 35.248726993638655 38.0 35.0 40.0 27.0 41.0 8 35.002523873481614 38.0 34.0 40.0 26.0 41.0 9 34.85572172871628 38.0 34.0 40.0 25.0 41.0 10-11 34.99005051963961 38.0 34.0 40.0 26.0 41.0 12-13 35.05812974016965 38.0 35.0 40.0 25.5 41.0 14-15 34.91951384335116 38.0 34.5 41.0 25.0 41.0 16-17 34.65667255640762 38.0 34.0 40.5 24.0 41.0 18-19 34.3795135059913 38.0 34.0 40.0 23.0 41.0 20-21 34.031849933898556 38.0 33.0 40.0 20.5 41.0 22-23 33.58141389627449 38.0 33.0 40.0 16.5 41.0 24-25 33.19918517050378 38.0 33.0 40.0 10.5 41.0 26-27 32.1866902045455 37.0 31.5 39.5 7.5 40.5 28-29 32.1575744669187 37.0 31.5 39.5 4.0 40.5 30-31 32.457719320810256 37.0 32.0 40.0 2.0 41.0 32-33 32.24371561828575 37.0 32.0 40.0 2.0 41.0 34-35 31.927607844460233 36.0 31.0 40.0 2.0 41.0 36-37 31.602280658104753 36.0 31.0 39.5 2.0 41.0 38-39 31.279597002557608 35.0 31.0 39.0 2.0 41.0 40-41 30.8710014949259 35.0 30.5 39.0 2.0 41.0 42-43 30.48259328527361 35.0 30.0 38.5 2.0 40.0 44-45 30.093087074689365 35.0 29.5 38.0 2.0 40.0 46-47 29.692430593479255 35.0 29.0 37.0 2.0 40.0 48-49 29.234139869399563 35.0 29.0 36.5 2.0 39.0 50-51 28.797422781489907 34.0 28.0 36.0 2.0 39.0 52-53 28.34683229630317 34.0 27.5 35.5 2.0 38.5 54-55 27.88157140116094 34.0 26.5 35.0 2.0 37.0 56-57 27.430191283042817 34.0 26.0 35.0 2.0 37.0 58-59 27.01166579866785 34.0 25.0 35.0 2.0 36.0 60-61 26.655474401766078 33.0 25.0 35.0 2.0 36.0 62-63 26.273993138943766 33.0 24.0 35.0 2.0 35.5 64-65 25.872867516672958 33.0 22.0 35.0 2.0 35.0 66-67 25.458431993522687 33.0 19.5 35.0 2.0 35.0 68-69 25.05239673098292 33.0 17.5 35.0 2.0 35.0 70-71 24.583060634110034 32.0 7.5 35.0 2.0 35.0 72-73 24.04245568461913 32.0 2.0 35.0 2.0 35.0 74-75 23.42110839583276 32.0 2.0 34.5 2.0 35.0 76-77 21.822816490993546 29.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6880679943665342 1101 2 0.5876456343135885 1101 3 0.6106097603966916 1101 4 0.779077897096883 1101 5 0.6265639667657013 1101 6 0.8532083050877617 1101 7 0.9027772775087328 1101 8 0.896671942322179 1101 9 0.982478946673659 1101 10-11 1.0144516480053696 1101 12-13 0.9087605317474186 1101 14-15 1.0091464455590398 1101 16-17 1.0377833328271322 1101 18-19 0.9866238975158481 1101 20-21 1.008680293602005 1101 22-23 1.0200595875013576 1101 24-25 1.0076876783727613 1101 26-27 1.0655567686084026 1101 28-29 1.0173807069333947 1101 30-31 0.9825340218763827 1101 32-33 1.0699229806203974 1101 34-35 1.0166096718223088 1101 36-37 0.9894913117043167 1101 38-39 1.0366955773387616 1101 40-41 0.9864988397033052 1101 42-43 0.9743702014595463 1101 44-45 0.9880219918607409 1101 46-47 0.8907470316841817 1101 48-49 1.0051987359307688 1101 50-51 1.0781954955299824 1101 52-53 1.2066388394257288 1101 54-55 1.0060108613115268 1101 56-57 0.685657489476835 1101 58-59 0.7132934781941245 1101 60-61 0.6887964632136168 1101 62-63 0.5902221123522189 1101 64-65 0.5805729572783171 1101 66-67 0.607237784035874 1101 68-69 0.33897738140020195 1101 70-71 0.4792187698952688 1101 72-73 0.8070507146114068 1101 74-75 0.34829969944836847 1101 76-77 0.4278730467746037 1102 1 1.087476027681305 1102 2 1.1451111937160547 1102 3 1.0810991921570974 1102 4 1.2282371591359578 1102 5 1.2259514204615556 1102 6 1.2694401768646273 1102 7 1.1866670675966233 1102 8 1.3080312866797286 1102 9 1.0065540743883474 1102 10-11 1.167851522106531 1102 12-13 1.2699101455769224 1102 14-15 1.2214085693757113 1102 16-17 1.3182139310887564 1102 18-19 1.3284180771153942 1102 20-21 1.4398172570186958 1102 22-23 1.4589744363016877 1102 24-25 1.4530578694000482 1102 26-27 1.142857448946387 1102 28-29 1.1007932314473479 1102 30-31 1.130624051656298 1102 32-33 1.0716631689844363 1102 34-35 0.9260457297357831 1102 36-37 1.0509532515149864 1102 38-39 1.0158587131884644 1102 40-41 1.1807326120685069 1102 42-43 0.9942673638932682 1102 44-45 1.0882205246549717 1102 46-47 1.085796422769576 1102 48-49 1.2007005759908154 1102 50-51 1.3659923867969574 1102 52-53 1.2396085994915822 1102 54-55 1.1264727526995593 1102 56-57 1.214642974792671 1102 58-59 1.4856496024465997 1102 60-61 1.2252965358475656 1102 62-63 1.0998809143762855 1102 64-65 1.2896982024178953 1102 66-67 1.3329175167429383 1102 68-69 1.220800432994519 1102 70-71 1.4314498426986972 1102 72-73 1.565621520606129 1102 74-75 1.8255365224394318 1102 76-77 1.5795433249626285 1103 1 0.5983119863166948 1103 2 0.6528114291148839 1103 3 0.5561876131111063 1103 4 0.8058294741253604 1103 5 0.6293149301293042 1103 6 0.7276642039413517 1103 7 0.877170305794472 1103 8 0.7991789438172319 1103 9 0.5328475968215045 1103 10-11 0.747171266329886 1103 12-13 0.7830261644088239 1103 14-15 0.6784856127195411 1103 16-17 0.9415567701626202 1103 18-19 0.8586349134019429 1103 20-21 0.7422549817308948 1103 22-23 0.8881908275556256 1103 24-25 0.8864625445847949 1103 26-27 0.8453180108586196 1103 28-29 0.9339155158700407 1103 30-31 1.206665340142841 1103 32-33 1.2151261668646782 1103 34-35 1.3436539429298335 1103 36-37 1.3381537328528985 1103 38-39 1.2968075110148263 1103 40-41 1.3847778707144407 1103 42-43 1.1734420333394375 1103 44-45 1.099806011907127 1103 46-47 0.8771742086151946 1103 48-49 0.8886851345301707 1103 50-51 0.9241438032889313 1103 52-53 1.1045997573961834 1103 54-55 1.2144786845158784 1103 56-57 1.1810993733076671 1103 58-59 0.9094158080750887 1103 60-61 0.8573211707451485 1103 62-63 0.8084965408768063 1103 64-65 0.7335567523142501 1103 66-67 0.7482851956636196 1103 68-69 0.6133111489114604 1103 70-71 0.49025270496110096 1103 72-73 0.3899097612048159 1103 74-75 0.4072461210902816 1103 76-77 0.5112901072596969 1104 1 0.5541251392401989 1104 2 0.7873762605781138 1104 3 0.6568914618035535 1104 4 0.6246467579635819 1104 5 0.4899420780911612 1104 6 0.2814272624114338 1104 7 0.5282360362722045 1104 8 0.5912562363042042 1104 9 0.5754540560699795 1104 10-11 0.3424141709433144 1104 12-13 0.6682488980956975 1104 14-15 0.34558424257592435 1104 16-17 0.4258858948183786 1104 18-19 0.49178934474682734 1104 20-21 0.29260050486629297 1104 22-23 0.2511181439404453 1104 24-25 0.28938320371035786 1104 26-27 0.09171484041002742 1104 28-29 0.22421327174777161 1104 30-31 0.4011234563091648 1104 32-33 0.4037008590375635 1104 34-35 0.28034596787463784 1104 36-37 0.3690289063968315 1104 38-39 0.4108486380831309 1104 40-41 0.17299309933723706 1104 42-43 0.211569067501987 1104 44-45 0.1976556318174545 1104 46-47 -0.1127289481774838 1104 48-49 0.008163023325494123 1104 50-51 0.04440761852438513 1104 52-53 -0.026225344454193333 1104 54-55 -0.06763903517367353 1104 56-57 0.03742988631492494 1104 58-59 0.32427045791463627 1104 60-61 0.22795269998018952 1104 62-63 -0.1368840608939088 1104 64-65 -0.04037026250242093 1104 66-67 0.1726947195273283 1104 68-69 0.191765811268926 1104 70-71 0.0398087454281324 1104 72-73 0.12447501941543493 1104 74-75 0.33973326176898055 1104 76-77 0.0159900184962396 1105 1 -0.048480170106003584 1105 2 0.2950660367957596 1105 3 0.22750357237836738 1105 4 0.15234530996007578 1105 5 0.3978073698858111 1105 6 0.0057745638594326465 1105 7 0.12094117010283156 1105 8 0.5205453982173225 1105 9 0.03229916357327056 1105 10-11 0.1545302306186116 1105 12-13 0.22826699813957418 1105 14-15 0.08210793717882581 1105 16-17 0.1440380448841978 1105 18-19 0.39011371947258056 1105 20-21 0.5043408515095607 1105 22-23 0.5747962922511078 1105 24-25 0.6505065034032071 1105 26-27 0.4957023349251628 1105 28-29 0.5952586864033194 1105 30-31 0.6568743338870533 1105 32-33 0.6880607756676582 1105 34-35 0.6992084075411569 1105 36-37 0.8769325922239481 1105 38-39 0.822169392800113 1105 40-41 0.8306225420752824 1105 42-43 0.4002647673703521 1105 44-45 0.11200128780692253 1105 46-47 0.15902401364787622 1105 48-49 0.44586596321140703 1105 50-51 0.5458693341891454 1105 52-53 0.4307239754229464 1105 54-55 0.3306469455196108 1105 56-57 0.34206733695117464 1105 58-59 0.41643533285978407 1105 60-61 0.4521903041925661 1105 62-63 0.3034680672324619 1105 64-65 0.3913294961636602 1105 66-67 0.1834368213263673 1105 68-69 -0.34743449588333064 1105 70-71 -0.48357047791543906 1105 72-73 -0.39413237417824476 1105 74-75 -0.5386399282266332 1105 76-77 -0.4345145887876569 1106 1 -0.31439799774580024 1106 2 -0.14032342311318047 1106 3 -0.4017611294946377 1106 4 -0.5431821035462931 1106 5 -0.6565193565789258 1106 6 -0.3014475523378408 1106 7 -0.13563343662035976 1106 8 -0.19216078243100299 1106 9 -0.09224485343919042 1106 10-11 -0.28433465119387336 1106 12-13 -0.09800961790775631 1106 14-15 -0.02218130189530143 1106 16-17 -0.10903455796756845 1106 18-19 -0.04662460490921916 1106 20-21 -0.002291690271839286 1106 22-23 0.010039210542231558 1106 24-25 -0.08387488649292152 1106 26-27 -0.20242874825594726 1106 28-29 -0.009618763908839867 1106 30-31 -0.07521214611512761 1106 32-33 -0.0526055456606187 1106 34-35 -0.17695097521191627 1106 36-37 -0.07704516071369838 1106 38-39 -0.23870399524209063 1106 40-41 -0.11341696281852975 1106 42-43 -0.15132742434697732 1106 44-45 -0.32689631027372457 1106 46-47 -0.14139857148257917 1106 48-49 -0.3414244481956672 1106 50-51 -0.28757896047537557 1106 52-53 -0.1945643366902523 1106 54-55 -0.09527388111143154 1106 56-57 -0.4548580154302968 1106 58-59 -0.2822839615593651 1106 60-61 -0.4333191153663094 1106 62-63 -0.7246611074996743 1106 64-65 -0.6310981872519186 1106 66-67 -0.47485402629761353 1106 68-69 -0.6247212267102356 1106 70-71 -0.8509230502320229 1106 72-73 -0.9780564179089914 1106 74-75 -0.8763672381314827 1106 76-77 -0.5517287985534622 1107 1 -0.15445111948440626 1107 2 -0.31858494730042963 1107 3 -0.3411176718459714 1107 4 0.06461268800106978 1107 5 -0.3187716513788388 1107 6 -0.44175817552739005 1107 7 -0.07981700517095192 1107 8 -0.04761251667303412 1107 9 -0.14912809499768542 1107 10-11 -0.29004575283938294 1107 12-13 -0.042391363487055855 1107 14-15 0.052532081359764504 1107 16-17 0.20259495908530312 1107 18-19 0.2480292480254107 1107 20-21 0.20177467072176825 1107 22-23 0.27146044868538866 1107 24-25 0.1802687120673383 1107 26-27 0.0532994043352204 1107 28-29 0.3093066275873042 1107 30-31 0.304350912142489 1107 32-33 0.2566207532032081 1107 34-35 0.1822822083733655 1107 36-37 0.22817834822935978 1107 38-39 0.25962822924358164 1107 40-41 0.31165603453181845 1107 42-43 0.38825521997801715 1107 44-45 0.4666135207224542 1107 46-47 0.3246636123510669 1107 48-49 0.4155217210037634 1107 50-51 0.27983533117686576 1107 52-53 0.3443851706339309 1107 54-55 0.274716840600405 1107 56-57 0.34553975417732374 1107 58-59 0.2714432464737513 1107 60-61 0.42299417420507623 1107 62-63 0.4132694610496337 1107 64-65 0.32831734493705156 1107 66-67 0.3597151252601982 1107 68-69 0.26285320227611564 1107 70-71 0.4264089722160307 1107 72-73 0.553797322296365 1107 74-75 0.4729879787501119 1107 76-77 0.27666147635925853 1108 1 0.23872159793859993 1108 2 0.2706223178746683 1108 3 0.3056189975629593 1108 4 0.19304298437867828 1108 5 0.1275954054476145 1108 6 0.4408240389711935 1108 7 0.2293393819668097 1108 8 0.3701184556285426 1108 9 0.15457300957256592 1108 10-11 0.1339679221149126 1108 12-13 0.16528040915972042 1108 14-15 0.14571108148541612 1108 16-17 0.022776303963006228 1108 18-19 0.21493540815608725 1108 20-21 0.14371123676245645 1108 22-23 0.32683180382878874 1108 24-25 0.35194178392960396 1108 26-27 0.3954838407767909 1108 28-29 0.4263124811695107 1108 30-31 0.4139716265687525 1108 32-33 0.4629038114806079 1108 34-35 0.5313622073301971 1108 36-37 0.5949269666274795 1108 38-39 0.7731953107266776 1108 40-41 0.6221276402720797 1108 42-43 0.5936521800982639 1108 44-45 0.5941915032873588 1108 46-47 0.636838067593331 1108 48-49 0.5394907057901399 1108 50-51 0.5232639798267975 1108 52-53 0.5756609386054272 1108 54-55 0.6344869173916301 1108 56-57 0.560025295663312 1108 58-59 0.5593694330154939 1108 60-61 0.5173568118021912 1108 62-63 0.6877038399985445 1108 64-65 0.5138431834407733 1108 66-67 0.34472317423208665 1108 68-69 0.30987626809276136 1108 70-71 0.2480270407946925 1108 72-73 0.5901712071166436 1108 74-75 0.1495538534801355 1108 76-77 0.06261987301353855 1109 1 -0.25442470436791353 1109 2 -0.523109563253989 1109 3 -0.32250264575370835 1109 4 -0.42727787123629213 1109 5 -0.3017262702087038 1109 6 -0.22439581295936506 1109 7 -0.13752170764869476 1109 8 0.018387706387947844 1109 9 0.023239345142727075 1109 10-11 -0.1745177733609964 1109 12-13 -0.19716516203951073 1109 14-15 -0.006447590740592091 1109 16-17 -0.04063425955555289 1109 18-19 -0.23114918315146582 1109 20-21 0.07864442071736732 1109 22-23 0.19310558214043283 1109 24-25 -0.12053254646620815 1109 26-27 -0.13784448760934254 1109 28-29 -0.2440025995063877 1109 30-31 0.02971353299164292 1109 32-33 0.2375073619131527 1109 34-35 0.14944469655993942 1109 36-37 -0.011420846403087381 1109 38-39 0.014816451623641314 1109 40-41 0.09058088059039093 1109 42-43 0.22121365432471052 1109 44-45 0.2299499260359994 1109 46-47 0.2497092518023365 1109 48-49 0.11827300213691672 1109 50-51 0.15883413956161263 1109 52-53 -0.020793248802899456 1109 54-55 0.1718669430323594 1109 56-57 0.26648283253629756 1109 58-59 0.2851239288055609 1109 60-61 -0.007478891181143865 1109 62-63 0.061079060889966286 1109 64-65 0.011927058655889056 1109 66-67 0.13991611494656198 1109 68-69 0.154692105285676 1109 70-71 0.44460076944185545 1109 72-73 0.051863148766660316 1109 74-75 -0.2334291335493539 1109 76-77 -0.08871182793701493 1110 1 -0.3876699980507965 1110 2 -0.2926206714666719 1110 3 -0.3384682559445693 1110 4 -0.15350173106847365 1110 5 -0.1821510343493955 1110 6 -0.30091857283937173 1110 7 -0.3441562018011197 1110 8 -0.24261398919050237 1110 9 -0.24292299562507225 1110 10-11 -0.4117275685872883 1110 12-13 -0.07567750932875583 1110 14-15 -0.18271764421652392 1110 16-17 -0.2127425374565135 1110 18-19 -0.16081745844385154 1110 20-21 -0.49558115643145584 1110 22-23 -0.3598747230636974 1110 24-25 -0.21832503374937318 1110 26-27 -0.3074740689318993 1110 28-29 -0.08663770232800516 1110 30-31 -0.12423031727573886 1110 32-33 0.06077537199967509 1110 34-35 0.06762162365006219 1110 36-37 0.12499224433209832 1110 38-39 0.24452711637734836 1110 40-41 0.10848934109210973 1110 42-43 0.0884194279867252 1110 44-45 -0.042023575116125755 1110 46-47 0.0346479501197976 1110 48-49 0.2051257476697188 1110 50-51 0.19692813547483468 1110 52-53 0.11865884023670858 1110 54-55 -0.027367329390582285 1110 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-0.38267468889006295 2314 16-17 -0.8629866494337151 2314 18-19 -0.46248957310313443 2314 20-21 -0.323482212503869 2314 22-23 -0.1748001274723805 2314 24-25 -0.12779406400668591 2314 26-27 -0.28746317294665147 2314 28-29 -0.42260073979343105 2314 30-31 -0.49300179493896934 2314 32-33 -0.6862439474203264 2314 34-35 -0.7832238628975858 2314 36-37 -0.9343856368178471 2314 38-39 -0.523105521289235 2314 40-41 -0.3214672845050721 2314 42-43 -0.10029065499039191 2314 44-45 -0.30430775079293326 2314 46-47 -0.5163955500059245 2314 48-49 -0.36539432608772415 2314 50-51 -0.284255336153695 2314 52-53 -0.25843130308740214 2314 54-55 -0.40224684493831475 2314 56-57 -0.48159405520098986 2314 58-59 -0.5435183153460841 2314 60-61 -0.42977844830483747 2314 62-63 -0.4399987273012229 2314 64-65 -0.35876041033358064 2314 66-67 -0.46853712576865547 2314 68-69 -0.15539022192824703 2314 70-71 -0.0033998553628933337 2314 72-73 0.05898638231500186 2314 74-75 -0.004675251490439791 2314 76-77 0.007317905418368298 2315 1 -1.1821265366369786 2315 2 -1.06692461251302 2315 3 -1.0601591578622518 2315 4 -1.3225549358986157 2315 5 -1.2007114322584016 2315 6 -1.2597890974198336 2315 7 -1.2715702159759275 2315 8 -1.2208656694542768 2315 9 -0.9539105821040508 2315 10-11 -1.2767754402531821 2315 12-13 -1.4674123376389048 2315 14-15 -1.6555296026220674 2315 16-17 -1.5222712534109135 2315 18-19 -1.3273150419638782 2315 20-21 -1.2793528730481043 2315 22-23 -1.331627269878588 2315 24-25 -1.4010986314160263 2315 26-27 -1.4039994102685007 2315 28-29 -1.3251576611844378 2315 30-31 -1.526634996651822 2315 32-33 -1.7676483855914569 2315 34-35 -1.6553964048404453 2315 36-37 -1.8031306861606389 2315 38-39 -1.6363661357960737 2315 40-41 -1.7229655841590485 2315 42-43 -1.9573246083097935 2315 44-45 -1.8113562955230549 2315 46-47 -1.6646852282206481 2315 48-49 -1.544197919743734 2315 50-51 -1.7659862943627047 2315 52-53 -1.6017031738253706 2315 54-55 -1.3187988648064568 2315 56-57 -1.5026920439685796 2315 58-59 -1.3280902807793282 2315 60-61 -1.3144817364345762 2315 62-63 -1.4207469986657308 2315 64-65 -1.4925576909710792 2315 66-67 -1.2745979213281018 2315 68-69 -1.36903378652055 2315 70-71 -1.5348934798097602 2315 72-73 -2.6916516196741966 2315 74-75 -2.893129872203094 2315 76-77 -2.745332371222865 2316 1 -3.318307716455813 2316 2 -3.0762017055871524 2316 3 -2.608286339314269 2316 4 -2.5892631786431934 2316 5 -3.2709313605224395 2316 6 -2.7041334300376505 2316 7 -3.2851525004320976 2316 8 -3.081555947198556 2316 9 -2.6914351745838374 2316 10-11 -2.6584696769129437 2316 12-13 -2.6862690414369155 2316 14-15 -2.7263309642960465 2316 16-17 -3.8776666109020184 2316 18-19 -3.642544979496396 2316 20-21 -3.2720716039900033 2316 22-23 -3.2211824862407283 2316 24-25 -3.3913296887462216 2316 26-27 -3.005384169305266 2316 28-29 -3.21037495361702 2316 30-31 -3.2540762417290168 2316 32-33 -3.6910783431080567 2316 34-35 -3.5051884670253983 2316 36-37 -3.651684079836709 2316 38-39 -3.6125721578957126 2316 40-41 -3.394627122726604 2316 42-43 -4.034954151673528 2316 44-45 -3.641203899672849 2316 46-47 -3.3877627078834003 2316 48-49 -3.614262799098455 2316 50-51 -3.9347492797088606 2316 52-53 -4.738594994478575 2316 54-55 -3.717730233268128 2316 56-57 -3.685554143468636 2316 58-59 -3.9123628085244704 2316 60-61 -3.581738680041564 2316 62-63 -3.7312307168074845 2316 64-65 -3.685552236694999 2316 66-67 -3.4614473619460924 2316 68-69 -3.2784576592288417 2316 70-71 -3.2205266478724397 2316 72-73 -3.281702303439886 2316 74-75 -3.641326136558039 2316 76-77 -3.7320687984136214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30682.0 3 3126.0 4 4335.0 5 4988.0 6 5783.0 7 5960.0 8 7136.0 9 7725.0 10 9926.0 11 11262.0 12 8259.0 13 6295.0 14 6324.0 15 6333.0 16 6365.0 17 6531.0 18 6947.0 19 7260.0 20 7553.0 21 8122.0 22 8968.0 23 10098.0 24 11580.0 25 13280.0 26 15578.0 27 17807.0 28 20157.0 29 23609.0 30 27988.0 31 32633.0 32 39345.0 33 49254.0 34 68370.0 35 88083.0 36 108546.0 37 126945.0 38 100899.0 39 24327.0 40 163.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.962439453335048 26.19348362894094 30.380033781717806 21.464043136006207 2 21.777336336260344 26.71582272608413 30.37036802726253 21.136472910392996 3 21.7781850159322 26.55099041115823 29.99502316547764 21.675801407431933 4 21.570149115241133 26.793611094123328 30.315143284331196 21.32109650630434 5 21.689541770392793 26.802620382935245 30.082211193248497 21.425626653423464 6 21.80301613292663 26.692871294018662 30.207932452793184 21.296180120261525 7 21.903634174270813 26.665724715463956 29.799891605316827 21.630749504948408 8 21.623274534014786 26.988075291527437 29.55248337405245 21.83616680040533 9 21.557047234629447 26.86625079610111 29.93962284841813 21.63707912085131 10-11 22.10247604124433 26.828744918139268 29.4654865800916 21.6032924605248 12-13 22.189499789641502 26.74700357767606 29.152313258068226 21.91118337461421 14-15 21.797397525524715 26.77423589280753 29.39373826696055 22.034628314707202 16-17 21.97412792858427 26.71885222006496 29.461624956706796 21.84539489464398 18-19 22.14188516980004 26.97340845908038 28.926400211732712 21.95830615938686 20-21 21.92152355242484 27.088188461579023 28.83176387468233 22.15852411131381 22-23 22.214788171740274 27.08767645325142 28.39570027173827 22.301835103270033 24-25 22.18038373775249 27.21335658761056 27.9446220617574 22.661637612879552 26-27 22.330440064410475 27.443416749886374 27.69144135831436 22.534701827388794 28-29 22.474477894020986 27.36423135825314 27.42981256111994 22.731478186605937 30-31 22.178290687235062 27.163658850192235 27.683404054318355 22.974646408254348 32-33 22.130292373120376 27.55937848767725 27.556057204101307 22.754271935101062 34-35 22.112653854811985 27.08440419498354 27.663473456074506 23.139468494129964 36-37 22.03421908905145 27.05067807654431 28.08835344753052 22.826749386873722 38-39 22.08046225465128 27.04869806357056 28.07891226756784 22.791927414210324 40-41 22.08726091815862 27.015140971299633 27.920225216676158 22.977372893865585 42-43 21.97046142555729 27.100487459233413 27.86351985001903 23.065531265190266 44-45 21.937544383892266 27.01519345973198 28.0007465192748 23.046515637100953 46-47 21.820952340780032 26.99534427577916 28.011536494445295 23.172166888995513 48-49 21.849531714400356 27.029435320803184 27.75104278764251 23.369990177153948 50-51 21.743825817797358 27.145783732905155 27.76991201773883 23.340478431558658 52-53 21.654494159833366 27.013684182772167 27.941097371265855 23.390724286128616 54-55 21.60980777068942 26.890906953041256 27.979364494338892 23.519920781930434 56-57 21.60326932263102 26.98525093031863 27.969400979193974 23.44207876785637 58-59 21.570074734081114 26.829297868694724 27.802976182630584 23.797651214593575 60-61 21.55815287537645 26.559112458980437 27.86346074354021 24.0192739221029 62-63 21.521549653656393 26.390976769642617 27.879697995335682 24.207775581365308 64-65 21.47959191743863 26.19856720818612 27.8784357292111 24.443405145164157 66-67 21.463093377540712 26.384907904810717 27.89109082127438 24.260907896374192 68-69 21.474804513228975 26.013293311367658 28.113180881425436 24.398721293977932 70-71 21.187142845841255 25.832711431306414 28.314166444715767 24.66597927813656 72-73 21.342232238854475 25.459848885315537 28.342760855366933 24.85515802046305 74-75 21.27513315719724 25.40385037435954 28.317112493563716 25.003903974879506 76-77 21.258975098964168 25.19712374754865 28.55106329433154 24.992837859155642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24262.0 1 13939.5 2 3187.5 3 2598.5 4 2439.0 5 2361.5 6 2220.0 7 2156.0 8 2112.0 9 2068.0 10 2062.5 11 2083.0 12 2092.5 13 2076.0 14 2252.0 15 2544.5 16 2732.5 17 2804.0 18 2994.5 19 3350.0 20 3754.5 21 4228.5 22 4463.0 23 4930.5 24 5892.5 25 6900.0 26 7413.0 27 7891.5 28 9451.5 29 10986.5 30 11440.0 31 12700.0 32 14790.5 33 16432.5 34 18696.5 35 20149.0 36 21292.5 37 23552.5 38 25817.0 39 26965.0 40 28023.0 41 30371.5 42 33505.0 43 35348.0 44 36392.0 45 38438.0 46 40550.0 47 42644.5 48 43629.0 49 44350.5 50 45119.0 51 44238.0 52 43310.0 53 43227.5 54 41155.5 55 37511.0 56 34208.0 57 32560.0 58 31790.0 59 28549.0 60 23681.5 61 21285.0 62 19277.5 63 14697.0 64 10551.0 65 8978.0 66 8019.0 67 6296.5 68 4896.5 69 3663.0 70 3066.0 71 2596.5 72 1847.0 73 1474.5 74 1382.0 75 1159.0 76 747.0 77 439.5 78 321.0 79 260.5 80 182.5 81 132.0 82 79.0 83 59.0 84 56.5 85 44.5 86 37.0 87 39.0 88 31.0 89 18.0 90 14.5 91 16.0 92 12.5 93 14.0 94 15.5 95 11.5 96 11.0 97 15.0 98 16.5 99 28.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010542495746102967 2 0.014126944299777976 3 0.015602893704232389 4 0.015602893704232389 5 0.013810669427394886 6 0.011491320363252233 7 0.016446293363920627 8 0.01844936755568019 9 0.017922242768375042 10-11 0.02039972926870924 12-13 0.016973418151225775 14-15 0.010384358309911421 16-17 0.007116184628619501 18-19 0.018607504991871738 20-21 0.02445859013095888 22-23 0.02140126636458902 24-25 0.019609042087751518 26-27 0.026250814407796388 28-29 0.010489783267372453 30-31 0.010173508394989362 32-33 0.011965732671826868 34-35 0.013019982246437162 36-37 0.013072694725167679 38-39 0.01549746874677136 40-41 0.01286184481024562 42-43 0.0163408684064596 44-45 0.014812206523274668 46-47 0.026039964492874323 48-49 0.025565552184299695 50-51 0.03579177305801957 52-53 0.03869095938819789 54-55 0.028833725865591613 56-57 0.02224466602427726 58-59 0.018923779864254826 60-61 0.022560940896660348 62-63 0.029360850652896765 64-65 0.02672522671637102 66-67 0.029940687918932427 68-69 0.041748283154567745 70-71 0.053714015826394616 72-73 0.08275859160690828 74-75 0.08328571639421344 76-77 0.08966392632060573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 948542.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.62344178132998 #Duplication Level Percentage of deduplicated Percentage of total 1 74.99367526850061 39.464253044599076 2 11.654681124226872 12.266188672414367 3 5.001609372034038 7.896056988065629 4 2.6807875017248444 5.6428906010053765 5 1.6298834145725645 4.288503748855734 6 1.0207546523048594 3.2229373811151896 7 0.692195578795214 2.5497999619416767 8 0.4732104086035619 1.992156830997513 9 0.33585049316630144 1.5906247986931046 >10 1.4237187030519192 12.543905343671845 >50 0.06316045767475229 2.282224979336834 >100 0.029270142814087078 2.6341165296225344 >500 8.019216869007262E-4 0.2284068461906016 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 4.009608434503631E-4 3.3979342734904834 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18488 1.9490966135395165 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13720 1.446430416365327 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.16274872383089E-4 0.0 2 0.0 0.0 0.0 0.003689873511136038 0.0 3 0.0 0.0 0.0 0.006009222575278691 0.0 4 0.0 0.0 0.0 0.011702170278174292 0.0 5 0.0 0.0 0.0 0.020768716619822845 0.0 6 0.0 0.0 0.0 0.03247088689799714 0.0 7 0.0 0.0 0.0 0.05619150232672881 0.0 8 0.0 0.0 0.0 0.0821260418621421 0.0 9 0.0 0.0 0.0 0.11196130482361351 0.0 10 0.0 0.0 0.0 0.14622441599844815 0.0 11 0.0 0.0 0.0 0.18860524889778207 0.0 12 0.0 0.0 0.0 0.245218451054355 0.0 13 0.0 0.0 0.0 0.30467812706237574 0.0 14 0.0 0.0 0.0 0.3781593224127134 0.0 15 0.0 0.0 0.0 0.4474235194646099 0.0 16 0.0 0.0 0.0 0.518796215665727 0.0 17 0.0 0.0 0.0 0.586268188440786 0.0 18 0.0 0.0 0.0 0.6436193652995861 0.0 19 0.0 0.0 0.0 0.714254086798476 0.0 20 0.0 0.0 0.0 0.7898437812980342 0.0 21 0.0 0.0 0.0 0.8773464959906888 0.0 22 0.0 0.0 0.0 0.9707530083011612 0.0 23 0.0 0.0 0.0 1.0631052710370232 0.0 24 0.0 0.0 0.0 1.1555629587303462 0.0 25 0.0 0.0 0.0 1.2466501219766757 0.0 26 0.0 0.0 0.0 1.3419542835214466 0.0 27 0.0 0.0 0.0 1.432092622150627 0.0 28 0.0 0.0 0.0 1.521387561120119 0.0 29 0.0 0.0 0.0 1.6117367496642214 0.0 30 0.0 0.0 0.0 1.713577258571576 0.0 31 0.0 0.0 0.0 1.8321803357152346 0.0 32 0.0 0.0 0.0 1.9632235578392945 0.0 33 0.0 0.0 0.0 2.093528805261127 0.0 34 0.0 0.0 0.0 2.228472750811245 0.0 35 0.0 0.0 0.0 2.3683716693620314 0.0 36 0.0 0.0 0.0 2.512909286041103 0.0 37 0.0 0.0 0.0 2.668305673338661 0.0 38 0.0 0.0 0.0 2.8055689679529214 0.0 39 0.0 0.0 0.0 2.9591731309736415 0.0 40 0.0 0.0 0.0 3.121632990421088 0.0 41 0.0 0.0 0.0 3.2890478228692035 0.0 42 0.0 0.0 0.0 3.472698098766317 0.0 43 0.0 0.0 0.0 3.6763791165810265 0.0 44 0.0 0.0 0.0 3.9000908763133313 0.0 45 0.0 0.0 0.0 4.118953088002429 0.0 46 0.0 0.0 0.0 4.347514395777941 0.0 47 0.0 0.0 0.0 4.583771725448109 0.0 48 0.0 0.0 0.0 4.8184476807563605 0.0 49 1.0542495746102966E-4 0.0 0.0 5.066512605662163 0.0 50 1.0542495746102966E-4 0.0 0.0 5.31700230458957 0.0 51 1.0542495746102966E-4 0.0 0.0 5.5766639748160864 0.0 52 1.0542495746102966E-4 0.0 0.0 5.856778086790042 0.0 53 1.0542495746102966E-4 0.0 0.0 6.1483835191272505 0.0 54 1.0542495746102966E-4 0.0 0.0 6.470140489298313 0.0 55 1.0542495746102966E-4 0.0 0.0 6.786099086809019 0.0 56 1.0542495746102966E-4 0.0 0.0 7.125778299748456 0.0 57 1.0542495746102966E-4 0.0 0.0 7.482220080924197 0.0 58 1.0542495746102966E-4 0.0 0.0 7.843827685015529 0.0 59 1.0542495746102966E-4 0.0 0.0 8.204064764659867 0.0 60 1.0542495746102966E-4 0.0 0.0 8.57800708877414 0.0 61 1.0542495746102966E-4 0.0 0.0 8.96407328299643 0.0 62 1.0542495746102966E-4 0.0 0.0 9.339175281642774 0.0 63 1.0542495746102966E-4 0.0 0.0 9.740422669739452 0.0 64 1.0542495746102966E-4 0.0 0.0 10.154004777859072 0.0 65 1.0542495746102966E-4 0.0 0.0 10.590147826875352 1.0542495746102966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 155 3.6379788E-11 27.479076 4 GCCGTAT 95 2.5159318E-4 22.434881 71 AATCCGC 145 4.025569E-7 22.030638 6 CGTATTA 130 3.8450416E-6 21.843494 41 ACGGTTA 85 0.0034915498 20.882011 19 GACGTAT 145 9.887239E-6 19.584854 39 ACGTATT 145 9.891741E-6 19.58382 40 TAAGGCG 365 0.0 19.451736 17 CCGATAT 95 0.0066457475 18.68292 39 ACCGCGA 95 0.006649794 18.68095 5 TGATCCG 155 1.7562583E-5 18.32228 34 GAGACGT 160 2.3067798E-5 17.750647 37 CCCGATA 100 0.008932784 17.748774 38 AAATCCG 165 3.0101599E-5 17.209118 5 TCGCGCT 190 5.3452277E-6 16.816402 37 CGATCGT 190 5.3532804E-6 16.813744 1 ATTAAGG 445 0.0 16.752535 15 TATTAAG 445 0.0 16.752535 14 CGCGATC 170 3.8833467E-5 16.70561 35 GATGTAT 385 0.0 16.597132 54 >>END_MODULE