FastQCFastQC Report
Thu 26 May 2016
SRR936081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948542
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82310.8677528248617351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64240.6772499267296546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51740.5454687299033675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48650.5128924180479093No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33810.35644178117574127No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.19292767215368428No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17410.18354485093965264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.15128481395657756No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.14727866557305844No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT11760.12397974997417088RNA PCR Primer, Index 23 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.10310560839688701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA6650.051.46433100-101
GCGTACA1000.047.615692100-101
ACGTATC1750.045.915134100-101
CCGTACA2500.045.23491100-101
TACGCAG4550.044.4762100-101
ACCGTAC1150.043.99160498-99
AGTATCA14200.043.801407100-101
CCGGTAT2550.043.1809100-101
CGTACAT5500.042.745907100-101
TCGGTAT1200.042.15973100-101
AGGTATC10500.041.380306100-101
GGTATCA54400.040.2526551
CGCGTAC300.001306777239.678798-99
CGGTATC6650.039.3814100-101
TTATACG2750.038.9531696-97
CCCGTAC1300.038.9156598-99
TCCGGTA1300.038.9156598-99
ATACACG3950.038.41899596-97
CCCGTAT1800.038.02541798-99
TTTACGC1500.037.69476798-99
Produced by FastQC (version 0.11.5)