FastQCFastQC Report
Thu 26 May 2016
SRR936080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947380
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182401.9253098017690893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134121.415693808186789No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG450.004019455531.55508624
CGGTTAC1809.094947E-1225.6358014
ACGGTTA800.00245919222.1824861
GCCTAGT2657.8216544E-1118.75245917
CGATCGT1752.4462734E-618.2530161
CCACTCG1000.00892183817.75254849
TAGTGTA1000.00892455317.75161254
TCGCGCT1803.2011922E-617.74598737
GAGACGT1452.1921782E-417.13405837
CGCGGAA1905.3343974E-616.8199859
CGCCGTA1300.002075498516.39389471
AGGCAAT2301.9092167E-615.43618455
TTGCGCG2553.3243305E-715.311885
TAAGCCG1655.8636436E-415.057229
GGCAGCG1901.0065084E-414.943992
CAATTCG2151.7255446E-514.86181354
CGCGCTT2402.9799303E-614.789103538
TCGCGGA2252.649047E-514.20203858
CCCTACC1759.1394526E-414.20053912
AGTCACG2252.6556505E-514.1982894
Produced by FastQC (version 0.11.5)