##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936080_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 947380 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12270261141253 39.0 35.0 41.0 29.0 41.0 2 35.968258776837175 39.0 35.0 41.0 28.0 41.0 3 35.80082015664253 39.0 35.0 41.0 27.0 41.0 4 35.68268698938124 39.0 35.0 40.0 27.0 41.0 5 35.5777861048365 39.0 35.0 40.0 27.0 41.0 6 35.43431041398383 38.0 35.0 40.0 27.0 41.0 7 35.215902805632375 38.0 35.0 40.0 27.0 41.0 8 35.024259536827884 38.0 34.0 40.0 26.0 41.0 9 35.10074310202875 38.0 34.0 40.0 26.0 41.0 10-11 35.246217990669 38.0 35.0 40.0 26.5 41.0 12-13 35.06846038548418 38.0 35.0 41.0 25.5 41.0 14-15 34.83506143258249 38.0 34.0 41.0 24.5 41.0 16-17 34.59053442124596 38.0 34.0 40.5 24.0 41.0 18-19 34.31877388165256 38.0 33.5 40.0 22.5 41.0 20-21 33.970264835652 38.0 33.0 40.0 20.5 41.0 22-23 33.51104678165045 38.0 33.0 40.0 16.0 41.0 24-25 33.136865354978994 38.0 33.0 40.0 8.5 41.0 26-27 32.10625725685575 36.5 31.5 39.5 7.0 40.5 28-29 32.060139014967596 37.0 31.5 39.5 3.5 40.5 30-31 32.390283202094196 37.0 32.0 40.0 2.0 41.0 32-33 32.21226962781566 37.0 32.0 40.0 2.0 41.0 34-35 31.917908864447213 36.0 31.0 40.0 2.0 41.0 36-37 31.565344951339483 36.0 31.0 39.5 2.0 41.0 38-39 31.2199333952585 35.0 31.0 39.0 2.0 41.0 40-41 30.82922111507526 35.0 30.0 39.0 2.0 41.0 42-43 30.4241165107982 35.0 30.0 38.5 2.0 40.0 44-45 30.05051774367202 35.0 29.0 38.0 2.0 40.0 46-47 29.62309052333805 35.0 29.0 37.0 2.0 40.0 48-49 29.208509784880405 35.0 29.0 36.5 2.0 39.0 50-51 28.767737338765862 34.0 28.5 36.0 2.0 39.0 52-53 28.271176824505478 34.0 27.0 35.5 2.0 38.5 54-55 27.806592919419874 34.0 26.0 35.0 2.0 37.0 56-57 27.37096624374591 34.0 26.0 35.0 2.0 37.0 58-59 26.93902288416475 34.0 25.0 35.0 2.0 36.0 60-61 26.576351094597733 33.0 24.5 35.0 2.0 36.0 62-63 26.173132745044228 33.0 23.5 35.0 2.0 35.5 64-65 25.770605775929404 33.0 21.0 35.0 2.0 35.0 66-67 25.378000379995356 33.0 19.5 35.0 2.0 35.0 68-69 24.977538580084023 32.5 16.5 35.0 2.0 35.0 70-71 24.49283919863202 32.0 4.5 35.0 2.0 35.0 72-73 23.97438250754713 32.0 2.0 35.0 2.0 35.0 74-75 23.367823893263527 32.0 2.0 34.5 2.0 35.0 76-77 21.726790200341995 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0530808983428912 1101 2 0.6582836201376168 1101 3 0.6744034864458612 1101 4 0.6978975879802363 1101 5 0.8905974187931562 1101 6 0.8272336985167286 1101 7 0.953581437119837 1101 8 0.8813644189222174 1101 9 0.7869518408055427 1101 10-11 0.9211400966060381 1101 12-13 1.1194514606001391 1101 14-15 1.0899105513469252 1101 16-17 1.161243502746082 1101 18-19 1.273512640293049 1101 20-21 1.3551217096455233 1101 22-23 1.4955430916645085 1101 24-25 1.3623635175638071 1101 26-27 1.229537863616052 1101 28-29 1.2716048331280732 1101 30-31 1.5152986971340638 1101 32-33 1.4126540528103178 1101 34-35 1.5096969640110416 1101 36-37 1.4326582651895556 1101 38-39 1.3759567483620003 1101 40-41 1.3279564872786978 1101 42-43 1.4990428300741279 1101 44-45 1.4118600315701002 1101 46-47 1.2386561645767529 1101 48-49 1.3369349022228327 1101 50-51 1.350597846823817 1101 52-53 1.1459415282415648 1101 54-55 1.4197288439721376 1101 56-57 1.246032848868051 1101 58-59 1.2512470036632592 1101 60-61 1.1331267089217256 1101 62-63 1.0658998364694838 1101 64-65 1.0556715001130357 1101 66-67 1.13827639459522 1101 68-69 0.9686367748335272 1101 70-71 0.840929298229895 1101 72-73 0.9955820579415615 1101 74-75 0.6325962777060283 1101 76-77 0.8400175375535 1102 1 0.5697798943376498 1102 2 0.46853740536311506 1102 3 0.7758277729555516 1102 4 0.7413446127840118 1102 5 0.5702856203844817 1102 6 0.713025856858593 1102 7 0.7698385989640499 1102 8 0.991993583568302 1102 9 1.0314064630014244 1102 10-11 1.167024842551406 1102 12-13 1.3444501135448022 1102 14-15 1.2902423759782877 1102 16-17 1.2395590549489626 1102 18-19 0.9980173729474728 1102 20-21 1.0057382119714404 1102 22-23 1.0644674230760387 1102 24-25 1.1528492657183378 1102 26-27 1.0654889744876783 1102 28-29 1.211930281697498 1102 30-31 0.8975413646628496 1102 32-33 0.9266926145144296 1102 34-35 0.998089837190264 1102 36-37 1.0618925091322957 1102 38-39 1.1495526676823715 1102 40-41 1.0903853178550662 1102 42-43 1.0874815031348213 1102 44-45 0.8222314596283624 1102 46-47 0.954665468238943 1102 48-49 0.8094796693618633 1102 50-51 0.8410988616055057 1102 52-53 0.9485485293371667 1102 54-55 0.7838850485090205 1102 56-57 0.9346610077128155 1102 58-59 1.047563256334918 1102 60-61 0.8572363053439496 1102 62-63 0.6516533792250243 1102 64-65 0.6568084148174904 1102 66-67 0.7528389608254429 1102 68-69 0.8361269413295673 1102 70-71 0.49398397071583844 1102 72-73 0.7192763123015276 1102 74-75 0.8787908463789087 1102 76-77 0.8949437189210308 1103 1 0.8053487951357781 1103 2 0.6394517650930354 1103 3 0.8524571322977508 1103 4 0.760858440044494 1103 5 0.5312723258806287 1103 6 0.730008013101326 1103 7 0.8811660676663919 1103 8 0.68078616460906 1103 9 0.77076784432019 1103 10-11 0.9807718305378117 1103 12-13 0.7744550821107197 1103 14-15 0.7804955143600267 1103 16-17 0.826086056089153 1103 18-19 0.5083379172495626 1103 20-21 0.6565456757339447 1103 22-23 0.4220828273535986 1103 24-25 0.8726120956756347 1103 26-27 0.5708028988218743 1103 28-29 0.5756336152318724 1103 30-31 0.8224844271949792 1103 32-33 0.9990434067565701 1103 34-35 1.1229760340546164 1103 36-37 1.0968447907954193 1103 38-39 1.0959991378465617 1103 40-41 1.3150140396124996 1103 42-43 1.0404883471189805 1103 44-45 0.9369256343438188 1103 46-47 0.941680422760772 1103 48-49 1.295844115108288 1103 50-51 1.365607820820184 1103 52-53 1.4120008497723333 1103 54-55 1.2880824815123617 1103 56-57 1.2478228250017018 1103 58-59 1.0180692171780237 1103 60-61 0.9624377907441222 1103 62-63 0.9373035577200355 1103 64-65 0.6659880557596232 1103 66-67 0.8340843476699433 1103 68-69 0.8980351291241284 1103 70-71 0.7178587915746242 1103 72-73 0.7843099578216375 1103 74-75 0.8003449448713944 1103 76-77 0.8248086773200605 1104 1 0.7430928202999283 1104 2 0.7036214471316811 1104 3 0.8192724595526784 1104 4 0.9011612237185957 1104 5 0.6867032949423617 1104 6 0.6066316396864622 1104 7 0.6768200826021058 1104 8 0.49408423000505053 1104 9 0.5515906284342194 1104 10-11 0.763887763800021 1104 12-13 0.8976486085011857 1104 14-15 1.1493254583785912 1104 16-17 1.0831002329200956 1104 18-19 1.111414146586469 1104 20-21 1.1540160627647253 1104 22-23 1.1480295075115379 1104 24-25 1.5445487893614995 1104 26-27 1.2120102940903976 1104 28-29 1.2241892612180365 1104 30-31 1.4000569336014976 1104 32-33 1.6388072728850531 1104 34-35 1.5639590636510832 1104 36-37 1.5261338595678957 1104 38-39 1.4774853954491505 1104 40-41 1.315753676448061 1104 42-43 1.476130318897301 1104 44-45 1.2987304032141793 1104 46-47 1.2763220975845222 1104 48-49 1.056141289640582 1104 50-51 1.120793527407077 1104 52-53 1.2783383001424262 1104 54-55 1.2235897239042757 1104 56-57 1.0242586553196666 1104 58-59 1.0011524304463961 1104 60-61 1.083845465369901 1104 62-63 0.9569314296556293 1104 64-65 0.6946826654843328 1104 66-67 0.7907083591961808 1104 68-69 0.7733430820073366 1104 70-71 0.5449982392391348 1104 72-73 0.2668295073305593 1104 74-75 0.35463126406455814 1104 76-77 0.8887542686445897 1105 1 -0.4681145529719615 1105 2 -0.30028638696970233 1105 3 -0.2974003515072283 1105 4 -0.09057149517541774 1105 5 -0.45330592164749817 1105 6 -0.5716817243688368 1105 7 -0.4146730049554961 1105 8 -0.3371231432668367 1105 9 -0.6519024591233844 1105 10-11 -0.48897859564697654 1105 12-13 -0.26124079242047316 1105 14-15 -0.3571676291790098 1105 16-17 -0.4424942371259917 1105 18-19 -0.3436088948881846 1105 20-21 -0.4101682690325106 1105 22-23 -0.5410027505529769 1105 24-25 -0.34925766225140364 1105 26-27 -0.6377869409326351 1105 28-29 -0.5818706466160641 1105 30-31 -0.40784629220495816 1105 32-33 -0.2004553999260068 1105 34-35 -0.31867688104937386 1105 36-37 -0.23724862891137022 1105 38-39 -0.559284189804309 1105 40-41 -0.6234905631832746 1105 42-43 -0.5542706027616866 1105 44-45 -0.6363801958641595 1105 46-47 -0.590687119005338 1105 48-49 -0.6937481112810744 1105 50-51 -0.8882571638371637 1105 52-53 -0.7895879671386794 1105 54-55 -0.9170508290911172 1105 56-57 -0.7839671511319501 1105 58-59 -0.638151717019042 1105 60-61 -0.6052144424641988 1105 62-63 -0.8856538238005918 1105 64-65 -0.7802574266815618 1105 66-67 -1.0312132997899965 1105 68-69 -1.3365785769788374 1105 70-71 -1.2295731893322959 1105 72-73 -1.721552981148701 1105 74-75 -1.6305899340921215 1105 76-77 -1.397794118452186 1106 1 -0.007788483561149917 1106 2 0.3384674467671829 1106 3 0.15898060087372556 1106 4 0.1570088115235322 1106 5 0.031233222823850326 1106 6 0.059188332128336185 1106 7 -0.3809619476512225 1106 8 0.06983730871056792 1106 9 0.1908223189234235 1106 10-11 0.09175102501484389 1106 12-13 0.20523862646572155 1106 14-15 0.1570599737265539 1106 16-17 0.1151877644882191 1106 18-19 0.3042361414314314 1106 20-21 0.2765758794743505 1106 22-23 0.38800451336953756 1106 24-25 0.002680997958442788 1106 26-27 0.019962857291737635 1106 28-29 0.17921087073663955 1106 30-31 0.4530738046473459 1106 32-33 0.3550248260626958 1106 34-35 0.1651358711701505 1106 36-37 0.431732807731084 1106 38-39 0.26926141148705796 1106 40-41 0.1779396224502996 1106 42-43 0.3131418266168424 1106 44-45 0.14412666450710532 1106 46-47 0.2640925420116105 1106 48-49 0.1285214609545946 1106 50-51 0.09107132688115982 1106 52-53 0.1373531143786728 1106 54-55 0.16022762127207812 1106 56-57 0.07456392231437903 1106 58-59 0.16787572446602184 1106 60-61 0.11979927827833237 1106 62-63 0.100118573293841 1106 64-65 0.21288543046129504 1106 66-67 0.24008936364825928 1106 68-69 0.3450808257653115 1106 70-71 -0.20927940402156153 1106 72-73 -0.08501706508736007 1106 74-75 -0.1377279486199683 1106 76-77 -0.09452373911400613 1107 1 0.02429450245348619 1107 2 0.17860822661343434 1107 3 0.3043357921305514 1107 4 0.1853129700861018 1107 5 0.2650203859247142 1107 6 0.022066909510940036 1107 7 -0.027419296914906965 1107 8 -0.21183352578049863 1107 9 -0.1314153396854465 1107 10-11 -0.2006913200810132 1107 12-13 0.02218777900809954 1107 14-15 -0.35348925291047095 1107 16-17 -0.23627097405026376 1107 18-19 -0.22626307131285728 1107 20-21 -0.08713626278738928 1107 22-23 -0.06921508466695059 1107 24-25 0.15605693571892942 1107 26-27 -0.08813198386784649 1107 28-29 0.009635988824996389 1107 30-31 0.23440195135476216 1107 32-33 0.2994353104565093 1107 34-35 0.18700305649941384 1107 36-37 0.2640566013774652 1107 38-39 0.24186491245954755 1107 40-41 0.21482949926793538 1107 42-43 0.0546255721017026 1107 44-45 0.1548703896159367 1107 46-47 0.015782826349852286 1107 48-49 0.19404892228644854 1107 50-51 0.12848912812224356 1107 52-53 0.05890539742026135 1107 54-55 0.007064705647369607 1107 56-57 -0.022546386104625782 1107 58-59 -0.10335702537189206 1107 60-61 -0.03521600374444844 1107 62-63 -0.02211908531503326 1107 64-65 0.2210821517727659 1107 66-67 0.19491940903123606 1107 68-69 0.10175397571437017 1107 70-71 0.33965085438354947 1107 72-73 0.05859690175527632 1107 74-75 -0.13033700669165427 1107 76-77 0.24054155680152078 1108 1 0.13475932628685428 1108 2 0.19616430041822497 1108 3 0.3548662354622891 1108 4 0.40543209119637424 1108 5 0.3576970669956623 1108 6 0.0063433802336732015 1108 7 0.18444594304211392 1108 8 0.2899647228419511 1108 9 0.3909558791910186 1108 10-11 0.3883334186985081 1108 12-13 0.4979199965813166 1108 14-15 0.19266560453288406 1108 16-17 0.26476876347174283 1108 18-19 0.1618065265105031 1108 20-21 0.06470142612588603 1108 22-23 0.18785558776142608 1108 24-25 0.15369451945809942 1108 26-27 0.09001580861906433 1108 28-29 0.09371811608566816 1108 30-31 0.25136470600185845 1108 32-33 0.13182493480202595 1108 34-35 0.18986047699007713 1108 36-37 0.3421901039039348 1108 38-39 0.3861437838956334 1108 40-41 0.3140641289864803 1108 42-43 0.31842395790776834 1108 44-45 0.25061083820637364 1108 46-47 0.378128780576251 1108 48-49 0.30838518220367916 1108 50-51 0.25940631494347244 1108 52-53 0.5892584166031014 1108 54-55 0.47062759290529854 1108 56-57 0.5561792923826943 1108 58-59 0.5012391975057611 1108 60-61 0.6416588390659861 1108 62-63 0.5169188002280976 1108 64-65 0.18453815012656705 1108 66-67 0.46652011324048814 1108 68-69 0.6549229173427378 1108 70-71 0.39623852614887767 1108 72-73 0.22432328902463894 1108 74-75 0.49815603123961694 1108 76-77 0.6526177165747136 1109 1 0.33247098494690874 1109 2 0.5137900386622789 1109 3 0.17050323461857886 1109 4 0.42913338430724224 1109 5 0.3140050718857168 1109 6 0.3245158064953557 1109 7 0.3271917804648439 1109 8 0.15048179500477232 1109 9 0.09673010995362574 1109 10-11 0.06402904691621103 1109 12-13 0.007887367485459151 1109 14-15 0.31174889051528254 1109 16-17 0.33036702095748893 1109 18-19 0.14468916507654228 1109 20-21 -0.06119178461158015 1109 22-23 -0.08579404978872418 1109 24-25 -0.04383849705445897 1109 26-27 -0.09781457173804853 1109 28-29 0.1175679189336094 1109 30-31 0.14767692942938027 1109 32-33 0.010882048633668262 1109 34-35 0.07580930354792059 1109 36-37 0.11236160036552789 1109 38-39 0.2040385615184448 1109 40-41 0.24443889007951114 1109 42-43 0.2605977099955119 1109 44-45 0.20884731736335382 1109 46-47 0.38334168757902276 1109 48-49 0.33245882581474717 1109 50-51 0.38334307131862744 1109 52-53 0.5346154426202858 1109 54-55 0.5335117811381593 1109 56-57 0.5258682398145531 1109 58-59 0.37634681325726405 1109 60-61 0.3784586909867116 1109 62-63 0.4109646359289769 1109 64-65 0.4210130024777534 1109 66-67 0.07372262025703691 1109 68-69 0.1757723930387982 1109 70-71 0.3499659053179087 1109 72-73 -0.029070911777747455 1109 74-75 0.14253117114009228 1109 76-77 0.23062622252371412 1110 1 -0.15020602671560823 1110 2 -0.04092783248071186 1110 3 -0.20906946500242896 1110 4 -0.1650898463894208 1110 5 -0.26151258454981274 1110 6 -0.06833785085105148 1110 7 -0.01306261534510611 1110 8 -0.3642061472194058 1110 9 0.029833283394964383 1110 10-11 -0.21298424217436462 1110 12-13 -0.07553949371917668 1110 14-15 0.19822028725238283 1110 16-17 0.10584116660071885 1110 18-19 0.15342385497065436 1110 20-21 -0.11937831985125769 1110 22-23 -0.015164241236206522 1110 24-25 0.11193827224887798 1110 26-27 -0.04857011428102709 1110 28-29 0.20378435056067445 1110 30-31 0.28397753614061827 1110 32-33 0.4401770111411878 1110 34-35 0.22987817259263466 1110 36-37 0.09082985781929054 1110 38-39 -0.01185223045930428 1110 40-41 0.06444450175178673 1110 42-43 0.023982361665186858 1110 44-45 -0.010337282256028146 1110 46-47 -0.14930767236502263 1110 48-49 -0.04275262850298844 1110 50-51 -0.05592684198510156 1110 52-53 -0.020322015698816642 1110 54-55 0.32900902409917165 1110 56-57 0.34682336270204317 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-0.17458539579799037 2314 32-33 -0.2834113204100177 2314 34-35 -0.3688801160166548 2314 36-37 -0.6970846580507661 2314 38-39 -0.507587931006924 2314 40-41 -0.17250253997053377 2314 42-43 -0.14371571944275985 2314 44-45 -0.10923264678402589 2314 46-47 0.073216078920332 2314 48-49 0.21038474462705992 2314 50-51 0.4015450339650357 2314 52-53 0.4433148603708901 2314 54-55 0.3918845950353216 2314 56-57 0.30286085232526716 2314 58-59 0.13499163401194636 2314 60-61 0.27047045690609295 2314 62-63 0.2609509422199032 2314 64-65 0.43224584532732635 2314 66-67 0.6078537455242383 2314 68-69 0.6621022478143459 2314 70-71 0.8789592220765172 2314 72-73 0.899472824506315 2314 74-75 1.0149213635989476 2314 76-77 1.0024814711267744 2315 1 -0.8381038975389288 2315 2 -0.7496701064592344 2315 3 -0.8443729695223468 2315 4 -0.715533953359035 2315 5 -0.685468478951428 2315 6 -0.725284499737441 2315 7 -1.3286425254610137 2315 8 -1.2676972976467127 2315 9 -0.7501177979909386 2315 10-11 -0.8274947646471063 2315 12-13 -0.7497983441488856 2315 14-15 -0.6739571224264438 2315 16-17 -0.5245841888048375 2315 18-19 -0.5589328943925835 2315 20-21 -0.4354360177625338 2315 22-23 -0.7999223392036967 2315 24-25 -0.8788787748768527 2315 26-27 -0.7178231198447946 2315 28-29 -0.6861303418211193 2315 30-31 -0.7549259601519225 2315 32-33 -0.7433174987985147 2315 34-35 -1.0881086738879304 2315 36-37 -1.2318277393066097 2315 38-39 -0.9340392387449583 2315 40-41 -0.9456181805788901 2315 42-43 -1.0759982697174557 2315 44-45 -0.9394935647156046 2315 46-47 -0.8909958787624959 2315 48-49 -0.7855969528124831 2315 50-51 -0.6838175652744063 2315 52-53 -0.6501851669899992 2315 54-55 -0.8411777030844263 2315 56-57 -1.0659792933695904 2315 58-59 -0.9259286963276807 2315 60-61 -0.9343449095686474 2315 62-63 -0.8871644172834294 2315 64-65 -0.8094909789055755 2315 66-67 -0.7182389471695032 2315 68-69 -0.7051247298719119 2315 70-71 -0.6574240132698499 2315 72-73 -0.7218191191737304 2315 74-75 -0.9602660584881093 2315 76-77 -0.9667783831034065 2316 1 -2.967575167124167 2316 2 -2.4060861199470054 2316 3 -2.603947748035935 2316 4 -3.696536782224811 2316 5 -3.080937430325733 2316 6 -2.8546349953968786 2316 7 -2.4499360223119737 2316 8 -2.1314035170986116 2316 9 -2.728703527214172 2316 10-11 -2.592538008197046 2316 12-13 -3.6030458658517155 2316 14-15 -4.551763089793166 2316 16-17 -3.752590365296882 2316 18-19 -3.438762432938919 2316 20-21 -3.083311126175367 2316 22-23 -3.3360094261203983 2316 24-25 -3.3718451121846478 2316 26-27 -3.154967181253067 2316 28-29 -3.6658759870700024 2316 30-31 -3.7809037020847924 2316 32-33 -3.9988666148792795 2316 34-35 -3.797509991863791 2316 36-37 -3.894541018764503 2316 38-39 -3.807297540939153 2316 40-41 -3.4501901626492284 2316 42-43 -3.442943499957952 2316 44-45 -3.6605884735677634 2316 46-47 -3.5777338479772993 2316 48-49 -3.281302183377207 2316 50-51 -3.6466576978825564 2316 52-53 -3.783806925750163 2316 54-55 -3.6124874112005934 2316 56-57 -3.7067708894497073 2316 58-59 -3.932293239048416 2316 60-61 -3.8251450165629954 2316 62-63 -3.7331144379527927 2316 64-65 -3.7216940087910437 2316 66-67 -3.6924229126070856 2316 68-69 -3.720936387392726 2316 70-71 -3.5714236566220166 2316 72-73 -3.4862552508416265 2316 74-75 -3.3884724441054743 2316 76-77 -3.5130384442198768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30601.0 3 3256.0 4 4439.0 5 5341.0 6 5877.0 7 6080.0 8 7264.0 9 7764.0 10 10015.0 11 11164.0 12 8512.0 13 6543.0 14 6171.0 15 6519.0 16 6333.0 17 6540.0 18 6936.0 19 7154.0 20 7833.0 21 8220.0 22 9103.0 23 10449.0 24 11493.0 25 13275.0 26 15331.0 27 17604.0 28 20517.0 29 23544.0 30 27646.0 31 32487.0 32 38933.0 33 48932.0 34 67852.0 35 86789.0 36 107527.0 37 128548.0 38 100645.0 39 23993.0 40 150.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.90604066518463 26.242471071815366 30.449909536747928 21.401578726252076 2 21.796552699695553 26.683007984866336 30.294565983878257 21.225873331559857 3 21.83300730631303 26.55756637808085 29.92368539676943 21.68574091883669 4 21.54282106561202 26.743449461239432 30.384174396678965 21.329555076469582 5 21.679959882815595 26.734619546570244 30.127477632030402 21.457942938583756 6 21.691986106858945 26.64548208988313 30.26297731279626 21.39955449046166 7 21.93966536596902 26.63706312606632 29.745646041318263 21.677625466646397 8 21.643036699737007 26.82107703661316 29.640942045434148 21.894944218215677 9 21.537556826699944 26.727711158831813 30.025085042093462 21.70964697237478 10-11 22.059699995512865 26.78496862965573 29.5118262370632 21.643505137768205 12-13 22.164359611346256 26.687268454243025 29.268914563112947 21.879457371297775 14-15 21.86905213065018 26.694011251006817 29.403838094443852 22.033098523899152 16-17 21.991012464742344 26.670410593342 29.49513993277822 21.843437009137435 18-19 22.116519469880433 26.88301392017803 28.99220698056918 22.008259629372358 20-21 21.91356754000652 26.984280304829706 28.903453350265973 22.1986988048978 22-23 22.19763015263388 27.113997174932486 28.40406911069493 22.284303561738707 24-25 22.149408977461327 27.169299768151927 28.000774938448348 22.68051631593839 26-27 22.309381416634555 27.42241975412908 27.7239257003204 22.54427312891596 28-29 22.533669447206687 27.26845195492865 27.516103697041476 22.681774900823186 30-31 22.22879060824017 27.128460564436658 27.693013342679208 22.949735484643963 32-33 22.155897857076127 27.494759999809983 27.584543332202028 22.76479881091187 34-35 22.15550340508773 27.0076082676438 27.73227807811802 23.104610249150443 36-37 22.090904580382336 27.01448042234653 28.0758361580907 22.818778839180432 38-39 22.089644891702314 26.94514592421485 28.09072807969561 22.87448110438723 40-41 22.037566342837085 27.03182608741551 27.9799843231645 22.9506232465829 42-43 22.037219343923947 27.0582149389399 27.90885985077424 22.995705866361916 44-45 21.951986858094234 26.879145776125185 28.096366233494756 23.072501132285826 46-47 21.78584870253644 26.999087322974173 28.013219828475837 23.201844146013546 48-49 21.881160994658693 27.019618586429306 27.73675506564074 23.36246535327127 50-51 21.687633002802727 27.13397074373874 27.77291211665761 23.405484136800922 52-53 21.681917938251225 26.939924261901982 28.012220726982267 23.365937072864526 54-55 21.583764941763878 26.844152710990137 28.0106172770422 23.561465070203788 56-57 21.609004402858663 26.92014731444624 28.05509353369393 23.41575474900116 58-59 21.638189368534018 26.765633833447794 27.85809791109002 23.738078886928175 60-61 21.588621797663485 26.517096284284026 27.89455430570446 23.99972761234803 62-63 21.54733290642802 26.324413898249073 27.877004814034873 24.251248381288033 64-65 21.437609569071867 26.23956569868446 27.870518324726174 24.4523064075175 66-67 21.419043149642544 26.288072864675737 27.992605422092197 24.300278563589515 68-69 21.436445187775583 25.951178771138476 28.22572981602554 24.386646225060396 70-71 21.311097111674492 25.75265600585512 28.37895852992538 24.557288352545008 72-73 21.379245514193613 25.430360681225732 28.401111575852063 24.789282228728595 74-75 21.294180655424956 25.364996466498287 28.28787316999999 25.05294970807677 76-77 21.295306986572754 25.061780753981356 28.596395141199192 25.046517118246697 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23633.0 1 13556.5 2 3025.0 3 2412.5 4 2255.0 5 2321.0 6 2243.0 7 2165.0 8 2132.5 9 2034.0 10 2036.5 11 2064.5 12 2135.0 13 2180.0 14 2268.0 15 2412.0 16 2639.5 17 2811.0 18 3032.5 19 3405.0 20 3769.5 21 4278.0 22 4573.0 23 4969.0 24 5804.5 25 6885.5 26 7527.0 27 8012.5 28 9663.5 29 11151.5 30 11474.0 31 12640.5 32 14702.5 33 16540.5 34 18813.5 35 20144.0 36 21375.0 37 23690.0 38 25865.0 39 26956.0 40 28009.0 41 30616.5 42 33755.0 43 35339.0 44 36349.5 45 38314.5 46 40414.0 47 42609.5 48 43660.0 49 44531.0 50 45294.0 51 44250.5 52 43315.0 53 43189.5 54 41165.5 55 37379.0 56 34064.5 57 32638.0 58 31611.5 59 28219.0 60 23619.5 61 21386.0 62 19265.0 63 14619.5 64 10576.0 65 9057.0 66 7983.5 67 6195.0 68 4801.5 69 3572.0 70 3021.0 71 2617.0 72 1870.5 73 1442.5 74 1357.0 75 1165.5 76 738.0 77 400.5 78 299.0 79 240.5 80 174.0 81 125.0 82 73.0 83 62.0 84 59.5 85 50.5 86 31.0 87 18.0 88 22.0 89 19.0 90 16.0 91 20.0 92 18.0 93 12.5 94 9.5 95 9.0 96 8.0 97 10.0 98 10.0 99 20.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003588845025227469 2 0.009288775359412273 3 0.01234984905740041 4 0.02174417868226055 5 0.016360911144419347 6 0.01583313981718001 7 0.02068863602778188 8 0.021849732947708415 9 0.021321961620469083 10-11 0.024013595389389686 12-13 0.022483058540395617 14-15 0.009183221093964408 16-17 0.007177690050454938 18-19 0.012877620384639743 20-21 0.010238763748443075 22-23 0.015410922755388544 24-25 0.02195528721315628 26-27 0.014249825835462011 28-29 0.008655449766725073 30-31 0.00860267263400114 32-33 0.010502649412062741 34-35 0.012772066119191877 36-37 0.010660980810234541 38-39 0.018577550718824547 40-41 0.012930397517363676 42-43 0.016624796808039015 44-45 0.019105322046063883 46-47 0.017574785197069814 48-49 0.014830374295425277 50-51 0.028130211741856488 52-53 0.031719056767083954 54-55 0.02580801790200342 56-57 0.016308134011695414 58-59 0.017258122400726213 60-61 0.02116363022229728 62-63 0.02675800629103422 64-65 0.02148029301864088 66-67 0.021058075956849415 68-69 0.030821845510777088 70-71 0.054518778103823176 72-73 0.06502142751588592 74-75 0.07673795098059913 76-77 0.07235744896451266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 947380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.822208071682965 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2268353350823 39.736475486439495 2 11.565139017628615 12.217923591342355 3 4.9369528600661985 7.823422536435211 4 2.651619977178049 5.602576886461205 5 1.6292756084525992 4.303096759790052 6 1.0330743833173142 3.274156201746764 7 0.6590951143580168 2.437040148875419 8 0.44551289659823323 1.8826379938184041 9 0.3228285707397403 1.5347266141588725 >10 1.4333485840976439 12.58446564728871 >50 0.06488234885963459 2.343354891791409 >100 0.03043417935170324 2.7397954967110136 >500 6.00674561926998E-4 0.17271128760148172 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 4.0044970795133204E-4 3.3476164575395586 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18240 1.9253098017690893 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13412 1.415693808186789 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.166627963436002E-4 0.0 2 0.0 0.0 0.0 0.003377736494331736 0.0 3 0.0 0.0 0.0 0.006016593130528405 0.0 4 0.0 0.0 0.0 0.010872089341130275 0.0 5 0.0 0.0 0.0 0.016255356878971478 0.0 6 0.0 0.0 0.0 0.02786632607823682 0.0 7 0.0 0.0 0.0 0.05108826447676751 0.0 8 0.0 0.0 0.0 0.07399354007895459 0.0 9 0.0 0.0 0.0 0.10312651734256581 0.0 10 0.0 0.0 0.0 0.14049272731111065 0.0 11 0.0 0.0 0.0 0.18429774747197533 0.0 12 0.0 0.0 0.0 0.23591378327598217 0.0 13 0.0 0.0 0.0 0.2976630285629842 0.0 14 0.0 0.0 0.0 0.36310667314066164 0.0 15 0.0 0.0 0.0 0.43340581392894084 0.0 16 0.0 0.0 0.0 0.5034938461863244 0.0 17 0.0 0.0 0.0 0.5650319829424307 0.0 18 0.0 0.0 0.0 0.6266756739639849 0.0 19 0.0 0.0 0.0 0.6976081403449513 0.0 20 0.0 0.0 0.0 0.7743460913255504 0.0 21 0.0 0.0 0.0 0.8605839261964576 0.0 22 0.0 0.0 0.0 0.949777280499905 0.0 23 0.0 0.0 0.0 1.0371762122907386 0.0 24 0.0 0.0 0.0 1.1239418184888852 0.0 25 0.0 0.0 0.0 1.2126074014650932 0.0 26 0.0 0.0 0.0 1.3100339884734742 0.0 27 0.0 0.0 0.0 1.4015495366167747 0.0 28 0.0 0.0 0.0 1.4905317823893263 0.0 29 0.0 0.0 0.0 1.5848972956997192 0.0 30 0.0 0.0 0.0 1.6871793789187022 0.0 31 0.0 0.0 0.0 1.8010724313369504 0.0 32 0.0 0.0 0.0 1.9294264181215564 0.0 33 0.0 0.0 0.0 2.061052587135046 0.0 34 0.0 0.0 0.0 2.1942620701302538 0.0 35 0.0 0.0 0.0 2.323249382507547 0.0 36 0.0 0.0 0.0 2.4725031138508307 0.0 37 0.0 0.0 0.0 2.6153180350017946 0.0 38 0.0 0.0 0.0 2.76151069264709 0.0 39 0.0 0.0 0.0 2.9093922185395513 0.0 40 0.0 0.0 0.0 3.07669572927442 0.0 41 0.0 0.0 0.0 3.24146593763854 0.0 42 0.0 0.0 0.0 3.4265025649686502 0.0 43 0.0 0.0 0.0 3.622516835905339 0.0 44 0.0 0.0 0.0 3.8303531845721888 0.0 45 0.0 0.0 0.0 4.042306149591505 0.0 46 0.0 0.0 0.0 4.26967003736621 0.0 47 0.0 0.0 0.0 4.501678312820621 0.0 48 0.0 0.0 0.0 4.729042200595326 0.0 49 0.0 0.0 0.0 4.969494817285566 0.0 50 0.0 1.0555426544786674E-4 0.0 5.227152779243809 0.0 51 0.0 1.0555426544786674E-4 0.0 5.490932888598028 0.0 52 0.0 1.0555426544786674E-4 0.0 5.762629567860837 0.0 53 0.0 1.0555426544786674E-4 0.0 6.0616648018746435 0.0 54 0.0 1.0555426544786674E-4 0.0 6.383605311490637 0.0 55 0.0 1.0555426544786674E-4 0.0 6.696257045747219 0.0 56 0.0 1.0555426544786674E-4 0.0 7.027380776457177 0.0 57 0.0 1.0555426544786674E-4 0.0 7.36219890645781 0.0 58 0.0 1.0555426544786674E-4 0.0 7.710950199497562 0.0 59 0.0 1.0555426544786674E-4 0.0 8.053790453672233 0.0 60 0.0 1.0555426544786674E-4 0.0 8.41710823534379 0.0 61 0.0 1.0555426544786674E-4 0.0 8.797948025079693 0.0 62 0.0 1.0555426544786674E-4 0.0 9.185648842069709 0.0 63 0.0 1.0555426544786674E-4 0.0 9.587599484895184 0.0 64 0.0 1.0555426544786674E-4 0.0 9.998627794549177 0.0 65 0.0 1.0555426544786674E-4 0.0 10.441005721041186 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACGG 45 0.0040194555 31.555086 24 CGGTTAC 180 9.094947E-12 25.635801 4 ACGGTTA 80 0.002459192 22.182486 1 GCCTAGT 265 7.8216544E-11 18.752459 17 CGATCGT 175 2.4462734E-6 18.253016 1 CCACTCG 100 0.008921838 17.752548 49 TAGTGTA 100 0.008924553 17.751612 54 TCGCGCT 180 3.2011922E-6 17.745987 37 GAGACGT 145 2.1921782E-4 17.134058 37 CGCGGAA 190 5.3343974E-6 16.81998 59 CGCCGTA 130 0.0020754985 16.393894 71 AGGCAAT 230 1.9092167E-6 15.436184 55 TTGCGCG 255 3.3243305E-7 15.31188 5 TAAGCCG 165 5.8636436E-4 15.0572 29 GGCAGCG 190 1.0065084E-4 14.94399 2 CAATTCG 215 1.7255446E-5 14.861813 54 CGCGCTT 240 2.9799303E-6 14.7891035 38 TCGCGGA 225 2.649047E-5 14.202038 58 CCCTACC 175 9.1394526E-4 14.200539 12 AGTCACG 225 2.6556505E-5 14.198289 4 >>END_MODULE