FastQCFastQC Report
Thu 26 May 2016
SRR936080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947380
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80400.8486562942008486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65180.6880027021891955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52200.5509932656378644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47700.5034938461863244No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32810.34632354493445083No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17800.18788659249720283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.18334775908294454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13660.14418712660178598No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.14007051024931919No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT11820.12476514175937849RNA PCR Primer, Index 23 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9700.10238763748443075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA7000.054.430134100-101
CGTACAT6400.046.045017100-101
CCGTACA2600.045.794582100-101
ACGTATC1950.045.794582100-101
TCGGTAT1500.045.641933100-101
ACCGGTA1000.044.6391198-99
TATACGT1000.044.6379396-97
AGTATCA12800.044.417164100-101
CCCCGTA1150.043.9921698-99
AGGTATC9850.042.609882100-101
GAGCGTA1050.042.51343598-99
TTTACGC1500.041.66316698-99
GGTATCA56250.041.366731
CGGTATC7400.040.62722100-101
CCCGTAT1700.040.26272298-99
TCCGTAC1650.039.67920798-99
ACCGTAT1450.038.99508398-99
CCGGTAT3450.038.825844100-101
AACGTAT1000.038.6872398-99
AGCGTAC950.037.59082898-99
Produced by FastQC (version 0.11.5)