FastQCFastQC Report
Thu 26 May 2016
SRR936079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937174
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115700.2938656000471404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40150.10197669699129376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG1407.321025E-620.28105711
TTGCGCG3800.017.7447995
CTCGCGC1653.0151545E-517.20838736
CGATTCG1300.002085481816.38397815
CGGTATA1754.993232E-516.22484610
TCGCGCT1806.3452106E-515.77656237
CGGTTAC3703.45608E-1115.3464634
GCGTTCG1655.869632E-415.05714910
CGAACTC9350.014.8057825
TCGAACT10050.014.12933924
CGCGGAA1800.001132152913.80571859
CTCGAAC10750.013.54038223
ATTTGCG5250.013.5195033
TAAGGCG8750.013.3883917
CCTCGCG3002.0869538E-613.01400935
ATTCGCG2203.4898403E-412.908751556
GCGGTTA5002.3646862E-1112.775933
CGCGCTT2254.2227076E-412.62044738
CGATCGT2254.226781E-412.6190041
TTTGCGC5407.2759576E-1212.4867634
Produced by FastQC (version 0.11.5)