##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936079_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3937174 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.19317612074041 39.0 36.0 41.0 29.0 41.0 2 36.04595301096675 39.0 36.0 41.0 28.0 41.0 3 35.94773738727321 39.0 35.0 41.0 27.0 41.0 4 35.82262150466299 39.0 35.0 41.0 27.0 41.0 5 35.74828163550811 39.0 35.0 40.0 27.0 41.0 6 35.64041060923393 39.0 35.0 40.0 27.0 41.0 7 35.46259804621284 38.0 35.0 40.0 27.0 41.0 8 35.265472392126945 38.0 34.0 40.0 26.0 41.0 9 35.16626646422028 38.0 34.0 40.0 26.0 41.0 10-11 35.366830498220295 38.0 35.0 40.0 26.5 41.0 12-13 35.53073448112784 39.0 35.0 41.0 27.0 41.0 14-15 35.462989062713504 39.0 35.0 41.0 26.5 41.0 16-17 35.284034309888256 39.0 35.0 41.0 25.5 41.0 18-19 35.11527951266568 39.0 35.0 41.0 25.0 41.0 20-21 34.87702816791943 39.0 34.0 41.0 24.0 41.0 22-23 34.57082999125769 39.0 34.0 41.0 23.0 41.0 24-25 34.31450502314604 38.0 33.5 40.0 21.5 41.0 26-27 33.385812641249785 37.5 32.0 39.5 19.0 40.5 28-29 33.42095802725508 38.0 33.0 39.5 19.0 40.5 30-31 33.79782110214077 38.0 33.0 40.0 18.5 41.0 32-33 33.64441856011443 38.0 33.0 40.0 17.5 41.0 34-35 33.372095442060726 38.0 33.0 40.0 14.0 41.0 36-37 33.08210076567609 37.5 32.5 40.0 12.5 41.0 38-39 32.804168167320015 37.0 32.0 40.0 8.5 41.0 40-41 32.429622744638664 36.5 32.0 40.0 7.5 41.0 42-43 32.06800563043442 36.0 31.0 39.0 6.5 41.0 44-45 31.693965265441662 35.5 31.0 39.0 2.0 40.5 46-47 31.30012643586491 35.0 31.0 38.5 2.0 40.0 48-49 30.828768299292843 35.0 30.0 37.5 2.0 40.0 50-51 30.386707191503348 35.0 30.0 37.0 2.0 39.0 52-53 29.927190162283914 35.0 29.5 36.5 2.0 39.0 54-55 29.460723224322827 34.0 29.0 36.0 2.0 38.5 56-57 28.999126657851544 34.0 29.0 35.5 2.0 37.0 58-59 28.561555191617135 34.0 28.5 35.0 2.0 37.0 60-61 28.193983298680728 34.0 28.0 35.0 2.0 36.5 62-63 27.843925363725354 34.0 27.0 35.0 2.0 36.0 64-65 27.45491880724601 34.0 26.0 35.0 2.0 36.0 66-67 27.056322631410247 33.0 26.0 35.0 2.0 35.0 68-69 26.679899465962134 33.0 25.0 35.0 2.0 35.0 70-71 26.245825686139348 33.0 24.5 35.0 2.0 35.0 72-73 25.751031069492992 33.0 23.5 35.0 2.0 35.0 74-75 25.150983167114283 32.5 19.0 35.0 2.0 35.0 76-77 23.476199299294365 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2913925560635917 1101 2 1.1834501888650593 1101 3 1.228935742345307 1101 4 1.2303577399002918 1101 5 1.3157665930873677 1101 6 1.2607821031706266 1101 7 1.287426989489326 1101 8 1.3335048628545891 1101 9 1.3282505882221116 1101 10-11 1.335383569250368 1101 12-13 1.3720997717951988 1101 14-15 1.4516392885987344 1101 16-17 1.4997050726105243 1101 18-19 1.4542102172473221 1101 20-21 1.4225015191241255 1101 22-23 1.4720943043844414 1101 24-25 1.4716414607077226 1101 26-27 1.4427619382321168 1101 28-29 1.515651384605306 1101 30-31 1.5610129070109835 1101 32-33 1.6672684771147814 1101 34-35 1.607021277558701 1101 36-37 1.5734549494521417 1101 38-39 1.6831647283152975 1101 40-41 1.656943669327255 1101 42-43 1.6031793745574063 1101 44-45 1.6021251104039784 1101 46-47 1.6736930920535187 1101 48-49 1.7062343473358368 1101 50-51 1.6430512995109758 1101 52-53 1.7925138018127704 1101 54-55 1.6856337474773575 1101 56-57 1.7063288450448724 1101 58-59 1.74520839191635 1101 60-61 1.555094302736176 1101 62-63 1.5128579865124365 1101 64-65 1.5607726313823598 1101 66-67 1.5298698343594879 1101 68-69 1.533467750408274 1101 70-71 1.5223671548783706 1101 72-73 1.6177076947976659 1101 74-75 1.581130273276571 1101 76-77 1.6178004723618358 1102 1 1.3217378666471689 1102 2 1.4226105475777757 1102 3 1.277706719467325 1102 4 1.4505863745034375 1102 5 1.4300406522164195 1102 6 1.4054403306053231 1102 7 1.301466874143884 1102 8 1.405244080063511 1102 9 1.3382932682134978 1102 10-11 1.2886980573444902 1102 12-13 1.5495582257840326 1102 14-15 1.3616520617518972 1102 16-17 1.3691537102618128 1102 18-19 1.487293182868676 1102 20-21 1.4433969945733054 1102 22-23 1.4439881511356276 1102 24-25 1.3444129627869827 1102 26-27 1.3483926338367453 1102 28-29 1.3987441478157194 1102 30-31 1.4801135488135557 1102 32-33 1.368434601890101 1102 34-35 1.45937607063604 1102 36-37 1.3712335426876976 1102 38-39 1.5684676189669489 1102 40-41 1.608298488410533 1102 42-43 1.6279080811305064 1102 44-45 1.5809022170670666 1102 46-47 1.3820198483015957 1102 48-49 1.4805517970838764 1102 50-51 1.5990527915265424 1102 52-53 1.5830285713758947 1102 54-55 1.6075674498532102 1102 56-57 1.6677916613799937 1102 58-59 1.5348887032377654 1102 60-61 1.4412500221090347 1102 62-63 1.5539076923428468 1102 64-65 1.5369886059279807 1102 66-67 1.6195891394806026 1102 68-69 1.5928252644333405 1102 70-71 1.6703688851809844 1102 72-73 1.7233250245554856 1102 74-75 1.669136890277926 1102 76-77 1.7982324492733923 1103 1 0.9840079661419381 1103 2 0.7934627774608458 1103 3 0.8537006900097595 1103 4 0.97241948113831 1103 5 1.0929461989325375 1103 6 0.999319577292944 1103 7 0.9686213634349343 1103 8 1.091143585896937 1103 9 0.9025073371081831 1103 10-11 0.9648893667957097 1103 12-13 0.9699673338308017 1103 14-15 1.0745441689171287 1103 16-17 1.0547122664150876 1103 18-19 1.1387774038738172 1103 20-21 1.0984163511345102 1103 22-23 1.1681279522302788 1103 24-25 1.0358913103776715 1103 26-27 0.7637074906438954 1103 28-29 0.7746535348759878 1103 30-31 1.0562997169600692 1103 32-33 1.112358767345107 1103 34-35 1.229317079426167 1103 36-37 1.139038655068795 1103 38-39 1.1833333858150894 1103 40-41 1.2169290703340678 1103 42-43 1.156588932085583 1103 44-45 1.0852867953778578 1103 46-47 0.8638924272281372 1103 48-49 1.0132984880078837 1103 50-51 1.0991079252545894 1103 52-53 0.9608009297374629 1103 54-55 1.0414560967099185 1103 56-57 1.064281563376504 1103 58-59 1.0278729645315003 1103 60-61 1.1183383915156853 1103 62-63 1.0909103162122094 1103 64-65 1.0481342254161135 1103 66-67 1.0245853451376021 1103 68-69 0.9446248263099974 1103 70-71 1.0275845184153418 1103 72-73 1.0170758363404389 1103 74-75 0.9519891132457872 1103 76-77 1.0366400098175177 1104 1 0.6497808414437785 1104 2 0.6756867349419764 1104 3 0.6238712641868247 1104 4 0.6444004890695041 1104 5 0.7043898766999845 1104 6 0.47603030386831335 1104 7 0.7564137578867758 1104 8 0.8028888498890865 1104 9 0.6283399029515806 1104 10-11 0.6232344766838622 1104 12-13 0.76637390676688 1104 14-15 0.6824824832712508 1104 16-17 0.7275462339160796 1104 18-19 0.6913466217110411 1104 20-21 0.6696210893668635 1104 22-23 0.7478723370639955 1104 24-25 0.7713946559243254 1104 26-27 0.6392958934385788 1104 28-29 0.7002904850121254 1104 30-31 0.8470978119000208 1104 32-33 0.8262008271535919 1104 34-35 0.9564220452966978 1104 36-37 0.7790408662675432 1104 38-39 0.8379604743891207 1104 40-41 0.9410888628479555 1104 42-43 0.9234186155368107 1104 44-45 0.8241673553195987 1104 46-47 0.6797184171201849 1104 48-49 0.8361019480609357 1104 50-51 0.737223855330452 1104 52-53 0.6638516080182413 1104 54-55 0.6327889514428797 1104 56-57 0.6834075309052849 1104 58-59 0.6426587893877063 1104 60-61 0.5316261440021357 1104 62-63 0.6337889112023376 1104 64-65 0.6734674387298369 1104 66-67 0.8027245489671131 1104 68-69 0.7511315964063705 1104 70-71 0.5475918762678518 1104 72-73 0.41539823709922175 1104 74-75 0.42338036224938236 1104 76-77 0.459459787807841 1105 1 0.24661377574022936 1105 2 0.4716968410332498 1105 3 0.35554241553569454 1105 4 0.41909638458213294 1105 5 0.3626098426563331 1105 6 0.17590852831798287 1105 7 0.30043948660644304 1105 8 0.4831380361620674 1105 9 0.24503492963577855 1105 10-11 0.31954215941843245 1105 12-13 0.4739690897122628 1105 14-15 0.12557473028603283 1105 16-17 0.31015966283992213 1105 18-19 0.4871076084070296 1105 20-21 0.4308749326532464 1105 22-23 0.3042556090346267 1105 24-25 0.2525410354860398 1105 26-27 0.1361680529540834 1105 28-29 0.2534006029087763 1105 30-31 0.24328386447053418 1105 32-33 0.34911326913719876 1105 34-35 0.439306081708402 1105 36-37 0.5052054394686607 1105 38-39 0.300257958566732 1105 40-41 0.41799904959953693 1105 42-43 0.1994521375427709 1105 44-45 0.26551627541936895 1105 46-47 0.21432929364245012 1105 48-49 0.134340680478644 1105 50-51 0.3030355004227623 1105 52-53 0.16664724084693106 1105 54-55 0.1390176632564497 1105 56-57 0.20343674482491636 1105 58-59 0.20931287529471732 1105 60-61 -0.14220540098299495 1105 62-63 -0.008774333284463864 1105 64-65 0.16602111679924292 1105 66-67 0.26854968590645 1105 68-69 -0.004182417610032019 1105 70-71 -0.26225871315721605 1105 72-73 -0.26565362684015525 1105 74-75 -0.24275486909217747 1105 76-77 0.04895049675206309 1106 1 0.0985394670125288 1106 2 0.1517793946867485 1106 3 -0.13479113973477297 1106 4 -0.06510567409743118 1106 5 -0.05396045148383166 1106 6 0.057954935604321633 1106 7 0.12016032139937494 1106 8 0.1589226844535716 1106 9 0.10688312463732075 1106 10-11 -0.053146929858300496 1106 12-13 0.07469684244799879 1106 14-15 0.02576681803394365 1106 16-17 0.147871324746923 1106 18-19 0.1512470819752494 1106 20-21 -0.004613133779422185 1106 22-23 0.10541115139252355 1106 24-25 0.23402866577743708 1106 26-27 0.19876780866857757 1106 28-29 0.1666135662798638 1106 30-31 0.17349241771108836 1106 32-33 0.11866176433384368 1106 34-35 0.1711232828414424 1106 36-37 0.15901178694797835 1106 38-39 0.1790868090793154 1106 40-41 0.14973838907751968 1106 42-43 0.15910515584910456 1106 44-45 0.051046709031940196 1106 46-47 0.288464898776855 1106 48-49 0.10591305672936357 1106 50-51 0.014724086281116655 1106 52-53 -0.06653797651613402 1106 54-55 0.0771910143292871 1106 56-57 0.02295835817640679 1106 58-59 0.01763357483746475 1106 60-61 0.13873119498084208 1106 62-63 0.006412604284694368 1106 64-65 -0.11034556548808538 1106 66-67 -0.24522066251937957 1106 68-69 -0.3005053175782493 1106 70-71 -0.4892047035574656 1106 72-73 -0.3969881147105525 1106 74-75 -0.5118417041217427 1106 76-77 -0.3998127643782361 1107 1 0.007246364671111394 1107 2 -0.05322869419831733 1107 3 0.014282992747652656 1107 4 0.02623313407318051 1107 5 -0.018863747718448565 1107 6 -0.15672890287029873 1107 7 -0.23039103743840883 1107 8 -0.09033597397497317 1107 9 -0.2587958867434992 1107 10-11 -0.13614592446961638 1107 12-13 -0.0897277497722655 1107 14-15 -0.09641630729018402 1107 16-17 0.004807125013435609 1107 18-19 -0.04379221666427213 1107 20-21 -0.19225855507412604 1107 22-23 -0.39139725591214614 1107 24-25 -0.46847701151455823 1107 26-27 -0.4509161651790379 1107 28-29 -0.3550049071370651 1107 30-31 -0.1481468640696093 1107 32-33 -0.04894315112921532 1107 34-35 -0.03351483305682024 1107 36-37 0.08285711814288277 1107 38-39 0.06271080124833617 1107 40-41 0.059636883059972945 1107 42-43 0.0055978719448646075 1107 44-45 0.0907271143358841 1107 46-47 -0.04296265266906829 1107 48-49 0.09583471205353788 1107 50-51 -0.01159626092129784 1107 52-53 -0.05903618456850879 1107 54-55 -0.09068178613874167 1107 56-57 -0.0065163192590631525 1107 58-59 0.04214589015102632 1107 60-61 -0.0066784813688549605 1107 62-63 0.009911368064130954 1107 64-65 0.01089082115280604 1107 66-67 0.013616611889204933 1107 68-69 -0.03701902765228482 1107 70-71 -0.046916860945177774 1107 72-73 -0.019309264660840597 1107 74-75 0.054914520384173215 1107 76-77 0.17797960359960285 1108 1 -0.21204325636703203 1108 2 -0.1540847747617704 1108 3 -0.2782870528179515 1108 4 -0.24353053702080274 1108 5 -0.2609580170317827 1108 6 -0.24266174971278787 1108 7 -0.37546045277634477 1108 8 -0.24511743518299056 1108 9 -0.3428058835453882 1108 10-11 -0.5070770886025429 1108 12-13 -0.3067290318714697 1108 14-15 -0.12994983072200483 1108 16-17 -0.24349298645238804 1108 18-19 -0.29535284762093994 1108 20-21 -0.25464936900031176 1108 22-23 -0.29493706734643155 1108 24-25 -0.28432899866873385 1108 26-27 -0.4487464690486931 1108 28-29 -0.3598462242063576 1108 30-31 -0.4335300235760613 1108 32-33 -0.2676782647597449 1108 34-35 -0.2801712403626908 1108 36-37 -0.2611197874734117 1108 38-39 -0.31530546974644835 1108 40-41 -0.23315385008021394 1108 42-43 -0.15654269661244768 1108 44-45 -0.3469638004997613 1108 46-47 -0.3178516665375284 1108 48-49 -0.30984747513294053 1108 50-51 -0.23211058513166094 1108 52-53 -0.27954015160028334 1108 54-55 -0.2145143379145118 1108 56-57 -0.3427533584285207 1108 58-59 -0.4775848670244578 1108 60-61 -0.4335368496737111 1108 62-63 -0.387404675331652 1108 64-65 -0.38823125525966873 1108 66-67 -0.43356426700636064 1108 68-69 -0.2869588929439004 1108 70-71 -0.3559686632369967 1108 72-73 -0.2552601336580018 1108 74-75 -0.22705488650203876 1108 76-77 -0.14815287918806064 1109 1 0.168157570291541 1109 2 0.1871611511592164 1109 3 0.05687529318436191 1109 4 0.11474622346504759 1109 5 -0.01948077349443622 1109 6 0.03312169109505447 1109 7 -0.15601496120139302 1109 8 -0.01516584041248592 1109 9 -0.012757278156428242 1109 10-11 -0.11155538964904821 1109 12-13 0.05464382850729521 1109 14-15 0.018376702749662854 1109 16-17 0.19479104649386159 1109 18-19 0.04029528539170002 1109 20-21 0.059108991827152124 1109 22-23 0.18661280071512465 1109 24-25 0.0390838535702116 1109 26-27 -0.032306705370295674 1109 28-29 0.20350264372509486 1109 30-31 0.23378967445002985 1109 32-33 0.18516053014686662 1109 34-35 0.28617601628510414 1109 36-37 0.16507508110522195 1109 38-39 0.24920679168850057 1109 40-41 0.30136688477188756 1109 42-43 0.36664821214485954 1109 44-45 0.3346955547866166 1109 46-47 0.08850613184184652 1109 48-49 0.22881192352132018 1109 50-51 0.2638288799755415 1109 52-53 0.29417287310940665 1109 54-55 0.42762473287855585 1109 56-57 0.3913456404405764 1109 58-59 0.33838425504279 1109 60-61 0.21216819534548748 1109 62-63 0.23763080293269923 1109 64-65 0.3691306764125599 1109 66-67 0.45316886372111753 1109 68-69 0.4476757993609688 1109 70-71 0.36268416351498445 1109 72-73 0.31996105922566187 1109 74-75 0.21401009526677228 1109 76-77 0.4385428217203291 1110 1 -0.24719794759587188 1110 2 -0.31462644299344333 1110 3 -0.4204044437617185 1110 4 -0.3292044284345721 1110 5 -0.2699259514764094 1110 6 -0.49802623850233374 1110 7 -0.4600926793615443 1110 8 -0.35775291967277667 1110 9 -0.31493282586681914 1110 10-11 -0.2819664751288258 1110 12-13 -0.25119835188345974 1110 14-15 -0.37883701581832696 1110 16-17 -0.5890695629653706 1110 18-19 -0.3707966942675043 1110 20-21 -0.5293455663722497 1110 22-23 -0.5791473027731442 1110 24-25 -0.5607001847663255 1110 26-27 -0.35735577308374644 1110 28-29 -0.2214321663127521 1110 30-31 -0.40381320843744817 1110 32-33 -0.10819149564873243 1110 34-35 0.02839929349856618 1110 36-37 -0.04324826875394905 1110 38-39 -0.09142611809063084 1110 40-41 -0.10343869237912884 1110 42-43 -0.14496987536135464 1110 44-45 -0.14440599626292183 1110 46-47 -0.050717260075366255 1110 48-49 -0.04588302823938406 1110 50-51 -0.1635649871975211 1110 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-0.8652836666495816 2314 24-25 -0.8965246068606447 2314 26-27 -0.7461926041862341 2314 28-29 -0.8325207301023667 2314 30-31 -1.0650592484247312 2314 32-33 -1.0855037786337007 2314 34-35 -1.026743335622406 2314 36-37 -1.1611617606321545 2314 38-39 -1.0241842306368625 2314 40-41 -0.8227046626258172 2314 42-43 -0.8442179615092584 2314 44-45 -0.8709363913081916 2314 46-47 -0.8291746588421738 2314 48-49 -0.8465846067514242 2314 50-51 -0.8680253691122921 2314 52-53 -0.7071114292405589 2314 54-55 -0.7177913687808193 2314 56-57 -0.5286668013694964 2314 58-59 -0.6156042537573434 2314 60-61 -0.5785251909237878 2314 62-63 -0.5857385510717457 2314 64-65 -0.5551212073334 2314 66-67 -0.6166231203980992 2314 68-69 -0.6275494812787343 2314 70-71 -0.4087719476504894 2314 72-73 -0.4382545894959087 2314 74-75 -0.4763068214010602 2314 76-77 -0.4741893438535989 2315 1 -1.5809668169389397 2315 2 -1.4895000307102109 2315 3 -1.4502684179926675 2315 4 -1.3185411818083281 2315 5 -1.2563489732473343 2315 6 -1.2919751848479422 2315 7 -1.4447832731174444 2315 8 -1.4333142943259602 2315 9 -1.3860635860528276 2315 10-11 -1.4043832676018724 2315 12-13 -1.4907186520634284 2315 14-15 -1.77212398901802 2315 16-17 -1.7040532744635684 2315 18-19 -1.6352186439056524 2315 20-21 -1.6567614682695293 2315 22-23 -1.5993650399448711 2315 24-25 -1.7394196508166786 2315 26-27 -1.5990099057210259 2315 28-29 -1.6055998802305638 2315 30-31 -1.7163796520586274 2315 32-33 -1.8157331881471137 2315 34-35 -1.8053504843952766 2315 36-37 -2.009168956312859 2315 38-39 -1.8620235868763828 2315 40-41 -1.8223427549965017 2315 42-43 -2.011452131176874 2315 44-45 -1.8959015289713612 2315 46-47 -1.7321893366528336 2315 48-49 -1.5557187277651394 2315 50-51 -1.517440271740785 2315 52-53 -1.6112054355613203 2315 54-55 -1.571734741491877 2315 56-57 -1.7362470649688717 2315 58-59 -1.5113540688866784 2315 60-61 -1.465426673617202 2315 62-63 -1.5550974946355822 2315 64-65 -1.6216496830788856 2315 66-67 -1.6276635918548692 2315 68-69 -1.538530751650491 2315 70-71 -1.5370125404824648 2315 72-73 -3.1271184855171903 2315 74-75 -3.8079278221424318 2315 76-77 -3.409218925519717 2316 1 -3.7236730648267624 2316 2 -3.607861086876717 2316 3 -3.073599711263249 2316 4 -2.9617882010145777 2316 5 -3.7145990798456197 2316 6 -3.2325107586469812 2316 7 -3.5589325915162817 2316 8 -3.460908590812771 2316 9 -3.0974575094320045 2316 10-11 -2.9924982823727646 2316 12-13 -3.015679957295937 2316 14-15 -3.2202303066792695 2316 16-17 -4.503155938028463 2316 18-19 -4.314200355953258 2316 20-21 -3.64861561233581 2316 22-23 -3.826724683718247 2316 24-25 -3.837710311169161 2316 26-27 -3.5062793081100274 2316 28-29 -3.5361557800429146 2316 30-31 -3.787099846987438 2316 32-33 -3.9679990499990154 2316 34-35 -3.8822495307705793 2316 36-37 -3.6649729530523985 2316 38-39 -3.826092977035927 2316 40-41 -3.7593109305634655 2316 42-43 -4.081960576326942 2316 44-45 -3.939470963626473 2316 46-47 -3.672838824571606 2316 48-49 -3.825303002648404 2316 50-51 -4.188957101166604 2316 52-53 -5.187269930662165 2316 54-55 -4.2969883252961445 2316 56-57 -4.102507766426527 2316 58-59 -4.14220994527744 2316 60-61 -3.979290619811959 2316 62-63 -3.9515632906449554 2316 64-65 -3.890408551482828 2316 66-67 -3.9944756835124693 2316 68-69 -4.007622190320582 2316 70-71 -3.8873407430852644 2316 72-73 -3.8915102092319103 2316 74-75 -3.844143258229046 2316 76-77 -3.804881835813916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 121199.0 3 10077.0 4 13051.0 5 15291.0 6 17391.0 7 18603.0 8 21501.0 9 23190.0 10 28965.0 11 32395.0 12 24970.0 13 20482.0 14 20090.0 15 20616.0 16 21096.0 17 22132.0 18 23321.0 19 24471.0 20 26167.0 21 28101.0 22 31313.0 23 35195.0 24 40799.0 25 47094.0 26 55622.0 27 65217.0 28 76199.0 29 89565.0 30 105809.0 31 126772.0 32 153388.0 33 192106.0 34 252583.0 35 333029.0 36 466648.0 37 623835.0 38 558175.0 39 149549.0 40 1167.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.189378346461993 26.67898121497955 29.32389116357495 21.80774927498351 2 21.890184052819926 26.936827227705667 29.33086527382353 21.84212344565087 3 22.171042136398412 27.030460463277855 28.839183832726555 21.95931356759717 4 21.78180572991631 27.04014782291936 29.137362628988146 22.04068381817618 5 22.1097028835783 27.153742240340023 28.99589148897581 21.74066338710587 6 22.179508705369788 27.02366857742906 29.05413832422743 21.742684392973725 7 22.113823327385717 26.888359271166536 29.02292134922796 21.97489605221979 8 21.8667638084772 27.17671705898088 28.847967731847955 22.108551400693965 9 21.914344693107264 26.888329342500818 29.09304203050307 22.104283933888848 10-11 22.281500186081605 27.070834217168027 28.846675109140353 21.800990487610015 12-13 22.25095692005361 26.86966479601117 28.817176194206763 22.062202089728466 14-15 22.073691837482464 27.06231540685583 28.953146124425068 21.910846631236637 16-17 22.348599487392917 26.93445291875009 28.803191621092918 21.913755972764083 18-19 22.11499623776894 26.97762272722885 28.657035930957854 22.25034510404436 20-21 22.189372149797514 27.157103357816553 28.56471802295265 22.08880646943328 22-23 22.322362513754754 27.25768802269809 28.16023355548829 22.259715908058865 24-25 22.326983391482706 27.47194527811353 27.848036309189023 22.35303502121474 26-27 22.39497062165035 27.491024411162755 27.878628597094885 22.235376370092006 28-29 22.522397234351146 27.505353237470377 27.723610380835538 22.248639147342942 30-31 22.276934480000428 27.38485173078335 27.90643505393513 22.431778735281096 32-33 22.28010072162835 27.659062927140404 27.74842504974229 22.312411301488954 34-35 22.33272555372042 27.43895066316393 27.870996618008736 22.357327165106913 36-37 22.37615530781473 27.423992046282546 28.061163254038746 22.138689391863974 38-39 22.328823025773566 27.40200772255696 28.048306053155102 22.22086319851437 40-41 22.21921353664653 27.370340081242134 28.015364448420087 22.39508193369125 42-43 22.152636132022714 27.59812026107231 27.773309043401678 22.475934563503298 44-45 22.333633417905702 27.348305724536587 27.90886861680995 22.40919224074776 46-47 22.158250809151927 27.37115811616221 27.95918419194049 22.511406882745373 48-49 22.247125957887015 27.37643879939069 27.859942463267235 22.516492779455056 50-51 22.106707140068604 27.482335974547183 27.793686774336855 22.61727011104735 52-53 22.099802115443836 27.39193084305732 27.963542286008742 22.544724755490105 54-55 22.103559237463045 27.383093113936454 27.89974350401562 22.613604144584876 56-57 22.00576541651927 27.545049064469396 27.78524299788957 22.66394252112176 58-59 22.07477738053081 27.593522531727118 27.728742870004048 22.602957217738023 60-61 22.075380363741303 27.337426501624012 27.802661647524584 22.7845314871101 62-63 21.956547558936194 27.370223511979052 27.851697442356315 22.821531486728443 64-65 22.033573418082177 27.282508330730344 27.80931357424122 22.874604676946262 66-67 22.01247659320841 27.35033658397844 27.891203212934258 22.745983609878888 68-69 22.030478341340753 27.16836877298936 28.035243663092718 22.765909222577164 70-71 21.815401892158622 27.111029281991406 28.10662537599782 22.966943449852153 72-73 21.895417027538812 26.93603087328475 28.04216172986791 23.126390369308524 74-75 21.781779282932128 26.867700016930446 27.97164887783693 23.378871822300496 76-77 21.709888508025024 26.79553330453021 28.323229996734543 23.171348190710216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11923.0 1 7274.5 2 2491.0 3 2483.0 4 2610.0 5 2745.0 6 2963.5 7 3263.0 8 3491.0 9 3503.0 10 3595.0 11 3835.5 12 4229.5 13 4475.0 14 4796.0 15 5390.5 16 6038.0 17 6412.0 18 7027.5 19 8415.5 20 10089.0 21 12846.5 22 14703.0 23 17026.0 24 22196.0 25 29440.5 26 33838.0 27 38493.5 28 49731.5 29 61236.5 30 66159.0 31 74316.5 32 90623.5 33 105581.5 34 118070.5 35 123751.0 36 132446.0 37 148115.0 38 156916.5 39 158744.0 40 162722.5 41 169764.5 42 177353.0 43 181878.0 44 181761.0 45 183366.0 46 186053.0 47 187938.0 48 188858.0 49 187129.0 50 182784.0 51 173704.5 52 167241.0 53 162582.5 54 150194.5 55 132387.5 56 113996.5 57 105683.0 58 99923.0 59 85259.5 60 69735.5 61 63115.0 62 54780.5 63 39363.5 64 27535.5 65 22790.0 66 19412.0 67 13868.0 68 10117.0 69 7221.0 70 5910.0 71 4833.5 72 3206.5 73 2414.0 74 2172.0 75 1968.5 76 1398.0 77 843.5 78 656.0 79 535.0 80 357.0 81 258.5 82 183.5 83 150.0 84 133.5 85 110.5 86 86.0 87 68.0 88 64.5 89 66.5 90 71.0 91 70.0 92 67.0 93 55.5 94 55.0 95 57.0 96 51.0 97 60.5 98 72.0 99 116.5 100 159.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009321406673923987 2 0.012140687711541325 3 0.013461431981416113 4 0.014528186968622672 5 0.011886698428873094 6 0.008915023821654822 7 0.0147821762512909 8 0.016788691584369908 9 0.0165601012299685 10-11 0.01851581870651386 12-13 0.014883771964358193 14-15 0.0077847715137812044 16-17 0.006108442248170896 18-19 0.017271271221439538 20-21 0.02388769203494689 22-23 0.020509634575459453 24-25 0.019303185482785368 26-27 0.02392579042734713 28-29 0.007721274193114147 30-31 0.008000662404049199 32-33 0.009727789526193154 34-35 0.011175528437402054 36-37 0.010121472914328907 38-39 0.013321737875948586 40-41 0.010756446120999479 42-43 0.014312296078354677 44-45 0.012851857703012363 46-47 0.024230577566549002 48-49 0.023011429009741503 50-51 0.03607917760302186 52-53 0.03926674310050813 54-55 0.026668874680163997 56-57 0.01959527315785383 58-59 0.016153718377699335 60-61 0.019747666727454767 62-63 0.026198994507227773 64-65 0.025259234161355323 66-67 0.02751973877710256 68-69 0.04211142306639229 70-71 0.05446800166820161 72-73 0.0867881378877337 74-75 0.08716912181173603 76-77 0.09073767123322464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3937174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.94944117243474 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37416277989473 38.851209156906855 2 13.431204740768068 14.223495705924508 3 5.361514124203256 8.516675301440348 4 2.6589905100832723 5.631682463668658 5 1.4522926462577948 3.8449042019093342 6 0.8982379409126211 2.8536718206721043 7 0.6014280307862122 2.229169469489747 8 0.41300157572307344 1.7494562110297385 9 0.3091476557754655 1.4732276051771205 >10 1.3764000216157417 12.69667702658073 >50 0.08141161263187975 2.9737923836155944 >100 0.04047339796462113 3.8569974625977173 >500 0.0012530291344795973 0.44409237472571056 >1k 4.3374082393891254E-4 0.3597038305160811 >5k 0.0 0.0 >10k+ 4.819342488210082E-5 0.2952449857456743 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11570 0.2938656000471404 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4015 0.10197669699129376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.5398928266822854E-5 0.0 2 0.0 0.0 0.0 1.0159571306729142E-4 0.0 3 0.0 0.0 0.0 1.7779249786775998E-4 0.0 4 5.079785653364571E-5 0.0 0.0 7.619678480046856E-4 0.0 5 5.079785653364571E-5 0.0 0.0 0.0018033239069444227 0.0 6 5.079785653364571E-5 0.0 0.0 0.003809839240023428 0.0 7 5.079785653364571E-5 0.0 0.0 0.007949864547515553 0.0 8 5.079785653364571E-5 0.0 0.0 0.012216884496341793 0.0 9 7.619678480046856E-5 2.5398928266822854E-5 0.0 0.016585500158235324 0.0 10 7.619678480046856E-5 2.5398928266822854E-5 0.0 0.02199547187906859 0.0 11 1.2699464133411427E-4 2.5398928266822854E-5 0.0 0.02857379430017571 0.0 12 1.2699464133411427E-4 2.5398928266822854E-5 0.0 0.037006238484760895 0.0 13 1.2699464133411427E-4 2.5398928266822854E-5 0.0 0.04988349511604008 0.0 14 1.7779249786775998E-4 2.5398928266822854E-5 0.0 0.06611341027853988 0.0 15 1.7779249786775998E-4 2.5398928266822854E-5 0.0 0.08328308578691214 0.0 16 1.7779249786775998E-4 2.5398928266822854E-5 0.0 0.10131632485635636 0.0 17 1.7779249786775998E-4 5.079785653364571E-5 0.0 0.11840980357992814 0.0 18 1.7779249786775998E-4 5.079785653364571E-5 0.0 0.13573187265790132 0.0 19 2.0319142613458283E-4 5.079785653364571E-5 0.0 0.1534857235164105 0.0 20 2.285903544014057E-4 5.079785653364571E-5 0.0 0.17474462647574124 0.0 21 2.285903544014057E-4 5.079785653364571E-5 0.0 0.19597813050680513 0.0 22 3.0478713920187425E-4 5.079785653364571E-5 0.0 0.22109767056269294 0.0 23 3.0478713920187425E-4 5.079785653364571E-5 0.0 0.24990005521727005 0.0 24 3.5558499573551996E-4 5.079785653364571E-5 0.0 0.277153105247571 0.0 25 3.5558499573551996E-4 5.079785653364571E-5 0.0 0.306539665252285 0.0 26 3.5558499573551996E-4 5.079785653364571E-5 0.0 0.33905029343381826 0.0 27 3.5558499573551996E-4 5.079785653364571E-5 0.0 0.37074815591081317 0.0 28 3.5558499573551996E-4 5.079785653364571E-5 0.0 0.40209043339207257 0.0 29 3.809839240023428E-4 5.079785653364571E-5 0.0 0.43302632802106283 0.0 30 3.809839240023428E-4 7.619678480046856E-5 0.0 0.4668830994007377 0.0 31 3.809839240023428E-4 7.619678480046856E-5 0.0 0.505413273581508 0.0 32 4.571807088028114E-4 7.619678480046856E-5 0.0 0.5480834730697703 0.0 33 4.571807088028114E-4 7.619678480046856E-5 0.0 0.5935475546673833 0.0 34 4.571807088028114E-4 1.2699464133411427E-4 0.0 0.641475332306878 0.0 35 4.571807088028114E-4 1.2699464133411427E-4 0.0 0.6920445984861223 0.0 36 4.571807088028114E-4 1.2699464133411427E-4 0.0 0.7467792889011255 0.0 37 4.825796370696342E-4 1.2699464133411427E-4 0.0 0.8027585268012031 0.0 38 4.825796370696342E-4 1.2699464133411427E-4 0.0 0.8571376322204708 0.0 39 4.825796370696342E-4 1.2699464133411427E-4 0.0 0.9164187307952354 0.0 40 4.825796370696342E-4 1.2699464133411427E-4 0.0 0.9748362658089279 0.0 41 4.825796370696342E-4 1.2699464133411427E-4 0.0 1.040898878230934 0.0 42 5.079785653364571E-4 1.2699464133411427E-4 0.0 1.1146319669895208 0.0 43 5.079785653364571E-4 1.2699464133411427E-4 0.0 1.1942830060342773 0.0 44 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.2806393621414751 0.0 45 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.3702467810668262 0.0 46 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.4694041970205025 0.0 47 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.5646247790928214 0.0 48 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.6620550679243538 0.0 49 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.7671812320207336 0.0 50 5.333774936032799E-4 1.2699464133411427E-4 0.0 1.8677609879573522 0.0 51 6.095742784037485E-4 1.2699464133411427E-4 0.0 1.9778399430657625 0.0 52 6.095742784037485E-4 1.2699464133411427E-4 0.0 2.096783124139294 0.0 53 6.095742784037485E-4 1.2699464133411427E-4 0.0 2.222787207271002 0.0 54 6.095742784037485E-4 1.2699464133411427E-4 0.0 2.36390365272147 0.0 55 6.095742784037485E-4 1.2699464133411427E-4 0.0 2.5093125170490307 0.0 56 7.365689197378627E-4 1.2699464133411427E-4 0.0 2.6644745698310515 0.0 57 7.619678480046856E-4 1.2699464133411427E-4 0.0 2.823852844705365 0.0 58 7.619678480046856E-4 1.2699464133411427E-4 0.0 2.989758644144252 0.0 59 7.619678480046856E-4 1.2699464133411427E-4 0.0 3.158559921405556 0.0 60 7.619678480046856E-4 1.2699464133411427E-4 0.0 3.330205878632745 0.0 61 8.889624893387998E-4 1.2699464133411427E-4 0.0 3.5092429239855796 0.0 62 8.889624893387998E-4 1.2699464133411427E-4 0.0 3.6939693292701823 0.0 63 9.143614176056228E-4 1.2699464133411427E-4 0.0 3.890607831911925 0.0 64 9.143614176056228E-4 1.2699464133411427E-4 0.0 4.101114149387352 0.0 65 9.143614176056228E-4 1.2699464133411427E-4 0.0 4.318478177494822 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCGG 140 7.321025E-6 20.281057 11 TTGCGCG 380 0.0 17.744799 5 CTCGCGC 165 3.0151545E-5 17.208387 36 CGATTCG 130 0.0020854818 16.383978 15 CGGTATA 175 4.993232E-5 16.224846 10 TCGCGCT 180 6.3452106E-5 15.776562 37 CGGTTAC 370 3.45608E-11 15.346463 4 GCGTTCG 165 5.869632E-4 15.057149 10 CGAACTC 935 0.0 14.80578 25 TCGAACT 1005 0.0 14.129339 24 CGCGGAA 180 0.0011321529 13.805718 59 CTCGAAC 1075 0.0 13.540382 23 ATTTGCG 525 0.0 13.519503 3 TAAGGCG 875 0.0 13.38839 17 CCTCGCG 300 2.0869538E-6 13.014009 35 ATTCGCG 220 3.4898403E-4 12.9087515 56 GCGGTTA 500 2.3646862E-11 12.77593 3 CGCGCTT 225 4.2227076E-4 12.620447 38 CGATCGT 225 4.226781E-4 12.619004 1 TTTGCGC 540 7.2759576E-12 12.486763 4 >>END_MODULE