FastQCFastQC Report
Thu 26 May 2016
SRR936079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937174
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138930.3528673104109699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104610.2656981885992339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72870.18508199028033814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA20000.049.10915100-101
GGTATCA140050.038.748481
CGAGTAC16950.037.577038100-101
CGTACAT27700.037.391937100-101
CGGTATC17300.037.160892100-101
TCGAGTA8050.035.86364100-101
TCGGTAT7500.032.54101100-101
TACGCAG17450.031.72459100-101
CCGTACA12050.031.615597100-101
CCGTATC9400.031.346266100-101
GTATCAA269950.030.9916691
TCGTACA15400.030.922722100-101
GCGTATC5900.030.772041100-101
TCGTATC9600.030.383188100-101
ACGTATC7000.030.18831100-101
AGTATCA50250.029.318895100-101
CCTGTCG10800.028.93250796-97
CCGGTAT6850.028.24238100-101
GCGCGTA1204.3473847E-1027.28234398-99
AAACGCA15550.027.179188100-101
Produced by FastQC (version 0.11.5)