FastQCFastQC Report
Thu 26 May 2016
SRR936078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3954457
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115050.290937542120195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42020.10625984806510728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA1754.461981E-922.31662859
TGCGTCG1153.683273E-521.61807367
ATTCGCG1801.4693069E-719.72154856
ACGCTCG2052.7584974E-819.04981645
GCGTTCG950.006650476318.6823910
TAACGCG2103.63234E-818.59789752
CGCACGA2103.645073E-818.592254
TCGAACT12300.018.47044424
CGAACTC12200.017.45665425
CTCGAAC12450.017.3927623
GCCTCGA12600.016.9039721
TTAGTCG3900.016.38301815
GTAGACC21550.016.1416668
GTAACGT2201.2076416E-616.1327512
CGATCGT2652.9491275E-816.0718751
AACGCGA2452.1097912E-715.94085153
ACGCGAA2452.1122287E-715.939239554
CGTTCGG1400.003420262415.2126111
GCGTAAG3051.0075382E-815.13043938
CGCGAAT2757.8186895E-714.20005355
Produced by FastQC (version 0.11.5)