##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936078_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3954457 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.17982797638209 39.0 36.0 41.0 29.0 41.0 2 36.03869279650784 39.0 36.0 41.0 28.0 41.0 3 35.89857545549237 39.0 35.0 41.0 27.0 41.0 4 35.7903704604703 39.0 35.0 41.0 27.0 41.0 5 35.71419792907092 39.0 35.0 40.0 27.0 41.0 6 35.61142300953077 39.0 35.0 40.0 27.0 41.0 7 35.462103899473426 38.0 35.0 40.0 27.0 41.0 8 35.31119392624575 38.0 34.0 40.0 27.0 41.0 9 35.45871506505191 39.0 35.0 40.0 27.0 41.0 10-11 35.668997791605776 39.0 35.0 40.5 27.0 41.0 12-13 35.56792614004907 39.0 35.0 41.0 27.0 41.0 14-15 35.407708062067684 39.0 35.0 41.0 26.0 41.0 16-17 35.24334314420412 39.0 35.0 41.0 25.0 41.0 18-19 35.06163652809981 39.0 35.0 41.0 25.0 41.0 20-21 34.827511716526445 39.0 34.0 41.0 24.0 41.0 22-23 34.515895481984 38.5 34.0 40.5 23.0 41.0 24-25 34.261949870740786 38.0 33.5 40.0 21.5 41.0 26-27 33.31019138657975 37.5 32.0 39.5 18.0 40.5 28-29 33.32492311839527 38.0 33.0 39.5 18.0 40.5 30-31 33.73595135817636 38.0 33.0 40.0 18.0 41.0 32-33 33.61753143351919 38.0 33.0 40.0 17.0 41.0 34-35 33.36374614770119 38.0 33.0 40.0 13.5 41.0 36-37 33.05591387136084 37.5 32.0 40.0 12.0 41.0 38-39 32.751851265546705 37.0 32.0 40.0 8.0 41.0 40-41 32.396256300169654 36.5 32.0 40.0 7.5 41.0 42-43 32.018476999497025 36.0 31.0 39.0 4.5 41.0 44-45 31.65663971058479 35.5 31.0 39.0 2.0 40.5 46-47 31.235843252309987 35.0 31.0 38.5 2.0 40.0 48-49 30.81512278424067 35.0 30.0 37.5 2.0 40.0 50-51 30.356580562135335 35.0 30.0 37.0 2.0 39.0 52-53 29.86279974722193 34.5 29.0 36.5 2.0 39.0 54-55 29.391469296543114 34.0 29.0 36.0 2.0 38.5 56-57 28.94764540365466 34.0 29.0 35.5 2.0 37.0 58-59 28.510734975750147 34.0 28.5 35.0 2.0 37.0 60-61 28.138711585433853 34.0 27.5 35.0 2.0 36.5 62-63 27.759396422821133 34.0 27.0 35.0 2.0 36.0 64-65 27.365100189482398 34.0 26.0 35.0 2.0 36.0 66-67 26.994150145013588 33.0 26.0 35.0 2.0 35.0 68-69 26.61490692653884 33.0 25.0 35.0 2.0 35.0 70-71 26.151184473620525 33.0 24.5 35.0 2.0 35.0 72-73 25.660788699940348 33.0 21.5 35.0 2.0 35.0 74-75 25.078264474743307 32.5 18.0 35.0 2.0 35.0 76-77 23.357473984418085 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.072150614448887 1101 2 0.9732279482505675 1101 3 1.1872986380500095 1101 4 1.0250988368073806 1101 5 1.1372352572618354 1101 6 1.0996242921323756 1101 7 1.2236167020749207 1101 8 1.1969180007965647 1101 9 1.1415154312499993 1101 10-11 1.1118899834113094 1101 12-13 1.3076488294167277 1101 14-15 1.286354324728869 1101 16-17 1.1877749702404188 1101 18-19 1.1697821441483995 1101 20-21 1.2173857461126971 1101 22-23 1.1261220376726016 1101 24-25 1.2811797546229045 1101 26-27 1.1662077895386247 1101 28-29 1.318764612308975 1101 30-31 1.3104275837702133 1101 32-33 1.3647727349397272 1101 34-35 1.3503822369134681 1101 36-37 1.3597321638686068 1101 38-39 1.3517092906594854 1101 40-41 1.3748138387374809 1101 42-43 1.3714179523499297 1101 44-45 1.2436943777868557 1101 46-47 1.2864761121027612 1101 48-49 1.2874215666292343 1101 50-51 1.1844671458801947 1101 52-53 1.2137562069140984 1101 54-55 1.3211926253365043 1101 56-57 1.2358718544887317 1101 58-59 1.333427512830962 1101 60-61 1.0559412075299193 1101 62-63 1.1194761145034349 1101 64-65 1.108008960116134 1101 66-67 1.2488963421423875 1101 68-69 1.248258764834432 1101 70-71 1.2156959201445225 1101 72-73 1.047579994103387 1101 74-75 1.2121179497589942 1101 76-77 1.4128394963756676 1102 1 0.9997120438923659 1102 2 1.0051536077759025 1102 3 1.0929010180042695 1102 4 0.8736223973248514 1102 5 0.9634456434528005 1102 6 0.9864249599316537 1102 7 0.996825668824691 1102 8 1.0255193731682084 1102 9 0.9506274347213761 1102 10-11 1.075401335263571 1102 12-13 1.1995392603237676 1102 14-15 1.2055059328160098 1102 16-17 1.2927384177909644 1102 18-19 1.1968576172341727 1102 20-21 1.2204761564268125 1102 22-23 1.1379546980584223 1102 24-25 1.2415385496423639 1102 26-27 1.076118136750118 1102 28-29 1.1853529522982384 1102 30-31 1.2710930663434468 1102 32-33 1.3327043958330123 1102 34-35 1.3259483586029717 1102 36-37 1.2759535084397982 1102 38-39 1.3644362725450634 1102 40-41 1.3058790080040055 1102 42-43 1.2848936343068473 1102 44-45 1.2023233667475672 1102 46-47 1.173244603450044 1102 48-49 1.2415250755172877 1102 50-51 1.260354296627792 1102 52-53 1.2164321845266421 1102 54-55 1.294689341499744 1102 56-57 1.3229637651448378 1102 58-59 1.372424085579162 1102 60-61 1.2854310177072392 1102 62-63 1.3211259390958006 1102 64-65 1.2562034014961547 1102 66-67 1.3601216949801653 1102 68-69 1.3284226257144844 1102 70-71 1.292807614721962 1102 72-73 1.3926842090347265 1102 74-75 1.3488743059872519 1102 76-77 1.2327102021983194 1103 1 0.6571272348821324 1103 2 0.6851297686732991 1103 3 0.6384023405205639 1103 4 0.786275321139712 1103 5 0.5430341491784318 1103 6 0.5542139011489198 1103 7 0.6037811622128331 1103 8 0.5587995229071225 1103 9 0.6061322604491934 1103 10-11 0.6474541596649104 1103 12-13 0.7183970047590904 1103 14-15 0.7306605021331976 1103 16-17 0.6897525953670964 1103 18-19 0.637917809889224 1103 20-21 0.5935260293544644 1103 22-23 0.6257968996002674 1103 24-25 0.7315130704325199 1103 26-27 0.7195190429813039 1103 28-29 0.6704909910608805 1103 30-31 0.8202110051602602 1103 32-33 0.7363169837999521 1103 34-35 0.8396864867082954 1103 36-37 0.9226348809218194 1103 38-39 0.817704725729719 1103 40-41 0.785162619232409 1103 42-43 0.860813982642604 1103 44-45 0.8016684748040213 1103 46-47 0.7805448430639643 1103 48-49 0.7661279070041829 1103 50-51 0.7233306740481176 1103 52-53 0.8396863515140183 1103 54-55 0.8415551181444734 1103 56-57 0.8279850110077014 1103 58-59 0.7445738417570809 1103 60-61 0.6191984879065657 1103 62-63 0.49892845435851285 1103 64-65 0.4004533149948841 1103 66-67 0.4837152490709755 1103 68-69 0.4260164876850716 1103 70-71 0.31648565591598654 1103 72-73 0.305655874743465 1103 74-75 0.4270399107803904 1103 76-77 0.39176283885054275 1104 1 0.6534266250159604 1104 2 0.6730687183442967 1104 3 0.8336609584640087 1104 4 0.7782859681358616 1104 5 0.6981153643801363 1104 6 0.666022439275288 1104 7 0.7905725157759278 1104 8 0.6127360444400338 1104 9 0.518338432733735 1104 10-11 0.7301013611118847 1104 12-13 0.8099384830916776 1104 14-15 0.9209457552648246 1104 16-17 0.8211428637011338 1104 18-19 0.6592695324160474 1104 20-21 0.5922216307433885 1104 22-23 0.6065083511383804 1104 24-25 0.5968054557773215 1104 26-27 0.505805372139136 1104 28-29 0.573144882276047 1104 30-31 0.7197608085376999 1104 32-33 0.6735245896062452 1104 34-35 0.7725314787619837 1104 36-37 0.7697028510904076 1104 38-39 0.8284572007669695 1104 40-41 0.8620110305009518 1104 42-43 0.8133668902140769 1104 44-45 0.7470912221396198 1104 46-47 0.6425852931543972 1104 48-49 0.6039025857006308 1104 50-51 0.4801968893029134 1104 52-53 0.3800331221965507 1104 54-55 0.5393967653140663 1104 56-57 0.35373021224937773 1104 58-59 0.30024418130705754 1104 60-61 0.3921906194649267 1104 62-63 0.36002925748464776 1104 64-65 0.3640227841670871 1104 66-67 0.34664830537475666 1104 68-69 0.30032622041290935 1104 70-71 0.2295865581498049 1104 72-73 -0.054577028297366326 1104 74-75 0.0347584520569697 1104 76-77 0.15856743036763632 1105 1 0.17851895232519155 1105 2 0.3120571972181736 1105 3 0.21228604261537498 1105 4 0.2373631299343657 1105 5 0.0944271427876302 1105 6 0.12116011699339424 1105 7 0.27632956492032434 1105 8 0.1277886575246754 1105 9 0.20168263833616606 1105 10-11 0.20034363005876799 1105 12-13 0.40961565497963903 1105 14-15 0.2831534695311504 1105 16-17 0.31386119191144957 1105 18-19 0.22366638241526005 1105 20-21 0.1458294671270437 1105 22-23 0.22851893811914437 1105 24-25 0.22731123373601747 1105 26-27 0.18801016393953063 1105 28-29 0.264418433785373 1105 30-31 0.37831794535607344 1105 32-33 0.37181153749067875 1105 34-35 0.3264838530046248 1105 36-37 0.3436758913421585 1105 38-39 0.21035374199374246 1105 40-41 0.3430677454752882 1105 42-43 0.37413609020642724 1105 44-45 0.19172830579694633 1105 46-47 0.1312080984781545 1105 48-49 -0.004339081357436214 1105 50-51 0.05534305608874135 1105 52-53 0.05320919902957044 1105 54-55 -0.042212469678286624 1105 56-57 0.013355092871865537 1105 58-59 0.21833915101257162 1105 60-61 0.14228947883342968 1105 62-63 -0.027437573396767334 1105 64-65 -0.09461670356638407 1105 66-67 0.13518820312323854 1105 68-69 0.0407075326349009 1105 70-71 -0.008202248457752859 1105 72-73 -0.39587996102672207 1105 74-75 -0.6139854964380476 1105 76-77 -0.5011562922277228 1106 1 0.17600070040943194 1106 2 0.22635729915168668 1106 3 0.11137124638968743 1106 4 0.08038386877552739 1106 5 0.0843601316189222 1106 6 0.08833227434664792 1106 7 -0.23381433233367233 1106 8 0.021527952145333984 1106 9 0.04219773857722231 1106 10-11 -0.06682166234235609 1106 12-13 0.23798358084808768 1106 14-15 0.2145472677913034 1106 16-17 0.1014665346362662 1106 18-19 0.1073451448380851 1106 20-21 0.050427000003864464 1106 22-23 0.08218562553229702 1106 24-25 -0.04208461670369701 1106 26-27 -0.21587585386922825 1106 28-29 -0.03499255883638597 1106 30-31 0.26438961917821757 1106 32-33 0.07184214718181892 1106 34-35 -0.011479601050552901 1106 36-37 -0.03164396476679343 1106 38-39 0.10510444532393848 1106 40-41 0.05464243384540168 1106 42-43 0.1697738404678759 1106 44-45 0.04868858494961259 1106 46-47 0.08707765666817124 1106 48-49 -0.05798682122703269 1106 50-51 -0.11740763993538295 1106 52-53 -0.06786336427256145 1106 54-55 -0.05615483592389836 1106 56-57 -0.030347874710468403 1106 58-59 -0.14682719277389467 1106 60-61 -0.36593922773584 1106 62-63 -0.264684598015549 1106 64-65 -0.3078928799700158 1106 66-67 -0.39699698075236967 1106 68-69 -0.3628138707487878 1106 70-71 -0.5124548312753667 1106 72-73 -0.49985262473222747 1106 74-75 -0.5374966616692411 1106 76-77 -0.5843515511098225 1107 1 -0.1109160719491058 1107 2 0.014538491903387296 1107 3 -0.022300103529921955 1107 4 -0.14358480976073906 1107 5 -0.09822165993785603 1107 6 -0.2674024712177854 1107 7 -0.20609702928308593 1107 8 -0.06746449094094231 1107 9 -0.14650244752293418 1107 10-11 -0.1412884042105773 1107 12-13 -0.043509974864974765 1107 14-15 -0.3182407446322131 1107 16-17 -0.26555976072844345 1107 18-19 -0.21460736317138185 1107 20-21 -0.23733648067696578 1107 22-23 -0.175875621973006 1107 24-25 -0.1436976097105287 1107 26-27 -0.3964895922241496 1107 28-29 -0.30682705339852845 1107 30-31 -0.029733336148012768 1107 32-33 -0.05090572119370762 1107 34-35 -0.15490712845038246 1107 36-37 -0.19906225216637807 1107 38-39 -0.12684645855537013 1107 40-41 -0.20038662604484614 1107 42-43 -0.16048280456562125 1107 44-45 -0.2682774667083798 1107 46-47 -0.26299210128056316 1107 48-49 -0.1957086984830454 1107 50-51 -0.12942219975233016 1107 52-53 -0.20476876691449064 1107 54-55 -0.3011028128907469 1107 56-57 -0.36524406156544487 1107 58-59 -0.4614475495145456 1107 60-61 -0.43143718447669954 1107 62-63 -0.37910898505754886 1107 64-65 -0.25588093360875774 1107 66-67 -0.3808003983523207 1107 68-69 -0.5716565822998696 1107 70-71 -0.5346499261374937 1107 72-73 -0.3806096294119712 1107 74-75 -0.5806473680523645 1107 76-77 -0.5509206364819228 1108 1 0.1056245550929944 1108 2 0.3609755050810364 1108 3 0.23946670340353648 1108 4 0.29407444472919764 1108 5 0.21572663616697696 1108 6 0.24686606553033386 1108 7 0.24490299709242436 1108 8 0.31472638002535547 1108 9 0.27855645984552524 1108 10-11 0.174739336174504 1108 12-13 0.22151436209281172 1108 14-15 0.21992801330046774 1108 16-17 0.2556010428915556 1108 18-19 0.19855812863458766 1108 20-21 0.24679738442287658 1108 22-23 0.341189212244565 1108 24-25 0.2912146781483784 1108 26-27 0.1847588224718848 1108 28-29 0.34886874498211284 1108 30-31 0.37608256942074547 1108 32-33 0.2714160610613945 1108 34-35 0.29561474929887055 1108 36-37 0.3808397788221356 1108 38-39 0.35280101174937784 1108 40-41 0.27084594608786716 1108 42-43 0.3518930425628852 1108 44-45 0.2527023441915084 1108 46-47 0.2686834118649202 1108 48-49 0.29202964557345723 1108 50-51 0.14298986933770763 1108 52-53 0.28632697053132006 1108 54-55 0.3199034228230282 1108 56-57 0.3425744048931598 1108 58-59 0.24027197992656824 1108 60-61 0.29845712489525766 1108 62-63 0.21433659498560687 1108 64-65 0.28536466975330654 1108 66-67 0.28274738434203783 1108 68-69 0.1873739191331616 1108 70-71 0.19374372125836814 1108 72-73 0.15494129714504368 1108 74-75 0.2595537464733191 1108 76-77 0.23760209563677392 1109 1 -0.17316228969072966 1109 2 -0.214491133913711 1109 3 -0.09356479919467375 1109 4 0.1265365987924909 1109 5 0.004146614431959961 1109 6 -0.1258900300626138 1109 7 -0.12158309429003111 1109 8 0.003791915393009049 1109 9 -0.057034695406073865 1109 10-11 -0.052977735917188795 1109 12-13 0.09351815811900366 1109 14-15 0.2320495577801509 1109 16-17 0.20723063650884654 1109 18-19 0.09607785426405258 1109 20-21 0.12976027038262572 1109 22-23 0.057295340953338325 1109 24-25 0.043666668197069214 1109 26-27 -0.09329387936556799 1109 28-29 0.1490111913633072 1109 30-31 0.37314399733001125 1109 32-33 0.32722711206690747 1109 34-35 0.34664146925859285 1109 36-37 0.3764220268665568 1109 38-39 0.34561902203365236 1109 40-41 0.3098140234870286 1109 42-43 0.3868230875660643 1109 44-45 0.24230844831191334 1109 46-47 0.24411273438847658 1109 48-49 0.1669615813270866 1109 50-51 0.14996865877365906 1109 52-53 0.35280835965680524 1109 54-55 0.38453492928644906 1109 56-57 0.3251711610621193 1109 58-59 0.4768100921808909 1109 60-61 0.49647895186118873 1109 62-63 0.26672693869166153 1109 64-65 0.35435871973645305 1109 66-67 0.4860309999246546 1109 68-69 0.37250488406768056 1109 70-71 0.2613052446591446 1109 72-73 0.16882034093985965 1109 74-75 0.29960106570370115 1109 76-77 0.3074070387551622 1110 1 -0.5702787181832321 1110 2 -0.4519394071997169 1110 3 -0.5696703084708119 1110 4 -0.4621668426681751 1110 5 -0.44095653201878093 1110 6 -0.5899696460484734 1110 7 -0.5791052687489113 1110 8 -0.8222372482840754 1110 9 -0.5985850539728759 1110 10-11 -0.7353018006363854 1110 12-13 -0.5847127368497596 1110 14-15 -0.5320576202050731 1110 16-17 -0.6142770881956423 1110 18-19 -0.6143206579515805 1110 20-21 -0.6532582044309407 1110 22-23 -0.5175436713689763 1110 24-25 -0.4520044730825461 1110 26-27 -0.5523272272263569 1110 28-29 -0.4544861847307615 1110 30-31 -0.334269730824019 1110 32-33 -0.2936085123538774 1110 34-35 -0.32291330162636456 1110 36-37 -0.3277551299378345 1110 38-39 -0.3275248643737356 1110 40-41 -0.31301791229045506 1110 42-43 -0.43679744395902276 1110 44-45 -0.4197763132600052 1110 46-47 -0.4595276019650427 1110 48-49 -0.5451021025400777 1110 50-51 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22-23 -0.7083862775840899 2314 24-25 -0.8094876588166287 2314 26-27 -0.756692652598332 2314 28-29 -0.9471813592662457 2314 30-31 -1.0192267078031065 2314 32-33 -1.151031586860121 2314 34-35 -1.1538802952392544 2314 36-37 -1.5232823093009102 2314 38-39 -1.1262173477957589 2314 40-41 -0.9999179749723197 2314 42-43 -0.878201252751829 2314 44-45 -0.8986390503747792 2314 46-47 -0.7745367134250216 2314 48-49 -0.7667894026345436 2314 50-51 -0.633045270700535 2314 52-53 -0.4276885022593575 2314 54-55 -0.5134917149548563 2314 56-57 -0.507673301563095 2314 58-59 -0.5183525921913841 2314 60-61 -0.5304740456023751 2314 62-63 -0.5038027518242245 2314 64-65 -0.4094916029269058 2314 66-67 -0.5073368406510141 2314 68-69 -0.4701663741482953 2314 70-71 -0.41565313097978773 2314 72-73 -0.2534491893064015 2314 74-75 -0.2514499058175055 2314 76-77 -0.16874721817928773 2315 1 -1.5893840989094983 2315 2 -1.4351931519189023 2315 3 -1.2550777099463843 2315 4 -1.3000520365825707 2315 5 -1.5087707277589786 2315 6 -1.5376896267251894 2315 7 -1.9249692832786565 2315 8 -1.8277356262258806 2315 9 -1.3879427318382511 2315 10-11 -1.5682808321250263 2315 12-13 -1.5119295332356089 2315 14-15 -1.480981140678992 2315 16-17 -1.4131346079320863 2315 18-19 -1.433058660953371 2315 20-21 -1.3202608191556493 2315 22-23 -1.5571714232524414 2315 24-25 -1.7282433849222016 2315 26-27 -1.5823141385032713 2315 28-29 -1.5938480227877747 2315 30-31 -1.5957225717975447 2315 32-33 -1.51232433981599 2315 34-35 -1.609910786662951 2315 36-37 -1.7292134931882757 2315 38-39 -1.5899458480679698 2315 40-41 -1.7339434731847874 2315 42-43 -1.8280826026549768 2315 44-45 -1.766344852859877 2315 46-47 -1.6171477609520508 2315 48-49 -1.615918326933425 2315 50-51 -1.5654510889427407 2315 52-53 -1.634745517522994 2315 54-55 -1.7932334021356446 2315 56-57 -1.8682858096893291 2315 58-59 -1.7047148132457153 2315 60-61 -1.7340395871610497 2315 62-63 -1.5706298831149184 2315 64-65 -1.4634046029203027 2315 66-67 -1.5608899274877928 2315 68-69 -1.600151211467928 2315 70-71 -1.5989436118924552 2315 72-73 -1.5334777596836027 2315 74-75 -1.5161620460576692 2315 76-77 -1.6668637245543145 2316 1 -2.575692726600856 2316 2 -2.2808837980211436 2316 3 -2.579338895217184 2316 4 -3.529719197554286 2316 5 -2.9856305243794026 2316 6 -2.7638762826617196 2316 7 -2.4906940961802277 2316 8 -2.3241541083040786 2316 9 -2.646450899988878 2316 10-11 -2.5108171393111647 2316 12-13 -3.084384760605481 2316 14-15 -4.3731919816285085 2316 16-17 -3.447646125490362 2316 18-19 -2.887896081391361 2316 20-21 -2.6909940431819663 2316 22-23 -3.167730027649206 2316 24-25 -3.0905218721231265 2316 26-27 -2.869076300475708 2316 28-29 -3.499765017145677 2316 30-31 -3.667495204847288 2316 32-33 -3.521767634503277 2316 34-35 -3.458182588837307 2316 36-37 -3.4420048166513837 2316 38-39 -3.5645928148191643 2316 40-41 -3.3669721173891958 2316 42-43 -3.607573717072597 2316 44-45 -3.480696182991718 2316 46-47 -3.4201942552930333 2316 48-49 -3.1615315994626414 2316 50-51 -3.2271669228746305 2316 52-53 -3.2244679942795855 2316 54-55 -3.345974820845445 2316 56-57 -3.1852860800319895 2316 58-59 -3.2855135274676144 2316 60-61 -3.1025098404850695 2316 62-63 -3.0441272145779976 2316 64-65 -3.168373695275122 2316 66-67 -3.2763638899799403 2316 68-69 -3.4941606458835075 2316 70-71 -3.2896776172800877 2316 72-73 -3.200792078777358 2316 74-75 -3.2280810234855224 2316 76-77 -3.5568657051172217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 121596.0 3 9955.0 4 13339.0 5 16279.0 6 18156.0 7 19147.0 8 22520.0 9 23864.0 10 28993.0 11 32873.0 12 25488.0 13 20806.0 14 20367.0 15 20831.0 16 21649.0 17 22470.0 18 23838.0 19 25109.0 20 26340.0 21 28438.0 22 31180.0 23 35487.0 24 40790.0 25 47810.0 26 55811.0 27 65571.0 28 76966.0 29 89593.0 30 105614.0 31 126556.0 32 153994.0 33 192002.0 34 251268.0 35 332822.0 36 466219.0 37 627847.0 38 563272.0 39 148547.0 40 1050.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.180723409528287 26.758515348997175 29.278714725376993 21.782046516097544 2 21.879730813099215 26.97511209798363 29.297595172642275 21.847561916274884 3 22.15174506828528 27.084699038947903 28.800101163378855 21.963454729387962 4 21.821079902440975 27.036714133488328 29.115875751098525 22.026330212972173 5 22.15023141708187 27.147348187865145 28.98062672298237 21.721793672070614 6 22.150569180536834 27.02141919558515 29.056457752160508 21.771553871717504 7 22.141939034574996 26.86809743177545 29.023325008814343 21.966638524835208 8 21.815261210762614 27.170846313714947 28.89764912716445 22.11624334835799 9 21.92421031439211 26.867913227848856 29.099360431103882 22.108516026655153 10-11 22.282071831250068 27.035014930012757 28.906505403221633 21.776407835515535 12-13 22.238756931594107 26.864853011253437 28.82690768700002 22.06948237015244 14-15 22.035557791178874 27.084336234206326 28.94989308080906 21.93021289380574 16-17 22.34287228036323 26.9177102227932 28.80563902810347 21.9337784687401 18-19 22.123738367487743 27.002046533083934 28.634455099185534 22.239760000242793 20-21 22.224922699197833 27.183643337411866 28.530584208431186 22.060849754959115 22-23 22.31051357883746 27.24721960630432 28.159460116697687 22.282806698160535 24-25 22.303400662819087 27.451080574696146 27.91371337406026 22.331805388424506 26-27 22.394346480643986 27.49193744496768 27.87300168579259 22.24071438859574 28-29 22.506116060426308 27.481877531603914 27.757373698468076 22.254632709501703 30-31 22.316815074430227 27.39776884452357 27.87482586287105 22.410590218175155 32-33 22.28934980813484 27.66258590179913 27.746208409088435 22.30185588097759 34-35 22.35202282440361 27.409782490626295 27.886728257250514 22.351466427719583 36-37 22.36185902238644 27.415920441418866 28.076278414569302 22.14594212162539 38-39 22.33281353757079 27.432342559866196 28.046752026817767 22.18809187574525 40-41 22.271501863129558 27.37319028200929 27.982022099080023 22.37328575578113 42-43 22.16759327500363 27.609383370014918 27.77418493284766 22.44883842213379 44-45 22.34220662916729 27.381644459715442 27.890755483174285 22.385393427942983 46-47 22.141625956833877 27.38382996589353 27.955091247775872 22.519452829496725 48-49 22.251415395389472 27.378554074230692 27.85626859146867 22.513761938911166 50-51 22.115162069972946 27.500584941765094 27.781483462905836 22.60276952535612 52-53 22.11794230768501 27.39025934841826 27.951223257235824 22.540575086660905 54-55 22.125493046881758 27.370710043156937 27.887874929365992 22.615921980595314 56-57 22.013072364895166 27.545192176612236 27.765269534378884 22.676465924113714 58-59 22.09623932624808 27.58310076168506 27.730022728770138 22.590637183296725 60-61 22.054499373178675 27.37692252923448 27.782304504526607 22.786273593060237 62-63 21.971688909360548 27.413200122526423 27.821414957582434 22.793696010530596 64-65 22.054370463373882 27.28561983420906 27.788369948407222 22.871639754009834 66-67 21.99922199124209 27.328847558086977 27.919663766670215 22.752266684000716 68-69 22.037064299829105 27.127201835837067 28.047536078792056 22.788197785541772 70-71 21.79007687427296 27.099152188035497 28.13880509307082 22.97196584462073 72-73 21.91603912451892 26.927675614588114 28.05454384883014 23.10174141206283 74-75 21.791685900784348 26.84379264406354 27.99605548739254 23.36846596775957 76-77 21.710026496736624 26.76603153794955 28.358543615855524 23.165398349458297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11884.0 1 7288.5 2 2499.5 3 2493.5 4 2681.0 5 2722.5 6 2920.5 7 3219.0 8 3449.5 9 3538.0 10 3652.5 11 3906.5 12 4298.0 13 4550.0 14 4684.0 15 5215.5 16 6072.0 17 6531.0 18 7026.5 19 8278.5 20 10145.0 21 12859.0 22 14463.0 23 17026.0 24 22394.5 25 29589.5 26 33979.0 27 38693.5 28 50166.0 29 61621.0 30 66318.0 31 74692.0 32 91215.5 33 106093.5 34 118784.5 35 124747.0 36 133224.0 37 148445.0 38 157222.5 39 159256.0 40 162997.0 41 170135.5 42 178063.5 43 182594.0 44 182860.0 45 184899.0 46 187343.0 47 189256.5 48 190499.0 49 187937.0 50 183048.0 51 174513.0 52 168305.0 53 163292.5 54 150587.0 55 133031.5 56 114831.0 57 106493.0 58 100427.5 59 85374.0 60 70080.0 61 63774.0 62 55216.0 63 39371.5 64 27478.0 65 22871.0 66 19572.0 67 14146.0 68 10188.0 69 7108.5 70 5860.0 71 4850.0 72 3283.5 73 2465.5 74 2204.0 75 1963.0 76 1361.5 77 805.0 78 609.0 79 491.5 80 340.5 81 264.0 82 191.0 83 161.0 84 132.0 85 90.5 86 74.0 87 70.0 88 71.5 89 60.5 90 50.5 91 53.0 92 50.0 93 40.5 94 34.5 95 34.0 96 33.0 97 32.0 98 34.0 99 80.0 100 123.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032115660885932 2 0.008269150480078554 3 0.011556580334544035 4 0.018915365624155227 5 0.014085372530286711 6 0.013326734871563908 7 0.01706934732126307 8 0.01856133471675125 9 0.01770154537019874 10-11 0.02133036217108948 12-13 0.02076138392704738 14-15 0.007649596392121599 16-17 0.005803578089229444 18-19 0.011455428646714328 20-21 0.010696790987991525 22-23 0.01430031986692484 24-25 0.022493606581131115 26-27 0.013415242598414904 28-29 0.008054203143440426 30-31 0.008028915221483 32-33 0.009558834499907319 34-35 0.011215193388118773 36-37 0.009849645602417727 38-39 0.016702672452880384 40-41 0.012429013642075258 42-43 0.015450920315987758 44-45 0.017764765175092307 46-47 0.016740604335816523 48-49 0.013314090910585195 50-51 0.027108652338361502 52-53 0.03190071354929387 54-55 0.02475687559632081 56-57 0.015678511613604597 58-59 0.016626808687008103 60-61 0.019686647243856742 62-63 0.025894832084405017 64-65 0.020306201331813697 66-67 0.019572851595048322 68-69 0.029460429080402188 70-71 0.05625298239429585 72-73 0.06736702409458492 74-75 0.0795305145561072 76-77 0.07654653976513083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3954457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.39113121266262 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58859959181518 38.02998845758218 2 13.935729188037064 14.602172328691642 3 5.5056494297598135 8.653416050564028 4 2.669874855908274 5.595110555891165 5 1.4967762604156005 3.920890072771611 6 0.9158924415462573 2.879078464904154 7 0.6226101685000879 2.2833475722558267 8 0.41923778728061484 1.757147353818003 9 0.29584964943266584 1.3949908022382331 >10 1.4251867211336933 12.998543151308306 >50 0.08233949779553604 2.959319881289405 >100 0.04036629650438506 3.8012459925352653 >500 0.0014039806617558081 0.4741326915249103 >1k 4.3571808808008433E-4 0.3588017142391425 >5k 0.0 0.0 >10k+ 4.841312089778578E-5 0.29181491038606383 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11505 0.290937542120195 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4202 0.10625984806510728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.586376587228031E-5 2.528792195742677E-5 2 0.0 0.0 0.0 3.0345506348912124E-4 2.528792195742677E-5 3 0.0 0.0 0.0 5.563342830633889E-4 2.528792195742677E-5 4 5.057584391485354E-5 0.0 0.0 0.001087380644169351 2.528792195742677E-5 5 7.586376587228031E-5 0.0 0.0 0.002200049210296129 2.528792195742677E-5 6 7.586376587228031E-5 0.0 0.0 0.0037426124496991622 2.528792195742677E-5 7 7.586376587228031E-5 0.0 0.0 0.007636952431142885 2.528792195742677E-5 8 7.586376587228031E-5 0.0 0.0 0.011657732022373742 2.528792195742677E-5 9 1.2643960978713385E-4 0.0 0.0 0.01620955797471056 2.528792195742677E-5 10 1.2643960978713385E-4 0.0 0.0 0.022126931712748425 2.528792195742677E-5 11 2.2759129761684094E-4 0.0 0.0 0.02834776051427541 2.528792195742677E-5 12 2.7816714153169447E-4 0.0 0.0 0.03684450229197081 2.528792195742677E-5 13 2.7816714153169447E-4 0.0 0.0 0.049311447816982205 2.528792195742677E-5 14 2.7816714153169447E-4 0.0 0.0 0.06382671502054517 2.528792195742677E-5 15 3.0345506348912124E-4 0.0 0.0 0.08210988259576472 2.528792195742677E-5 16 3.0345506348912124E-4 0.0 0.0 0.100494201858814 2.528792195742677E-5 17 3.0345506348912124E-4 0.0 0.0 0.11743710957028992 2.528792195742677E-5 18 3.0345506348912124E-4 0.0 0.0 0.1359984442870412 2.528792195742677E-5 19 3.540309074039748E-4 0.0 0.0 0.15584946302362118 2.528792195742677E-5 20 3.7931882936140157E-4 0.0 0.0 0.176813150326328 2.528792195742677E-5 21 3.7931882936140157E-4 0.0 0.0 0.1993952646343101 2.528792195742677E-5 22 4.298946732762551E-4 0.0 0.0 0.22539124840654481 2.528792195742677E-5 23 4.298946732762551E-4 0.0 0.0 0.25227230944728946 2.528792195742677E-5 24 4.298946732762551E-4 0.0 0.0 0.27869818789280043 2.528792195742677E-5 25 4.298946732762551E-4 0.0 0.0 0.3078298739877561 2.528792195742677E-5 26 4.298946732762551E-4 0.0 0.0 0.3388075783856039 2.528792195742677E-5 27 4.298946732762551E-4 0.0 0.0 0.3702657533006428 2.528792195742677E-5 28 4.298946732762551E-4 0.0 0.0 0.4021285349670005 2.528792195742677E-5 29 4.298946732762551E-4 0.0 0.0 0.43533157649710186 2.528792195742677E-5 30 4.551825952336819E-4 0.0 0.0 0.47204963917928555 2.528792195742677E-5 31 4.551825952336819E-4 0.0 0.0 0.5106390080863188 2.528792195742677E-5 32 4.8047051719110866E-4 0.0 0.0 0.5519341846427968 2.528792195742677E-5 33 4.8047051719110866E-4 0.0 0.0 0.5979329146833561 2.528792195742677E-5 34 4.8047051719110866E-4 0.0 0.0 0.6474466658759976 2.528792195742677E-5 35 4.8047051719110866E-4 0.0 0.0 0.6988570112154463 2.528792195742677E-5 36 4.8047051719110866E-4 0.0 0.0 0.7539846810826366 2.528792195742677E-5 37 5.057584391485354E-4 0.0 0.0 0.8095675335450607 2.528792195742677E-5 38 5.057584391485354E-4 0.0 0.0 0.8634308073143797 2.528792195742677E-5 39 5.057584391485354E-4 0.0 0.0 0.9205815109381642 2.528792195742677E-5 40 5.057584391485354E-4 2.528792195742677E-5 0.0 0.9804633101333508 2.528792195742677E-5 41 5.057584391485354E-4 2.528792195742677E-5 0.0 1.0478556221498931 2.528792195742677E-5 42 6.069101269782425E-4 2.528792195742677E-5 0.0 1.1175238471426039 2.528792195742677E-5 43 6.069101269782425E-4 2.528792195742677E-5 0.0 1.1951830554738616 2.528792195742677E-5 44 6.827738928505228E-4 2.528792195742677E-5 0.0 1.2778239844307322 2.528792195742677E-5 45 6.827738928505228E-4 2.528792195742677E-5 0.0 1.365269618559514 2.528792195742677E-5 46 6.827738928505228E-4 2.528792195742677E-5 0.0 1.4623752388760327 2.528792195742677E-5 47 7.080618148079496E-4 2.528792195742677E-5 0.0 1.559506147114509 2.528792195742677E-5 48 7.080618148079496E-4 2.528792195742677E-5 0.0 1.6566370553529852 2.528792195742677E-5 49 7.586376587228031E-4 2.528792195742677E-5 0.0 1.7578140311046497 2.528792195742677E-5 50 7.586376587228031E-4 2.528792195742677E-5 0.0 1.86129220775444 2.528792195742677E-5 51 7.586376587228031E-4 2.528792195742677E-5 0.0 1.9715222595668633 2.528792195742677E-5 52 7.586376587228031E-4 2.528792195742677E-5 0.0 2.0895157034202168 2.528792195742677E-5 53 7.586376587228031E-4 2.528792195742677E-5 0.0 2.2136541123092246 2.528792195742677E-5 54 7.586376587228031E-4 2.528792195742677E-5 0.0 2.3520801971041787 2.528792195742677E-5 55 7.586376587228031E-4 2.528792195742677E-5 0.0 2.496448943559128 2.528792195742677E-5 56 8.85077268509937E-4 2.528792195742677E-5 0.0 2.6492891438698156 2.528792195742677E-5 57 8.85077268509937E-4 2.528792195742677E-5 0.0 2.805315622347139 2.528792195742677E-5 58 8.85077268509937E-4 2.528792195742677E-5 0.0 2.968321567284712 2.528792195742677E-5 59 8.85077268509937E-4 2.528792195742677E-5 0.0 3.131757406895561 2.528792195742677E-5 60 8.85077268509937E-4 2.528792195742677E-5 0.0 3.298505964282833 2.528792195742677E-5 61 9.86228956339644E-4 2.528792195742677E-5 0.0 3.472891474101248 2.528792195742677E-5 62 9.86228956339644E-4 2.528792195742677E-5 0.0 3.6570634097171877 2.528792195742677E-5 63 0.0010115168782970708 2.528792195742677E-5 0.0 3.852539046448096 2.528792195742677E-5 64 0.0010115168782970708 2.528792195742677E-5 0.0 4.0589137775426565 2.528792195742677E-5 65 0.0010115168782970708 2.528792195742677E-5 0.0 4.281826809597374 2.528792195742677E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 175 4.461981E-9 22.316628 59 TGCGTCG 115 3.683273E-5 21.618073 67 ATTCGCG 180 1.4693069E-7 19.721548 56 ACGCTCG 205 2.7584974E-8 19.049816 45 GCGTTCG 95 0.0066504763 18.68239 10 TAACGCG 210 3.63234E-8 18.597897 52 CGCACGA 210 3.645073E-8 18.59225 4 TCGAACT 1230 0.0 18.470444 24 CGAACTC 1220 0.0 17.456654 25 CTCGAAC 1245 0.0 17.39276 23 GCCTCGA 1260 0.0 16.90397 21 TTAGTCG 390 0.0 16.383018 15 GTAGACC 2155 0.0 16.141666 8 GTAACGT 220 1.2076416E-6 16.132751 2 CGATCGT 265 2.9491275E-8 16.071875 1 AACGCGA 245 2.1097912E-7 15.940851 53 ACGCGAA 245 2.1122287E-7 15.9392395 54 CGTTCGG 140 0.0034202624 15.21261 11 GCGTAAG 305 1.0075382E-8 15.130439 38 CGCGAAT 275 7.8186895E-7 14.200053 55 >>END_MODULE