FastQCFastQC Report
Thu 26 May 2016
SRR936078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3954457
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140570.3554723189555481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103850.262615069527877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73550.1859926659968739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA21050.048.91527100-101
CGGTATC15500.042.238792100-101
CGTACAT28300.040.27475100-101
GGTATCA137750.039.959331
TACGCAG16900.038.211452100-101
CGAGTAC15600.035.482063100-101
ACGTATC7350.033.20068100-101
CCGTACA12650.032.22928100-101
CCGTATC10000.031.842289100-101
GCGTATC5400.030.310242100-101
GTATCAA272550.030.2174641
TCGTATC10500.030.042568100-101
CCGGTAT7300.029.759148100-101
AGTATCA51250.029.613981100-101
CCTGTCG11100.029.48713396-97
TCGTACA12800.028.829174100-101
TCGGTAT6650.028.640383100-101
AGGTATC34700.027.443596100-101
TTACGCA7650.026.841583100-101
GCGTACA10250.026.129984100-101
Produced by FastQC (version 0.11.5)