FastQCFastQC Report
Thu 26 May 2016
SRR936077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306884
Sequences flagged as poor quality0
Sequence length77
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207131.584914958022288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138901.0628334266851533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG500.00673536828.3938624
CGTAGGC3100.022.8982754
TACGTAG3150.022.53742
CGCCGGA800.002446999822.20224471
GGGTCGC850.003479265120.89543269
ACGTAGG3850.020.282883
ATACGTA3600.019.7202241
CGTGTGA1309.497029E-519.11271714
CAACCGG950.006645361418.68374660
ACGAAGC2507.185008E-1018.45742242
CTATTCG1551.759532E-518.3193239
GGACGAA3350.018.013137
TTACGAG1602.3052819E-517.75295651
CGAGCCG1803.1945965E-617.7509254
GCCCTTA1000.00893619817.74820126
CAACGAA3002.3646862E-1117.748240
TTACGGC4300.017.33677958
CGAACAT3500.017.2411140
AGACGTC3750.017.0506768
TAGTCGG4200.016.9030516
Produced by FastQC (version 0.11.5)