##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936077_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1306884 Sequences flagged as poor quality 0 Sequence length 77 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.73656116380643 40.0 36.0 41.0 30.0 41.0 2 36.57760137854622 39.0 36.0 41.0 30.0 41.0 3 36.482564634657706 39.0 36.0 41.0 30.0 41.0 4 36.358895663272335 39.0 36.0 41.0 30.0 41.0 5 36.26765573685193 39.0 35.0 41.0 30.0 41.0 6 36.13516731400798 39.0 35.0 40.0 30.0 41.0 7 35.917885596579346 39.0 35.0 40.0 29.0 41.0 8 35.648094245548954 38.0 35.0 40.0 28.0 41.0 9 35.50776503499928 38.0 35.0 40.0 28.0 41.0 10-11 35.65181798843662 38.0 35.0 40.0 28.5 41.0 12-13 35.718879793462925 39.0 35.0 41.0 29.0 41.0 14-15 35.59198253249714 39.0 35.0 41.0 27.5 41.0 16-17 35.35383094444495 39.0 35.0 41.0 26.5 41.0 18-19 35.10034478959112 39.0 35.0 41.0 25.5 41.0 20-21 34.739377787163974 39.0 34.5 41.0 23.5 41.0 22-23 34.2847884739579 38.0 34.0 41.0 21.0 41.0 24-25 33.91309213365532 38.0 33.5 40.5 16.0 41.0 26-27 32.94773101514748 37.5 32.0 39.5 10.5 40.5 28-29 32.97062937491009 38.0 33.0 39.5 8.5 40.5 30-31 33.33510548755666 38.0 33.0 40.0 7.5 41.0 32-33 33.17696482625849 38.0 33.0 40.0 2.0 41.0 34-35 32.91771687464228 38.0 33.0 40.0 2.0 41.0 36-37 32.64368987607163 37.5 32.5 40.0 2.0 41.0 38-39 32.36376908738649 37.0 32.0 40.0 2.0 41.0 40-41 31.97693521383688 36.5 31.5 40.0 2.0 41.0 42-43 31.609386525506473 36.0 31.0 39.0 2.0 41.0 44-45 31.225501651255964 35.5 31.0 39.0 2.0 41.0 46-47 30.817301688596693 35.0 30.5 39.0 2.0 40.0 48-49 30.321368231610457 35.0 30.0 38.0 2.0 40.0 50-51 29.8551868413723 35.0 29.5 37.0 2.0 39.5 52-53 29.369646808745074 35.0 29.0 37.0 2.0 39.0 54-55 28.8652011196097 34.5 29.0 36.0 2.0 39.0 56-57 28.355336433838044 34.0 28.0 36.0 2.0 37.5 58-59 27.872190263252133 34.0 26.5 35.0 2.0 37.0 60-61 27.454589695795494 34.0 26.0 35.0 2.0 37.0 62-63 27.066476443203833 34.0 26.0 35.0 2.0 36.0 64-65 26.632880194416643 34.0 25.0 35.0 2.0 36.0 66-67 26.19353668726528 33.0 24.0 35.0 2.0 35.5 68-69 25.767067314314048 33.0 21.5 35.0 2.0 35.0 70-71 25.302655782762663 33.0 18.5 35.0 2.0 35.0 72-73 24.786397262496138 33.0 6.5 35.0 2.0 35.0 74-75 24.20153357145699 32.0 2.0 35.0 2.0 35.0 76-77 22.580226324601114 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1390160616840745 1101 2 0.967610912792793 1101 3 1.243741666761423 1101 4 1.1103663999960744 1101 5 1.0229567031875746 1101 6 1.0340186488436274 1101 7 1.0350405437809655 1101 8 1.1337509514169781 1101 9 1.184777360717284 1101 10-11 1.0924425014987449 1101 12-13 1.184586555478127 1101 14-15 1.257919399815293 1101 16-17 1.4095106864287459 1101 18-19 1.2184043003091247 1101 20-21 1.1060128674133765 1101 22-23 1.3245980919004694 1101 24-25 1.3171755478054408 1101 26-27 1.0634272639496913 1101 28-29 1.0687291679741335 1101 30-31 1.227096340851773 1101 32-33 1.3494478977616424 1101 34-35 1.2142070721455056 1101 36-37 1.2732365337157034 1101 38-39 1.3521639825032636 1101 40-41 1.1974763576518939 1101 42-43 1.047745078930145 1101 44-45 1.0006920918187916 1101 46-47 1.2278449576762078 1101 48-49 1.3170159380467688 1101 50-51 1.1641860266031472 1101 52-53 1.3313936715072323 1101 54-55 1.2299055069111517 1101 56-57 1.177879303820049 1101 58-59 1.1161118599362574 1101 60-61 1.1689949109113833 1101 62-63 1.1427649372243351 1101 64-65 1.3165644046633638 1101 66-67 1.3415871800391201 1101 68-69 1.3164566667971265 1101 70-71 1.3002961335926138 1101 72-73 1.3074036270871296 1101 74-75 1.350903463928102 1101 76-77 1.4457889976431346 1102 1 0.9822552226361196 1102 2 0.9966164097848704 1102 3 1.1640972561387102 1102 4 1.2956282926101608 1102 5 1.245716037552853 1102 6 1.400511210464991 1102 7 1.1643553366564987 1102 8 1.295579284995327 1102 9 1.3259624280388635 1102 10-11 1.1991848923299742 1102 12-13 1.3003455122314662 1102 14-15 1.2106811555090147 1102 16-17 1.284709941581994 1102 18-19 1.3469552217110632 1102 20-21 1.3591101500487142 1102 22-23 1.4778040460624098 1102 24-25 1.6568965761782195 1102 26-27 1.6271139671092385 1102 28-29 1.53189687091173 1102 30-31 1.657657541369474 1102 32-33 1.6363782009753862 1102 34-35 1.7013329552818206 1102 36-37 1.6691698549423961 1102 38-39 1.7321893199759089 1102 40-41 1.7817076002834114 1102 42-43 1.7603391576290797 1102 44-45 1.8100600386187438 1102 46-47 1.4305324881229744 1102 48-49 1.4632721030660711 1102 50-51 1.6094121181466576 1102 52-53 1.5338001549023978 1102 54-55 1.4693972297151632 1102 56-57 1.6881310355022663 1102 58-59 1.780173436624633 1102 60-61 1.6912855452706097 1102 62-63 1.6434408914151035 1102 64-65 1.6454658421830004 1102 66-67 1.6116690728261567 1102 68-69 1.587493398926135 1102 70-71 1.7142272756237027 1102 72-73 1.4789345696468743 1102 74-75 1.4706864379535922 1102 76-77 1.4019989844699552 1103 1 0.5791322825883398 1103 2 0.3814816128095657 1103 3 0.33318653158134737 1103 4 0.27613196011235175 1103 5 0.4383365277007698 1103 6 0.5262433463494105 1103 7 0.5696510670709429 1103 8 0.4659302483706895 1103 9 0.41490827832415533 1103 10-11 0.4905434846137382 1103 12-13 0.6489629037913929 1103 14-15 0.6064774884266839 1103 16-17 0.6828764004165393 1103 18-19 0.649871589225441 1103 20-21 0.7184130517210221 1103 22-23 0.5291711195762048 1103 24-25 0.23593681163730906 1103 26-27 0.19439489315878689 1103 28-29 0.23950541596092023 1103 30-31 0.4258470506757277 1103 32-33 0.46010822075091795 1103 34-35 0.4404165205893875 1103 36-37 0.3571655555465725 1103 38-39 0.3992223169990794 1103 40-41 0.40923674926335707 1103 42-43 0.3521306285346846 1103 44-45 0.30409903548236983 1103 46-47 -0.07905298126272342 1103 48-49 0.00757813271678387 1103 50-51 0.20740391402612346 1103 52-53 0.0924623458308389 1103 54-55 0.23014047097256807 1103 56-57 0.2495412289330936 1103 58-59 0.10615812002703251 1103 60-61 0.176662771068667 1103 62-63 0.1749159116734873 1103 64-65 0.2309205326810826 1103 66-67 0.2197186838232028 1103 68-69 0.15809124167069655 1103 70-71 0.17134982939764498 1103 72-73 0.17426002635271942 1103 74-75 0.11102770249658889 1103 76-77 0.48356889373758705 1104 1 0.4299142310767934 1104 2 0.5647957193009958 1104 3 0.5291288824742892 1104 4 0.34604389912740885 1104 5 0.37598691882869417 1104 6 0.3921096573024272 1104 7 0.5200171283823707 1104 8 0.6753901277914096 1104 9 0.7625630475713194 1104 10-11 0.6041860729906219 1104 12-13 0.9191140342818827 1104 14-15 0.8892759877084941 1104 16-17 0.7578761101356264 1104 18-19 0.7798252330705608 1104 20-21 0.7984629663784304 1104 22-23 0.7940251355956107 1104 24-25 0.6472085220885901 1104 26-27 0.45414508764699946 1104 28-29 0.6258799029320841 1104 30-31 0.7370223878626305 1104 32-33 0.7244076294657731 1104 34-35 0.6230079983514827 1104 36-37 0.7306836714663305 1104 38-39 0.7681970243105454 1104 40-41 0.8965132244197491 1104 42-43 0.8339330886142342 1104 44-45 0.8617467765162772 1104 46-47 0.6751856323829379 1104 48-49 0.6869444187397917 1104 50-51 0.46481751788761017 1104 52-53 0.4813260770402721 1104 54-55 0.4903752638383523 1104 56-57 0.5770689439305059 1104 58-59 0.49994433257139903 1104 60-61 0.45706867258215667 1104 62-63 0.5996768055747879 1104 64-65 0.6748607990155335 1104 66-67 0.5116699111650682 1104 68-69 0.5165000651177465 1104 70-71 0.5779968525839152 1104 72-73 0.6572936190264365 1104 74-75 0.4745707415943592 1104 76-77 0.5312439484982292 1105 1 0.14128287410742502 1105 2 0.11595810965865638 1105 3 0.19452103960073686 1105 4 0.22263188063244854 1105 5 0.19786333937111777 1105 6 0.056665963905579986 1105 7 0.2345422334856977 1105 8 0.2743432636766343 1105 9 0.18452903793651387 1105 10-11 0.45870403671617765 1105 12-13 0.4917590607359088 1105 14-15 0.17158769607130608 1105 16-17 0.33339281773184837 1105 18-19 0.39950652050900004 1105 20-21 0.3527243246657932 1105 22-23 0.38321267274826454 1105 24-25 0.3936936462355902 1105 26-27 0.15106925186372422 1105 28-29 0.21531590892492858 1105 30-31 0.2903964534987509 1105 32-33 0.5178301650179975 1105 34-35 0.4665758495095744 1105 36-37 0.41937171564335074 1105 38-39 0.5222838155859577 1105 40-41 0.4812010883812121 1105 42-43 0.3685364933434023 1105 44-45 0.32747724128413225 1105 46-47 0.18042432385659168 1105 48-49 0.24537897762858663 1105 50-51 0.40766027039785513 1105 52-53 0.19888156056937945 1105 54-55 0.08406650885888922 1105 56-57 0.1331264057960908 1105 58-59 0.21064792639242924 1105 60-61 -0.026570377170788362 1105 62-63 0.13229514936690023 1105 64-65 0.3681850229454682 1105 66-67 0.4554702269457671 1105 68-69 0.16323318739265602 1105 70-71 -0.28710238701401636 1105 72-73 -0.39735237843978766 1105 74-75 -0.11056766126542428 1105 76-77 0.34511422557292804 1106 1 -0.14486754327906226 1106 2 0.05347673709670886 1106 3 -0.22412486104413176 1106 4 -0.032674796232605274 1106 5 -0.11354192774305005 1106 6 0.023679243785309723 1106 7 0.19200969316471372 1106 8 0.20076982871111682 1106 9 0.1874823935128731 1106 10-11 0.036758277178279286 1106 12-13 0.16190966274833585 1106 14-15 0.08509835821903522 1106 16-17 0.18305488633356504 1106 18-19 0.23720779574670559 1106 20-21 0.10199670066969446 1106 22-23 0.020342596656000467 1106 24-25 0.11905465587570774 1106 26-27 -0.08070849807363345 1106 28-29 -0.1947508201628878 1106 30-31 -0.04554296281953896 1106 32-33 0.015464974919218832 1106 34-35 0.10441460513077061 1106 36-37 -0.006562623461746853 1106 38-39 -0.12201750712699067 1106 40-41 0.023563509090962498 1106 42-43 0.15862142127335588 1106 44-45 -0.2513273717267168 1106 46-47 -0.1199610827820301 1106 48-49 0.06042302410157774 1106 50-51 0.13368923029446478 1106 52-53 -0.0525726983553092 1106 54-55 -0.029878683613649315 1106 56-57 -0.0919415101727381 1106 58-59 -0.10284888881019683 1106 60-61 -0.08979058316058186 1106 62-63 -0.06515635335633263 1106 64-65 -0.06021176456326671 1106 66-67 -0.3700074926601893 1106 68-69 -0.32374672794316695 1106 70-71 -0.30853350175903316 1106 72-73 -0.2574016340016456 1106 74-75 -0.5353220428344798 1106 76-77 -0.06011662278324437 1107 1 0.30683286157923817 1107 2 0.20894232414124048 1107 3 0.1262799972554589 1107 4 0.06319564911152753 1107 5 0.12026374027314546 1107 6 0.07291001301607736 1107 7 0.2793781142173444 1107 8 0.13157954531030924 1107 9 -8.981732887463068E-4 1107 10-11 0.2230538237369828 1107 12-13 0.2947031850155426 1107 14-15 0.30712264971700876 1107 16-17 0.27750832762910704 1107 18-19 0.3021875528317324 1107 20-21 0.055174838321519815 1107 22-23 -0.038260642210396156 1107 24-25 -0.059042510116199765 1107 26-27 -0.06647772884286951 1107 28-29 0.09387266161848373 1107 30-31 0.29020602503470627 1107 32-33 0.41378481297589786 1107 34-35 0.42955630553562685 1107 36-37 0.394692437267004 1107 38-39 0.2014845171645092 1107 40-41 0.18552707184399964 1107 42-43 0.3258278990061463 1107 44-45 0.2867293084352234 1107 46-47 0.06588911964712096 1107 48-49 0.5723258581096715 1107 50-51 0.25211028292604 1107 52-53 0.3413746700657434 1107 54-55 0.349615243511856 1107 56-57 0.22564958294467274 1107 58-59 0.27294058487401074 1107 60-61 0.4370515221025748 1107 62-63 0.2553294766031833 1107 64-65 0.3075210289590018 1107 66-67 0.3408022239390007 1107 68-69 0.34117230039691293 1107 70-71 0.09535313791707978 1107 72-73 0.16302346721293048 1107 74-75 0.3890301838861667 1107 76-77 0.5423530128442913 1108 1 -0.304815909177087 1108 2 -0.13975215453034906 1108 3 -0.012659743400526224 1108 4 0.023524229760475635 1108 5 0.13187261195066924 1108 6 -0.005587904017303913 1108 7 -0.1843090300647674 1108 8 -0.11953762889611141 1108 9 -0.22620064351112745 1108 10-11 -0.27819800986039667 1108 12-13 -0.1387533660611453 1108 14-15 -0.03622417889297225 1108 16-17 0.0044302310498665065 1108 18-19 -0.03161811518446456 1108 20-21 0.07688961858321619 1108 22-23 0.2512526477033461 1108 24-25 0.048333538701498924 1108 26-27 -0.18780818709551994 1108 28-29 -0.011162250075464897 1108 30-31 0.06429098660250787 1108 32-33 0.030966940535606113 1108 34-35 0.12999108814432248 1108 36-37 0.20907429492117302 1108 38-39 0.0012413088091776103 1108 40-41 0.16147350146319184 1108 42-43 0.15045384514239046 1108 44-45 0.008051845547235814 1108 46-47 0.017080517875132983 1108 48-49 -0.03144460483699518 1108 50-51 -0.057568177438916734 1108 52-53 -0.05466739999234349 1108 54-55 0.03972173884718444 1108 56-57 -0.07048988780578469 1108 58-59 0.05171896896132111 1108 60-61 -0.1508361737256223 1108 62-63 -0.16554360912116195 1108 64-65 -0.05344652370191483 1108 66-67 -0.16227997989835075 1108 68-69 -0.17548057761752034 1108 70-71 -0.2486144734189537 1108 72-73 -0.5373054068115941 1108 74-75 -0.5188108652249035 1108 76-77 -0.2967827026987493 1109 1 0.01010983228655249 1109 2 -0.0018578663803126005 1109 3 -0.27294600893649346 1109 4 -0.01293200032881714 1109 5 0.28704154451224895 1109 6 0.11092620734806502 1109 7 -0.18571119276050752 1109 8 -0.04744403173184253 1109 9 0.00888034540618321 1109 10-11 -0.19732126556999674 1109 12-13 -9.20773069715608E-4 1109 14-15 0.11457561470867716 1109 16-17 0.32391580560005195 1109 18-19 0.1243416371375119 1109 20-21 0.022614704956431808 1109 22-23 -0.1400348741703894 1109 24-25 -0.18021898070443143 1109 26-27 -0.3190450067661814 1109 28-29 -0.23256934838389753 1109 30-31 0.06116696811639599 1109 32-33 -0.11224995720748154 1109 34-35 -5.669756034620832E-5 1109 36-37 0.1721525468168963 1109 38-39 -0.030245656686410882 1109 40-41 -0.06885135159015121 1109 42-43 -0.008198059555414972 1109 44-45 -0.09205135362717343 1109 46-47 -0.22038975627571844 1109 48-49 0.009762152465469853 1109 50-51 -0.01960322028900663 1109 52-53 -0.07787038828147885 1109 54-55 -0.15202681412091223 1109 56-57 0.09117947101326251 1109 58-59 -0.050330432034773054 1109 60-61 0.1183958898091717 1109 62-63 0.28056286550530274 1109 64-65 0.32699947620834635 1109 66-67 0.3677038671873909 1109 68-69 0.4486276469079904 1109 70-71 0.35510069685968304 1109 72-73 0.47849977109481046 1109 74-75 0.37449577093070374 1109 76-77 0.11590743056279251 1110 1 -0.10151183170216171 1110 2 -0.18697954996380162 1110 3 -0.1387609558346341 1110 4 -0.03329097669751491 1110 5 -0.015766387454675623 1110 6 -0.10458697993884414 1110 7 -0.14676656760865114 1110 8 0.00349259874979424 1110 9 -0.07603363350983727 1110 10-11 -0.017186453483937214 1110 12-13 -0.12933026478112453 1110 14-15 -0.19588897752395695 1110 16-17 -0.10770331106726161 1110 18-19 0.08028767311352425 1110 20-21 -0.09468454652297709 1110 22-23 -0.19126052765629709 1110 24-25 -0.14578115494121846 1110 26-27 -0.1136084350688833 1110 28-29 -0.07973006476905198 1110 30-31 -0.04486528489140085 1110 32-33 -0.24878148520841847 1110 34-35 0.041626298089610714 1110 36-37 0.11452919767710767 1110 38-39 0.01835919498110883 1110 40-41 0.21240232243108892 1110 42-43 0.2776750838391848 1110 44-45 0.1528442061956916 1110 46-47 0.1958229646554237 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-0.41875328114385724 2314 10-11 -0.5050861844030337 2314 12-13 -0.6720143567020003 2314 14-15 -0.6828407202463609 2314 16-17 -1.2645877244669421 2314 18-19 -0.7991097609655853 2314 20-21 -0.7085197078730303 2314 22-23 -0.4165369954867444 2314 24-25 -0.25359277466646546 2314 26-27 -0.14987821724335504 2314 28-29 -0.413255950510127 2314 30-31 -0.5116529493242652 2314 32-33 -0.5089146172235246 2314 34-35 -0.3939622129828919 2314 36-37 -0.6998374772629106 2314 38-39 -0.4491585434785499 2314 40-41 -0.3447914059744761 2314 42-43 -0.1423395816613322 2314 44-45 -0.1744530277471128 2314 46-47 -0.2936570748990768 2314 48-49 -0.2680801247963096 2314 50-51 -0.17965101133525252 2314 52-53 -0.11622301003193414 2314 54-55 -0.040615729219112495 2314 56-57 -0.1447289274338388 2314 58-59 -0.025403940970512906 2314 60-61 -0.08154534399428925 2314 62-63 -0.04803743426372975 2314 64-65 0.06089200982998122 2314 66-67 0.15623974937652818 2314 68-69 0.3770147912394073 2314 70-71 0.2949318924958355 2314 72-73 0.3759681151575833 2314 74-75 0.4691421089980956 2314 76-77 0.36338068887196684 2315 1 -0.8843979076515325 2315 2 -0.9851602399613242 2315 3 -1.0303866694112074 2315 4 -1.0088556329397562 2315 5 -1.0403516443422376 2315 6 -1.0863720382659707 2315 7 -1.26103214219291 2315 8 -1.0587794290486627 2315 9 -1.192128942519517 2315 10-11 -1.2272025865194323 2315 12-13 -1.3771942508818924 2315 14-15 -1.7303645297701635 2315 16-17 -1.528132698011916 2315 18-19 -1.4828637292195523 2315 20-21 -1.5786969565502744 2315 22-23 -1.6297221806719406 2315 24-25 -1.651709176782866 2315 26-27 -1.6448623442274837 2315 28-29 -1.4814093896635647 2315 30-31 -1.6261529493242683 2315 32-33 -1.6565998024087136 2315 34-35 -1.5401103611310418 2315 36-37 -1.9046152550406887 2315 38-39 -1.6445924059124124 2315 40-41 -1.588857543540616 2315 42-43 -1.6358131266348757 2315 44-45 -1.492860435154519 2315 46-47 -1.43557241881442 2315 48-49 -1.5844690136851973 2315 50-51 -1.3973256145098567 2315 52-53 -1.5365484068573316 2315 54-55 -1.5700257821291679 2315 56-57 -1.6217606734655838 2315 58-59 -1.3569568510234227 2315 60-61 -1.5115638625128085 2315 62-63 -1.701696164422458 2315 64-65 -1.7249148684768976 2315 66-67 -1.5818131606763828 2315 68-69 -1.4390328278082123 2315 70-71 -1.5888776313136894 2315 72-73 -2.941566276376811 2315 74-75 -3.5746621238061387 2315 76-77 -3.1248521153608415 2316 1 -3.2305070544616754 2316 2 -3.0714810188493544 2316 3 -2.6451650450197093 2316 4 -2.334541003167786 2316 5 -3.292061098608187 2316 6 -2.7966246644509596 2316 7 -3.1497249426028517 2316 8 -3.2433917785234314 2316 9 -2.620098196939189 2316 10-11 -2.7757332039931697 2316 12-13 -2.854466093054583 2316 14-15 -2.875403217958741 2316 16-17 -4.038158319328851 2316 18-19 -3.939522965704313 2316 20-21 -3.260249095930238 2316 22-23 -3.5035284835159004 2316 24-25 -3.3879664148049002 2316 26-27 -3.0906594233973514 2316 28-29 -3.067660478569536 2316 30-31 -3.325241855294035 2316 32-33 -3.3250087186270108 2316 34-35 -3.4516350857018807 2316 36-37 -3.262204032903874 2316 38-39 -3.3681834896941183 2316 40-41 -3.3273325627566024 2316 42-43 -3.585388325200082 2316 44-45 -3.317096151270327 2316 46-47 -3.088187330420876 2316 48-49 -3.3012010147100526 2316 50-51 -3.745722744922361 2316 52-53 -4.669226346903454 2316 54-55 -3.8477693127466424 2316 56-57 -3.713365080332096 2316 58-59 -3.895567663219172 2316 60-61 -3.635181080037789 2316 62-63 -3.4075357749784416 2316 64-65 -3.2789668797502785 2316 66-67 -3.5547355280860664 2316 68-69 -3.6316690051077245 2316 70-71 -3.247036739691861 2316 72-73 -3.3827197480652558 2316 74-75 -3.3032470584717792 2316 76-77 -3.3816988998855635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31885.0 3 3432.0 4 5342.0 5 6428.0 6 7119.0 7 7570.0 8 9481.0 9 10782.0 10 15240.0 11 17219.0 12 12047.0 13 8159.0 14 7623.0 15 7543.0 16 7805.0 17 8099.0 18 8486.0 19 9071.0 20 9390.0 21 10061.0 22 11144.0 23 12401.0 24 14130.0 25 15906.0 26 18395.0 27 21530.0 28 24474.0 29 28616.0 30 33293.0 31 39713.0 32 48218.0 33 59969.0 34 80696.0 35 105178.0 36 139363.0 37 197706.0 38 195740.0 39 57319.0 40 311.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.65184332643591 28.700274264444797 33.07488398858864 18.572998420530652 2 18.91427342597886 29.13994861869701 33.140098614755786 18.80567934056835 3 19.006082460656856 29.317720561900597 32.5007576290954 19.175439348347144 4 19.536786972363185 28.21935273540425 33.21392302282316 19.029937269409405 5 18.921210599943063 29.241063912501797 33.25606631545759 18.58165917209755 6 19.199361472714514 29.35228202336012 32.47658133838197 18.971775165543402 7 19.709831268060928 28.697745751089776 32.970609663515695 18.621813317333594 8 18.850068379979934 29.597013474720836 32.50165114501071 19.05126700028852 9 18.952160034408102 29.498059569791586 32.50547766878988 19.044302727010436 10-11 19.26576962859134 29.18687604443021 32.56506128152998 18.982293045448476 12-13 19.44551586700279 29.046303821764262 31.927397346239385 19.580782964993563 14-15 19.009710208919863 29.380364854079026 32.525373650584 19.08455128641711 16-17 19.171216602451036 29.228532401792172 32.58667197226803 19.013579023488763 18-19 19.28214056805028 29.203409449647477 31.77132580142156 19.743124180880677 20-21 19.19716760842462 29.480085320744926 31.609392016984554 19.713355053845902 22-23 19.537362517095673 29.339508057831793 31.2602698418894 19.862859583183138 24-25 19.503951148188243 29.864641939990612 30.579640767139978 20.05176614468117 26-27 19.531147154538747 29.75909479156143 30.375210857113117 20.334547196786705 28-29 19.576153740785493 29.934969478202376 30.417915610309688 20.070961170702443 30-31 19.800982346911482 29.92056358873052 30.146081727559242 20.132372336798753 32-33 19.663867604058016 30.06974571206209 29.848968469030346 20.417418214849548 34-35 19.276660793170674 29.662647052633794 30.507512143979092 20.55318001021644 36-37 19.500171994280375 30.155782049766227 30.024193989651266 20.31985196630213 38-39 19.357812207039846 30.0636333372873 30.546642126570266 20.031912329102592 40-41 19.404966203871105 29.442406919549335 30.55575643829169 20.59687043828787 42-43 19.231162176154502 29.883228699482935 30.150160767643975 20.73544835671859 44-45 19.246651555107956 29.613055835729906 30.486498317168255 20.653794291993886 46-47 19.35704098177784 29.33512479445203 30.443256515062167 20.864577708707962 48-49 19.130681692072553 29.363142293624534 30.235108722189953 21.271067292112964 50-51 19.061222177826153 29.581253224455807 30.117335746029994 21.240188851688046 52-53 18.93899525056349 29.193590920764517 30.6869708362629 21.180442992409095 54-55 18.763523696463 29.01466739839097 30.555804311203268 21.666004593942763 56-57 18.988011209283975 29.577095589262324 29.962218605528644 21.47267459592506 58-59 19.25153702423231 28.84998406247357 30.24219300363636 21.656285909657758 60-61 18.75143021361173 28.63760243008121 30.34831010934294 22.26265724696412 62-63 18.935253626668676 28.795521594689472 30.387593457970752 21.8816313206711 64-65 19.003433098962113 28.25917658435929 30.590553486273535 22.146836830405064 66-67 18.928017750924493 28.144690853245276 30.539328224865454 22.387963170964778 68-69 19.22841091505064 28.130820159789582 30.272830336289935 22.367938588869844 70-71 18.796643647889265 27.535485155185352 30.819182654764276 22.84868854216111 72-73 19.077421227445008 27.402694077670127 30.554208950697415 22.965675744187454 74-75 18.837442528262482 27.429321298364428 30.63284295123762 23.10039322213547 76-77 18.841113049205692 27.06253578082129 30.940018419344522 23.156332750628497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25035.0 1 14608.0 2 3800.0 3 3396.5 4 3374.0 5 3419.0 6 3431.5 7 3437.0 8 3515.5 9 3556.0 10 3519.5 11 3533.0 12 3706.0 13 3829.0 14 3999.0 15 4394.0 16 4766.5 17 4914.0 18 5291.0 19 6043.5 20 6822.0 21 7872.0 22 8519.0 23 9174.5 24 10763.0 25 12843.0 26 13990.0 27 15247.0 28 19248.0 29 24758.5 30 27525.0 31 32412.5 32 39674.0 33 46746.0 34 54433.5 35 57423.0 36 61991.0 37 69032.5 38 69714.0 39 67922.0 40 68211.0 41 67594.0 42 65130.5 43 63573.0 44 60172.5 45 55835.5 46 53711.0 47 52273.5 48 52024.0 49 49232.5 50 44265.5 51 39239.0 52 36388.0 53 33810.0 54 29160.5 55 25007.5 56 21095.0 57 19264.0 58 18053.5 59 15089.0 60 12134.5 61 10934.0 62 9448.0 63 6867.5 64 4873.0 65 3973.0 66 3353.5 67 2358.5 68 1596.5 69 1047.5 70 885.0 71 760.5 72 567.0 73 448.5 74 399.0 75 371.5 76 288.0 77 197.0 78 162.0 79 128.5 80 85.5 81 66.0 82 47.5 83 39.0 84 31.5 85 20.5 86 18.5 87 20.0 88 17.5 89 16.5 90 14.5 91 11.0 92 11.0 93 9.0 94 10.5 95 14.5 96 15.0 97 13.5 98 12.0 99 36.0 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008876074693698905 2 0.014002773008162927 3 0.013467147811129374 4 0.014155808778743945 5 0.01224286164648125 6 0.00971777143189449 7 0.014997505516939529 8 0.016451345337459178 9 0.016451345337459178 10-11 0.019167730265272204 12-13 0.01606875591100664 14-15 0.008799556808408398 16-17 0.006810091790855195 18-19 0.01694871159184748 20-21 0.021807597307794725 22-23 0.020430275372565584 24-25 0.01824951564178611 26-27 0.023873580210638432 28-29 0.008646521037827383 30-31 0.008837815751053652 32-33 0.010482950284799569 34-35 0.01274022790086955 36-37 0.011822013277383456 38-39 0.013696701467000897 40-41 0.011592459621511932 42-43 0.014117549836098689 44-45 0.012089825875900233 46-47 0.024141392809155213 48-49 0.02272581193128082 50-51 0.03535126300421461 52-53 0.039674523523128294 54-55 0.029153314295683474 56-57 0.022075409906311502 58-59 0.016795675821266464 60-61 0.019971168060822535 62-63 0.026589965138451462 64-65 0.02590130417083689 66-67 0.027584697647228065 68-69 0.04139617594216472 70-71 0.0538685912445175 72-73 0.08654172826356432 74-75 0.08673302297679059 76-77 0.09117106032364004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1306884.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.87018470337849 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19093799162764 36.179535210946234 2 10.777702269017347 10.103057920537292 3 4.400892675375004 6.188119576637159 4 2.2893253972634553 4.29204416863494 5 1.3662084893963746 3.20172221206659 6 0.877884771781654 2.468797284101364 7 0.5713315733623766 1.8744891459256503 8 0.4075956710222766 1.5283267508089282 9 0.2971227309149545 1.253357754980053 >10 1.532007852701454 12.98956265233288 >50 0.16523137496956697 5.426806799838083 >100 0.11820066849892721 10.20502175912771 >500 0.00506807518059717 1.557200257316525 >1k 1.6348629614829581E-4 0.08045764541788954 >5k 0.0 0.0 >10k+ 3.2697259229659163E-4 2.651500861328792 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20713 1.584914958022288 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13890 1.0628334266851533 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.5910731174304684E-4 0.0 2 0.0 0.0 0.0 0.003443304838072851 0.0 3 0.0 0.0 0.0 0.005203216199754531 0.0 4 0.0 0.0 0.0 0.010635986055380584 0.0 5 0.0 0.0 0.0 0.018899917666755427 0.0 6 0.0 0.0 0.0 0.033514833757242415 0.0 7 0.0 0.0 0.0 0.0588422537884005 7.65178852905078E-5 8 0.0 0.0 0.0 0.08608262095182127 2.2955365587152342E-4 9 0.0 0.0 0.0 0.11967397259435421 2.2955365587152342E-4 10 0.0 0.0 0.0 0.16229443470116706 3.060715411620312E-4 11 0.0 0.0 0.0 0.2125666853370307 3.060715411620312E-4 12 0.0 0.0 0.0 0.27439313665176096 3.060715411620312E-4 13 0.0 0.0 0.0 0.35343611215685555 3.060715411620312E-4 14 0.0 0.0 0.0 0.44456891353785033 3.060715411620312E-4 15 0.0 0.0 0.0 0.5392215376422085 3.060715411620312E-4 16 0.0 0.0 0.0 0.6416789860461984 3.060715411620312E-4 17 0.0 0.0 0.0 0.7285267858509248 3.060715411620312E-4 18 0.0 0.0 0.0 0.8153745856556511 3.060715411620312E-4 19 0.0 0.0 0.0 0.9099506918747188 3.060715411620312E-4 20 0.0 0.0 0.0 1.019677339381307 3.060715411620312E-4 21 0.0 0.0 0.0 1.1375914006139796 3.060715411620312E-4 22 0.0 0.0 0.0 1.2679013592637143 3.82589426452539E-4 23 0.0 0.0 0.0 1.4014250690956505 4.5910731174304684E-4 24 0.0 0.0 0.0 1.5287508302190553 4.5910731174304684E-4 25 0.0 0.0 0.0 1.6579130205894326 4.5910731174304684E-4 26 0.0 0.0 0.0 1.7899063727155586 4.5910731174304684E-4 27 0.0 0.0 0.0 1.9292454418295732 4.5910731174304684E-4 28 0.0 0.0 0.0 2.059555400479308 4.5910731174304684E-4 29 0.0 0.0 0.0 2.1897888412437525 5.356251970335546E-4 30 0.0 0.0 0.0 2.3311938932606107 5.356251970335546E-4 31 0.0 0.0 0.0 2.4993036872438563 5.356251970335546E-4 32 0.0 0.0 0.0 2.66557705198013 5.356251970335546E-4 33 0.0 0.0 0.0 2.840726491410102 5.356251970335546E-4 34 0.0 0.0 0.0 3.0265884347807455 5.356251970335546E-4 35 0.0 0.0 0.0 3.2147459147101043 5.356251970335546E-4 36 0.0 0.0 0.0 3.405198931198178 5.356251970335546E-4 37 0.0 0.0 0.0 3.6091190954973817 5.356251970335546E-4 38 0.0 0.0 0.0 3.806841311088054 6.121430823240624E-4 39 0.0 0.0 0.0 3.997829952773161 6.121430823240624E-4 40 0.0 0.0 0.0 4.2086367267485105 6.121430823240624E-4 41 0.0 0.0 0.0 4.432910648534989 6.121430823240624E-4 42 0.0 0.0 0.0 4.674018505085379 6.121430823240624E-4 43 0.0 0.0 0.0 4.930965563890904 6.121430823240624E-4 44 0.0 0.0 0.0 5.205817807854408 6.121430823240624E-4 45 0.0 0.0 0.0 5.485184607050052 6.121430823240624E-4 46 7.65178852905078E-5 0.0 0.0 5.776029089039272 6.121430823240624E-4 47 7.65178852905078E-5 0.0 0.0 6.08011116518375 6.121430823240624E-4 48 7.65178852905078E-5 0.0 0.0 6.378607435702021 7.65178852905078E-4 49 7.65178852905078E-5 0.0 0.0 6.682153886649465 7.65178852905078E-4 50 7.65178852905078E-5 0.0 0.0 6.983328283152904 7.65178852905078E-4 51 7.65178852905078E-5 0.0 0.0 7.311743046819764 7.65178852905078E-4 52 7.65178852905078E-5 0.0 0.0 7.6328120934987345 7.65178852905078E-4 53 7.65178852905078E-5 0.0 0.0 7.99382347629935 8.416967381955859E-4 54 7.65178852905078E-5 0.0 0.0 8.384370762822103 8.416967381955859E-4 55 7.65178852905078E-5 0.0 0.0 8.781115998053385 8.416967381955859E-4 56 7.65178852905078E-5 0.0 0.0 9.205637225645122 8.416967381955859E-4 57 7.65178852905078E-5 0.0 0.0 9.619981574493222 8.416967381955859E-4 58 7.65178852905078E-5 0.0 0.0 10.039070032229333 8.416967381955859E-4 59 7.65178852905078E-5 0.0 0.0 10.462366973656422 9.182146234860937E-4 60 7.65178852905078E-5 0.0 0.0 10.886352576051125 9.182146234860937E-4 61 7.65178852905078E-5 0.0 0.0 11.342246136611971 9.182146234860937E-4 62 7.65178852905078E-5 0.0 0.0 11.787350675346856 9.182146234860937E-4 63 7.65178852905078E-5 0.0 0.0 12.262220671459747 9.182146234860937E-4 64 7.65178852905078E-5 0.0 0.0 12.742370401657684 9.182146234860937E-4 65 7.65178852905078E-5 0.0 0.0 13.251367374610142 9.182146234860937E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 50 0.006735368 28.393862 4 CGTAGGC 310 0.0 22.898275 4 TACGTAG 315 0.0 22.5374 2 CGCCGGA 80 0.0024469998 22.202244 71 GGGTCGC 85 0.0034792651 20.895432 69 ACGTAGG 385 0.0 20.28288 3 ATACGTA 360 0.0 19.720224 1 CGTGTGA 130 9.497029E-5 19.112717 14 CAACCGG 95 0.0066453614 18.683746 60 ACGAAGC 250 7.185008E-10 18.457422 42 CTATTCG 155 1.759532E-5 18.31932 39 GGACGAA 335 0.0 18.0131 37 TTACGAG 160 2.3052819E-5 17.752956 51 CGAGCCG 180 3.1945965E-6 17.75092 54 GCCCTTA 100 0.008936198 17.748201 26 CAACGAA 300 2.3646862E-11 17.7482 40 TTACGGC 430 0.0 17.336779 58 CGAACAT 350 0.0 17.24111 40 AGACGTC 375 0.0 17.05067 68 TAGTCGG 420 0.0 16.90305 16 >>END_MODULE