FastQCFastQC Report
Thu 26 May 2016
SRR936077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306884
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127450.975220448027522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96310.7369437532328806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.5665384226909198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40830.31242252564114337No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.21792293730736623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.1902234628322024No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20820.15931023717483725No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.15234710961340103No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.14178764144331096No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14560.11141004098297937TruSeq Adapter, Index 1 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.10077405492759878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10050.053.010273100-101
CGGTATC9600.048.36447100-101
CGAGTAC6300.048.187313100-101
TTACGCA2900.047.209885100-101
ACGTATC3050.046.83974100-101
CCGTACA2950.046.409714100-101
ACCGTAC1700.045.5203898-99
CCTGTCG5900.042.872896-97
TCGGTAT2650.042.678665100-101
CGAGCGT1550.042.23824796-97
TTTACGC2400.042.16470798-99
CGTACAT10000.041.66785100-101
GGTATCA84150.041.440211
CCGGTAT4100.041.377487100-101
CCCGGTA2400.040.9245798-99
CACGCAG6150.040.651566100-101
CTCGGTA1800.039.6844398-99
ACGGTAT2250.039.68367100-101
CCGAGCG3700.039.41025596-97
TACGGTA1150.038.82172498-99
Produced by FastQC (version 0.11.5)