FastQCFastQC Report
Thu 26 May 2016
SRR936076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313160
Sequences flagged as poor quality0
Sequence length77
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209891.5983581589448355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139731.0640744463736331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCAA958.41938E-626.1578824
ATACCCG750.001687954923.66665321
TGAGCGA850.003491933720.88234120
CGATTGC3950.020.6795468
GAACGAT4100.019.9244965
TACGTAG1801.4710349E-719.7169532
GCATATC2950.019.2505594
TCGTGGA4800.019.23281946
TTTCGTG4800.019.23135444
GTGAACG4100.019.05457763
TAGGGCG2052.7619535E-819.0451437
TCGTACC3750.018.93620949
CGTACCA3800.018.68704850
ACCCGTA1902.5892768E-718.68562357
CTCGATA950.00665322118.679935
AGTCGTG1159.1792905E-418.51749611
TGTTTCG4800.018.49098242
AATACGT1351.2690268E-418.403898
CCCGTAA1953.4029654E-718.2058158
TAACGCG2154.7477442E-818.1655552
Produced by FastQC (version 0.11.5)