##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936076_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1313160 Sequences flagged as poor quality 0 Sequence length 77 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.719655639830634 40.0 36.0 41.0 30.0 41.0 2 36.57484160346036 39.0 36.0 41.0 30.0 41.0 3 36.421205336744954 39.0 36.0 41.0 30.0 41.0 4 36.32461010082549 39.0 35.0 41.0 30.0 41.0 5 36.23046163453044 39.0 35.0 41.0 30.0 41.0 6 36.10435666636206 39.0 35.0 40.0 30.0 41.0 7 35.902184806116544 39.0 35.0 40.0 29.0 41.0 8 35.689511559901305 38.0 35.0 40.0 29.0 41.0 9 35.77664641018612 39.0 35.0 40.0 29.0 41.0 10-11 35.89799262846873 39.0 35.0 41.0 29.5 41.0 12-13 35.73354770172713 39.0 35.0 41.0 28.5 41.0 14-15 35.530266684943186 39.0 35.0 41.0 27.0 41.0 16-17 35.31513448475434 39.0 35.0 41.0 26.5 41.0 18-19 35.04745804014743 39.0 35.0 41.0 25.0 41.0 20-21 34.685693289469675 39.0 34.5 41.0 23.5 41.0 22-23 34.221638261902584 38.0 34.0 41.0 20.5 41.0 24-25 33.86101960157178 38.0 33.0 40.0 14.5 41.0 26-27 32.873650964086636 37.5 32.0 39.5 9.5 40.5 28-29 32.87622148100765 37.5 33.0 39.5 8.0 40.5 30-31 33.27581444759207 38.0 33.0 40.0 7.0 41.0 32-33 33.161650141643065 38.0 33.0 40.0 2.0 41.0 34-35 32.92108958542752 38.0 33.0 40.0 2.0 41.0 36-37 32.62900293947425 37.5 32.5 40.0 2.0 41.0 38-39 32.31680107526881 37.0 32.0 40.0 2.0 41.0 40-41 31.95841900453867 36.5 31.5 40.0 2.0 41.0 42-43 31.58194736361144 36.0 31.0 39.0 2.0 41.0 44-45 31.19772876115629 35.5 31.0 39.0 2.0 41.0 46-47 30.754807487282584 35.0 30.5 39.0 2.0 40.0 48-49 30.309611166956046 35.0 30.0 38.0 2.0 40.0 50-51 29.83309764232843 35.0 29.5 37.0 2.0 39.5 52-53 29.321277300557433 35.0 29.0 37.0 2.0 39.0 54-55 28.810634271528222 34.5 29.0 36.0 2.0 39.0 56-57 28.312280300953425 34.0 27.5 36.0 2.0 37.5 58-59 27.831302354625485 34.0 26.5 35.0 2.0 37.0 60-61 27.40392183739986 34.0 26.0 35.0 2.0 36.5 62-63 26.988048676475067 34.0 25.5 35.0 2.0 36.0 64-65 26.548419461451765 34.0 25.0 35.0 2.0 36.0 66-67 26.13956296262451 33.0 23.5 35.0 2.0 35.5 68-69 25.713089037131805 33.0 21.0 35.0 2.0 35.0 70-71 25.224459700265008 33.0 18.5 35.0 2.0 35.0 72-73 24.706701392061895 33.0 4.5 35.0 2.0 35.0 74-75 24.128319854397027 32.0 2.0 35.0 2.0 35.0 76-77 22.45277003563922 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7910007013751894 1101 2 0.7997653157434073 1101 3 0.8904936071987279 1101 4 0.6803018593243024 1101 5 0.5904942341020814 1101 6 0.6330781551278619 1101 7 0.7103383101503837 1101 8 0.834282270803719 1101 9 0.7639444334172296 1101 10-11 0.7404064405085791 1101 12-13 1.0052141739608942 1101 14-15 1.0850049242513222 1101 16-17 1.1341276665447495 1101 18-19 1.0922827629070113 1101 20-21 1.0798274713560687 1101 22-23 1.115919052041292 1101 24-25 1.1925407398566392 1101 26-27 1.188534677316163 1101 28-29 1.360656842082328 1101 30-31 1.462372832084462 1101 32-33 1.525212657726307 1101 34-35 1.5554581231500677 1101 36-37 1.5291955635085728 1101 38-39 1.4867707709168272 1101 40-41 1.406106695375584 1101 42-43 1.3427578168624628 1101 44-45 1.3461269584444011 1101 46-47 1.3113676198877506 1101 48-49 1.4547661970584258 1101 50-51 1.2603452183510093 1101 52-53 1.2914404630631893 1101 54-55 1.2710933577133723 1101 56-57 1.2429667785938214 1101 58-59 1.3947410446422026 1101 60-61 1.377945408284866 1101 62-63 1.2561872946972272 1101 64-65 1.2209377045462446 1101 66-67 1.044896413103011 1101 68-69 1.2372744738922243 1101 70-71 0.8837058957136321 1101 72-73 1.0716140844375737 1101 74-75 0.961302759239242 1101 76-77 0.9316593093231553 1102 1 0.9202095569386515 1102 2 0.8973147109598756 1102 3 1.1328065621546628 1102 4 0.8865522013964409 1102 5 0.9371446637446823 1102 6 1.045769031379841 1102 7 1.0458371881537758 1102 8 1.1522916846289064 1102 9 0.9569292180486144 1102 10-11 1.1326482444601993 1102 12-13 1.2842205228197443 1102 14-15 1.3182666504841478 1102 16-17 1.2690313390312014 1102 18-19 1.3123411888278653 1102 20-21 1.462361952227397 1102 22-23 1.3180713699220874 1102 24-25 1.5755055474752737 1102 26-27 1.3037377313361915 1102 28-29 1.4637033365270682 1102 30-31 1.5463925901710454 1102 32-33 1.6041841152272696 1102 34-35 1.51281335822204 1102 36-37 1.4194075114041524 1102 38-39 1.4020265040458284 1102 40-41 1.3951583345852612 1102 42-43 1.603819061457724 1102 44-45 1.4844691400436574 1102 46-47 1.3520488907541512 1102 48-49 1.233354533766871 1102 50-51 1.164468555880429 1102 52-53 1.1140623775725196 1102 54-55 1.2989968660052007 1102 56-57 1.3383022829449764 1102 58-59 1.2746812504327991 1102 60-61 1.1255385887347629 1102 62-63 1.2040705523185942 1102 64-65 1.2247381259791155 1102 66-67 1.2766509695038692 1102 68-69 1.2691412299537426 1102 70-71 1.42908518529822 1102 72-73 1.2667060334277735 1102 74-75 1.1643759353106091 1102 76-77 0.9936824607653314 1103 1 0.7792163135142474 1103 2 0.8074609693720802 1103 3 0.7634283279803356 1103 4 0.8229090129362433 1103 5 0.741165094342314 1103 6 0.7220399110752638 1103 7 0.8390564819307542 1103 8 0.7335193973135716 1103 9 0.9978956496650966 1103 10-11 0.9978143436102656 1103 12-13 0.8535980866988737 1103 14-15 0.8773109435908353 1103 16-17 0.8223499705565231 1103 18-19 0.7290177982066339 1103 20-21 0.9243451546297337 1103 22-23 0.64405670386315 1103 24-25 0.7609467357745956 1103 26-27 0.8030560411198238 1103 28-29 0.8201693391546243 1103 30-31 1.1172729558841041 1103 32-33 1.0762498979980037 1103 34-35 1.1070256943348582 1103 36-37 1.0994786297246577 1103 38-39 0.9779759771938004 1103 40-41 1.0209409042289366 1103 42-43 0.9558956359811397 1103 44-45 1.0206476530607915 1103 46-47 1.0325925671892549 1103 48-49 1.1687873433762235 1103 50-51 1.1653959092868682 1103 52-53 1.1952130063094195 1103 54-55 1.1244593696918237 1103 56-57 1.2502006930047678 1103 58-59 1.1721927795102047 1103 60-61 0.9452271695857704 1103 62-63 0.7300438209460971 1103 64-65 0.8651592724520221 1103 66-67 0.9418649947605893 1103 68-69 0.6895513835853997 1103 70-71 0.6059915048413487 1103 72-73 0.7320283113589774 1103 74-75 0.8427562339941481 1103 76-77 0.7814167289370353 1104 1 0.5856602810606546 1104 2 0.5122481671487691 1104 3 0.4059627993756507 1104 4 0.45754111784226836 1104 5 0.7272674484943025 1104 6 0.33210325338018976 1104 7 0.3617184820671113 1104 8 0.48755061068720806 1104 9 0.3839733292134184 1104 10-11 0.48092782406051526 1104 12-13 0.7863016765290425 1104 14-15 0.9048260486689301 1104 16-17 0.7176663411820599 1104 18-19 0.3399846855378499 1104 20-21 0.2665711934792512 1104 22-23 0.6007225805096184 1104 24-25 0.5993189911307724 1104 26-27 0.4972292633308584 1104 28-29 0.6033328181438762 1104 30-31 0.7585448018366563 1104 32-33 0.8384811964340884 1104 34-35 0.9446122583638328 1104 36-37 0.9999601091619468 1104 38-39 0.8435188671742893 1104 40-41 0.880584615938659 1104 42-43 0.8752785111568677 1104 44-45 0.7028462251804335 1104 46-47 0.6075624544328093 1104 48-49 0.552286526841641 1104 50-51 0.5764251324589189 1104 52-53 0.60643280832819 1104 54-55 0.656949329400792 1104 56-57 0.5713085177761208 1104 58-59 0.6131422322859379 1104 60-61 0.694735787840429 1104 62-63 0.39488413405383227 1104 64-65 0.40944466670998025 1104 66-67 0.12468724995443381 1104 68-69 0.04288131874963241 1104 70-71 -0.071696160129477 1104 72-73 -0.0750698578503659 1104 74-75 -0.1307416183413359 1104 76-77 0.09517704113570602 1105 1 -0.09824893266468138 1105 2 0.16865181230699733 1105 3 0.2117849377181571 1105 4 0.31677118103444 1105 5 0.31093417397302403 1105 6 0.0687420363401543 1105 7 0.1667125806917369 1105 8 0.20655660633171635 1105 9 0.3782592182064164 1105 10-11 0.2550701182273514 1105 12-13 0.31242473724409336 1105 14-15 0.45087938843974484 1105 16-17 0.3445255292063152 1105 18-19 0.3030856433250264 1105 20-21 0.3766976096660244 1105 22-23 0.36718990192215273 1105 24-25 0.3887468984896927 1105 26-27 0.36244286891516 1105 28-29 0.40135259300624426 1105 30-31 0.41102353873213104 1105 32-33 0.5647366798086395 1105 34-35 0.4798937568354589 1105 36-37 0.44284702505158435 1105 38-39 0.4427607012695276 1105 40-41 0.5359936580049904 1105 42-43 0.5849193775921471 1105 44-45 0.3148519730499828 1105 46-47 0.30818819767807426 1105 48-49 0.20170788631725145 1105 50-51 0.43791924732586196 1105 52-53 0.39742284446256804 1105 54-55 0.18246569178409544 1105 56-57 0.37120380259949215 1105 58-59 0.39119828802702017 1105 60-61 0.44028236730389736 1105 62-63 0.38342884151058243 1105 64-65 0.5005427301798626 1105 66-67 0.460894942698193 1105 68-69 0.48002386833487876 1105 70-71 0.5105562098157357 1105 72-73 0.14076315329058886 1105 74-75 0.08508852650829013 1105 76-77 -0.020395647057021904 1106 1 -0.021981392288722645 1106 2 -0.06638349756449458 1106 3 -0.39121067336919424 1106 4 -0.22182782916902255 1106 5 -0.10207039823528419 1106 6 -0.08012776773715302 1106 7 -0.6721699013351028 1106 8 -0.09276265396847805 1106 9 0.012031951959293963 1106 10-11 -0.17524571037084513 1106 12-13 0.11799164270427553 1106 14-15 -0.09554246396651678 1106 16-17 -0.08126423322215004 1106 18-19 -0.022926365086213707 1106 20-21 -0.0018483338992894005 1106 22-23 0.3440440520412906 1106 24-25 0.28764629717810664 1106 26-27 -0.0179080277060919 1106 28-29 0.15943637694140733 1106 30-31 0.16796458881258047 1106 32-33 0.23911258355805387 1106 34-35 0.23997947301231903 1106 36-37 0.3800412140701397 1106 38-39 0.40067334561485524 1106 40-41 0.4167531511924949 1106 42-43 0.4694583890175785 1106 44-45 0.1804781186478337 1106 46-47 0.20903065760760953 1106 48-49 0.04358069695247124 1106 50-51 0.0696890941721584 1106 52-53 -0.03814141339368504 1106 54-55 -0.03454872747416715 1106 56-57 0.02210099664849352 1106 58-59 -0.0462746631348665 1106 60-61 -0.2576785321686188 1106 62-63 -0.03012402655727442 1106 64-65 -0.17187261013906863 1106 66-67 -0.20408642224345996 1106 68-69 -0.19173657188018467 1106 70-71 -0.2489427056849678 1106 72-73 -0.4301891312860988 1106 74-75 -0.4569148186374292 1106 76-77 -0.5788017258537863 1107 1 0.16867039792812477 1107 2 0.11820594539295115 1107 3 0.3246942298466067 1107 4 0.05744017967538895 1107 5 0.20682686310146892 1107 6 -0.16776329471647955 1107 7 -0.12160427621387981 1107 8 0.003192386956300197 1107 9 -0.050000128416208156 1107 10-11 0.09942795811901561 1107 12-13 0.11435071889031434 1107 14-15 -0.06869345411354288 1107 16-17 -0.08029263327646419 1107 18-19 -0.10553296832915748 1107 20-21 -0.273949050567893 1107 22-23 -0.1482261824519 1107 24-25 0.06523112882402415 1107 26-27 -0.2507665317209842 1107 28-29 -0.26293563613125315 1107 30-31 0.087358766243824 1107 32-33 0.22962933168637534 1107 34-35 -0.037709004042646654 1107 36-37 0.21069491960565045 1107 38-39 0.13940327113562745 1107 40-41 0.07224490319994459 1107 42-43 0.08650133094705481 1107 44-45 0.29652642831849363 1107 46-47 0.21945641597682553 1107 48-49 0.0932353334783258 1107 50-51 0.03444844286683235 1107 52-53 0.19860591485798906 1107 54-55 -0.025893426647915163 1107 56-57 0.08354454081234763 1107 58-59 0.2293136306313741 1107 60-61 0.14936487040712265 1107 62-63 0.0047450771705648265 1107 64-65 -0.08472864121087298 1107 66-67 -0.07403870898848197 1107 68-69 -0.08361986351291151 1107 70-71 -0.14868304666017806 1107 72-73 -0.28472492107623637 1107 74-75 -0.20548560299679153 1107 76-77 -0.2050594113730071 1108 1 0.05933883243037741 1108 2 0.3072714289696421 1108 3 0.24957368272415437 1108 4 0.1474173451323324 1108 5 0.055956827348126126 1108 6 0.08127204499692908 1108 7 0.10572011595098019 1108 8 0.1479820881807541 1108 9 0.12547908535024277 1108 10-11 0.07261153537966436 1108 12-13 0.052607927636564966 1108 14-15 0.22956647437977296 1108 16-17 0.15006229371532953 1108 18-19 0.25733058536035003 1108 20-21 0.3052182535394934 1108 22-23 0.12734227301506706 1108 24-25 0.23142240636853728 1108 26-27 0.11974772711960924 1108 28-29 0.34154798022576216 1108 30-31 0.31780973430379333 1108 32-33 0.3665272335148373 1108 34-35 0.581248199604083 1108 36-37 0.5550367124855811 1108 38-39 0.4217461632466595 1108 40-41 0.49494657527777974 1108 42-43 0.49715933975224047 1108 44-45 0.5211571376625272 1108 46-47 0.8301421888666134 1108 48-49 0.831980113547111 1108 50-51 0.6855796876610647 1108 52-53 0.6118864964305715 1108 54-55 0.5950897052541535 1108 56-57 0.5726817010564424 1108 58-59 0.6754634886945752 1108 60-61 0.7442112330287287 1108 62-63 0.8116659835262716 1108 64-65 0.8026367171441322 1108 66-67 0.8769420997962953 1108 68-69 0.7247725393269597 1108 70-71 0.7860382075965084 1108 72-73 0.5697847595234649 1108 74-75 0.45716433722543215 1108 76-77 0.5707994337543951 1109 1 -0.39260040388730744 1109 2 -0.4067562331244474 1109 3 -0.16395932537275826 1109 4 0.08240235819278752 1109 5 -0.0306920476398318 1109 6 -0.3273699512974204 1109 7 -0.17909292976909796 1109 8 -0.03614195494989758 1109 9 -0.1074887819714263 1109 10-11 -0.24323467425617196 1109 12-13 -0.21015760183585286 1109 14-15 -0.13450283384122486 1109 16-17 0.06229435613352763 1109 18-19 -0.0726282784653165 1109 20-21 0.04980284050623851 1109 22-23 0.011002259303019457 1109 24-25 -0.08151622367219602 1109 26-27 -0.05863340010222373 1109 28-29 0.10052881167509753 1109 30-31 0.024196890298284757 1109 32-33 0.10254915014326116 1109 34-35 0.24784318960335128 1109 36-37 0.23792638707917746 1109 38-39 0.3651975222702717 1109 40-41 0.25076230108194864 1109 42-43 0.32250738238427346 1109 44-45 0.38843263104164194 1109 46-47 0.29358652187697487 1109 48-49 0.3969418387733299 1109 50-51 0.27953165012855763 1109 52-53 0.2973199844495191 1109 54-55 0.3492956807650991 1109 56-57 0.0986731338531932 1109 58-59 0.06970697405044035 1109 60-61 -0.009107141695750443 1109 62-63 -0.07846218455409826 1109 64-65 -0.022323194917380107 1109 66-67 -0.0755515682925676 1109 68-69 -0.2731054913495221 1109 70-71 -0.282571095071674 1109 72-73 -0.3422135746314581 1109 74-75 -0.1695551945247793 1109 76-77 -0.16043006190250964 1110 1 -0.33232621987492905 1110 2 -0.42682226856361183 1110 3 -0.5010139448103956 1110 4 -0.5349909591424975 1110 5 -0.4975721665818753 1110 6 -0.36856167578313404 1110 7 -0.3483690613704695 1110 8 -0.6708462083451394 1110 9 -0.54168157589217 1110 10-11 -0.3970626864981597 1110 12-13 -0.3238877781622165 1110 14-15 -0.18043691577907595 1110 16-17 -0.2471158026297573 1110 18-19 -0.3219289070844411 1110 20-21 -0.4092173657295177 1110 22-23 -0.17625048818859312 1110 24-25 -0.15879214225601856 1110 26-27 -0.23247201974853482 1110 28-29 -0.06225626231589132 1110 30-31 0.061062511005332 1110 32-33 0.018981062779978686 1110 34-35 0.12360016266749341 1110 36-37 0.30382271274387307 1110 38-39 0.2937160069764815 1110 40-41 0.16223557824820034 1110 42-43 0.03568849511837513 1110 44-45 0.07444639893076754 1110 46-47 0.008558177501509334 1110 48-49 -0.043551818520562335 1110 50-51 -0.014417890796895705 1110 52-53 -0.18749762471110643 1110 54-55 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-0.7347387723286474 2314 34-35 -0.8081771587503326 2314 36-37 -1.307381174841126 2314 38-39 -0.9507286882151931 2314 40-41 -0.6839629684208717 2314 42-43 -0.46094334275849747 2314 44-45 -0.5865690521133438 2314 46-47 -0.37824825566934095 2314 48-49 -0.399631059659157 2314 50-51 -0.3160799687893139 2314 52-53 -0.07481370162029677 2314 54-55 -0.2851780065720355 2314 56-57 -0.18849983903414724 2314 58-59 -0.27566157748578846 2314 60-61 -0.11270731119936173 2314 62-63 -0.08827151279704282 2314 64-65 -0.0993859508559467 2314 66-67 -0.11810172631111726 2314 68-69 -0.057366606112967844 2314 70-71 0.09176158348136454 2314 72-73 0.11205856974759598 2314 74-75 -0.22238249886296657 2314 76-77 -0.21651526122766285 2315 1 -1.1668709103877646 2315 2 -1.014473125846493 2315 3 -1.0008416458832414 2315 4 -0.877916078028754 2315 5 -1.0478576084711904 2315 6 -0.8903723269199233 2315 7 -1.2940752763842127 2315 8 -1.5335435863383537 2315 9 -0.9500979955970408 2315 10-11 -1.1858017072276397 2315 12-13 -1.0810762329854384 2315 14-15 -0.9731323506232812 2315 16-17 -1.1511548723687 2315 18-19 -1.011380910346638 2315 20-21 -1.0674346844519107 2315 22-23 -1.1641275835349916 2315 24-25 -1.2552783449408196 2315 26-27 -1.2688601173386402 2315 28-29 -1.2072072815597679 2315 30-31 -1.4277995598746216 2315 32-33 -1.1868721232186275 2315 34-35 -1.3038593566567869 2315 36-37 -1.2237776609533526 2315 38-39 -1.3057066958959354 2315 40-41 -1.3318495431651058 2315 42-43 -1.4120180625787917 2315 44-45 -1.1705787088517816 2315 46-47 -1.2174817422454822 2315 48-49 -1.0944013000922332 2315 50-51 -0.9667262656734152 2315 52-53 -1.0533751144306045 2315 54-55 -1.2210333787323044 2315 56-57 -1.1867797815506584 2315 58-59 -1.1389433861229605 2315 60-61 -1.1868232583340799 2315 62-63 -1.0526087696345385 2315 64-65 -0.8203529501687967 2315 66-67 -0.7784599168368089 2315 68-69 -1.0250696382974454 2315 70-71 -0.9412744204392354 2315 72-73 -0.9481336319608609 2315 74-75 -1.0573206378348878 2315 76-77 -1.1245946480936624 2316 1 -2.383153958660401 2316 2 -2.1864410764163225 2316 3 -2.399422041646929 2316 4 -3.139742510358033 2316 5 -2.5592266269623067 2316 6 -2.577638829684055 2316 7 -2.3904235319228064 2316 8 -2.153433178134584 2316 9 -2.5805763983924237 2316 10-11 -2.2282154176547095 2316 12-13 -2.9080571149539978 2316 14-15 -4.261567395893003 2316 16-17 -3.492602019698211 2316 18-19 -3.00036637529724 2316 20-21 -2.814288060470645 2316 22-23 -2.9501576441239017 2316 24-25 -2.9869346252180797 2316 26-27 -2.9833429891310956 2316 28-29 -3.4177738851416457 2316 30-31 -3.3412395182897576 2316 32-33 -3.4995063749170967 2316 34-35 -3.4452159547162893 2316 36-37 -3.456387337098459 2316 38-39 -3.515376404782245 2316 40-41 -3.1119830922837863 2316 42-43 -3.243101175311896 2316 44-45 -3.2538486626092578 2316 46-47 -3.192642107311837 2316 48-49 -3.074757316435324 2316 50-51 -3.202200796910212 2316 52-53 -3.257299145410933 2316 54-55 -3.2677288953888493 2316 56-57 -3.137981684313612 2316 58-59 -3.345411689456629 2316 60-61 -3.2911231940701384 2316 62-63 -3.1277634354522164 2316 64-65 -3.3005492892858825 2316 66-67 -3.1100031456380606 2316 68-69 -3.0065588716305847 2316 70-71 -3.0054735089524947 2316 72-73 -3.2587608637571677 2316 74-75 -3.3659870332959265 2316 76-77 -3.6273005629311683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31824.0 3 3411.0 4 5456.0 5 6922.0 6 7372.0 7 7691.0 8 9819.0 9 10978.0 10 15170.0 11 17341.0 12 11800.0 13 8367.0 14 7705.0 15 7627.0 16 8019.0 17 8215.0 18 8436.0 19 9418.0 20 9857.0 21 10441.0 22 11312.0 23 12399.0 24 14297.0 25 16078.0 26 18541.0 27 21533.0 28 24849.0 29 28690.0 30 33672.0 31 39894.0 32 47901.0 33 59723.0 34 80753.0 35 104067.0 36 139929.0 37 199132.0 38 197806.0 39 56428.0 40 287.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.610287543731655 28.831136786985518 33.03409736490593 18.5244783043769 2 18.90597808909824 29.186966174045825 33.1672562631136 18.739799473742334 3 19.0988319178801 29.22611989121155 32.457785830540125 19.217262360368224 4 19.49931374236624 28.13778164363654 33.31777003590475 19.045134578092462 5 18.894808394612276 29.163927996130955 33.33404418176902 18.60721942748775 6 19.222186341383164 29.224714925041205 32.541272273623214 19.011826459952413 7 19.656207728585528 28.691475036254406 32.96477229798072 18.687544937179347 8 18.749704856800328 29.641288667567455 32.48684994234235 19.122156533289868 9 18.976139681156617 29.470353780429008 32.51788171978311 19.035624818631263 10-11 19.28594578269892 29.180197633681892 32.57611966332123 18.957736920297954 12-13 19.432988689555156 29.02494970110096 31.9559388118606 19.586122797483284 14-15 19.065690317618074 29.307873622963243 32.51950603744702 19.10693002197166 16-17 19.214805540578002 29.220509703931835 32.580482774797495 18.984201980692664 18-19 19.339414814518634 29.146859964089938 31.794113110980366 19.71961211041106 20-21 19.169951744391504 29.54540261750119 31.568924180203016 19.71572145790429 22-23 19.554441023414697 29.3183518887702 31.217804079720786 19.90940300809432 24-25 19.511879294802192 29.7892031200787 30.60281379881883 20.09610378630028 26-27 19.636993294254246 29.71114911630312 30.38248531868572 20.269372270756907 28-29 19.599573207092806 29.919789741281548 30.404653596324284 20.075983455301362 30-31 19.78745733229555 29.896942547678922 30.179033002450772 20.136567117574753 32-33 19.644957660793594 30.074488214822775 29.858955626561993 20.421598497821634 34-35 19.248067904654736 29.642407660217778 30.559455605123315 20.55006883000417 36-37 19.479471543322283 30.200124749529905 30.00035795042158 20.32004575672623 38-39 19.384545313294968 30.031806286311415 30.570555726502498 20.013092673891123 40-41 19.434758154159706 29.41590431955022 30.588756777917364 20.560580748372708 42-43 19.259802566287902 29.833039719044258 30.149122546454656 20.758035168213187 44-45 19.31600586789915 29.629920192306226 30.474535115232925 20.5795388245617 46-47 19.423368624937495 29.35681079975642 30.39171890715227 20.828101668153813 48-49 19.148274198296306 29.349920161892566 30.213192309493863 21.28861333031727 50-51 19.02847222857004 29.601009454687276 30.126151366404937 21.244366950337753 52-53 18.894011849464945 29.282918830593786 30.68139184554179 21.141677474399483 54-55 18.82973516983446 29.018126974514836 30.549103172744218 21.603034682906486 56-57 18.99273057822159 29.63992580155381 29.920016938754294 21.447326681470305 58-59 19.2452406250119 28.881665885973028 30.171219443460647 21.701874045554426 60-61 18.797976074229908 28.600633409856396 30.39963622348796 22.20175429242574 62-63 18.958808216647718 28.77174941776211 30.385790919945382 21.883651445644784 64-65 19.092522488898368 28.227014098882602 30.518825169285613 22.16163824293342 66-67 18.994785948147396 28.175721659280317 30.503745731283054 22.325746661289237 68-69 19.282669710643216 28.1074593803192 30.270769940960108 22.339100968077478 70-71 18.770884805214845 27.600459460952486 30.799656356733713 22.828999377098956 72-73 19.12123259251131 27.463796195642853 30.524435543759097 22.890535668086738 74-75 18.867571500811103 27.43692270523485 30.57463967127518 23.120866122678866 76-77 18.85892596054516 27.039873612829545 31.021023755895342 23.080176670729948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25310.0 1 14727.5 2 3786.5 3 3450.5 4 3473.0 5 3462.0 6 3405.5 7 3354.5 8 3399.5 9 3450.0 10 3442.5 11 3499.0 12 3665.5 13 3768.0 14 3965.0 15 4357.0 16 4755.0 17 4958.0 18 5226.0 19 5951.5 20 6865.0 21 7819.5 22 8318.0 23 9004.5 24 10830.0 25 13025.5 26 14082.0 27 15464.0 28 19532.5 29 25069.0 30 27919.0 31 32727.0 32 39774.0 33 46949.5 34 54844.0 35 57802.0 36 62002.0 37 69208.0 38 70348.0 39 68482.0 40 68582.5 41 67568.0 42 65367.0 43 64281.0 44 61132.5 45 56227.5 46 53617.5 47 52680.0 48 52596.0 49 49777.5 50 44824.5 51 39457.0 52 36224.0 53 33680.5 54 29007.0 55 24939.0 56 21163.5 57 19326.0 58 18038.5 59 15069.0 60 12290.5 61 11194.0 62 9660.5 63 6962.0 64 4953.0 65 4109.0 66 3505.5 67 2379.5 68 1541.0 69 1080.5 70 936.0 71 795.0 72 575.5 73 458.0 74 419.0 75 370.5 76 254.0 77 159.5 78 133.0 79 111.5 80 72.0 81 51.5 82 40.5 83 32.0 84 30.5 85 25.0 86 14.5 87 8.0 88 8.5 89 13.0 90 17.5 91 18.0 92 12.5 93 8.0 94 7.5 95 7.5 96 9.0 97 8.5 98 10.0 99 23.0 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0028937829358189407 2 0.008757500989978373 3 0.011651283925797314 4 0.018428828170215357 5 0.013326631941271438 6 0.013098175393706784 7 0.01644887142465503 8 0.018124219440129152 9 0.01728654543239209 10-11 0.02105607846720887 12-13 0.01968533918182095 14-15 0.007995979164762862 16-17 0.00639678333181029 18-19 0.011993968747144293 20-21 0.011575131743275763 22-23 0.015420816960614092 24-25 0.0234929483078985 26-27 0.01382162112766152 28-29 0.008452892259892169 30-31 0.008072131347284413 32-33 0.010166316366627067 34-35 0.010851686009321026 36-37 0.009823631545280088 38-39 0.016144262694568826 40-41 0.01252703402479515 42-43 0.016220414877090375 44-45 0.01934265436047397 46-47 0.018390752078954582 48-49 0.01450699077035548 50-51 0.028328611898016994 52-53 0.03369734076578635 54-55 0.025701361601023483 56-57 0.014811599500441681 58-59 0.01713424106734899 60-61 0.020256480550732585 62-63 0.025701361601023483 64-65 0.02208413293124981 66-67 0.020218404459471807 68-69 0.030727405647445853 70-71 0.05730451734746718 72-73 0.06865119254317828 74-75 0.08068323738158335 76-77 0.07626641079533339 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1313160.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.776599757513644 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36041377940298 36.18657112434776 2 10.601249240839234 9.917807853367643 3 4.411282918485377 6.190344464854413 4 2.235048072759729 4.181917965531364 5 1.3996388478149164 3.2735173094652943 6 0.8676199160640132 2.4350585733224355 7 0.5790720154364257 1.8960913927792513 8 0.4123594105401322 1.5431016882464013 9 0.3066221969512473 1.2908469405202216 >10 1.5405402591030013 13.10883381794816 >50 0.16001936609405856 5.239052368346551 >100 0.12042286925048476 10.368176882171035 >500 0.005221583779121163 1.6227222150753846 >1k 1.6317449309753633E-4 0.0773960642561825 >5k 0.0 0.0 >10k+ 3.2634898619507267E-4 2.668561339767902 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20989 1.5983581589448355 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13973 1.0640744463736331 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.092174601724085E-4 0.0 2 0.0 0.0 0.0 0.0036553047610344514 0.0 3 0.0 0.0 0.0 0.005482957141551677 0.0 4 0.0 0.0 0.0 0.010889762100581802 0.0 5 0.0 0.0 0.0 0.01926650217795242 0.0 6 0.0 0.0 0.0 0.03289774284931006 0.0 7 0.0 0.0 0.0 0.060160224192025345 0.0 8 0.0 0.0 0.0 0.08985957537543025 0.0 9 0.0 0.0 0.0 0.12428036187517134 0.0 10 0.0 0.0 0.0 0.17042858448323128 7.615218252155107E-5 11 0.0 0.0 0.0 0.22091748149501964 7.615218252155107E-5 12 0.0 0.0 0.0 0.28260074933747603 7.615218252155107E-5 13 0.0 0.0 0.0 0.36316975844527705 7.615218252155107E-5 14 0.0 0.0 0.0 0.4505924639800177 1.5230436504310213E-4 15 0.0 0.0 0.0 0.549437996892991 1.5230436504310213E-4 16 0.0 0.0 0.0 0.6455420512351884 2.2845654756465322E-4 17 0.0 0.0 0.0 0.7365439093484419 2.2845654756465322E-4 18 0.0 0.0 0.0 0.8282311371043894 2.2845654756465322E-4 19 0.0 0.0 0.0 0.9212129519632033 2.2845654756465322E-4 20 0.0 0.0 0.0 1.0301867251515429 2.2845654756465322E-4 21 0.0 0.0 0.0 1.1470041731396021 2.2845654756465322E-4 22 0.0 0.0 0.0 1.2718176002924244 2.2845654756465322E-4 23 0.0 0.0 0.0 1.4082061591885224 2.2845654756465322E-4 24 0.0 0.0 0.0 1.5362941301897712 3.0460873008620427E-4 25 0.0 0.0 0.0 1.666971275396753 3.0460873008620427E-4 26 0.0 0.0 0.0 1.8018367906424197 3.0460873008620427E-4 27 0.0 0.0 0.0 1.943936763227634 3.0460873008620427E-4 28 0.0 0.0 0.0 2.072329342958969 3.0460873008620427E-4 29 0.0 0.0 0.0 2.205747966736727 3.807609126077553E-4 30 0.0 0.0 0.0 2.3462487434889883 3.807609126077553E-4 31 0.0 0.0 0.0 2.497258521429224 3.807609126077553E-4 32 0.0 0.0 0.0 2.6620518444058607 3.807609126077553E-4 33 0.0 0.0 0.0 2.834003472539523 3.807609126077553E-4 34 0.0 0.0 0.0 3.021642450272625 3.807609126077553E-4 35 0.0 0.0 0.0 3.213774406774498 3.807609126077553E-4 36 0.0 0.0 0.0 3.413140820615919 4.5691309512930643E-4 37 0.0 0.0 0.0 3.611364951719516 4.5691309512930643E-4 38 0.0 0.0 0.0 3.8095129306405924 5.330652776508575E-4 39 0.0 0.0 0.0 4.003015626427853 5.330652776508575E-4 40 0.0 0.0 0.0 4.203219714277012 5.330652776508575E-4 41 0.0 0.0 0.0 4.423223369581772 5.330652776508575E-4 42 0.0 0.0 0.0 4.656172895915197 5.330652776508575E-4 43 0.0 0.0 0.0 4.922933991288191 5.330652776508575E-4 44 0.0 0.0 0.0 5.1954065003503 5.330652776508575E-4 45 0.0 0.0 0.0 5.476331901672302 5.330652776508575E-4 46 0.0 0.0 0.0 5.773858478784002 5.330652776508575E-4 47 0.0 0.0 0.0 6.068110512047276 5.330652776508575E-4 48 0.0 0.0 0.0 6.362591001858113 6.092174601724085E-4 49 0.0 0.0 0.0 6.668570471229706 6.092174601724085E-4 50 0.0 0.0 0.0 6.965487830881233 6.092174601724085E-4 51 0.0 0.0 0.0 7.28936306314539 6.092174601724085E-4 52 0.0 0.0 0.0 7.617274361083188 6.092174601724085E-4 53 0.0 0.0 0.0 7.967726705047367 6.853696426939596E-4 54 0.0 0.0 0.0 8.36364190197691 6.853696426939596E-4 55 0.0 0.0 0.0 8.75734868561333 6.853696426939596E-4 56 0.0 0.0 0.0 9.177251820037162 6.853696426939596E-4 57 0.0 0.0 0.0 9.586798257638064 7.615218252155106E-4 58 0.0 0.0 0.0 10.016905784519784 9.138261902586129E-4 59 0.0 0.0 0.0 10.440235767157088 9.138261902586129E-4 60 0.0 0.0 0.0 10.858768162295531 9.138261902586129E-4 61 0.0 0.0 0.0 11.306923756434859 9.138261902586129E-4 62 0.0 0.0 0.0 11.758353894422614 9.138261902586129E-4 63 0.0 0.0 0.0 12.22844131712815 9.138261902586129E-4 64 0.0 0.0 0.0 12.702793262054891 9.138261902586129E-4 65 0.0 0.0 0.0 13.216211276615187 9.138261902586129E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGCAA 95 8.41938E-6 26.15788 24 ATACCCG 75 0.0016879549 23.666653 21 TGAGCGA 85 0.0034919337 20.882341 20 CGATTGC 395 0.0 20.67954 68 GAACGAT 410 0.0 19.92449 65 TACGTAG 180 1.4710349E-7 19.716953 2 GCATATC 295 0.0 19.250559 4 TCGTGGA 480 0.0 19.232819 46 TTTCGTG 480 0.0 19.231354 44 GTGAACG 410 0.0 19.054577 63 TAGGGCG 205 2.7619535E-8 19.045143 7 TCGTACC 375 0.0 18.936209 49 CGTACCA 380 0.0 18.687048 50 ACCCGTA 190 2.5892768E-7 18.685623 57 CTCGATA 95 0.006653221 18.67993 5 AGTCGTG 115 9.1792905E-4 18.517496 11 TGTTTCG 480 0.0 18.490982 42 AATACGT 135 1.2690268E-4 18.40389 8 CCCGTAA 195 3.4029654E-7 18.20581 58 TAACGCG 215 4.7477442E-8 18.16555 52 >>END_MODULE