FastQCFastQC Report
Thu 26 May 2016
SRR936076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313160
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128050.9751286971884614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96830.737381583356179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74290.5657345639526029No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41840.31862073167016963No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.21840445947180848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.18268908586920102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.1564165828992659No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20340.15489353924883487No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.1367693198087057No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14250.10851686009321028TruSeq Adapter, Index 1 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.1030339029516586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11100.053.094666100-101
CCGTACA3500.052.726913100-101
TTACGCA3200.051.158924100-101
CGAGTAC6500.050.371864100-101
ACGTATC3200.050.228764100-101
CGGTATC8800.047.691956100-101
CGTAGCG255.327454E-447.6116196-97
TCGAGTA2750.046.54194100-101
CACGTAT1900.045.4258998-99
CGTACAT10500.045.356483100-101
TTTACGC2750.044.37212498-99
TCCGGTA1550.044.16265598-99
CCTGTCG5000.043.44559596-97
CCGGTAT3500.043.37214100-101
GCCGGTA559.606083E-943.28987598-99
CCCGGTA2550.043.18377398-99
GGTATCA87950.041.683841
CCCGTAC1800.041.3358298-99
AGGTATC19100.040.98558100-101
CACGCAG6050.040.342903100-101
Produced by FastQC (version 0.11.5)