FastQCFastQC Report
Thu 26 May 2016
SRR936075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670925
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83820.12564974122779074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG5800.017.13753356
CGCGGAA5550.016.62999559
CAATTCG6700.015.89610854
CGATCGT6500.015.8347691
GACGTAT7850.014.92154239
TTACGCG1707.3502003E-414.61730856
CTCGCGC6800.014.09272436
TCGCGCT7350.014.00490137
AATTCGC7000.013.69243655
CGCGCTT7600.013.5438138
CGCTCCG7700.013.368916525
TCGCGGA7500.013.25262858
GCGCGAG4851.4551915E-1113.17495224
TTGCGTA8450.013.02102336
CGTATTA9200.012.7325441
TCGAACT20200.012.47743324
CCTCGCG7700.012.44570935
CGAACTC20050.012.3928925
TGCGTAA8350.012.32766937
GCGGTAT4356.4119376E-912.2391679
Produced by FastQC (version 0.11.5)