FastQCFastQC Report
Thu 26 May 2016
SRR936075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670925
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104320.15638011220333012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.1134475353867717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA25400.046.050106100-101
CGGTATC20600.035.397343100-101
GGTATCA146250.034.7056581
CGTACAT35050.033.286633100-101
GTATCAA282600.029.7731191
TACGCAG19500.029.152147100-101
CCTGTCG18850.028.73227196-97
CGAGTAC22550.028.640787100-101
GCGTATC8850.027.913006100-101
TCGTATC12400.027.602468100-101
CCGGTAT9400.026.279799100-101
ACGTATC10150.026.097311100-101
GCGTACA13800.025.664904100-101
CCGTATC14250.025.48102100-101
CCGTACA18100.024.007454100-101
TCGGTAT8350.023.88142100-101
ATCAACG361050.023.0364383
TCGTACA21550.022.926228100-101
TCAACGC363050.022.8929734
TATCAAC364350.022.8284762
Produced by FastQC (version 0.11.5)