FastQCFastQC Report
Thu 26 May 2016
SRR936074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6704024
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85600.12768450709603665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA5200.023.89710659
ATTCGCG5350.023.88733956
CGATCGT6150.023.660511
CTCGCGC6300.021.97211336
TCGCGCT6600.020.9743237
CGCTCCG7250.019.09514625
TCGCGGA6550.018.970958
AATTCGC6800.018.79455655
CGCGCTT7450.018.58224938
CCTCGCG7500.018.45519835
CGGTTAC5900.018.0464214
ATCGTTC8800.016.5355933
CAATTCG7850.016.28063454
TCGCGGG5250.014.87909163
CTCCGCG9350.014.8049627
GCGCGAG5100.014.6178824
GATCGTT9950.014.2676442
AACTCGA10000.014.19884813
ACGATCG4707.2759576E-1213.59738159
CGCGCCC10000.013.48689330
Produced by FastQC (version 0.11.5)