FastQCFastQC Report
Thu 26 May 2016
SRR936074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6704024
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103430.15428047393625083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76550.11418515208179444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA24250.045.287148100-101
CGGTATC20350.035.1100-101
GGTATCA148700.034.219541
CGTACAT36700.034.141006100-101
CCTGTCG20200.029.46258796-97
GTATCAA286500.028.7702831
CGAGTAC24350.027.989607100-101
TACGCAG18700.027.852024100-101
GCGTATC9000.027.447062100-101
TCGGTAT9700.026.386818100-101
CCGTACA20000.025.892832100-101
ACGTATC10500.025.793625100-101
TCGAGTA12550.025.611814100-101
CCGTATC13600.025.603968100-101
TCGTATC11600.024.887087100-101
CCGGTAT10250.023.8095100-101
TCGTACA22200.023.595001100-101
AGTATCA68900.023.455297100-101
ATCAACG356000.022.7111663
TCAACGC360200.022.446354
Produced by FastQC (version 0.11.5)