FastQCFastQC Report
Thu 26 May 2016
SRR936073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734097
Sequences flagged as poor quality0
Sequence length77
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78880.13756307226752532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC8350.024.65618336
CGATCGT8750.023.5276051
ATTCGCG7850.023.5155956
CGCGGAA8000.022.6311359
TCGCGCT9000.022.48203537
CCTCGCG9850.021.62217535
AATTCGC8950.021.41831855
CAATTCG9100.020.2870254
CGCTCCG10750.019.81194525
TCGCGGA9500.019.80429358
CGGTTAC6850.019.1708984
CGCGCTT10800.019.06238438
AACTCGA11750.018.4280813
ACTCGAG11800.018.34935414
TTGCGTA8250.018.07089636
TGCGTAA8550.017.8527737
GCGTAAG8750.017.84915238
ATCGTTC11350.017.8243663
CGTAAGT8700.017.13589539
GATCGTT12650.016.273472
Produced by FastQC (version 0.11.5)