##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936073_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5734097 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.09780179860927 39.0 36.0 41.0 28.0 41.0 2 35.95127846633916 39.0 35.0 41.0 27.0 41.0 3 35.85656015236575 39.0 35.0 41.0 27.0 41.0 4 35.73250801303152 39.0 35.0 41.0 27.0 41.0 5 35.660700542735846 39.0 35.0 40.0 27.0 41.0 6 35.568904397675865 39.0 35.0 40.0 27.0 41.0 7 35.41349841134532 38.0 35.0 40.0 27.0 41.0 8 35.235452591750715 38.0 34.0 40.0 26.0 41.0 9 35.15348798598977 38.0 34.0 40.0 26.0 41.0 10-11 35.37107490508096 39.0 35.0 40.0 26.5 41.0 12-13 35.573280936824055 39.0 35.0 41.0 27.0 41.0 14-15 35.53006297242617 39.0 35.0 41.0 26.5 41.0 16-17 35.374257010301704 39.0 35.0 41.0 26.0 41.0 18-19 35.23220369310111 39.0 35.0 41.0 25.5 41.0 20-21 35.04892418108727 39.0 34.5 41.0 25.0 41.0 22-23 34.82019060716971 39.0 34.0 41.0 24.0 41.0 24-25 34.61223013841587 38.0 34.0 40.0 23.5 41.0 26-27 33.68893811876569 37.5 33.0 39.5 22.5 40.5 28-29 33.72594769499017 38.0 33.0 39.5 22.0 40.5 30-31 34.09625002855724 38.0 33.0 40.0 22.5 41.0 32-33 33.933895781672334 38.0 33.0 40.0 21.5 41.0 34-35 33.65382378428548 38.0 33.0 40.0 20.0 41.0 36-37 33.35527232971469 37.0 32.5 40.0 18.5 41.0 38-39 33.069719696056765 37.0 32.0 40.0 18.0 41.0 40-41 32.680978016242136 36.0 32.0 39.5 15.0 41.0 42-43 32.312890155154335 36.0 31.5 39.0 13.0 41.0 44-45 31.944120669741025 35.0 31.0 39.0 9.5 40.5 46-47 31.5480044198764 35.0 31.0 38.5 7.5 40.0 48-49 31.074185787230316 35.0 30.5 37.5 4.5 40.0 50-51 30.62936239132334 35.0 30.0 37.0 2.0 39.0 52-53 30.16924661372139 34.5 30.0 36.5 2.0 39.0 54-55 29.705464173347607 34.0 29.0 36.0 2.0 38.5 56-57 29.246257518838625 34.0 29.0 35.5 2.0 37.0 58-59 28.822127703804107 34.0 29.0 35.0 2.0 37.0 60-61 28.470305176211703 34.0 29.0 35.0 2.0 36.0 62-63 28.116644521360556 34.0 27.5 35.0 2.0 36.0 64-65 27.74267116513725 34.0 27.0 35.0 2.0 35.5 66-67 27.35222686327071 33.0 26.0 35.0 2.0 35.0 68-69 26.98377277189416 33.0 25.5 35.0 2.0 35.0 70-71 26.550852557952894 33.0 25.0 35.0 2.0 35.0 72-73 26.049730410908637 33.0 24.5 35.0 2.0 35.0 74-75 25.44036096703631 33.0 21.5 35.0 2.0 35.0 76-77 23.73093854184887 30.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2053953000363435 1101 2 1.080043645562931 1101 3 1.1877776296115883 1101 4 1.2278385083700485 1101 5 1.2446056135025074 1101 6 1.178806988252731 1101 7 1.2679115699655128 1101 8 1.379335880995228 1101 9 1.2997999739116963 1101 10-11 1.2308581810142059 1101 12-13 1.240681858875483 1101 14-15 1.3304284397834962 1101 16-17 1.3448314416355842 1101 18-19 1.3501433589120992 1101 20-21 1.2819165476100238 1101 22-23 1.3206239829500248 1101 24-25 1.3965198212807408 1101 26-27 1.3359760072887426 1101 28-29 1.374528651908328 1101 30-31 1.4368065330585083 1101 32-33 1.5447272195723514 1101 34-35 1.4972638687950663 1101 36-37 1.5323113708371494 1101 38-39 1.5004511144990929 1101 40-41 1.4654063470758416 1101 42-43 1.3770858704662814 1101 44-45 1.2781901426406108 1101 46-47 1.2367644855120439 1101 48-49 1.3356260867097411 1101 50-51 1.3123862025246247 1101 52-53 1.4164639038909854 1101 54-55 1.3045666267604297 1101 56-57 1.3790834595561243 1101 58-59 1.3712822678928838 1101 60-61 1.3341305839747726 1101 62-63 1.1814543591677165 1101 64-65 1.2245765246373352 1101 66-67 1.2715690539671165 1101 68-69 1.2734959404821744 1101 70-71 1.3121279170278584 1101 72-73 1.373436080062426 1101 74-75 1.3471251864588716 1101 76-77 1.3543086924391154 1102 1 1.2157778582699024 1102 2 1.2484709735823358 1102 3 1.2226321764234527 1102 4 1.2562473242553196 1102 5 1.271162510373216 1102 6 1.2176336423954623 1102 7 1.176066307585856 1102 8 1.2718159156769033 1102 9 1.191378745102817 1102 10-11 1.160867940880017 1102 12-13 1.3067507596331964 1102 14-15 1.2384048368830278 1102 16-17 1.2535857632621017 1102 18-19 1.3547064772033366 1102 20-21 1.382489699822301 1102 22-23 1.4271842610582226 1102 24-25 1.400505440860755 1102 26-27 1.2909716798410074 1102 28-29 1.3092706272199308 1102 30-31 1.49100321185351 1102 32-33 1.3853007076504582 1102 34-35 1.4992624115889654 1102 36-37 1.421758946344518 1102 38-39 1.4530131357227063 1102 40-41 1.561109268388833 1102 42-43 1.5415526982826862 1102 44-45 1.4996208026466036 1102 46-47 1.351649720299907 1102 48-49 1.3851965312507453 1102 50-51 1.4662828176603462 1102 52-53 1.4452236854540104 1102 54-55 1.3053156922278752 1102 56-57 1.4643331214676572 1102 58-59 1.4781953815200986 1102 60-61 1.4007858387297105 1102 62-63 1.3914169651647832 1102 64-65 1.3374010532291827 1102 66-67 1.3815797536927654 1102 68-69 1.1824180539023708 1102 70-71 1.3769584889466842 1102 72-73 1.3764085984761252 1102 74-75 1.2746928630703174 1102 76-77 1.5589147676874546 1103 1 0.9237930984924034 1103 2 0.7214113388023264 1103 3 0.7858815909229975 1103 4 0.792849557187516 1103 5 0.9401810321235828 1103 6 0.9434696435034766 1103 7 0.9937831372170223 1103 8 1.0562771924486611 1103 9 0.8665964851260668 1103 10-11 0.893618308638672 1103 12-13 0.9806210271113898 1103 14-15 0.9590760352003116 1103 16-17 0.9623331851190287 1103 18-19 1.043539502530443 1103 20-21 1.0345650105213196 1103 22-23 0.9359541881054909 1103 24-25 0.8827594843607116 1103 26-27 0.7689265825906872 1103 28-29 0.7180764695985715 1103 30-31 1.0508549174822122 1103 32-33 0.9976554751844731 1103 34-35 1.0531266081306043 1103 36-37 0.9647664112402126 1103 38-39 0.9549356048806246 1103 40-41 1.1011350794504153 1103 42-43 1.1071795473225379 1103 44-45 1.0572532168772284 1103 46-47 0.7489039273900922 1103 48-49 0.8293610191391387 1103 50-51 0.9198300749760051 1103 52-53 0.9058181865651846 1103 54-55 1.081764950901512 1103 56-57 1.0721647786693893 1103 58-59 1.0621241809459114 1103 60-61 1.0174129551207187 1103 62-63 0.9567917574921232 1103 64-65 0.9790591257898633 1103 66-67 0.9553109730191522 1103 68-69 0.8538347694745916 1103 70-71 0.9722501029137547 1103 72-73 1.0741277141122794 1103 74-75 1.1012173058291026 1103 76-77 1.1217715833641257 1104 1 0.8158660779729203 1104 2 0.8688012782223709 1104 3 0.7645843213210597 1104 4 0.7654952991077977 1104 5 0.8090028018967956 1104 6 0.6918316572678123 1104 7 0.8475566668896874 1104 8 0.9459972784977708 1104 9 0.9313012455926781 1104 10-11 0.7746898252787489 1104 12-13 0.9664859113278865 1104 14-15 0.8866342139537053 1104 16-17 0.9600741926947265 1104 18-19 0.9178664077766641 1104 20-21 0.933031614893082 1104 22-23 0.918389661609261 1104 24-25 0.9186596548557517 1104 26-27 0.7624900116699322 1104 28-29 0.8198801368211832 1104 30-31 0.9116189037201679 1104 32-33 0.9513987793303755 1104 34-35 0.9995473536479622 1104 36-37 0.9193831945416022 1104 38-39 0.9706273231314597 1104 40-41 0.9589395074709728 1104 42-43 0.9036672605017131 1104 44-45 0.9088177014698005 1104 46-47 0.8524185560898765 1104 48-49 1.0333865388095482 1104 50-51 0.9304722122789748 1104 52-53 0.9610592890842717 1104 54-55 0.8902423372978845 1104 56-57 0.9566717170764782 1104 58-59 0.9061075968293189 1104 60-61 0.8070765754800391 1104 62-63 0.8304914804520145 1104 64-65 0.7985046015142565 1104 66-67 0.8695028081380869 1104 68-69 0.8568060197137761 1104 70-71 0.8666464417438888 1104 72-73 0.7296008190049363 1104 74-75 0.7263392424976765 1104 76-77 0.7299455088073863 1105 1 0.2262222553501374 1105 2 0.3822689835408042 1105 3 0.37369846443250054 1105 4 0.44265157348613116 1105 5 0.4836342882051383 1105 6 0.14911496206337915 1105 7 0.21056309533731365 1105 8 0.42019485571920967 1105 9 0.203353599013127 1105 10-11 0.30263374444394486 1105 12-13 0.3591581918588673 1105 14-15 0.18990886129427054 1105 16-17 0.261768222671499 1105 18-19 0.42003605514593545 1105 20-21 0.4693612479833362 1105 22-23 0.3761673329973618 1105 24-25 0.40377935463478565 1105 26-27 0.2847964289828795 1105 28-29 0.4197835570347621 1105 30-31 0.4240376833782946 1105 32-33 0.5166585164018898 1105 34-35 0.5633976897727351 1105 36-37 0.5315575380452273 1105 38-39 0.5164003282181042 1105 40-41 0.5993024305507646 1105 42-43 0.41885059577237627 1105 44-45 0.2638711800121456 1105 46-47 0.19144484944744633 1105 48-49 0.16239039598685778 1105 50-51 0.3108302472472886 1105 52-53 0.23788339545876624 1105 54-55 0.22624191583163267 1105 56-57 0.3456345917236696 1105 58-59 0.3137038226472946 1105 60-61 -0.006751377470543218 1105 62-63 0.05356722317670304 1105 64-65 0.24923139464775446 1105 66-67 0.2684629512735164 1105 68-69 0.010047295313118099 1105 70-71 -0.20454693464419194 1105 72-73 -0.2014489639266941 1105 74-75 -0.30212855337280686 1105 76-77 -0.14016206872019765 1106 1 0.17886706829184362 1106 2 0.28769352188869135 1106 3 -0.16718408264726747 1106 4 0.09167922922397764 1106 5 -0.06738753522024865 1106 6 0.07427188393923245 1106 7 0.24778312706167327 1106 8 0.3305099877937536 1106 9 0.2604231590893846 1106 10-11 0.017954510650803 1106 12-13 0.1064801169482692 1106 14-15 0.11495176288372733 1106 16-17 0.2258053866304408 1106 18-19 0.22700031830684964 1106 20-21 0.14051341630599268 1106 22-23 0.20488292073418535 1106 24-25 0.3158944567880795 1106 26-27 0.2635704996179058 1106 28-29 0.3807010138125264 1106 30-31 0.3776845270667337 1106 32-33 0.2827083284212648 1106 34-35 0.26730674971896207 1106 36-37 0.32131255774991274 1106 38-39 0.26811069162682344 1106 40-41 0.28569840687602266 1106 42-43 0.3040332820781586 1106 44-45 0.15353509495771078 1106 46-47 0.20290558561348604 1106 48-49 0.14244662482677128 1106 50-51 0.07936207366852344 1106 52-53 0.10745161916187129 1106 54-55 0.28705085488697435 1106 56-57 0.14432285005948842 1106 58-59 0.029861086668450554 1106 60-61 0.02650439301829266 1106 62-63 -0.11776297622858323 1106 64-65 0.052597288364633954 1106 66-67 -0.00724639390752202 1106 68-69 -0.14634160199232227 1106 70-71 -0.3443368528998718 1106 72-73 -0.2299938100425436 1106 74-75 -0.4316054034376613 1106 76-77 -0.3203470386406977 1107 1 0.020823550065216523 1107 2 -0.01605842449246353 1107 3 -0.04049223950254088 1107 4 -0.02663910525276947 1107 5 0.023677758885817468 1107 6 -0.11849456728275243 1107 7 -0.13303790997761666 1107 8 -0.05804218145986795 1107 9 -0.1657703207993535 1107 10-11 0.009048921273823396 1107 12-13 0.11013357210503472 1107 14-15 0.05251428248888601 1107 16-17 0.10103774852291281 1107 18-19 0.13803439623149671 1107 20-21 0.036438390635304074 1107 22-23 -0.0025462360604464607 1107 24-25 -0.3294656356673471 1107 26-27 -0.27394911040988035 1107 28-29 -0.1369014657020955 1107 30-31 0.08537511840593481 1107 32-33 0.14268639404347994 1107 34-35 0.07904400078833618 1107 36-37 0.12501079405125637 1107 38-39 0.08625718309140495 1107 40-41 0.09825797778805168 1107 42-43 0.2013127544365787 1107 44-45 0.12259833267930276 1107 46-47 0.09484178232767704 1107 48-49 0.2615012552887457 1107 50-51 0.14121814976020275 1107 52-53 0.201030077111362 1107 54-55 0.16399384870436862 1107 56-57 0.15025011315953307 1107 58-59 0.11789039658068035 1107 60-61 0.13620026139720665 1107 62-63 0.2613205251071484 1107 64-65 0.25788096629053214 1107 66-67 0.23765173337503498 1107 68-69 0.11472481895316733 1107 70-71 0.07929214126336959 1107 72-73 0.07440065900846093 1107 74-75 -0.06870964251789857 1107 76-77 -0.019439240814406844 1108 1 -0.12171710151616111 1108 2 -0.005430690857963327 1108 3 -0.0022776780030824284 1108 4 -0.19425416060354195 1108 5 -0.14767749391655371 1108 6 -0.09274115501028035 1108 7 -0.20836690951369974 1108 8 0.00901088087774582 1108 9 -0.19843961531230292 1108 10-11 -0.2857325695287116 1108 12-13 -0.20858236630881066 1108 14-15 -0.1059663021290973 1108 16-17 -0.1464229634707621 1108 18-19 -0.09905415981662458 1108 20-21 -0.13672406228801037 1108 22-23 -0.1455791456480071 1108 24-25 -0.03976559253687384 1108 26-27 -0.19682643534240896 1108 28-29 -0.05022496499141482 1108 30-31 -0.10130377226646203 1108 32-33 -0.005635371134069089 1108 34-35 -0.006794716443245363 1108 36-37 -0.09306191117790519 1108 38-39 -0.13457750270088553 1108 40-41 -0.09001885240387253 1108 42-43 -0.0756368429382448 1108 44-45 -0.13490244174240118 1108 46-47 -0.25264420026895706 1108 48-49 -0.1654741129135715 1108 50-51 -0.08464194002824854 1108 52-53 -0.15742694137249202 1108 54-55 -0.04778871813674712 1108 56-57 -0.17745939940684963 1108 58-59 -0.21921685304396377 1108 60-61 -0.2051296244626677 1108 62-63 -0.13897444063302444 1108 64-65 -0.22243890322126347 1108 66-67 -0.2979112674979483 1108 68-69 -0.2434189107506839 1108 70-71 -0.2522145387722574 1108 72-73 -0.25366197503318944 1108 74-75 -0.2535963398057177 1108 76-77 -0.26969417006412755 1109 1 0.08800148084984016 1109 2 0.1544113531326019 1109 3 -0.056650972955686996 1109 4 0.1502018324571921 1109 5 0.11686905244583556 1109 6 0.15924253071695915 1109 7 -0.031202372085964214 1109 8 0.10906688561228606 1109 9 0.21080540811043846 1109 10-11 0.04380919217236823 1109 12-13 0.12713647871792233 1109 14-15 0.25196832379548084 1109 16-17 0.34008578166606895 1109 18-19 0.1424696596816446 1109 20-21 0.18776588271947503 1109 22-23 0.21756103907895152 1109 24-25 0.18696799904872563 1109 26-27 0.05535099807439536 1109 28-29 0.19948482138033086 1109 30-31 0.2730427622004612 1109 32-33 0.25844825570692365 1109 34-35 0.2979193417056294 1109 36-37 0.3410001486683498 1109 38-39 0.3449980705656941 1109 40-41 0.38777423288316015 1109 42-43 0.42506948106515097 1109 44-45 0.2508344158009095 1109 46-47 0.11125425876348416 1109 48-49 0.2930143478699385 1109 50-51 0.2480714684262999 1109 52-53 0.34026554165430767 1109 54-55 0.4116465619152443 1109 56-57 0.36266381224055877 1109 58-59 0.2817012896577715 1109 60-61 0.28371579138726233 1109 62-63 0.34152720256657076 1109 64-65 0.33074487604422487 1109 66-67 0.35280993507196357 1109 68-69 0.31124450547408955 1109 70-71 0.41336932967227114 1109 72-73 0.33864704672861023 1109 74-75 0.20964464236068991 1109 76-77 0.36060008137249966 1110 1 -0.5683766418060543 1110 2 -0.49933211166462854 1110 3 -0.5899611586854263 1110 4 -0.3958895229551018 1110 5 -0.3396908364694937 1110 6 -0.6243238221567466 1110 7 -0.693683868644051 1110 8 -0.5366536713952854 1110 9 -0.42724505346687636 1110 10-11 -0.49836933481220314 1110 12-13 -0.4284028956833552 1110 14-15 -0.5020850727232684 1110 16-17 -0.5928959949561801 1110 18-19 -0.5112047791998933 1110 20-21 -0.7119517006349696 1110 22-23 -0.7551226694649316 1110 24-25 -0.7516947474144402 1110 26-27 -0.5437960494596865 1110 28-29 -0.5295919287080366 1110 30-31 -0.5232165870441747 1110 32-33 -0.35721563930221123 1110 34-35 -0.2571829628606537 1110 36-37 -0.23095636083581184 1110 38-39 -0.19667705559718485 1110 40-41 -0.258212839200084 1110 42-43 -0.1970787406241712 1110 44-45 -0.25000789095109255 1110 46-47 -0.329535218447095 1110 48-49 -0.3437734724804393 1110 50-51 -0.31890646501878095 1110 52-53 -0.2711623270082555 1110 54-55 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-0.9491438849236289 2314 30-31 -1.0080216404248148 2314 32-33 -1.1526928162833627 2314 34-35 -1.0451336610701532 2314 36-37 -1.1446664412039453 2314 38-39 -1.0847041629689258 2314 40-41 -0.8905889173399117 2314 42-43 -0.7589571165794133 2314 44-45 -0.8297043093408689 2314 46-47 -0.836051580182275 2314 48-49 -0.8049600609347465 2314 50-51 -0.7787177137368992 2314 52-53 -0.7416138411890287 2314 54-55 -0.7257148390207568 2314 56-57 -0.5074540755514754 2314 58-59 -0.687747368983036 2314 60-61 -0.6708929228535112 2314 62-63 -0.6113256976127737 2314 64-65 -0.5571338188144104 2314 66-67 -0.5647242152141878 2314 68-69 -0.42196052430376696 2314 70-71 -0.38303766180080956 2314 72-73 -0.30191128893070385 2314 74-75 -0.2920446359220499 2314 76-77 -0.28838983799552054 2315 1 -1.7214263438000614 2315 2 -1.5434333950857138 2315 3 -1.4651374520829492 2315 4 -1.4221127635611523 2315 5 -1.3711041444004408 2315 6 -1.398755293649863 2315 7 -1.5136668765093475 2315 8 -1.5253242943115026 2315 9 -1.5494370388433367 2315 10-11 -1.5090196701494065 2315 12-13 -1.5319152471630844 2315 14-15 -1.8457933486652323 2315 16-17 -1.727956175433249 2315 18-19 -1.5878463867588195 2315 20-21 -1.6500618405626284 2315 22-23 -1.6107580079559725 2315 24-25 -1.6640587137906167 2315 26-27 -1.613880237763432 2315 28-29 -1.5702933691776408 2315 30-31 -1.731079954833774 2315 32-33 -1.7244423169536987 2315 34-35 -1.688554434096318 2315 36-37 -1.9993759635803023 2315 38-39 -1.7143294377142482 2315 40-41 -1.7595047563505553 2315 42-43 -1.9320509996769886 2315 44-45 -1.8991830000483212 2315 46-47 -1.7947788367419584 2315 48-49 -1.715574510030482 2315 50-51 -1.6516983242415186 2315 52-53 -1.6768697533434889 2315 54-55 -1.7728771644833792 2315 56-57 -1.856193575948204 2315 58-59 -1.6720231813627642 2315 60-61 -1.7433109780670648 2315 62-63 -1.8943488537410786 2315 64-65 -1.9490431277786335 2315 66-67 -1.8118824365973545 2315 68-69 -1.8228688026880384 2315 70-71 -1.7922280865274303 2315 72-73 -3.213217001809305 2315 74-75 -3.7847081606878916 2315 76-77 -3.442361880094257 2316 1 -3.9446072057343002 2316 2 -3.8406422935917206 2316 3 -3.3190365925529903 2316 4 -3.170110198996227 2316 5 -3.8875649112320687 2316 6 -3.4331367593254356 2316 7 -3.7735512039455195 2316 8 -3.554955704888993 2316 9 -3.2583045563578494 2316 10-11 -3.2308515308614787 2316 12-13 -3.2502458756818484 2316 14-15 -3.3164418363065735 2316 16-17 -4.638349809435105 2316 18-19 -4.403774405298485 2316 20-21 -3.810499352667506 2316 22-23 -3.8878454235557456 2316 24-25 -3.966922344386404 2316 26-27 -3.6626884497140395 2316 28-29 -3.7195433007224565 2316 30-31 -4.000656801621062 2316 32-33 -4.110047894882406 2316 34-35 -4.207447864402262 2316 36-37 -4.078476495861096 2316 38-39 -4.167232338210461 2316 40-41 -4.068618578954791 2316 42-43 -4.280666869258177 2316 44-45 -4.049807979177835 2316 46-47 -3.647398700098737 2316 48-49 -3.842619516809215 2316 50-51 -4.209591240298767 2316 52-53 -5.265452964793205 2316 54-55 -4.573553835624676 2316 56-57 -4.406766863065943 2316 58-59 -4.415765976872105 2316 60-61 -4.132239337213008 2316 62-63 -4.1772773998865524 2316 64-65 -4.049562251819427 2316 66-67 -4.229203067320043 2316 68-69 -4.186603183218718 2316 70-71 -4.006651359954134 2316 72-73 -3.9935733694770867 2316 74-75 -4.006792070046551 2316 76-77 -3.9134207649092296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 180648.0 3 13747.0 4 16673.0 5 19007.0 6 22002.0 7 23817.0 8 25999.0 9 26435.0 10 29761.0 11 32338.0 12 28916.0 13 27294.0 14 27519.0 15 29099.0 16 30216.0 17 31865.0 18 34044.0 19 35521.0 20 38452.0 21 41123.0 22 46242.0 23 52058.0 24 60532.0 25 70302.0 26 82532.0 27 98051.0 28 114182.0 29 135493.0 30 159825.0 31 190842.0 32 231199.0 33 290189.0 34 380326.0 35 504774.0 36 702061.0 37 916804.0 38 790046.0 39 192912.0 40 1251.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.1804741003573 26.451534249088787 27.7295373864099 22.63845426414402 2 22.962750172586947 26.62877951463562 27.538458372097807 22.870011940679625 3 23.137535341836887 26.739132330651337 27.247793151924988 22.87553917558679 4 22.71659017683593 26.72869575652373 27.661399589480546 22.893314477159795 5 22.996823857521843 26.8027704473983 27.43901056446732 22.761395130612534 6 23.15740105749824 26.600217806406246 27.512308294433517 22.730072841662 7 23.025426574280395 26.419305184300146 27.563906561373063 22.991361680046396 8 22.943671899406095 26.76935276050131 27.375537473720456 22.911437866372143 9 22.940523399664578 26.59716959164291 27.407517231094197 23.054789777598312 10-11 23.295232591514736 26.681040388612043 27.173730364593723 22.8499966552795 12-13 23.31528246385342 26.4457345827106 27.20735784057262 23.031625112863356 14-15 23.02189303115938 26.618135634694195 27.37412863112875 22.985842703017674 16-17 23.283400316860803 26.478866877302593 27.378060563526684 22.859672242309916 18-19 23.222315810992235 26.45965485802754 27.218282055544286 23.099747275435938 20-21 23.13178272127036 26.63802045471057 27.132620704518196 23.097576119500875 22-23 23.17586751992325 26.704329150631768 26.887516216599256 23.232287112845725 24-25 23.200527887887663 26.712027162573825 26.895777987071597 23.19166696246692 26-27 23.18124634993333 26.739516241008715 26.871389617448592 23.207847791609364 28-29 23.34198494997175 26.72219363348801 26.833275472758395 23.102545943781845 30-31 23.2480662939582 26.564281571424097 26.82360315643823 23.364048978179472 32-33 23.10373783257738 26.822288981950194 26.92005654839067 23.153916637081753 34-35 23.21822190207258 26.583859923291993 26.96427792787215 23.233640246763283 36-37 23.251371428930227 26.599599262989944 27.11300867403072 23.036020634049112 38-39 23.119607694367193 26.585413074703247 27.11988851068803 23.17509072024154 40-41 23.08186844217533 26.630493363644515 26.999313498509963 23.28832469567019 42-43 22.99637100768985 26.77984752860147 26.85133375845416 23.37244770525452 44-45 22.953417069637492 26.54374039774788 27.10608124683704 23.396761285777586 46-47 22.97725618169936 26.50621944277205 27.155859665850222 23.360664709678368 48-49 23.113876279965062 26.449597907487977 27.047645378868783 23.388880433678175 50-51 22.93626573311652 26.666200868547758 26.94522615370954 23.45230724462618 52-53 22.975850649538707 26.551828935325965 27.000841254771096 23.471479160364233 54-55 23.001345481803025 26.47140811918809 27.03874160731085 23.48850479169803 56-57 22.854489758664847 26.726025020933925 27.01435978649273 23.4051254339085 58-59 22.91303321763856 26.605899439212628 27.072446067973686 23.408621275175125 60-61 22.973550239648407 26.44594907153031 26.93017351179543 23.650327177025847 62-63 22.890992296849568 26.36810747970247 27.121545524852404 23.61935469859556 64-65 22.922246515679745 26.338282849109717 27.074892694204912 23.664577941005625 66-67 22.87146459382351 26.42028976658709 27.190706745433314 23.517538894156086 68-69 22.841240983680493 26.210712434811683 27.28526903475277 23.66277754675506 70-71 22.751636367601993 26.12510332944371 27.242540104910738 23.880720198043555 72-73 22.818383881913476 26.051375820444083 27.22092726839388 23.909313029248562 74-75 22.718757846215958 25.98657997622561 27.196174663999777 24.098487513558652 76-77 22.561152287164834 25.870404872842972 27.493577478182278 24.074865361809916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7669.0 1 4739.0 2 1695.0 3 1652.5 4 1724.0 5 1814.5 6 1928.0 7 2062.0 8 2258.0 9 2343.0 10 2445.5 11 2568.0 12 2767.5 13 2947.0 14 3153.0 15 3571.0 16 4024.0 17 4265.0 18 4681.5 19 5762.5 20 7191.5 21 9395.5 22 10835.0 23 12701.0 24 17413.0 25 24701.5 26 29144.0 27 34709.5 28 48701.5 29 63748.5 30 70369.0 31 82161.5 32 105418.5 33 128552.5 34 150296.0 35 160370.0 36 172312.0 37 194069.0 38 210772.5 39 217661.0 40 224026.0 41 239048.0 42 257891.0 43 268077.0 44 271922.0 45 278937.0 46 285388.0 47 290623.0 48 292577.0 49 292962.5 50 290234.0 51 279534.5 52 271949.0 53 266601.0 54 249262.5 55 223912.0 56 196475.5 57 182399.0 58 170777.0 59 143793.0 60 117293.0 61 106155.0 62 93720.0 63 69435.0 64 50004.0 65 42423.0 66 37162.5 67 28470.5 68 20649.0 69 13854.5 70 11450.0 71 9615.0 72 6390.5 73 4481.5 74 3962.0 75 3297.0 76 2174.5 77 1400.5 78 1084.0 79 838.0 80 523.0 81 382.0 82 262.5 83 215.0 84 203.0 85 178.5 86 142.5 87 119.0 88 112.5 89 103.0 90 90.0 91 80.0 92 87.5 93 84.0 94 80.5 95 76.5 96 65.0 97 73.5 98 91.0 99 192.0 100 284.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009469668894683854 2 0.013166850857249189 3 0.014073706810331254 4 0.015224716289243101 5 0.012678543805589616 6 0.009853338720987804 7 0.016079253629647353 8 0.01789296553581148 9 0.01747441663438899 10-11 0.020604813626278036 12-13 0.01652396183740875 14-15 0.00777803375143462 16-17 0.006086398608185386 18-19 0.0170558677329665 20-21 0.02241852553244216 22-23 0.020221143799974084 24-25 0.018512069119165583 26-27 0.023107387266033345 28-29 0.008484335022585074 30-31 0.008414576872347992 32-33 0.010472442304341904 34-35 0.011632171552033388 36-37 0.011527534326677767 38-39 0.014160904498127604 40-41 0.011841446002744635 42-43 0.01438761848639812 44-45 0.012931417100199037 46-47 0.022819634896305382 48-49 0.02222669061929019 50-51 0.0348790751185409 52-53 0.03807051049188739 54-55 0.02751959026852877 56-57 0.020535055476040953 58-59 0.015835100103817567 60-61 0.01944508437858655 62-63 0.025156532929247626 64-65 0.024676945646367684 66-67 0.027066162291987737 68-69 0.04076491904479467 70-71 0.05292027672360618 72-73 0.08382313727863341 74-75 0.08410216987958175 76-77 0.08787782976116379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 5734097.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.619687834369 #Duplication Level Percentage of deduplicated Percentage of total 1 68.96437671419324 30.77168960679129 2 14.17688279332939 12.651361694055893 3 6.087645713635088 8.148865541658964 4 3.249748848174907 5.800111165826583 5 1.966609271754833 4.387474589893821 6 1.2461334791646006 3.336125210416839 7 0.8618866964361406 2.691998074050331 8 0.61221329323988 2.185341282593157 9 0.4414195476426296 1.7726402177822256 >10 2.182113680344738 16.97733416207162 >50 0.13712954121078033 4.226675955829576 >100 0.07030465919058512 5.6443183743659295 >500 0.0026714705699663307 0.7803917985667909 >1k 8.250051536425727E-4 0.48740142403933884 >5k 3.928595968289904E-5 0.1382709020577614 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7888 0.13756307226752532 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.7439537559270447E-5 0.0 2 0.0 0.0 0.0 1.9183491315197493E-4 0.0 3 0.0 0.0 0.0 2.7903260094832715E-4 0.0 4 0.0 0.0 0.0 7.150210399300884E-4 0.0 5 0.0 0.0 0.0 0.001831151443723397 0.0 6 0.0 0.0 0.0 0.0038541378005987692 0.0 7 0.0 0.0 0.0 0.008684889704516684 0.0 8 0.0 0.0 0.0 0.013341246232841893 0.0 9 0.0 0.0 0.0 0.018921898251808438 0.0 10 0.0 0.0 0.0 0.025915152813075885 0.0 11 0.0 0.0 0.0 0.033518791188917804 0.0 12 0.0 0.0 0.0 0.04436618355078402 0.0 13 0.0 0.0 0.0 0.05755047394559248 0.0 14 1.7439537559270447E-5 0.0 0.0 0.07436218815272919 0.0 15 1.7439537559270447E-5 0.0 0.0 0.09382471206887501 0.0 16 1.7439537559270447E-5 0.0 0.0 0.11250245679485366 0.0 17 1.7439537559270447E-5 0.0 0.0 0.13193010163588095 0.0 18 1.7439537559270447E-5 0.0 0.0 0.1504857695989447 0.0 19 1.7439537559270447E-5 0.0 0.0 0.17102954484376529 0.0 20 1.7439537559270447E-5 0.0 0.0 0.1946252391614582 0.0 21 1.7439537559270447E-5 0.0 0.0 0.2202962384487043 0.0 22 1.7439537559270447E-5 0.0 0.0 0.24812974039329994 0.0 23 1.7439537559270447E-5 0.0 0.0 0.27687009829097764 0.0 24 1.7439537559270447E-5 0.0 0.0 0.30613364231543344 0.0 25 1.7439537559270447E-5 0.0 0.0 0.33682722841974944 0.0 26 1.7439537559270447E-5 0.0 0.0 0.3690031752166034 0.0 27 1.7439537559270447E-5 0.0 0.0 0.4016499895275577 0.0 28 1.7439537559270447E-5 0.0 0.0 0.4342619247633934 0.0 29 1.7439537559270447E-5 0.0 0.0 0.4686526928302748 0.0 30 1.7439537559270447E-5 0.0 0.0 0.5056593915310467 0.0 31 1.7439537559270447E-5 0.0 0.0 0.5451425045652349 0.0 32 1.7439537559270447E-5 0.0 0.0 0.5879042506605661 0.0 33 1.7439537559270447E-5 0.0 0.0 0.6361071324743897 0.0 34 1.7439537559270447E-5 0.0 0.0 0.6841181793750611 0.0 35 1.7439537559270447E-5 0.0 0.0 0.7329314450034591 0.0 36 1.7439537559270447E-5 0.0 0.0 0.7858255624207264 0.0 37 1.7439537559270447E-5 0.0 0.0 0.8407775452699876 0.0 38 1.7439537559270447E-5 0.0 0.0 0.8960085607201971 0.0 39 1.7439537559270447E-5 0.0 0.0 0.9541868580179234 0.0 40 1.7439537559270447E-5 0.0 0.0 1.017038951381534 0.0 41 1.7439537559270447E-5 0.0 0.0 1.0856984107523817 0.0 42 1.7439537559270447E-5 0.0 0.0 1.1575144264214574 0.0 43 1.7439537559270447E-5 0.0 0.0 1.2352773243982444 0.0 44 1.7439537559270447E-5 0.0 0.0 1.3189871046827426 0.0 45 3.487907511854089E-5 0.0 0.0 1.4044408387231677 0.0 46 3.487907511854089E-5 0.0 0.0 1.497829562353061 0.0 47 5.231861267781135E-5 0.0 0.0 1.5918809884102065 0.0 48 5.231861267781135E-5 0.0 0.0 1.6898563104181878 0.0 49 5.231861267781135E-5 0.0 0.0 1.7920345609779536 0.0 50 5.231861267781135E-5 0.0 0.0 1.8961660397443574 0.0 51 5.231861267781135E-5 0.0 0.0 2.0090870454406335 0.0 52 5.231861267781135E-5 0.0 0.0 2.1303615896278 0.0 53 5.231861267781135E-5 0.0 0.0 2.257373741671967 0.0 54 5.231861267781135E-5 0.0 0.0 2.395250725615559 0.0 55 5.231861267781135E-5 0.0 0.0 2.538743240653236 0.0 56 5.231861267781135E-5 0.0 0.0 2.692699478226476 0.0 57 5.231861267781135E-5 0.0 0.0 2.8476497694405936 0.0 58 5.231861267781135E-5 0.0 0.0 3.0103955339437056 0.0 59 5.231861267781135E-5 0.0 0.0 3.17858243416531 0.0 60 5.231861267781135E-5 0.0 0.0 3.3474320368141663 0.0 61 5.231861267781135E-5 0.0 0.0 3.527652217951667 0.0 62 5.231861267781135E-5 0.0 0.0 3.716801442319514 0.0 63 5.231861267781135E-5 0.0 0.0 3.9168329381243465 0.0 64 5.231861267781135E-5 0.0 0.0 4.1251307747322725 0.0 65 5.231861267781135E-5 0.0 0.0 4.348287097340697 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGC 835 0.0 24.656183 36 CGATCGT 875 0.0 23.527605 1 ATTCGCG 785 0.0 23.51559 56 CGCGGAA 800 0.0 22.63113 59 TCGCGCT 900 0.0 22.482035 37 CCTCGCG 985 0.0 21.622175 35 AATTCGC 895 0.0 21.418318 55 CAATTCG 910 0.0 20.28702 54 CGCTCCG 1075 0.0 19.811945 25 TCGCGGA 950 0.0 19.804293 58 CGGTTAC 685 0.0 19.170898 4 CGCGCTT 1080 0.0 19.062384 38 AACTCGA 1175 0.0 18.42808 13 ACTCGAG 1180 0.0 18.349354 14 TTGCGTA 825 0.0 18.070896 36 TGCGTAA 855 0.0 17.85277 37 GCGTAAG 875 0.0 17.849152 38 ATCGTTC 1135 0.0 17.824366 3 CGTAAGT 870 0.0 17.135895 39 GATCGTT 1265 0.0 16.27347 2 >>END_MODULE