FastQCFastQC Report
Thu 26 May 2016
SRR936073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734097
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82100.14317860336161037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60160.10491625795657103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA19600.043.88293100-101
GGTATCA122850.033.8099671
CGTACAT30450.033.13342100-101
CGGTATC17950.031.170748100-101
GTATCAA226550.030.0487521
ACGTATC8550.027.846954100-101
CGAGTAC21550.026.515987100-101
CCTGTCG20350.026.1742596-97
TACGCAG16850.026.140606100-101
TCGAGTA9950.025.125229100-101
TCGTATC9900.024.350264100-101
ACCGTAC6550.024.08092998-99
CGTCCTA7200.023.974485100-101
CCGTACA16500.023.448402100-101
GCGTACA12150.023.270256100-101
TATCAAC294150.022.9183772
TCAACGC292150.022.8700124
GCGTATC6250.022.856781100-101
ATCAACG291850.022.8531483
CCGGTAT6850.022.59262100-101
Produced by FastQC (version 0.11.5)