FastQCFastQC Report
Thu 26 May 2016
SRR936072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5765685
Sequences flagged as poor quality0
Sequence length77
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79020.13705223230197278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG8000.028.3997156
CGCGGAA8250.026.68268459
AATTCGC9400.025.30337155
TCGCGGA8800.024.61069358
CTCGCGC9500.024.2841736
TCGCGCT9650.023.90918537
CGGTTAC6650.023.4848184
CCTCGCG10250.022.85275335
CGATCGT10450.022.4159621
CGCGCTT10900.021.81974438
CAATTCG11150.021.33236154
CGCTCCG11500.020.68024625
AACTCGA12150.019.28191413
ACTCGAG12850.018.78303114
GCGTAAG6950.018.38734638
TGCGTAA7250.018.11517337
ATCGTTC13350.017.5470333
TTCGCGG13750.016.52547557
GCGCCCT14100.016.360431
AAACTCG14450.016.21465112
Produced by FastQC (version 0.11.5)