FastQCFastQC Report
Thu 26 May 2016
SRR936072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5765685
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82930.1438337335459707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.1042200536449702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA18400.046.10294100-101
GGTATCA124300.035.238981
CGTACAT32600.033.690727100-101
CGGTATC15750.033.260876100-101
GTATCAA224600.029.6773381
ACGTATC7350.028.752302100-101
GCGTACA13450.027.662365100-101
TACGCAG19050.026.87417100-101
CGAGTAC24150.025.266108100-101
GCGTATC6900.025.019606100-101
TCGAGTA10550.024.82743100-101
TCGTATC11400.024.542828100-101
AGCGTAC8550.024.01730398-99
TCGTACA19500.023.353846100-101
CCGTATC10600.023.025528100-101
TATCAAC293000.022.5800932
ATACACG13850.022.5602196-97
ATCAACG292100.022.4661563
TCAACGC293050.022.3933264
CCGTACA15700.022.181341100-101
Produced by FastQC (version 0.11.5)