FastQCFastQC Report
Thu 26 May 2016
SRR936071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6514245
Sequences flagged as poor quality0
Sequence length77
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG3750.023.6624310
CGTTCGG3950.023.36272411
CGCGGAA7400.022.54724159
CGCGCTT8750.021.90515338
CGATCGT9100.021.8419061
TCGCGCT8950.021.81374537
ATTCGCG8000.021.74336256
CTCGCGC8900.021.53579736
AATTCGC8850.021.25885655
CGGTTAC7300.019.4472274
CGCTCCG10150.019.23566625
CCTCGCG9900.019.00179535
TCGCGGA9500.018.68359258
CAATTCG10200.017.05492654
ACGCTCC12150.015.77810224
GATCGTT13300.015.7445752
ATCGTTC13200.015.32562353
AACTCGA13250.015.26954913
GATCGCG6750.015.25310261
GCGCGAG8700.015.09801224
Produced by FastQC (version 0.11.5)