##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936071_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6514245 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.58038345195798 39.0 35.0 41.0 27.0 41.0 2 35.433465428457176 39.0 35.0 41.0 27.0 41.0 3 35.33657438429166 39.0 34.0 40.0 26.0 41.0 4 35.20379568161775 39.0 34.0 40.0 25.0 41.0 5 35.13129810131489 39.0 34.0 40.0 25.0 41.0 6 35.03525228173027 38.0 34.0 40.0 25.0 41.0 7 34.86993826606153 38.0 34.0 40.0 25.0 41.0 8 34.68662400631232 38.0 34.0 40.0 24.0 41.0 9 34.60272203455658 38.0 34.0 40.0 24.0 41.0 10-11 34.82173559637379 38.0 34.0 40.0 24.5 41.0 12-13 35.011934844329616 39.0 34.0 40.5 25.0 41.0 14-15 34.959134251168024 39.0 34.0 41.0 24.0 41.0 16-17 34.8019716022348 39.0 34.0 41.0 24.0 41.0 18-19 34.65833047421459 39.0 34.0 41.0 24.0 41.0 20-21 34.47081073861975 38.5 33.5 41.0 23.0 41.0 22-23 34.238415349745054 38.0 33.0 40.0 21.5 41.0 24-25 34.029727389743556 38.0 33.0 40.0 19.5 41.0 26-27 33.1239115968159 37.5 32.0 39.5 16.5 40.5 28-29 33.166563661636914 37.5 32.5 39.5 16.5 40.5 30-31 33.53198037531594 38.0 33.0 40.0 17.0 41.0 32-33 33.36438627960723 38.0 33.0 40.0 14.5 41.0 34-35 33.08353830720214 37.5 32.0 40.0 12.0 41.0 36-37 32.781960841202626 37.0 32.0 40.0 8.5 41.0 38-39 32.49200743908158 37.0 31.5 40.0 8.0 41.0 40-41 32.09672540716537 36.0 31.0 39.5 6.5 41.0 42-43 31.72845203089537 36.0 31.0 39.0 2.0 41.0 44-45 31.348534941501278 35.0 31.0 39.0 2.0 40.5 46-47 30.94659987458255 35.0 30.0 38.0 2.0 40.0 48-49 30.464861392225806 35.0 30.0 37.5 2.0 39.5 50-51 30.012121281898363 35.0 29.0 37.0 2.0 39.0 52-53 29.544947572588995 34.0 29.0 36.0 2.0 39.0 54-55 29.072516461999818 34.0 29.0 36.0 2.0 38.5 56-57 28.611960173435293 34.0 28.0 35.0 2.0 37.0 58-59 28.178581477976344 34.0 27.5 35.0 2.0 37.0 60-61 27.81299697816094 34.0 26.5 35.0 2.0 36.0 62-63 27.45931140139801 34.0 26.0 35.0 2.0 36.0 64-65 27.07196421074123 33.0 26.0 35.0 2.0 35.5 66-67 26.675037936092366 33.0 25.0 35.0 2.0 35.0 68-69 26.290226265668547 33.0 24.5 35.0 2.0 35.0 70-71 25.84811348053381 33.0 23.5 35.0 2.0 35.0 72-73 25.33875099877269 32.5 19.5 35.0 2.0 35.0 74-75 24.720633626767185 32.0 10.0 35.0 2.0 35.0 76-77 23.023621693688217 30.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2072555264207168 1101 2 1.1756192014524132 1101 3 1.3028087588268562 1101 4 1.2902898866357475 1101 5 1.2867722554049834 1101 6 1.20404014817138 1101 7 1.344977724876621 1101 8 1.469490569909297 1101 9 1.519164762889801 1101 10-11 1.3867488794702751 1101 12-13 1.4171851414219887 1101 14-15 1.4484144252694549 1101 16-17 1.476203209286254 1101 18-19 1.481873646258073 1101 20-21 1.3858574561787051 1101 22-23 1.4793543447540927 1101 24-25 1.4917942219237261 1101 26-27 1.327753759211305 1101 28-29 1.4295947896173473 1101 30-31 1.5764647267348835 1101 32-33 1.6458971813483316 1101 34-35 1.5810641562196608 1101 36-37 1.6444870454025846 1101 38-39 1.6585101733012948 1101 40-41 1.5807472706358396 1101 42-43 1.5301236754818603 1101 44-45 1.5024367529373386 1101 46-47 1.5249672454522596 1101 48-49 1.5752195379915044 1101 50-51 1.5105882524451317 1101 52-53 1.5726998066697497 1101 54-55 1.6088787356923682 1101 56-57 1.5741409260805739 1101 58-59 1.5758993132469215 1101 60-61 1.568082013935033 1101 62-63 1.5128522229516612 1101 64-65 1.4361105701620822 1101 66-67 1.4284489150278965 1101 68-69 1.4804510741281263 1101 70-71 1.5083801110802533 1101 72-73 1.5751427185614872 1101 74-75 1.49143350476702 1101 76-77 1.5525371763638276 1102 1 1.1133001297414538 1102 2 1.2475789300858153 1102 3 1.2510068752385095 1102 4 1.3172860993799844 1102 5 1.284344609505922 1102 6 1.2505667347923008 1102 7 1.1382329171337844 1102 8 1.2556096794235785 1102 9 1.262208644218184 1102 10-11 1.1886440827068512 1102 12-13 1.3545640528369418 1102 14-15 1.3249485255238582 1102 16-17 1.3111923099785585 1102 18-19 1.385929833497606 1102 20-21 1.4030334781289824 1102 22-23 1.4419553275608479 1102 24-25 1.4914647328080832 1102 26-27 1.414700169435406 1102 28-29 1.4136316560850801 1102 30-31 1.440764073737654 1102 32-33 1.4722078217640941 1102 34-35 1.483030196946018 1102 36-37 1.5156410476540145 1102 38-39 1.4796768851814832 1102 40-41 1.5457458608282835 1102 42-43 1.565501968503952 1102 44-45 1.6186644389945535 1102 46-47 1.4243513090730247 1102 48-49 1.508169437489311 1102 50-51 1.5188990413179049 1102 52-53 1.5040523247783 1102 54-55 1.4780341755202855 1102 56-57 1.566583903636861 1102 58-59 1.4915738634435485 1102 60-61 1.4675217489339332 1102 62-63 1.4646799263110601 1102 64-65 1.3705387650316645 1102 66-67 1.4468567830357308 1102 68-69 1.4089447267899935 1102 70-71 1.5596447725448748 1102 72-73 1.62940388861632 1102 74-75 1.5626582911064126 1102 76-77 1.6716328061739922 1103 1 0.9892586877622023 1103 2 0.8424862593757467 1103 3 0.8522581572271335 1103 4 0.8851704031069403 1103 5 0.9223097376698774 1103 6 0.7970551866155233 1103 7 0.9327326380442855 1103 8 0.999496014355806 1103 9 0.893102979651573 1103 10-11 0.9624650216733315 1103 12-13 0.940007285372225 1103 14-15 0.9719585585879074 1103 16-17 0.9266812053582001 1103 18-19 0.9445786068226241 1103 20-21 1.016041781284187 1103 22-23 0.9941909455297946 1103 24-25 0.8059394631831793 1103 26-27 0.6931399160671319 1103 28-29 0.7800367927089908 1103 30-31 1.0965911726497737 1103 32-33 1.076054051883574 1103 34-35 1.0648248166472953 1103 36-37 1.0226645537202756 1103 38-39 0.9987106342592682 1103 40-41 1.0178403892581755 1103 42-43 1.1412505984634649 1103 44-45 1.0937847684636886 1103 46-47 0.8202168497037263 1103 48-49 0.8536701592624816 1103 50-51 0.9175608226575811 1103 52-53 0.9410058674307002 1103 54-55 0.9944487452337043 1103 56-57 1.020251360830855 1103 58-59 0.9462949610973332 1103 60-61 1.0706281445831642 1103 62-63 0.9517787763614471 1103 64-65 0.9321261724123495 1103 66-67 0.9468102721588068 1103 68-69 0.8642714840647407 1103 70-71 0.8608566178676149 1103 72-73 0.8138877000674078 1103 74-75 0.8457115967169848 1103 76-77 0.9894635926943813 1104 1 0.7239087852202246 1104 2 0.7121779244475732 1104 3 0.6395511502029265 1104 4 0.5855413661505224 1104 5 0.6997584521155673 1104 6 0.5229680725565871 1104 7 0.7881173138080584 1104 8 0.846571442675355 1104 9 0.7779195887119386 1104 10-11 0.7018810652004888 1104 12-13 0.8088661522459475 1104 14-15 0.7371881277588983 1104 16-17 0.8203394109316022 1104 18-19 0.8548227620314321 1104 20-21 0.7954421420825426 1104 22-23 0.8549727170944905 1104 24-25 0.9347887259647507 1104 26-27 0.6928959707812083 1104 28-29 0.676651896646284 1104 30-31 0.931501257441326 1104 32-33 0.9084721190289216 1104 34-35 0.9309285897445747 1104 36-37 0.7551379982349218 1104 38-39 0.8857482947133732 1104 40-41 0.8967766235240475 1104 42-43 0.857389820666981 1104 44-45 0.8800045607044034 1104 46-47 0.7323426369385686 1104 48-49 0.7910337934513443 1104 50-51 0.7543552655904513 1104 52-53 0.7827412201933832 1104 54-55 0.8229666541058407 1104 56-57 0.7475773832754058 1104 58-59 0.7088526490595015 1104 60-61 0.4807086728279266 1104 62-63 0.5662491020080509 1104 64-65 0.5301598002333847 1104 66-67 0.4904837559871531 1104 68-69 0.3778983736589474 1104 70-71 0.3078512931279569 1104 72-73 0.3429914427090601 1104 74-75 0.34948073332577323 1104 76-77 0.22383960147712756 1105 1 0.4198461462450851 1105 2 0.6979996337757939 1105 3 0.5908818043120405 1105 4 0.6594154730219941 1105 5 0.642187166911782 1105 6 0.4599664023566845 1105 7 0.45896536138209854 1105 8 0.7144961933586558 1105 9 0.5242808876972305 1105 10-11 0.6830046171037978 1105 12-13 0.7124129566444637 1105 14-15 0.5310429243634971 1105 16-17 0.5906226979879037 1105 18-19 0.695400720719249 1105 20-21 0.6788614024217665 1105 22-23 0.6566272984679813 1105 24-25 0.6493136293763584 1105 26-27 0.5605226951845452 1105 28-29 0.6983705677751644 1105 30-31 0.6680622469440323 1105 32-33 0.8136127041884365 1105 34-35 0.8343109369972623 1105 36-37 0.8851175366006743 1105 38-39 0.7827835380781494 1105 40-41 0.9151164999997334 1105 42-43 0.7137780782881151 1105 44-45 0.5850527495887086 1105 46-47 0.5182496516873059 1105 48-49 0.48050789779821557 1105 50-51 0.6395268109611862 1105 52-53 0.6178656637370068 1105 54-55 0.6068182175102024 1105 56-57 0.6345233158191448 1105 58-59 0.6855910237275431 1105 60-61 0.46123264586967494 1105 62-63 0.43790778775757744 1105 64-65 0.5771301739921917 1105 66-67 0.5989226357124409 1105 68-69 0.4598856399565925 1105 70-71 0.2067300391779625 1105 72-73 0.10535962895592732 1105 74-75 -0.01183107813992379 1105 76-77 0.16018200331074794 1106 1 0.16077763713299476 1106 2 0.22626318357826136 1106 3 -0.13307100578135334 1106 4 0.09951924712215998 1106 5 -0.03969984676091087 1106 6 0.18326831216424466 1106 7 0.23447693128396452 1106 8 0.24206398333444668 1106 9 0.20905966680404475 1106 10-11 -0.003737495154290116 1106 12-13 0.12356142643162826 1106 14-15 0.10994663292212437 1106 16-17 0.31046303152150756 1106 18-19 0.2628776264304591 1106 20-21 0.10315288020136393 1106 22-23 0.20674607429771186 1106 24-25 0.31014662589901576 1106 26-27 0.2817826948204214 1106 28-29 0.2931711576489988 1106 30-31 0.2582448893603342 1106 32-33 0.1820308956061396 1106 34-35 0.24535082630488603 1106 36-37 0.30052726223652115 1106 38-39 0.18017097959143058 1106 40-41 0.20903621161696861 1106 42-43 0.18425798798643456 1106 44-45 -0.03944503838441804 1106 46-47 0.15785494598701177 1106 48-49 0.05561707907518709 1106 50-51 0.04401509541145998 1106 52-53 -0.011394008191189187 1106 54-55 0.17706836770955903 1106 56-57 0.09780301432807192 1106 58-59 -0.04443118757108877 1106 60-61 -0.09355443516515649 1106 62-63 -0.10560079636316289 1106 64-65 -0.17758051474012504 1106 66-67 -0.11916701054532197 1106 68-69 -0.31118443486999325 1106 70-71 -0.45342019658951216 1106 72-73 -0.46882352322787924 1106 74-75 -0.5690589988346666 1106 76-77 -0.5753593879904315 1107 1 0.13691228922392185 1107 2 0.020819826131528885 1107 3 0.13462156334272635 1107 4 0.15333712215132778 1107 5 0.0935509625038975 1107 6 -0.1040264038301757 1107 7 -0.07649869802787634 1107 8 -0.008588610202210134 1107 9 -0.02271463657003636 1107 10-11 0.1537196123148803 1107 12-13 0.1487796616659196 1107 14-15 0.1792212324781488 1107 16-17 0.2187398509462497 1107 18-19 0.22152461943578317 1107 20-21 0.14209268047302004 1107 22-23 0.20074151489080094 1107 24-25 -0.07925618753029795 1107 26-27 0.01325667568526967 1107 28-29 0.08378660578126329 1107 30-31 0.30161213702228906 1107 32-33 0.3653617996491718 1107 34-35 0.3548723997191203 1107 36-37 0.33143214295597545 1107 38-39 0.31734480548431776 1107 40-41 0.2190365227575981 1107 42-43 0.3568494902571615 1107 44-45 0.3872696157408875 1107 46-47 0.2750227285715141 1107 48-49 0.4236188262494913 1107 50-51 0.35150952778923283 1107 52-53 0.4523114134342805 1107 54-55 0.4260937795221338 1107 56-57 0.4765421528275411 1107 58-59 0.4156541367630986 1107 60-61 0.44416861377367667 1107 62-63 0.5072035141312519 1107 64-65 0.5106529484345863 1107 66-67 0.5428834660383579 1107 68-69 0.3362313985979668 1107 70-71 0.34763392392961734 1107 72-73 0.4615480385185755 1107 74-75 0.3255266037310456 1107 76-77 0.3607343132068621 1108 1 -0.04799477437090616 1108 2 0.08189701244233305 1108 3 -0.04885316193281142 1108 4 -0.06876156943662437 1108 5 -0.0037884969731152296 1108 6 -0.021240164019452834 1108 7 -0.16824016571202804 1108 8 0.042023890392790975 1108 9 -0.07544003682589562 1108 10-11 -0.26233675541462986 1108 12-13 -0.13866299683223104 1108 14-15 -0.056806135174234385 1108 16-17 0.0076251103796494135 1108 18-19 -0.03719537544260021 1108 20-21 -0.0503889316866335 1108 22-23 -0.01765345625210557 1108 24-25 -0.028614997734713654 1108 26-27 -0.11806996184078855 1108 28-29 0.01794592825857677 1108 30-31 0.005482139520374574 1108 32-33 0.027012022760750654 1108 34-35 -0.008164134455718397 1108 36-37 0.0178252564039596 1108 38-39 -0.04983856352448157 1108 40-41 0.070120084343813 1108 42-43 0.047647987275148296 1108 44-45 -0.05935126689758974 1108 46-47 -0.0990365470067971 1108 48-49 -0.0776877316036213 1108 50-51 -0.05500399082037433 1108 52-53 -0.08530049751001201 1108 54-55 0.05426435130251406 1108 56-57 -0.0014068986576774023 1108 58-59 -0.041809972216750424 1108 60-61 -0.14933922308274106 1108 62-63 -0.12133547403108835 1108 64-65 -0.11649878773251388 1108 66-67 -0.24311910528890124 1108 68-69 -0.23257960285254242 1108 70-71 -0.3356958563092647 1108 72-73 -0.3046811470512907 1108 74-75 -0.283097858876868 1108 76-77 -0.30281998262731946 1109 1 0.3008961871043212 1109 2 0.30064167473825876 1109 3 0.012754195238692034 1109 4 0.2879774630158636 1109 5 0.18446804615854973 1109 6 0.1836817728814495 1109 7 -0.02602106156594175 1109 8 0.1626295726068676 1109 9 0.3005252646175336 1109 10-11 0.15957917090229756 1109 12-13 0.32094930056074844 1109 14-15 0.38703731462106106 1109 16-17 0.5257500722867832 1109 18-19 0.25988533779129597 1109 20-21 0.3231120905189684 1109 22-23 0.35446550033820046 1109 24-25 0.33809806384818586 1109 26-27 0.2371862235000961 1109 28-29 0.4019456735536764 1109 30-31 0.5051714240065479 1109 32-33 0.407508023856316 1109 34-35 0.540464324846802 1109 36-37 0.5649084621340279 1109 38-39 0.5557697692006158 1109 40-41 0.5866498016702906 1109 42-43 0.5904276867309051 1109 44-45 0.4962567930637789 1109 46-47 0.3342038240572194 1109 48-49 0.4466410062565096 1109 50-51 0.48640869205360104 1109 52-53 0.5269969895271274 1109 54-55 0.532959810783364 1109 56-57 0.5138486358125114 1109 58-59 0.54287075125049 1109 60-61 0.407641496847976 1109 62-63 0.36386930381131677 1109 64-65 0.4233050594024128 1109 66-67 0.4647432716758466 1109 68-69 0.4551113787883878 1109 70-71 0.40816456127131673 1109 72-73 0.36614363027944563 1109 74-75 0.326118996943606 1109 76-77 0.37408436910170195 1110 1 -0.40394758158334554 1110 2 -0.5113755031603873 1110 3 -0.4893575832975756 1110 4 -0.4523864530766346 1110 5 -0.3779648635313535 1110 6 -0.5161119083473622 1110 7 -0.6112750062932051 1110 8 -0.44472819454608725 1110 9 -0.4462031693710671 1110 10-11 -0.43805526656834815 1110 12-13 -0.4787493390536568 1110 14-15 -0.5327116616596683 1110 16-17 -0.5448158482304848 1110 18-19 -0.4205816209576412 1110 20-21 -0.6039770184753905 1110 22-23 -0.7252812488244729 1110 24-25 -0.6527628312290759 1110 26-27 -0.5555557178112664 1110 28-29 -0.5160266109626903 1110 30-31 -0.5470313106239004 1110 32-33 -0.34199148833626936 1110 34-35 -0.1561468310876819 1110 36-37 -0.18499866407861987 1110 38-39 -0.20376792870971627 1110 40-41 -0.1906406048889977 1110 42-43 -0.3133959016176391 1110 44-45 -0.3211772542721114 1110 46-47 -0.36576116414618554 1110 48-49 -0.3523828508655278 1110 50-51 -0.3701762540179985 1110 52-53 -0.4478215800238523 1110 54-55 -0.3801977437372521 1110 56-57 -0.386992789120594 1110 58-59 -0.38072662758720455 1110 60-61 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-0.7063502064488674 2314 56-57 -0.6091535747304597 2314 58-59 -0.6647180132703667 2314 60-61 -0.6802376756794892 2314 62-63 -0.6539152960265078 2314 64-65 -0.598396242449251 2314 66-67 -0.7192538596878677 2314 68-69 -0.5569971013240362 2314 70-71 -0.5511114487055373 2314 72-73 -0.46357830388336296 2314 74-75 -0.5009700016342293 2314 76-77 -0.4100925354131739 2315 1 -1.6907271574855827 2315 2 -1.6345342974579466 2315 3 -1.6377602858729574 2315 4 -1.5878258222924657 2315 5 -1.4744718519133357 2315 6 -1.5770614430955234 2315 7 -1.72526888236996 2315 8 -1.5642471659427883 2315 9 -1.4532652222756752 2315 10-11 -1.6629325717250651 2315 12-13 -1.712521584875077 2315 14-15 -2.0085969480138957 2315 16-17 -1.8390317444910949 2315 18-19 -1.7376124265250255 2315 20-21 -1.839747935599405 2315 22-23 -1.8698739342896573 2315 24-25 -1.9903462895153297 2315 26-27 -1.9440779034334739 2315 28-29 -1.838569503578995 2315 30-31 -2.047905389729401 2315 32-33 -1.9430637086589257 2315 34-35 -1.87835434043334 2315 36-37 -2.1124196391517707 2315 38-39 -1.9526184865599916 2315 40-41 -1.9815989680023236 2315 42-43 -2.1741647971860267 2315 44-45 -2.103100398338622 2315 46-47 -1.990577857791429 2315 48-49 -1.8428990222558568 2315 50-51 -1.7917038111292598 2315 52-53 -1.8741425411810262 2315 54-55 -1.9068417510824816 2315 56-57 -1.951929918261019 2315 58-59 -1.8084100053755847 2315 60-61 -1.8906433145125128 2315 62-63 -2.0164100134806695 2315 64-65 -2.079746823599436 2315 66-67 -1.9565707603664144 2315 68-69 -1.9532631524884287 2315 70-71 -1.9780041381953524 2315 72-73 -3.295265773120537 2315 74-75 -3.804160186755091 2315 76-77 -3.4279725266765624 2316 1 -4.132340016490222 2316 2 -4.141421567992268 2316 3 -3.517084502174221 2316 4 -3.4467825705583763 2316 5 -4.113915907205847 2316 6 -3.6970045302837455 2316 7 -4.089944870379451 2316 8 -3.9704943314544785 2316 9 -3.4999759844166043 2316 10-11 -3.495402986794538 2316 12-13 -3.626094349906353 2316 14-15 -3.7231862500938178 2316 16-17 -5.024979244484982 2316 18-19 -4.7976551003529195 2316 20-21 -3.997334512881075 2316 22-23 -4.162951005203652 2316 24-25 -4.241821996300452 2316 26-27 -4.047846600754518 2316 28-29 -4.1297945171378245 2316 30-31 -4.272254411531765 2316 32-33 -4.461088215312415 2316 34-35 -4.363777146544784 2316 36-37 -4.393468680968347 2316 38-39 -4.460802979459885 2316 40-41 -4.481550238162139 2316 42-43 -4.6395441743247545 2316 44-45 -4.38544607555858 2316 46-47 -4.035491713064957 2316 48-49 -4.261101984579739 2316 50-51 -4.647982370732684 2316 52-53 -5.6564193401853835 2316 54-55 -4.891787392873887 2316 56-57 -4.738987679987815 2316 58-59 -4.762842801295925 2316 60-61 -4.484217964051769 2316 62-63 -4.5233884907512625 2316 64-65 -4.4130599845997125 2316 66-67 -4.56226317234449 2316 68-69 -4.536639537701216 2316 70-71 -4.241222358900703 2316 72-73 -4.204286358914548 2316 74-75 -4.12981083194153 2316 76-77 -4.1083855602269885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 264087.0 3 18272.0 4 21889.0 5 25138.0 6 28652.0 7 30373.0 8 33576.0 9 33565.0 10 36950.0 11 39723.0 12 36122.0 13 34233.0 14 35060.0 15 36996.0 16 38376.0 17 40078.0 18 41798.0 19 44018.0 20 46941.0 21 50800.0 22 56000.0 23 62871.0 24 72446.0 25 83612.0 26 97592.0 27 113179.0 28 132962.0 29 155416.0 30 180715.0 31 215517.0 32 261205.0 33 322498.0 34 418899.0 35 554910.0 36 772222.0 37 1011047.0 38 859538.0 39 205630.0 40 1339.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.101033543769667 26.694141450615167 27.695521791375565 22.5093032142396 2 22.818570204944464 26.75567055909005 27.63548444496849 22.790274790996992 3 23.07780764443003 26.942304751622654 27.22981989733159 22.75006770661572 4 22.600368077569268 26.856735163936072 27.639471905259715 22.90342485323494 5 22.99588145290477 26.864438604232284 27.49553156217078 22.644148380692172 6 23.083164430994348 26.765307065526944 27.456412142791127 22.69511636068758 7 22.993965861101497 26.4123542169422 27.615957811693477 22.977722110262828 8 22.925662550490053 26.839290397109604 27.39564058267226 22.839406469728075 9 22.84376647619153 26.649323387970185 27.554932409422978 22.951977726415308 10-11 23.263969736827054 26.751533874911843 27.240548266670107 22.743948121591 12-13 23.241934139211878 26.52622979437369 27.28047235600562 22.95136371040881 14-15 22.946813531914653 26.796388236830232 27.401252868633787 22.85554536262133 16-17 23.18548671849046 26.566258327617327 27.48651031921433 22.761744634677893 18-19 23.13630784641212 26.503646669633014 27.314002345439153 23.046043138515714 20-21 23.10749469152823 26.695636205120977 27.260186745810454 22.936682357540334 22-23 23.119813213302525 26.775958759280737 26.955134388631397 23.149093638785338 24-25 23.155937510915706 26.86513963851403 26.923223671508158 23.05569917906211 26-27 23.147575690131657 26.805911260764276 26.937423769001086 23.10908928010299 28-29 23.285583331299165 26.770206627030312 26.906611079357607 23.037598962312916 30-31 23.202647767667397 26.639627303227257 26.96250763869181 23.195217290413524 32-33 23.08877698952851 27.033997068590885 26.944261621584776 22.932964320295824 34-35 23.173678017336705 26.728606023638672 27.01790421663612 23.079811742388507 36-37 23.185140804715715 26.74097083115678 27.20300819408179 22.870880170045716 38-39 23.101929672687294 26.749264607498574 27.17194977061373 22.9768559492004 40-41 23.006513771573918 26.742795644282285 27.09876360777968 23.151926976364116 42-43 22.94331631394639 26.94794011323211 26.879932678858054 23.228810893963445 44-45 22.901424301091446 26.71471143202367 27.20451931949206 23.179344947392824 46-47 22.92593596052117 26.633118646168008 27.1443848280298 23.296560565281023 48-49 23.07807584867239 26.66058985086287 27.062049699273622 23.199284601191113 50-51 22.9432472917506 26.788056560061225 26.99291198687574 23.275784161312437 52-53 23.029648451573212 26.688535999628975 26.988687757094688 23.293127791703128 54-55 22.974545118964162 26.674860722421514 27.049305372199335 23.301288786414993 56-57 22.861669902030133 26.945252368330586 26.990009551781757 23.203068177857524 58-59 23.016646169860945 26.832260897468373 27.002630039565744 23.148462893104934 60-61 22.958314482985863 26.712282652262893 26.89726876026373 23.432134104487517 62-63 22.942697822839566 26.658985133177076 27.103455100840474 23.294861943142884 64-65 22.992728189559045 26.57526718429321 27.0440700150141 23.387934611133648 66-67 22.8965618162585 26.76761837110961 27.118593837836958 23.217225974794932 68-69 22.91023190927084 26.52332515090197 27.260627024875326 23.305815914951857 70-71 22.82170961268446 26.477890497811018 27.188523583620032 23.51187630588448 72-73 22.887618324186665 26.403168273872886 27.148836983127616 23.56037641881283 74-75 22.77136944550671 26.30062377211046 27.139126270410024 23.788880511972803 76-77 22.67288483972686 26.13252145227452 27.49073208731716 23.703861620681458 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5252.0 1 3326.0 2 1406.5 3 1503.5 4 1594.0 5 1643.5 6 1852.5 7 2115.5 8 2253.0 9 2287.0 10 2434.5 11 2732.0 12 3054.0 13 3226.0 14 3445.5 15 3909.0 16 4498.5 17 4844.0 18 5279.0 19 6541.0 20 8504.0 21 11393.5 22 13147.0 23 15609.5 24 21410.5 25 29870.5 26 34992.0 27 41645.0 28 56960.0 29 74365.0 30 83108.0 31 96041.0 32 121755.0 33 147878.5 34 174304.5 35 187388.0 36 201959.0 37 228296.5 38 247089.5 39 254116.0 40 261247.5 41 278719.5 42 300877.5 43 312695.0 44 314596.5 45 318771.5 46 324665.5 47 329690.0 48 331094.0 49 331700.5 50 328548.5 51 314721.5 52 304653.0 53 299002.0 54 280046.5 55 249334.5 56 216692.0 57 201457.0 58 188880.5 59 158482.5 60 128138.0 61 115615.0 62 101096.5 63 73732.5 64 52866.0 65 44845.0 66 38492.5 67 28641.5 68 21240.0 69 14666.5 70 11996.0 71 10327.5 72 7080.0 73 4801.5 74 4102.0 75 3475.5 76 2368.5 77 1516.0 78 1144.0 79 938.5 80 599.0 81 401.0 82 296.5 83 256.0 84 240.5 85 187.5 86 140.0 87 130.0 88 128.5 89 126.0 90 102.5 91 80.0 92 82.0 93 89.5 94 95.0 95 84.0 96 73.0 97 83.5 98 109.5 99 241.5 100 358.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00976321891485506 2 0.012618499918256068 3 0.01303297619294331 4 0.014491318640916944 5 0.01201981196593005 6 0.009502252371533463 7 0.014905794915604186 8 0.01659440196062629 9 0.01625668055162187 10-11 0.019134988014727725 12-13 0.01568869454556898 14-15 0.00726868578016332 16-17 0.0057873168724848385 18-19 0.018098797328009616 20-21 0.023671200576582552 22-23 0.020900349925432646 24-25 0.01945735845059558 26-27 0.02389378968706274 28-29 0.008105313816106088 30-31 0.008159042222084063 32-33 0.010039536431313223 34-35 0.011106429064304459 36-37 0.010784058628436604 38-39 0.013608637685564483 40-41 0.011321342688216362 42-43 0.015143734999220938 44-45 0.01343210149449399 46-47 0.02441572277370593 48-49 0.023241373328758745 50-51 0.03585219776044653 52-53 0.03897612079373742 54-55 0.02763942713238449 56-57 0.020324688432811478 58-59 0.01588825719634432 60-61 0.020317012946243194 62-63 0.025428887000719193 64-65 0.026081303359023186 66-67 0.028000175001093756 68-69 0.04195420958223094 70-71 0.05482600055724033 72-73 0.08581961531996418 74-75 0.0862417670812197 76-77 0.09060911893857232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 6514245.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.32723011105824 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34746373226703 34.24005064008443 2 13.599797538933489 12.872814951778174 3 5.462920771320563 7.756347252683042 4 2.6716421546218267 5.057656921047626 5 1.5899158511243585 3.762315667169077 6 0.9894191140875342 2.8095879653220073 7 0.6377104048841117 2.1126746953316533 8 0.4337299121245388 1.6421788285733696 9 0.33387084245917253 1.422106396959432 >10 1.7045317089334366 14.521927249222328 >50 0.13737435146587754 4.528423257464625 >100 0.08702084401835622 7.4709715916118995 >500 0.0036887709962906525 1.1562646065844462 >1k 8.813598077351384E-4 0.552101156642789 >5k 3.264295575023461E-5 0.09457881952530836 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.140389254625824E-5 0.0 3 0.0 0.0 0.0 1.2280778509251647E-4 0.0 4 0.0 0.0 0.0 4.298272478238077E-4 0.0 5 0.0 1.535097313656456E-5 0.0 8.596544956476154E-4 0.0 6 1.535097313656456E-5 1.535097313656456E-5 0.0 0.0017346599644317952 0.0 7 1.535097313656456E-5 1.535097313656456E-5 0.0 0.0038223923110045754 0.0 8 1.535097313656456E-5 1.535097313656456E-5 0.0 0.006309249959128034 0.0 9 1.535097313656456E-5 1.535097313656456E-5 0.0 0.008995670258026832 0.0 10 1.535097313656456E-5 1.535097313656456E-5 0.0 0.012265427536115084 0.0 11 1.535097313656456E-5 1.535097313656456E-5 0.0 0.016072468873983094 0.0 12 1.535097313656456E-5 1.535097313656456E-5 0.0 0.021552766283736643 0.0 13 1.535097313656456E-5 1.535097313656456E-5 0.0 0.028537459060873516 0.0 14 1.535097313656456E-5 1.535097313656456E-5 0.0 0.03748707639949066 1.535097313656456E-5 15 1.535097313656456E-5 1.535097313656456E-5 0.0 0.04846302219213432 3.070194627312912E-5 16 1.535097313656456E-5 1.535097313656456E-5 0.0 0.059039842683227296 3.070194627312912E-5 17 3.070194627312912E-5 1.535097313656456E-5 0.0 0.06977017290568592 3.070194627312912E-5 18 3.070194627312912E-5 1.535097313656456E-5 0.0 0.08119129691928996 3.070194627312912E-5 19 3.070194627312912E-5 1.535097313656456E-5 0.0 0.09408611435400419 3.070194627312912E-5 20 3.070194627312912E-5 1.535097313656456E-5 0.0 0.10713444152008406 3.070194627312912E-5 21 3.070194627312912E-5 1.535097313656456E-5 0.0 0.1219327796237323 4.605291940969368E-5 22 3.070194627312912E-5 1.535097313656456E-5 0.0 0.1383583208798564 4.605291940969368E-5 23 3.070194627312912E-5 1.535097313656456E-5 0.0 0.15539790106144305 4.605291940969368E-5 24 3.070194627312912E-5 1.535097313656456E-5 0.0 0.172744500705761 4.605291940969368E-5 25 3.070194627312912E-5 1.535097313656456E-5 0.0 0.19122707236218472 4.605291940969368E-5 26 3.070194627312912E-5 1.535097313656456E-5 0.0 0.21110658257403583 6.140389254625824E-5 27 3.070194627312912E-5 1.535097313656456E-5 0.0 0.23158478073821295 6.140389254625824E-5 28 3.070194627312912E-5 1.535097313656456E-5 0.0 0.25172525749338565 6.140389254625824E-5 29 3.070194627312912E-5 1.535097313656456E-5 0.0 0.27220345565756277 7.67548656828228E-5 30 3.070194627312912E-5 1.535097313656456E-5 0.0 0.29288121647251525 7.67548656828228E-5 31 3.070194627312912E-5 1.535097313656456E-5 0.0 0.3169515423506485 1.0745681195595192E-4 32 3.070194627312912E-5 1.535097313656456E-5 0.0 0.34158985423483457 1.0745681195595192E-4 33 3.070194627312912E-5 1.535097313656456E-5 0.0 0.37066459735548785 1.0745681195595192E-4 34 3.070194627312912E-5 1.535097313656456E-5 0.0 0.4015661062793923 1.0745681195595192E-4 35 3.070194627312912E-5 1.535097313656456E-5 0.0 0.43334262067208096 1.0745681195595192E-4 36 3.070194627312912E-5 1.535097313656456E-5 0.0 0.46643931875451417 1.0745681195595192E-4 37 3.070194627312912E-5 1.535097313656456E-5 0.0 0.5011018160968769 1.0745681195595192E-4 38 3.070194627312912E-5 1.535097313656456E-5 0.0 0.536316948472156 1.0745681195595192E-4 39 3.070194627312912E-5 1.535097313656456E-5 0.0 0.5733281447044132 1.2280778509251647E-4 40 3.070194627312912E-5 1.535097313656456E-5 0.0 0.6115060148950492 1.2280778509251647E-4 41 3.070194627312912E-5 1.535097313656456E-5 0.0 0.6545347925968397 1.2280778509251647E-4 42 3.070194627312912E-5 1.535097313656456E-5 0.0 0.7000657789198902 1.2280778509251647E-4 43 3.070194627312912E-5 1.535097313656456E-5 0.0 0.7498796867480422 1.2280778509251647E-4 44 3.070194627312912E-5 1.535097313656456E-5 0.0 0.8030861596393749 1.2280778509251647E-4 45 3.070194627312912E-5 1.535097313656456E-5 0.0 0.8582268551459148 1.2280778509251647E-4 46 3.070194627312912E-5 1.535097313656456E-5 0.0 0.9215649703073802 1.2280778509251647E-4 47 3.070194627312912E-5 1.535097313656456E-5 0.0 0.9829074589610922 1.2280778509251647E-4 48 3.070194627312912E-5 1.535097313656456E-5 0.0 1.0485482200930423 1.535097313656456E-4 49 3.070194627312912E-5 1.535097313656456E-5 0.0 1.112577129045653 1.535097313656456E-4 50 3.070194627312912E-5 1.535097313656456E-5 0.0 1.1811959789660966 1.535097313656456E-4 51 3.070194627312912E-5 1.535097313656456E-5 0.0 1.2551569675380647 1.535097313656456E-4 52 3.070194627312912E-5 1.535097313656456E-5 0.0 1.3344600947615572 1.535097313656456E-4 53 3.070194627312912E-5 1.535097313656456E-5 0.0 1.4201645777829972 1.535097313656456E-4 54 3.070194627312912E-5 1.535097313656456E-5 0.0 1.5124392773068867 1.535097313656456E-4 55 3.070194627312912E-5 1.535097313656456E-5 0.0 1.60612626635934 1.535097313656456E-4 56 3.070194627312912E-5 1.535097313656456E-5 0.0 1.7079339202010364 1.535097313656456E-4 57 3.070194627312912E-5 1.535097313656456E-5 0.0 1.8131187881327766 1.6886070450221016E-4 58 3.070194627312912E-5 1.535097313656456E-5 0.0 1.9252729978685175 1.6886070450221016E-4 59 3.070194627312912E-5 1.535097313656456E-5 0.0 2.0373043998191656 1.6886070450221016E-4 60 3.070194627312912E-5 1.535097313656456E-5 0.0 2.1539871466301928 1.6886070450221016E-4 61 3.070194627312912E-5 1.535097313656456E-5 0.0 2.2761501908509736 1.6886070450221016E-4 62 3.070194627312912E-5 1.535097313656456E-5 0.0 2.4034097581530935 1.6886070450221016E-4 63 3.070194627312912E-5 1.535097313656456E-5 0.0 2.539158413599734 1.8421167763877473E-4 64 3.070194627312912E-5 1.535097313656456E-5 0.0 2.6828742241042516 1.8421167763877473E-4 65 3.070194627312912E-5 1.535097313656456E-5 0.0 2.836491412281853 1.8421167763877473E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTCG 375 0.0 23.66243 10 CGTTCGG 395 0.0 23.362724 11 CGCGGAA 740 0.0 22.547241 59 CGCGCTT 875 0.0 21.905153 38 CGATCGT 910 0.0 21.841906 1 TCGCGCT 895 0.0 21.813745 37 ATTCGCG 800 0.0 21.743362 56 CTCGCGC 890 0.0 21.535797 36 AATTCGC 885 0.0 21.258856 55 CGGTTAC 730 0.0 19.447227 4 CGCTCCG 1015 0.0 19.235666 25 CCTCGCG 990 0.0 19.001795 35 TCGCGGA 950 0.0 18.683592 58 CAATTCG 1020 0.0 17.054926 54 ACGCTCC 1215 0.0 15.778102 24 GATCGTT 1330 0.0 15.744575 2 ATCGTTC 1320 0.0 15.3256235 3 AACTCGA 1325 0.0 15.269549 13 GATCGCG 675 0.0 15.253102 61 GCGCGAG 870 0.0 15.098012 24 >>END_MODULE