FastQCFastQC Report
Thu 26 May 2016
SRR936071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6514245
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79500.12204023643568826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA19950.044.00893100-101
CGGTATC18050.038.4185100-101
GGTATCA123750.033.94581
CGTACAT32600.030.766209100-101
GTATCAA233200.029.7761751
GCGTATC7600.027.803944100-101
ACGTATC9350.025.146477100-101
CCGTACA17900.024.275126100-101
CCGGTAT8150.024.101717100-101
CCTGTCG16300.023.55078596-97
TCGTATC11350.023.33758100-101
CGAGTAC22050.023.215687100-101
TCAACGC299900.022.7737924
ATCAACG299900.022.7541283
AGCGTAT8250.022.36631498-99
TATCAAC308100.022.303872
CAACGCA312100.021.8832265
TCGTACA17800.021.73627100-101
TCGAGTA11250.021.693169100-101
GCGTACA13350.021.624804100-101
Produced by FastQC (version 0.11.5)