FastQCFastQC Report
Thu 26 May 2016
SRR936070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6561431
Sequences flagged as poor quality0
Sequence length77
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG2800.022.8199210
CGCGGAA7700.021.20978459
CGATCGT9100.020.672251
CGGTTAC7950.019.1989634
ATTCGCG8900.019.14567656
TCGCGGA8800.018.9620158
CGTTCGG3350.018.01359611
CTCGCGC9950.017.8365236
CCTCGCG10350.017.8321235
CGCGCTT10000.017.74950238
CAATTCG10400.016.7261354
CGCTCCG10450.016.64318325
AATTCGC10500.016.5669655
TCGCGCT11150.016.23647137
CGCCTAG4007.2759576E-1215.084661526
ACTCGAG12650.014.5900314
CCGCCTA4151.4551915E-1114.53976625
AACTCGA12750.014.47703313
ATCGTTC13050.013.8714823
GATCGTT13250.013.6621022
Produced by FastQC (version 0.11.5)