##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936070_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6561431 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5693561358795 39.0 35.0 41.0 27.0 41.0 2 35.433392807148316 39.0 35.0 41.0 27.0 41.0 3 35.29018456492189 39.0 34.0 40.0 26.0 41.0 4 35.17508192953641 39.0 34.0 40.0 25.0 41.0 5 35.10097827745198 39.0 34.0 40.0 25.0 41.0 6 35.01123977376277 38.0 34.0 40.0 25.0 41.0 7 34.87143719106396 38.0 34.0 40.0 25.0 41.0 8 34.745963952070824 38.0 34.0 40.0 24.0 41.0 9 34.92359791636916 38.0 34.0 40.0 25.0 41.0 10-11 35.149218516509585 39.0 35.0 40.5 25.0 41.0 12-13 35.07072054861203 39.0 34.5 41.0 25.0 41.0 14-15 34.92272935583716 39.0 34.0 41.0 24.0 41.0 16-17 34.77425457952694 39.0 34.0 41.0 24.0 41.0 18-19 34.62372857384311 39.0 34.0 41.0 23.5 41.0 20-21 34.43786766941541 38.0 33.5 40.5 22.5 41.0 22-23 34.19929996977793 38.0 33.0 40.0 21.5 41.0 24-25 33.99472881753995 38.0 33.0 40.0 19.5 41.0 26-27 33.07158324152155 37.5 32.0 39.5 16.5 40.5 28-29 33.094957258561436 37.5 32.5 39.5 15.5 40.5 30-31 33.48996026933759 38.0 33.0 40.0 16.0 41.0 32-33 33.359624295370935 38.0 33.0 40.0 13.5 41.0 34-35 33.09538612842229 37.5 32.0 40.0 12.5 41.0 36-37 32.77734102515137 37.0 32.0 40.0 8.0 41.0 38-39 32.4568315204412 37.0 31.5 40.0 7.5 41.0 40-41 32.09179278117838 36.0 31.0 39.5 6.5 41.0 42-43 31.707326420105616 36.0 31.0 39.0 2.0 41.0 44-45 31.33311582488637 35.0 31.0 39.0 2.0 40.0 46-47 30.901336690121408 35.0 30.0 38.0 2.0 40.0 48-49 30.468492543775895 35.0 30.0 37.5 2.0 39.5 50-51 30.001552252854598 35.0 29.0 37.0 2.0 39.0 52-53 29.5064669581986 34.0 29.0 36.0 2.0 39.0 54-55 29.033133625881305 34.0 29.0 36.0 2.0 38.0 56-57 28.581695669740334 34.0 28.0 35.0 2.0 37.0 58-59 28.150090429968706 34.0 27.0 35.0 2.0 37.0 60-61 27.775944073785123 34.0 26.5 35.0 2.0 36.0 62-63 27.394253250548545 33.5 26.0 35.0 2.0 36.0 64-65 27.00707932461684 33.0 25.5 35.0 2.0 35.5 66-67 26.636475869364475 33.0 25.0 35.0 2.0 35.0 68-69 26.247022410202895 33.0 24.5 35.0 2.0 35.0 70-71 25.772648679838284 33.0 23.5 35.0 2.0 35.0 72-73 25.265759557633082 32.0 19.5 35.0 2.0 35.0 74-75 24.657940851622154 32.0 9.5 34.5 2.0 35.0 76-77 22.906165743417862 30.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3937966688559555 1101 2 1.3514959254213608 1101 3 1.3676026215377775 1101 4 1.300753055571306 1101 5 1.2661448461772267 1101 6 1.4126163566897318 1101 7 1.4050898841159878 1101 8 1.3216793937381155 1101 9 1.3341220453805107 1101 10-11 1.3652314965197476 1101 12-13 1.5065281022895718 1101 14-15 1.4138165151474809 1101 16-17 1.4657946373565807 1101 18-19 1.6029229476659097 1101 20-21 1.525642537743927 1101 22-23 1.4911900700269314 1101 24-25 1.5322365499054484 1101 26-27 1.5469731531393833 1101 28-29 1.6182958487113908 1101 30-31 1.5847155726509357 1101 32-33 1.6910317549621396 1101 34-35 1.6612810250354713 1101 36-37 1.64137432494757 1101 38-39 1.6030877219865758 1101 40-41 1.497845925596451 1101 42-43 1.5557387023647529 1101 44-45 1.490421741486383 1101 46-47 1.542125378379378 1101 48-49 1.5059255122356383 1101 50-51 1.549078122027339 1101 52-53 1.6751144891792684 1101 54-55 1.615618694534927 1101 56-57 1.5664704210918643 1101 58-59 1.6737928602185868 1101 60-61 1.6343507232292858 1101 62-63 1.619679731664828 1101 64-65 1.6204295226663845 1101 66-67 1.6678452995348323 1101 68-69 1.7403252792384585 1101 70-71 1.8068545059249423 1101 72-73 1.7396199757804105 1101 74-75 1.7933427751850637 1101 76-77 1.9433569504128627 1102 1 1.161228707406913 1102 2 1.1876305980749606 1102 3 1.2616143365280479 1102 4 1.2158165004938652 1102 5 1.2247575391404908 1102 6 1.2201306320153336 1102 7 1.1850443084828441 1102 8 1.2325160213713175 1102 9 1.2786753216306153 1102 10-11 1.371828408900008 1102 12-13 1.4303365291570955 1102 14-15 1.4204096679787739 1102 16-17 1.4339108808190417 1102 18-19 1.4153502817129393 1102 20-21 1.4415490173253787 1102 22-23 1.306023515408178 1102 24-25 1.4375141835213512 1102 26-27 1.2884198492642511 1102 28-29 1.440315277117584 1102 30-31 1.5569650689538719 1102 32-33 1.5505953121054716 1102 34-35 1.4661898386486243 1102 36-37 1.4689112273366263 1102 38-39 1.6095667565966352 1102 40-41 1.6406462761985523 1102 42-43 1.5953014260431821 1102 44-45 1.4464246592735073 1102 46-47 1.5734472768068706 1102 48-49 1.5041826725500194 1102 50-51 1.4939824859373445 1102 52-53 1.4824057136471573 1102 54-55 1.5006540326451905 1102 56-57 1.6189177790387816 1102 58-59 1.7445989325006437 1102 60-61 1.5943504776211341 1102 62-63 1.5092429574021473 1102 64-65 1.525969440802971 1102 66-67 1.5740159615610025 1102 68-69 1.5206443209273068 1102 70-71 1.4190824165241445 1102 72-73 1.3963130265452577 1102 74-75 1.3725764362677815 1102 76-77 1.4296365177333286 1103 1 0.69505885842689 1103 2 0.7367287634432103 1103 3 0.7327785131206639 1103 4 0.6456665660625021 1103 5 0.6545182737602815 1103 6 0.6583989598238915 1103 7 0.6724194604622653 1103 8 0.5727282329329455 1103 9 0.6829636488324695 1103 10-11 0.7768580923213975 1103 12-13 0.8293923477504208 1103 14-15 0.7566923173306321 1103 16-17 0.6542240946980868 1103 18-19 0.6692410652718124 1103 20-21 0.7576252873131253 1103 22-23 0.6173934762901467 1103 24-25 0.6657091625548475 1103 26-27 0.8026349532146284 1103 28-29 0.7028957211712026 1103 30-31 0.8464944496658475 1103 32-33 0.8963823513540206 1103 34-35 0.937048423433005 1103 36-37 0.9097919449502925 1103 38-39 0.922490036932281 1103 40-41 0.9908048136442602 1103 42-43 0.9105472529361727 1103 44-45 0.8638193336566253 1103 46-47 0.8464474101175696 1103 48-49 0.8644827482942041 1103 50-51 0.7990361815549818 1103 52-53 0.9036254983486494 1103 54-55 0.9158490689778738 1103 56-57 0.9271877142465286 1103 58-59 0.8373986557908815 1103 60-61 0.72130333108014 1103 62-63 0.6859473032261221 1103 64-65 0.6097358246812803 1103 66-67 0.6746835959215005 1103 68-69 0.6203187034587963 1103 70-71 0.5747762765962641 1103 72-73 0.6775460656071495 1103 74-75 0.562486513730942 1103 76-77 0.6150628928645006 1104 1 0.7424086209084493 1104 2 0.7701311398328983 1104 3 0.7780528516589129 1104 4 0.7445162502034322 1104 5 0.7345310837438035 1104 6 0.7720689684499646 1104 7 0.7005160243219208 1104 8 0.7127950721211604 1104 9 0.6384682399374455 1104 10-11 0.8000389971814457 1104 12-13 0.835921651448615 1104 14-15 0.8838684218903978 1104 16-17 0.9102627794709122 1104 18-19 0.778259190365425 1104 20-21 0.7448361372314238 1104 22-23 0.7974080080456503 1104 24-25 0.8471875448467685 1104 26-27 0.6268806018663966 1104 28-29 0.7026286743372836 1104 30-31 0.8973051460020898 1104 32-33 0.8945589948284223 1104 34-35 0.9016933854421083 1104 36-37 0.8488093641174004 1104 38-39 0.7951071475578573 1104 40-41 0.87923312852881 1104 42-43 0.9404415189189486 1104 44-45 0.8153461149588601 1104 46-47 0.7295926398622115 1104 48-49 0.6990406058588938 1104 50-51 0.7336558507337116 1104 52-53 0.7512877624443668 1104 54-55 0.7110437755621071 1104 56-57 0.7010606853534753 1104 58-59 0.5857962886987629 1104 60-61 0.518615645021157 1104 62-63 0.47024663672819145 1104 64-65 0.4788048797900366 1104 66-67 0.5950273649147988 1104 68-69 0.4466754167338287 1104 70-71 0.41701547549019935 1104 72-73 0.2759002237725703 1104 74-75 0.31202275444843863 1104 76-77 0.3096751239882849 1105 1 0.4596172529178659 1105 2 0.6807236506677938 1105 3 0.5438265159025093 1105 4 0.5252517328022677 1105 5 0.41090142686160647 1105 6 0.5274028163758473 1105 7 0.5520424484888551 1105 8 0.47516056267934914 1105 9 0.4921168373521425 1105 10-11 0.6147234004584874 1105 12-13 0.6867173061073188 1105 14-15 0.6592389966550058 1105 16-17 0.7082569907055358 1105 18-19 0.6752678206114453 1105 20-21 0.6754781194867974 1105 22-23 0.5441961730583245 1105 24-25 0.647503193670218 1105 26-27 0.4529614666383637 1105 28-29 0.5495943817938169 1105 30-31 0.8386108163791164 1105 32-33 0.8562672978031927 1105 34-35 0.7481024191863881 1105 36-37 0.7667611094437845 1105 38-39 0.6865853507161077 1105 40-41 0.789720396371024 1105 42-43 0.8718410423196836 1105 44-45 0.7160719950363621 1105 46-47 0.7291929088610587 1105 48-49 0.5942540134795102 1105 50-51 0.6321228138120993 1105 52-53 0.6264683708017635 1105 54-55 0.5290006507830292 1105 56-57 0.5950901293450421 1105 58-59 0.6116874275273396 1105 60-61 0.6986730998400077 1105 62-63 0.5853930511322929 1105 64-65 0.7142011521201042 1105 66-67 0.6389273191465179 1105 68-69 0.5640474038225243 1105 70-71 0.43712337961567727 1105 72-73 0.029365507612038044 1105 74-75 -0.12061243229976171 1105 76-77 -0.22614925407211928 1106 1 0.02197705360262603 1106 2 0.14220889612743548 1106 3 0.1303821710906803 1106 4 0.14227906631661824 1106 5 0.11818692292614941 1106 6 0.07582499110306884 1106 7 -0.24801452321028705 1106 8 -0.03522772092183146 1106 9 0.18986242674879605 1106 10-11 -0.15748928917973615 1106 12-13 0.16621973111679011 1106 14-15 0.062314352439962306 1106 16-17 0.05225814599681655 1106 18-19 0.09562652049719844 1106 20-21 0.13974798220269236 1106 22-23 0.1226256616208019 1106 24-25 -0.05412915252412631 1106 26-27 -0.15184490806900186 1106 28-29 0.11478031056783067 1106 30-31 0.39149486141833023 1106 32-33 0.10671563886050706 1106 34-35 0.06052589540369269 1106 36-37 0.15474533537307167 1106 38-39 0.28051664337856863 1106 40-41 0.16094440864750936 1106 42-43 0.3314745700660957 1106 44-45 0.16826188453734048 1106 46-47 0.27452257808903724 1106 48-49 0.1353071617593926 1106 50-51 0.09660204099152736 1106 52-53 0.08671647198764632 1106 54-55 0.012730718679993203 1106 56-57 0.014347560465875375 1106 58-59 -0.05442855701693006 1106 60-61 -0.3058950510369627 1106 62-63 -0.2283690262801521 1106 64-65 -0.201507119965747 1106 66-67 -0.3130896868438029 1106 68-69 -0.15825694764707166 1106 70-71 -0.29939949198253046 1106 72-73 -0.4260845920701044 1106 74-75 -0.33184244285150655 1106 76-77 -0.28586809271014957 1107 1 -0.11386007014284871 1107 2 0.005472426105860961 1107 3 -0.03400801133362563 1107 4 -0.13076719650858593 1107 5 -0.03566511067599265 1107 6 -0.388396377108549 1107 7 -0.20516605527319598 1107 8 -0.2375056278689769 1107 9 -0.27536067327034885 1107 10-11 -0.1654199400166192 1107 12-13 -0.1758089345055751 1107 14-15 -0.36595740215902595 1107 16-17 -0.2906212456367214 1107 18-19 -0.2516678529156522 1107 20-21 -0.282450332912191 1107 22-23 -0.32578204092079943 1107 24-25 -0.330389766270919 1107 26-27 -0.5122169695293124 1107 28-29 -0.3176858282753585 1107 30-31 -0.0973512672878627 1107 32-33 -0.04661718578875451 1107 34-35 -0.21602819594406952 1107 36-37 -0.15317887079009296 1107 38-39 -0.17624748125019352 1107 40-41 -0.17054175727091803 1107 42-43 -0.19121578168245534 1107 44-45 -0.1598847503341112 1107 46-47 -0.192034755057108 1107 48-49 -0.1692955208623239 1107 50-51 -0.14765802747457712 1107 52-53 -0.20796328096816907 1107 54-55 -0.26100591387888983 1107 56-57 -0.2802137567905376 1107 58-59 -0.322408055840981 1107 60-61 -0.3238307054997307 1107 62-63 -0.24564445400762125 1107 64-65 -0.2816111706881266 1107 66-67 -0.16509948775863847 1107 68-69 -0.4026459543266725 1107 70-71 -0.4412644548394198 1107 72-73 -0.502871671458955 1107 74-75 -0.5436543956386473 1107 76-77 -0.4314879112741288 1108 1 -0.03278566767478708 1108 2 0.17358292768510353 1108 3 0.1855511827026035 1108 4 0.11238688264132435 1108 5 0.0971968897255664 1108 6 0.1910543533581901 1108 7 0.21131276475369987 1108 8 0.17264875568704952 1108 9 0.17902525755390286 1108 10-11 0.2712646455916641 1108 12-13 0.24153690322653887 1108 14-15 0.27089685207836567 1108 16-17 0.2954499508520314 1108 18-19 0.13622913260022784 1108 20-21 0.18910187921353128 1108 22-23 0.17947850696702972 1108 24-25 0.2642715333488965 1108 26-27 0.14123357095346734 1108 28-29 0.24985131035032282 1108 30-31 0.3430997071355435 1108 32-33 0.33854148320089905 1108 34-35 0.40746692600188084 1108 36-37 0.386636520933088 1108 38-39 0.4703293532284434 1108 40-41 0.4167908247622165 1108 42-43 0.3487049216036233 1108 44-45 0.3123115937700156 1108 46-47 0.24510438876290763 1108 48-49 0.3484319471480468 1108 50-51 0.27868296782548185 1108 52-53 0.4070720063933635 1108 54-55 0.4275470776128536 1108 56-57 0.4034041207000385 1108 58-59 0.4008042058964634 1108 60-61 0.4021722047091032 1108 62-63 0.34983841829077633 1108 64-65 0.4122361987896568 1108 66-67 0.36216349106030776 1108 68-69 0.29309320201501166 1108 70-71 0.3531668346551129 1108 72-73 0.2519615420549499 1108 74-75 0.33208890580760553 1108 76-77 0.3995659383837946 1109 1 -0.02466437008480682 1109 2 -0.07210806552922833 1109 3 -0.12382435320832741 1109 4 0.052815002023777424 1109 5 0.06356231460227946 1109 6 -0.1660343826101922 1109 7 -0.16645636590627788 1109 8 -0.13713718462172153 1109 9 -0.028751054856599012 1109 10-11 -0.21813282730267503 1109 12-13 -0.16619731977334595 1109 14-15 -0.03809648412766364 1109 16-17 -0.031225638595586247 1109 18-19 -0.13834165987329783 1109 20-21 -0.060503951497373976 1109 22-23 -0.026507220936025533 1109 24-25 -0.1761340179266213 1109 26-27 -0.29122635270235264 1109 28-29 -0.07416043725249466 1109 30-31 -0.08121689489536266 1109 32-33 -0.07142506725198672 1109 34-35 -0.03405051259414904 1109 36-37 -0.046333437586859816 1109 38-39 -0.07344243685551533 1109 40-41 0.06922737508452315 1109 42-43 0.025950850856848007 1109 44-45 0.006899774784951518 1109 46-47 -0.09097457131961662 1109 48-49 -0.01269212738546699 1109 50-51 -0.04023970430802137 1109 52-53 -0.055390289416411775 1109 54-55 0.12150250288129527 1109 56-57 0.09126728876605483 1109 58-59 0.0929318103691692 1109 60-61 0.19937517403649707 1109 62-63 0.10758182522616977 1109 64-65 0.25161214819530997 1109 66-67 0.28773570247363267 1109 68-69 0.08711709330507134 1109 70-71 0.15678140969135157 1109 72-73 0.07335887131427299 1109 74-75 0.04646790201651285 1109 76-77 0.006740833595522133 1110 1 -0.6020434128230789 1110 2 -0.6159423867596558 1110 3 -0.7562263568968888 1110 4 -0.746051209126982 1110 5 -0.793458053485864 1110 6 -0.7231176687895697 1110 7 -0.7534625168591731 1110 8 -1.034611497393854 1110 9 -0.9488776108940158 1110 10-11 -0.8044734152511239 1110 12-13 -0.7511128631375144 1110 14-15 -0.6534964569946737 1110 16-17 -0.7531364139589627 1110 18-19 -0.7717071184641568 1110 20-21 -0.9330923076359099 1110 22-23 -0.9189969707898911 1110 24-25 -0.9267714615971059 1110 26-27 -0.9374782963972379 1110 28-29 -0.7531152095966362 1110 30-31 -0.7271120691331419 1110 32-33 -0.6927784222432649 1110 34-35 -0.7845935185630637 1110 36-37 -0.7233877323003242 1110 38-39 -0.7830516957821487 1110 40-41 -0.7631216517788744 1110 42-43 -0.7672486223339199 1110 44-45 -0.7296859618429643 1110 46-47 -0.7699472102559461 1110 48-49 -0.8194707730268327 1110 50-51 -0.7737303242173397 1110 52-53 -0.6861149532043491 1110 54-55 -0.6506730527826967 1110 56-57 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-0.8053194796877357 2314 46-47 -0.6394430969317355 2314 48-49 -0.5542908221262728 2314 50-51 -0.5607709011920505 2314 52-53 -0.37823492690222693 2314 54-55 -0.6607079722284261 2314 56-57 -0.647118555564191 2314 58-59 -0.6204015890365078 2314 60-61 -0.5316500044827208 2314 62-63 -0.5817744979799251 2314 64-65 -0.47584223297935324 2314 66-67 -0.4494876402254562 2314 68-69 -0.43743968513948417 2314 70-71 -0.4132578372821243 2314 72-73 -0.45913860554801644 2314 74-75 -0.3942737931025597 2314 76-77 -0.37074716188512724 2315 1 -1.4801049831311985 2315 2 -1.4128250521278218 2315 3 -1.319485334484959 2315 4 -1.1995139072968115 2315 5 -1.4377984323237314 2315 6 -1.3876600749420618 2315 7 -1.8520617359349671 2315 8 -1.8617172830159632 2315 9 -1.3878800644745581 2315 10-11 -1.5757879745816652 2315 12-13 -1.4720942075419217 2315 14-15 -1.392807438925587 2315 16-17 -1.4142435339816188 2315 18-19 -1.376666195908598 2315 20-21 -1.297771020136551 2315 22-23 -1.5160175537608893 2315 24-25 -1.600984295336005 2315 26-27 -1.4487206913993518 2315 28-29 -1.4951545344834045 2315 30-31 -1.5181597972308865 2315 32-33 -1.474603912507888 2315 34-35 -1.5950349128807417 2315 36-37 -1.5868956756616974 2315 38-39 -1.5116117775228801 2315 40-41 -1.7426006457396106 2315 42-43 -1.854527969494935 2315 44-45 -1.8035814363959908 2315 46-47 -1.5970538864172816 2315 48-49 -1.6010363500987346 2315 50-51 -1.504157100437176 2315 52-53 -1.526634972608992 2315 54-55 -1.6683589132896266 2315 56-57 -1.7659517598694876 2315 58-59 -1.6543070543163658 2315 60-61 -1.7735438343064658 2315 62-63 -1.585551039337826 2315 64-65 -1.4913396934070207 2315 66-67 -1.6336632143079797 2315 68-69 -1.7262380281505791 2315 70-71 -1.7217667884541719 2315 72-73 -1.5519526003589554 2315 74-75 -1.6205246204503858 2315 76-77 -1.6413576912058616 2316 1 -3.0737554945564654 2316 2 -2.6820203712300454 2316 3 -2.799904823200322 2316 4 -4.020582047592974 2316 5 -3.3190678734334362 2316 6 -3.270774246891193 2316 7 -2.921123781099073 2316 8 -2.6884710261287594 2316 9 -2.9524262837922812 2316 10-11 -2.9070019061920576 2316 12-13 -3.579104760054353 2316 14-15 -4.7336221806632715 2316 16-17 -3.8476752155180876 2316 18-19 -3.433417182038937 2316 20-21 -3.1465313779981727 2316 22-23 -3.50509325050853 2316 24-25 -3.471010677073693 2316 26-27 -3.4368201204784157 2316 28-29 -4.0632817717349425 2316 30-31 -4.122907596804563 2316 32-33 -4.1480861886448395 2316 34-35 -4.078747050489142 2316 36-37 -4.047823622623895 2316 38-39 -4.216796635068441 2316 40-41 -3.968445292381034 2316 42-43 -4.183963515007608 2316 44-45 -4.121422382160603 2316 46-47 -4.083065259723298 2316 48-49 -3.8330720387297568 2316 50-51 -3.953395222628025 2316 52-53 -3.861797283900895 2316 54-55 -4.0126560690590445 2316 56-57 -3.7944747478430543 2316 58-59 -3.884957197169882 2316 60-61 -3.6252981755721265 2316 62-63 -3.6883017389027195 2316 64-65 -3.6219418744458878 2316 66-67 -3.650311886162047 2316 68-69 -3.8642032426847273 2316 70-71 -3.652347289876129 2316 72-73 -3.619223223345667 2316 74-75 -3.772817833524858 2316 76-77 -4.072940144341267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 264963.0 3 18251.0 4 22032.0 5 26508.0 6 29664.0 7 31547.0 8 34298.0 9 34104.0 10 37205.0 11 40012.0 12 36496.0 13 34948.0 14 36011.0 15 37896.0 16 38492.0 17 40701.0 18 42541.0 19 45146.0 20 47811.0 21 51419.0 22 56500.0 23 63837.0 24 72711.0 25 84722.0 26 98306.0 27 114162.0 28 133417.0 29 156077.0 30 182666.0 31 216465.0 32 260061.0 33 322928.0 34 419126.0 35 554477.0 36 776056.0 37 1023755.0 38 873293.0 39 201641.0 40 1186.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.064793540840313 26.721686880117662 27.659667211534433 22.55385236750759 2 22.817186840072875 26.755407106114337 27.657438654736723 22.769967399076066 3 23.05601058184445 26.930751442954538 27.200937221208015 22.812300753992997 4 22.590181187046333 26.847197227150097 27.665970242898748 22.896651342904818 5 23.028236575942863 26.82427462061569 27.475216058293135 22.672272745148312 6 23.089705782155054 26.774459507720927 27.427392868308086 22.708441841815937 7 22.966796143202817 26.42613968771369 27.589812723943037 23.017251445140452 8 22.84803423316527 26.835618125578108 27.44747559063856 22.868872050618062 9 22.83759878602465 26.63688128452128 27.5759896894409 22.949530240013168 10-11 23.248182117307138 26.772080730355803 27.234341948301953 22.7453952040351 12-13 23.22683628390924 26.541936419143386 27.262245176467108 22.968982120480263 14-15 22.952870948367597 26.810076590986633 27.41491073733219 22.822141723313585 16-17 23.186829073164084 26.573669531741718 27.471349185984845 22.768152209109353 18-19 23.132704361198304 26.491959410735305 27.322860185673647 23.052476042392744 20-21 23.096310546042783 26.71468258804018 27.24636037654591 22.942646489371132 22-23 23.134829208511263 26.779209159078942 26.94878503647215 23.137176595937646 24-25 23.20749472239416 26.825252843756264 26.90541254425761 23.06183988959196 26-27 23.138513255521755 26.833798512898845 26.913601285502175 23.114086946077226 28-29 23.300935210766127 26.791948755607862 26.87346990660086 23.033646127025147 30-31 23.191304838215547 26.628338533339225 26.98199482082461 23.198361807620614 32-33 23.073937973561996 27.03166679952683 26.954381816926425 22.940013409984754 34-35 23.18627419599207 26.74006743798751 26.97595711673186 23.097701249288566 36-37 23.22054463506122 26.729137403434667 27.199066391282685 22.851251570221432 38-39 23.094029917747573 26.749488423146712 27.180273136196092 22.976208522909626 40-41 22.987269704133436 26.73839221797914 27.100751843254805 23.17358623463262 42-43 22.938901603156133 26.94517618803878 26.88027194542312 23.235650263381967 44-45 22.902741022537267 26.742632832181627 27.178810262779578 23.175815882501517 46-47 22.91767524952899 26.62834051789841 27.167563975147708 23.286420257424897 48-49 23.078406890165056 26.64812855711028 27.067171838535536 23.206292714189132 50-51 22.901943178409823 26.817089237877862 26.97364263917372 23.307324944538603 52-53 23.022164303632415 26.666530475317213 27.01805660991629 23.293248611134086 54-55 22.98277310146142 26.67582696712269 27.053123416260043 23.288276515155847 56-57 22.885350623330705 26.907169713075508 27.007224557275606 23.20025510631818 58-59 23.01036921686046 26.80745630003606 27.002233042644264 23.17994144045922 60-61 22.966189468334512 26.691077258295394 26.91633954537947 23.426393727990625 62-63 22.950994234001502 26.66383474915235 27.10283023808001 23.282340778766137 64-65 23.001499759796058 26.573560676095404 27.039936659024193 23.38500290508434 66-67 22.89606798757862 26.755932552958665 27.118523927730497 23.229475531732213 68-69 22.925304996220394 26.52075062788239 27.255160930664662 23.298783445232548 70-71 22.815530353398145 26.44519913337001 27.19854445443422 23.540726058797624 72-73 22.883023806822273 26.431407607495494 27.164702997295148 23.520865588387082 74-75 22.7873696194029 26.26934336650915 27.16026155889025 23.7830254551977 76-77 22.64710819046652 26.155725192702526 27.48810405970768 23.70906255712328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5127.0 1 3252.5 2 1327.0 3 1377.5 4 1479.0 5 1611.0 6 1816.0 7 2037.0 8 2251.0 9 2317.0 10 2445.5 11 2664.0 12 2940.5 13 3127.0 14 3315.5 15 3762.0 16 4387.0 17 4754.0 18 5258.5 19 6477.0 20 8316.0 21 11218.0 22 12995.0 23 15433.5 24 21551.0 25 30161.0 26 35092.0 27 41579.0 28 56996.0 29 74730.0 30 83534.0 31 96758.0 32 122834.5 33 149457.5 34 175815.5 35 188403.0 36 203337.5 37 230850.5 38 250166.5 39 256904.0 40 264136.5 41 280813.5 42 302201.0 43 314144.0 44 316371.5 45 321271.0 46 327840.5 47 332380.5 48 333023.0 49 334402.0 50 331391.5 51 316695.5 52 306389.0 53 300835.5 54 281856.5 55 251437.0 56 218735.5 57 203028.0 58 190431.0 59 159416.5 60 128643.0 61 116287.0 62 101572.0 63 73951.5 64 52884.0 65 44722.0 66 38658.0 67 28906.5 68 21345.0 69 14865.0 70 12259.0 71 10403.5 72 6996.5 73 4849.5 74 4254.0 75 3614.5 76 2448.5 77 1561.0 78 1200.0 79 989.5 80 633.5 81 400.0 82 276.0 83 240.0 84 208.5 85 177.5 86 158.0 87 138.0 88 123.5 89 102.5 90 89.5 91 83.0 92 76.5 93 71.5 94 68.0 95 62.5 96 62.0 97 65.0 98 70.5 99 145.5 100 218.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0031395590382646715 2 0.008748091689145249 3 0.011582839170296845 4 0.019035481741711525 5 0.014219459139324942 6 0.013472670824397908 7 0.017816235513259226 8 0.018944038274577604 9 0.017770513779692264 10-11 0.021268226397564798 12-13 0.020231867103380343 14-15 0.008252772908836501 16-17 0.00616481374261194 18-19 0.01188765072740992 20-21 0.01052361900932891 22-23 0.014417586651448443 24-25 0.021359669864698724 26-27 0.013442189668686603 28-29 0.008489001865599135 30-31 0.008245152619908675 32-33 0.009875894450463626 34-35 0.01123230587961681 36-37 0.009944477050814068 38-39 0.01642172263946691 40-41 0.012520134708419551 42-43 0.015194856122086783 44-45 0.017404739911156576 46-47 0.01632265888340516 48-49 0.013586975158315313 50-51 0.025733715709271347 52-53 0.030976474491616238 54-55 0.024423026013685126 56-57 0.015682554613467705 58-59 0.01675701535229129 60-61 0.019820371501277696 62-63 0.025642272242137425 64-65 0.021039617729729992 66-67 0.01968320630057681 68-69 0.028690387813268173 70-71 0.05509468894818829 72-73 0.06582405575856852 74-75 0.07694967759319575 76-77 0.07448832426950767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 6561431.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.30389320281504 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37724783878544 34.23725602079582 2 13.732477664792702 12.991993137307938 3 5.406689408093945 7.672723750238017 4 2.6595329287595204 5.03225046525641 5 1.5237085186090518 3.603867251825105 6 0.9598183049864574 2.7241885555911804 7 0.6390590622744869 2.1160987142486416 8 0.4539774126615762 1.7179919236026793 9 0.3302102091782806 1.405820562250374 >10 1.6841770257980104 14.497604988228439 >50 0.14102808442002213 4.6383326611653795 >100 0.08717644788393551 7.467133495340541 >500 0.003956619117470542 1.2263122640204152 >1k 9.080444793252486E-4 0.5754920787741231 >5k 3.243015986216331E-5 0.09293413135502812 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.572173356696123E-5 0.0 2 0.0 0.0 0.0 1.5240577855653745E-4 0.0 3 0.0 0.0 0.0 1.9812751212349867E-4 0.0 4 0.0 0.0 0.0 5.94382536370496E-4 0.0 5 0.0 0.0 0.0 0.0011125621834627232 0.0 6 0.0 0.0 0.0 0.002011756276946294 0.0 7 0.0 1.5240577855653743E-5 0.0 0.0043740458445726245 1.5240577855653743E-5 8 0.0 1.5240577855653743E-5 0.0 0.006964944080033761 1.5240577855653743E-5 9 0.0 1.5240577855653743E-5 0.0 0.009601564049061859 1.5240577855653743E-5 10 0.0 1.5240577855653743E-5 0.0 0.012908769443738722 1.5240577855653743E-5 11 0.0 1.5240577855653743E-5 0.0 0.0162769371498382 1.5240577855653743E-5 12 0.0 1.5240577855653743E-5 0.0 0.021214884375070013 1.5240577855653743E-5 13 0.0 1.5240577855653743E-5 0.0 0.028591324057206425 1.5240577855653743E-5 14 0.0 1.5240577855653743E-5 0.0 0.03788807654915521 1.5240577855653743E-5 15 0.0 1.5240577855653743E-5 0.0 0.04837359411384498 1.5240577855653743E-5 16 0.0 1.5240577855653743E-5 0.0 0.05838665376500949 1.5240577855653743E-5 17 0.0 1.5240577855653743E-5 0.0 0.06934462924322453 1.5240577855653743E-5 18 0.0 1.5240577855653743E-5 0.0 0.08092746841352139 1.5240577855653743E-5 19 0.0 1.5240577855653743E-5 0.0 0.0928151191409313 1.5240577855653743E-5 20 0.0 1.5240577855653743E-5 0.0 0.10590677551893786 1.5240577855653743E-5 21 0.0 1.5240577855653743E-5 0.0 0.11951661154403666 1.5240577855653743E-5 22 0.0 1.5240577855653743E-5 0.0 0.13463526477684518 1.5240577855653743E-5 23 0.0 1.5240577855653743E-5 0.0 0.15062263094742595 1.5240577855653743E-5 24 0.0 1.5240577855653743E-5 0.0 0.16807309259214948 1.5240577855653743E-5 25 0.0 1.5240577855653743E-5 0.0 0.18584360637184175 1.5240577855653743E-5 26 0.0 1.5240577855653743E-5 0.0 0.20475716349070805 1.5240577855653743E-5 27 0.0 1.5240577855653743E-5 0.0 0.22452419296949094 1.5240577855653743E-5 28 0.0 1.5240577855653743E-5 0.0 0.2437425616454703 1.5240577855653743E-5 29 0.0 1.5240577855653743E-5 0.0 0.2638753649927889 3.0481155711307487E-5 30 0.0 1.5240577855653743E-5 0.0 0.28537982034711634 3.0481155711307487E-5 31 0.0 1.5240577855653743E-5 0.0 0.30965806087117276 4.572173356696123E-5 32 0.0 1.5240577855653743E-5 0.0 0.33611570402858765 4.572173356696123E-5 33 0.0 1.5240577855653743E-5 0.0 0.3642802919058358 4.572173356696123E-5 34 0.0 1.5240577855653743E-5 0.0 0.3951729432192459 4.572173356696123E-5 35 0.0 1.5240577855653743E-5 0.0 0.42728484076110834 4.572173356696123E-5 36 0.0 1.5240577855653743E-5 0.0 0.46192667422700934 4.572173356696123E-5 37 0.0 1.5240577855653743E-5 0.0 0.49655326711505465 4.572173356696123E-5 38 0.0 1.5240577855653743E-5 0.0 0.5318504454287487 4.572173356696123E-5 39 0.0 1.5240577855653743E-5 0.0 0.5679096526352254 4.572173356696123E-5 40 0.0 1.5240577855653743E-5 0.0 0.6056758045615355 4.572173356696123E-5 41 0.0 1.5240577855653743E-5 0.0 0.6482274979345207 4.572173356696123E-5 42 0.0 1.5240577855653743E-5 0.0 0.6924099331380609 4.572173356696123E-5 43 1.5240577855653743E-5 1.5240577855653743E-5 0.0 0.7426123965945843 4.572173356696123E-5 44 1.5240577855653743E-5 1.5240577855653743E-5 0.0 0.7962897118021968 4.572173356696123E-5 45 1.5240577855653743E-5 1.5240577855653743E-5 0.0 0.852710331023827 4.572173356696123E-5 46 1.5240577855653743E-5 1.5240577855653743E-5 0.0 0.9131697033772054 4.572173356696123E-5 47 1.5240577855653743E-5 1.5240577855653743E-5 0.0 0.9736900380420064 4.572173356696123E-5 48 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.0376547433021852 6.0962311422614974E-5 49 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.1025948455451258 7.620288927826873E-5 50 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.1719394747883503 7.620288927826873E-5 51 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.2449266021390761 7.620288927826873E-5 52 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.3226230680471989 7.620288927826873E-5 53 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.4070101476339536 7.620288927826873E-5 54 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.497005759871589 7.620288927826873E-5 55 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.5908115165731378 7.620288927826873E-5 56 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.691993712956823 9.144346713392246E-5 57 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.7972603842058235 9.144346713392246E-5 58 1.5240577855653743E-5 1.5240577855653743E-5 0.0 1.908806173531353 9.144346713392246E-5 59 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.018812664493462 9.144346713392246E-5 60 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.1329188708987417 9.144346713392246E-5 61 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.2546148850761365 9.144346713392246E-5 62 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.380745907409527 9.144346713392246E-5 63 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.5164175314805566 9.144346713392246E-5 64 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.660501954527907 9.144346713392246E-5 65 1.5240577855653743E-5 1.5240577855653743E-5 0.0 2.811033142008199 1.066840449895762E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTCG 280 0.0 22.81992 10 CGCGGAA 770 0.0 21.209784 59 CGATCGT 910 0.0 20.67225 1 CGGTTAC 795 0.0 19.198963 4 ATTCGCG 890 0.0 19.145676 56 TCGCGGA 880 0.0 18.96201 58 CGTTCGG 335 0.0 18.013596 11 CTCGCGC 995 0.0 17.83652 36 CCTCGCG 1035 0.0 17.83212 35 CGCGCTT 1000 0.0 17.749502 38 CAATTCG 1040 0.0 16.72613 54 CGCTCCG 1045 0.0 16.643183 25 AATTCGC 1050 0.0 16.56696 55 TCGCGCT 1115 0.0 16.236471 37 CGCCTAG 400 7.2759576E-12 15.0846615 26 ACTCGAG 1265 0.0 14.59003 14 CCGCCTA 415 1.4551915E-11 14.539766 25 AACTCGA 1275 0.0 14.477033 13 ATCGTTC 1305 0.0 13.871482 3 GATCGTT 1325 0.0 13.662102 2 >>END_MODULE