FastQCFastQC Report
Thu 26 May 2016
SRR936070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6561431
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79100.12055297083822111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA19600.043.733418100-101
GGTATCA125200.033.653991
CGGTATC17700.033.46238100-101
CGTACAT34550.031.442842100-101
GTATCAA232400.029.807431
ACGTATC8650.028.558737100-101
CCTGTCG18650.025.84781596-97
TACGCAG21300.025.29158100-101
CGTCCTA10150.024.338234100-101
TCGTACA18200.023.87583100-101
TCAACGC297100.022.9093044
ATCAACG297350.022.8700333
CCGTACA18800.022.797207100-101
CCGGTAT8250.022.728127100-101
GCGTATC7100.022.636662100-101
TATCAAC304300.022.543562
GGGTATC30150.022.408642100-101
CGAGTAC22300.022.288898100-101
CAACGCA309200.022.0310235
CCGTATC12350.021.930647100-101
Produced by FastQC (version 0.11.5)