FastQCFastQC Report
Thu 26 May 2016
SRR936069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595300
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88650.19291449959741475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG2003.6379788E-1123.07773852
ACGCGAA2854.105459E-916.19296554
CGCGGAA2201.2056262E-616.13546859
TCGCGCT3159.094947E-1015.77604437
CGATCGT2703.7089194E-815.7753571
AACGCGA2956.4774213E-915.64490353
ACGATCG2402.9601633E-614.80147969
TAGGGCG4101.0913936E-1114.7164537
TTACGGC5600.014.57891358
CGAACGT4650.014.50283530
GCCGTAA5250.014.19843939
CCCGTAT4202.6375346E-1013.5215872
CGTATTA4801.0913936E-1113.30958754
ACGCTTA3751.0591975E-813.25404145
CTCGCGC3253.5810808E-713.1051136
CCCCGTA4354.6020432E-1013.0554681
ACGTAAT6400.012.75543932
TCGCGGA2801.4673429E-512.67731558
TCGAACG5355.456968E-1212.60526829
CGGTTAC4801.6370905E-1012.5701674
Produced by FastQC (version 0.11.5)