##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936069_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4595300 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.39017278523709 39.0 36.0 41.0 29.0 41.0 2 36.249570430657414 39.0 36.0 41.0 29.0 41.0 3 36.16078427959002 39.0 36.0 41.0 29.0 41.0 4 36.04473157356429 39.0 35.0 41.0 28.0 41.0 5 35.972088002959545 39.0 35.0 41.0 28.0 41.0 6 35.879571083498355 39.0 35.0 40.0 28.0 41.0 7 35.71349770417601 39.0 35.0 40.0 27.0 41.0 8 35.52506887471982 38.0 35.0 40.0 27.0 41.0 9 35.43982547385372 38.0 35.0 40.0 27.0 41.0 10-11 35.65179433334059 39.0 35.0 40.0 27.0 41.0 12-13 35.82732596348443 39.0 35.0 41.0 28.0 41.0 14-15 35.77122995234261 39.0 35.0 41.0 27.0 41.0 16-17 35.61353807150785 39.0 35.0 41.0 27.0 41.0 18-19 35.47295301721324 39.0 35.0 41.0 26.5 41.0 20-21 35.28027256109503 39.0 35.0 41.0 25.5 41.0 22-23 35.04210062455117 39.0 34.5 41.0 24.5 41.0 24-25 34.833950122951705 39.0 34.0 41.0 24.0 41.0 26-27 33.91062759776293 38.0 33.0 39.5 23.0 40.5 28-29 33.95745130894609 38.0 33.0 39.5 23.0 40.5 30-31 34.34342186581942 38.0 34.0 40.0 23.0 41.0 32-33 34.197324875416186 38.0 33.5 40.0 22.0 41.0 34-35 33.945661871912606 38.0 33.0 40.0 21.5 41.0 36-37 33.67104215176376 38.0 33.0 40.0 20.0 41.0 38-39 33.40190031118752 37.0 33.0 40.0 19.5 41.0 40-41 33.03777185385067 37.0 32.0 40.0 18.0 41.0 42-43 32.688340695928446 36.0 32.0 39.5 15.0 41.0 44-45 32.326770831066526 36.0 31.5 39.0 13.0 41.0 46-47 31.94315126324723 35.5 31.0 39.0 9.0 40.0 48-49 31.474633538615542 35.0 31.0 38.0 7.0 40.0 50-51 31.025597023045286 35.0 31.0 37.0 4.0 39.5 52-53 30.559976497725934 35.0 30.0 37.0 2.0 39.0 54-55 30.081082627902422 34.5 30.0 36.0 2.0 39.0 56-57 29.61184329641155 34.0 29.0 36.0 2.0 37.5 58-59 29.163233194785978 34.0 29.0 35.0 2.0 37.0 60-61 28.789777707657827 34.0 29.0 35.0 2.0 36.5 62-63 28.433492699062086 34.0 29.0 35.0 2.0 36.0 64-65 28.048859486867016 34.0 27.5 35.0 2.0 36.0 66-67 27.656222118251257 34.0 27.0 35.0 2.0 35.5 68-69 27.286188714556175 33.5 26.0 35.0 2.0 35.0 70-71 26.855707788392486 33.0 25.5 35.0 2.0 35.0 72-73 26.365495723891804 33.0 25.0 35.0 2.0 35.0 74-75 25.776040302047747 33.0 23.5 35.0 2.0 35.0 76-77 24.07671022566535 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0482958853586837 1101 2 1.0604999941743785 1101 3 1.1588469885357924 1101 4 1.1387795702852443 1101 5 1.1494114174383583 1101 6 1.061629518527667 1101 7 1.0868467656072767 1101 8 1.1881824527050568 1101 9 1.1925274749848072 1101 10-11 1.1285710613353999 1101 12-13 1.1507075500825223 1101 14-15 1.2031323786976458 1101 16-17 1.2925347346559661 1101 18-19 1.3175735388867906 1101 20-21 1.2635529751076007 1101 22-23 1.2504247754481241 1101 24-25 1.283660942959564 1101 26-27 1.248591593651632 1101 28-29 1.3323511998818276 1101 30-31 1.3851623461152869 1101 32-33 1.4524111987166464 1101 34-35 1.284033796004728 1101 36-37 1.3266633523686906 1101 38-39 1.4226619082065994 1101 40-41 1.3479835892401368 1101 42-43 1.3004486817868752 1101 44-45 1.305350932404174 1101 46-47 1.3288797548046603 1101 48-49 1.4608244527021874 1101 50-51 1.438040306387208 1101 52-53 1.4388649311446962 1101 54-55 1.397812568278809 1101 56-57 1.4149499793277478 1101 58-59 1.4219048003714931 1101 60-61 1.3028419030305969 1101 62-63 1.408679464163935 1101 64-65 1.2673313362463894 1101 66-67 1.339644614731828 1101 68-69 1.3292895115927372 1101 70-71 1.2122920538390858 1101 72-73 1.1756342916919778 1101 74-75 1.2330028053512727 1101 76-77 1.2506685891992788 1102 1 0.9977715764931006 1102 2 1.1308050466052677 1102 3 1.0470738712812633 1102 4 1.0618014959287194 1102 5 1.1386869179150025 1102 6 1.0587696519881078 1102 7 1.0267895682764845 1102 8 1.1598221095210732 1102 9 1.0871890574442986 1102 10-11 1.0275224436042336 1102 12-13 1.115674184972896 1102 14-15 1.0185518257901123 1102 16-17 1.0805471274109735 1102 18-19 1.0656669611937488 1102 20-21 1.0411983516567034 1102 22-23 1.0812160051907327 1102 24-25 1.1499621822350647 1102 26-27 1.0449452638136947 1102 28-29 0.9940528204728665 1102 30-31 1.094409247926535 1102 32-33 1.0080594351322816 1102 34-35 1.1116076758903333 1102 36-37 1.057120931015021 1102 38-39 1.1871995631160388 1102 40-41 1.1629263919093447 1102 42-43 1.2414639344084293 1102 44-45 1.2743690448922607 1102 46-47 1.122254397321349 1102 48-49 1.171382126677404 1102 50-51 1.1371585142041738 1102 52-53 1.1632452933944606 1102 54-55 1.1258869248088388 1102 56-57 1.2088012662676881 1102 58-59 1.2370859252523303 1102 60-61 1.1154014835835042 1102 62-63 1.1215011991496375 1102 64-65 1.06821312842942 1102 66-67 1.1620945670673883 1102 68-69 1.031505908160895 1102 70-71 1.143655256889609 1102 72-73 1.2059012125690032 1102 74-75 1.096920822510473 1102 76-77 1.3206161583127205 1103 1 0.4962250792543301 1103 2 0.32691423403543496 1103 3 0.4175278485645251 1103 4 0.4878904446814545 1103 5 0.48002637699116235 1103 6 0.4763972406632959 1103 7 0.5166213028533448 1103 8 0.6135930740005051 1103 9 0.4631294723114934 1103 10-11 0.5608834760068859 1103 12-13 0.6374818402375482 1103 14-15 0.6976979397026639 1103 16-17 0.7340203203790239 1103 18-19 0.7481147900208711 1103 20-21 0.729963785631 1103 22-23 0.7240339276912806 1103 24-25 0.6343689168733633 1103 26-27 0.46478396541953515 1103 28-29 0.5276736782630351 1103 30-31 0.7093294806389281 1103 32-33 0.60477865369905 1103 34-35 0.8525058842122135 1103 36-37 0.704702477201721 1103 38-39 0.6994378024427022 1103 40-41 0.7769243617861221 1103 42-43 0.7966053661021562 1103 44-45 0.8075609841391369 1103 46-47 0.594232463858539 1103 48-49 0.6776977769904171 1103 50-51 0.6123568628496372 1103 52-53 0.5891688352501419 1103 54-55 0.7122809606209515 1103 56-57 0.5871335045215353 1103 58-59 0.6454873668699221 1103 60-61 0.7226922498628596 1103 62-63 0.5429732205576414 1103 64-65 0.6085515504381256 1103 66-67 0.7099816929316738 1103 68-69 0.5429065568977514 1103 70-71 0.550726657263553 1103 72-73 0.46795575333601036 1103 74-75 0.48785378219866615 1103 76-77 0.6587879203198135 1104 1 0.7016911320755312 1104 2 0.6293053062697638 1104 3 0.6018842005927638 1104 4 0.6151149750061577 1104 5 0.7039380721770527 1104 6 0.38993148166242264 1104 7 0.6483581285969038 1104 8 0.6723304685837164 1104 9 0.6055556521400263 1104 10-11 0.5148432350829637 1104 12-13 0.7306754343859794 1104 14-15 0.6553758977563078 1104 16-17 0.7126273201673996 1104 18-19 0.6426585967955347 1104 20-21 0.5607107604184165 1104 22-23 0.5337306379966762 1104 24-25 0.5291407131387018 1104 26-27 0.3595426993599844 1104 28-29 0.3361714693637836 1104 30-31 0.5553058418752883 1104 32-33 0.5916976371156935 1104 34-35 0.6407291161746187 1104 36-37 0.557340022936252 1104 38-39 0.5691212449238705 1104 40-41 0.5676457497199081 1104 42-43 0.6825147575093169 1104 44-45 0.6258427298516338 1104 46-47 0.5832496993502829 1104 48-49 0.7036809000294291 1104 50-51 0.6424854158020068 1104 52-53 0.6053692333941747 1104 54-55 0.6423911825952153 1104 56-57 0.5834551210405365 1104 58-59 0.5803192640398009 1104 60-61 0.45600149532176104 1104 62-63 0.4398043005397305 1104 64-65 0.4002252037178984 1104 66-67 0.3531280495595972 1104 68-69 0.35443128218448194 1104 70-71 0.26938634139795425 1104 72-73 0.20255147383417338 1104 74-75 0.14157444607520375 1104 76-77 0.17374398171951455 1105 1 0.2445642577745062 1105 2 0.3992482315600583 1105 3 0.38247038564805536 1105 4 0.378079321865755 1105 5 0.41631333451016417 1105 6 0.09207920454201002 1105 7 0.1897038542931213 1105 8 0.44610440647980454 1105 9 0.27995733417849067 1105 10-11 0.378744236263465 1105 12-13 0.4779294582652014 1105 14-15 0.12978590714507732 1105 16-17 0.22194715686503486 1105 18-19 0.418022713423305 1105 20-21 0.43607643033433163 1105 22-23 0.4282301210969095 1105 24-25 0.5582934505501598 1105 26-27 0.3182359137344619 1105 28-29 0.5034709571238665 1105 30-31 0.47929730483108557 1105 32-33 0.4982557059298074 1105 34-35 0.555645277447347 1105 36-37 0.5631697074806894 1105 38-39 0.5901755263037458 1105 40-41 0.6129773853668326 1105 42-43 0.34232394469088945 1105 44-45 0.20778730853086103 1105 46-47 0.1875062913325607 1105 48-49 0.16857583303093193 1105 50-51 0.3182552858090446 1105 52-53 0.1151163321971076 1105 54-55 0.035739398730079586 1105 56-57 0.22620165375623813 1105 58-59 0.25312990440872696 1105 60-61 0.07901479664585409 1105 62-63 -0.047894304607520866 1105 64-65 0.15271488729584348 1105 66-67 0.214042746426788 1105 68-69 -0.10353382837511305 1105 70-71 -0.39604413083615597 1105 72-73 -0.5442359164001296 1105 74-75 -0.5748385024798957 1105 76-77 -0.2163937819942916 1106 1 0.04497860819216726 1106 2 0.22403833177304477 1106 3 -0.2633361667264893 1106 4 0.01393344065564861 1106 5 -0.1524610257932011 1106 6 -0.0056951797891287015 1106 7 0.181023391106244 1106 8 0.1421508933614959 1106 9 0.06437025837600885 1106 10-11 -0.14776376412784487 1106 12-13 -0.05300941421425165 1106 14-15 0.07963360966061117 1106 16-17 0.17622487333385095 1106 18-19 0.15980832473284323 1106 20-21 0.07672516312658217 1106 22-23 0.20199219719973627 1106 24-25 0.24463335948489373 1106 26-27 0.2879810820061266 1106 28-29 0.37774583316628707 1106 30-31 0.42030879268246935 1106 32-33 0.31134932071993404 1106 34-35 0.2913316952320102 1106 36-37 0.4280044564505019 1106 38-39 0.30598560990324586 1106 40-41 0.13896052902375544 1106 42-43 0.1815507694717695 1106 44-45 0.040395660929085864 1106 46-47 0.28590979578370224 1106 48-49 0.2052201224008705 1106 50-51 0.14453843341339123 1106 52-53 0.12344227841951394 1106 54-55 0.27327981688203806 1106 56-57 0.13366167754373137 1106 58-59 0.03242705763213749 1106 60-61 -0.011506310039187895 1106 62-63 -0.16607213246223296 1106 64-65 -0.13223994906642034 1106 66-67 -0.17248339922615585 1106 68-69 -0.14576060681670455 1106 70-71 -0.2568306285990012 1106 72-73 -0.175789385067052 1106 74-75 -0.2784193061746798 1106 76-77 -0.2775865208003516 1107 1 -0.024479573758448225 1107 2 0.04199692984938963 1107 3 0.024342248991189308 1107 4 0.13228649943842896 1107 5 0.07900668752658646 1107 6 -0.1678490411917437 1107 7 -0.16302878373525687 1107 8 -0.07267096302690845 1107 9 -0.1709456350610452 1107 10-11 -0.0338961620508158 1107 12-13 0.07890236485464897 1107 14-15 0.021688955210507288 1107 16-17 0.06787554126633921 1107 18-19 0.08310655983001425 1107 20-21 -0.05189472722811672 1107 22-23 0.04230050787825945 1107 24-25 -0.16886344373419604 1107 26-27 -0.13055294284222896 1107 28-29 -0.10169205153274419 1107 30-31 0.15563004029818472 1107 32-33 0.20904299403619575 1107 34-35 0.21461632502496286 1107 36-37 0.2359126145426913 1107 38-39 0.23737755254203563 1107 40-41 0.3124763972573703 1107 42-43 0.29090516105705433 1107 44-45 0.261212335093326 1107 46-47 0.2189169932482926 1107 48-49 0.3926981107774914 1107 50-51 0.3073560319106221 1107 52-53 0.2659205678716887 1107 54-55 0.14579198612829458 1107 56-57 0.1806248081004398 1107 58-59 0.24270417336558125 1107 60-61 0.11737257254217326 1107 62-63 0.2704688487833451 1107 64-65 0.26583325016034465 1107 66-67 0.3318845500706509 1107 68-69 0.2519947818149326 1107 70-71 0.24597589218018 1107 72-73 0.2934120601369905 1107 74-75 0.21986050227091525 1107 76-77 0.33500839042271124 1108 1 0.09736833010179424 1108 2 0.052868943004128255 1108 3 0.03245547332744536 1108 4 0.05777799188896182 1108 5 -0.02825286861080656 1108 6 0.08611179933635071 1108 7 0.007189637038301555 1108 8 0.11181542087145857 1108 9 0.08992963264732623 1108 10-11 -0.1724137433445705 1108 12-13 -0.09399198160782163 1108 14-15 0.12910163383003948 1108 16-17 0.10514890792533294 1108 18-19 -0.0058473876266234015 1108 20-21 -0.0031004601182473834 1108 22-23 0.027101157326264058 1108 24-25 0.033267594102731834 1108 26-27 -0.15106886882368542 1108 28-29 0.019578613968626257 1108 30-31 0.05217480138103525 1108 32-33 0.03148017932269198 1108 34-35 0.06912909884324137 1108 36-37 0.09350619514896152 1108 38-39 -0.039111235094068775 1108 40-41 0.02344426737752059 1108 42-43 0.050834633539004415 1108 44-45 -0.028114533014367282 1108 46-47 -0.0988196414457363 1108 48-49 -0.04716285761676886 1108 50-51 -0.0877950976145172 1108 52-53 -0.11924198306451572 1108 54-55 0.016270454269630363 1108 56-57 -0.10263028186297873 1108 58-59 -0.18882201725862302 1108 60-61 -0.14201288972024173 1108 62-63 -0.047743462693283334 1108 64-65 -0.19373249243249901 1108 66-67 -0.1851578773631637 1108 68-69 -0.21370597721523765 1108 70-71 -0.3807516724263671 1108 72-73 -0.3331266672228388 1108 74-75 -0.4322347354760545 1108 76-77 -0.2914641732025949 1109 1 -0.029601597159093274 1109 2 0.025555223900155966 1109 3 -0.16035746772129755 1109 4 0.04902468967783591 1109 5 -0.10961862694323798 1109 6 -0.007658692466932848 1109 7 -0.14508988382443277 1109 8 0.07346634549057285 1109 9 -0.05584172768271145 1109 10-11 -0.13296815292731168 1109 12-13 -0.08505556500448819 1109 14-15 0.07568144782055697 1109 16-17 0.14552744083887603 1109 18-19 0.052066338715071936 1109 20-21 0.06693862358602587 1109 22-23 0.02195368871988279 1109 24-25 0.05048396583218562 1109 26-27 -0.005669165570331813 1109 28-29 0.14267454998715579 1109 30-31 0.1418404185778641 1109 32-33 0.12518191275734836 1109 34-35 0.13867239852115887 1109 36-37 0.2564461582365709 1109 38-39 0.23116054140503906 1109 40-41 0.30081588826558203 1109 42-43 0.277668129721107 1109 44-45 0.20939351895248848 1109 46-47 0.054447466722038484 1109 48-49 0.23047619713660694 1109 50-51 0.3671113548155489 1109 52-53 0.4416486187521542 1109 54-55 0.47778313837920905 1109 56-57 0.521615004569906 1109 58-59 0.4983682986679874 1109 60-61 0.399698469886026 1109 62-63 0.3367645530849508 1109 64-65 0.37617703602984776 1109 66-67 0.3729896274318243 1109 68-69 0.46377420951801795 1109 70-71 0.5439217253227007 1109 72-73 0.5849263852868454 1109 74-75 0.6047996075196345 1109 76-77 0.588091068718299 1110 1 -0.3155596029574568 1110 2 -0.4053130383620953 1110 3 -0.41262528569343004 1110 4 -0.3396552554901291 1110 5 -0.1728301221482198 1110 6 -0.5089010592809942 1110 7 -0.6011500651940906 1110 8 -0.370379622778799 1110 9 -0.3234350446445262 1110 10-11 -0.42193201984108697 1110 12-13 -0.2978377610471554 1110 14-15 -0.3929364138693927 1110 16-17 -0.4484280660018811 1110 18-19 -0.3019413366547141 1110 20-21 -0.46593570757327285 1110 22-23 -0.5462624153326914 1110 24-25 -0.5272424010990164 1110 26-27 -0.3584152299212775 1110 28-29 -0.2762131489219257 1110 30-31 -0.3260064895896235 1110 32-33 -0.22049839079905809 1110 34-35 -0.047454133519742925 1110 36-37 -0.05883248529800511 1110 38-39 -0.04928469697905058 1110 40-41 -0.027038215561084655 1110 42-43 -0.06326282960250751 1110 44-45 -0.09243495940265944 1110 46-47 -0.09413655026864731 1110 48-49 -0.031866798739837776 1110 50-51 -0.05955225787949203 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-1.0801319736413362 2314 18-19 -0.8078579178534468 2314 20-21 -0.6057745215052961 2314 22-23 -0.4975064059502543 2314 24-25 -0.43541008610581855 2314 26-27 -0.4982358269897489 2314 28-29 -0.5482720537613304 2314 30-31 -0.6791943999710384 2314 32-33 -0.8154045832638914 2314 34-35 -0.7290755065451222 2314 36-37 -0.8118254344987932 2314 38-39 -0.6821067009946802 2314 40-41 -0.5147921763902801 2314 42-43 -0.45674265911128487 2314 44-45 -0.49240860606139236 2314 46-47 -0.6083055452599986 2314 48-49 -0.5021864645366314 2314 50-51 -0.4297488987364133 2314 52-53 -0.43743468681957154 2314 54-55 -0.4708101717441977 2314 56-57 -0.29417927641662445 2314 58-59 -0.4497099878489017 2314 60-61 -0.31982135800420153 2314 62-63 -0.22551205748495917 2314 64-65 -0.24981020949382327 2314 66-67 -0.3016363230219383 2314 68-69 -0.16226331197434973 2314 70-71 -0.1627714460536751 2314 72-73 -0.08907056642748046 2314 74-75 -0.03704921801177008 2314 76-77 -0.09121738045110916 2315 1 -1.5661036034553675 2315 2 -1.5102740148664893 2315 3 -1.422049546370097 2315 4 -1.3198667881585138 2315 5 -1.2668030389402745 2315 6 -1.230189325125096 2315 7 -1.4511188745158137 2315 8 -1.3662747984046675 2315 9 -1.392892712283171 2315 10-11 -1.2990671280022141 2315 12-13 -1.3788208937538897 2315 14-15 -1.7029103855682521 2315 16-17 -1.5597526043379801 2315 18-19 -1.4626379582200926 2315 20-21 -1.4708245837721066 2315 22-23 -1.512657737315429 2315 24-25 -1.5413512860271368 2315 26-27 -1.5071500407560094 2315 28-29 -1.437330674828985 2315 30-31 -1.5863232862556274 2315 32-33 -1.5913001879002664 2315 34-35 -1.5240632771196019 2315 36-37 -1.7575476133851424 2315 38-39 -1.6767297175835445 2315 40-41 -1.6723381618363433 2315 42-43 -1.7928354233194739 2315 44-45 -1.6948582476315401 2315 46-47 -1.620827643446237 2315 48-49 -1.512473447559337 2315 50-51 -1.5219296968380966 2315 52-53 -1.5445281406694882 2315 54-55 -1.5280340029922286 2315 56-57 -1.6581344857828135 2315 58-59 -1.486491865048439 2315 60-61 -1.5025739111231324 2315 62-63 -1.605385926587946 2315 64-65 -1.647749972212278 2315 66-67 -1.6407280403957252 2315 68-69 -1.6475366479976863 2315 70-71 -1.6862249669795126 2315 72-73 -2.930822792073723 2315 74-75 -3.5172436516395216 2315 76-77 -3.187980515402895 2316 1 -3.5187042562832502 2316 2 -3.2794562512697567 2316 3 -2.7673297216686663 2316 4 -2.8729276844847007 2316 5 -3.566744643932232 2316 6 -3.041183882547223 2316 7 -3.3877517750133777 2316 8 -3.2517807224235113 2316 9 -2.821968164902451 2316 10-11 -2.716144617370283 2316 12-13 -2.8709142592397967 2316 14-15 -3.0631684846272265 2316 16-17 -4.244945179928052 2316 18-19 -3.963908947199478 2316 20-21 -3.276425918821282 2316 22-23 -3.519357111210283 2316 24-25 -3.5812583729566185 2316 26-27 -3.253667869584355 2316 28-29 -3.530086003663328 2316 30-31 -3.743033462787185 2316 32-33 -3.806812470362267 2316 34-35 -3.8146062483452496 2316 36-37 -3.653556462911528 2316 38-39 -3.721064446277442 2316 40-41 -3.689870777243442 2316 42-43 -3.9291765726656465 2316 44-45 -3.6944554276236197 2316 46-47 -3.4074261929983543 2316 48-49 -3.5224856766878183 2316 50-51 -3.9239884826920957 2316 52-53 -4.940679219581828 2316 54-55 -3.996674832193346 2316 56-57 -3.9846119162681255 2316 58-59 -4.020538861395693 2316 60-61 -3.8860688135739636 2316 62-63 -3.8945530608891055 2316 64-65 -3.751176103206763 2316 66-67 -3.908014178174046 2316 68-69 -3.955643439138022 2316 70-71 -3.662368371302133 2316 72-73 -3.8549392067561605 2316 74-75 -3.7972069354683704 2316 76-77 -3.7930520294975807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124312.0 3 10208.0 4 13093.0 5 15527.0 6 17492.0 7 19290.0 8 21477.0 9 22327.0 10 26339.0 11 28700.0 12 23815.0 13 21051.0 14 21550.0 15 22261.0 16 22961.0 17 24427.0 18 25920.0 19 27147.0 20 29161.0 21 31596.0 22 35270.0 23 39797.0 24 46070.0 25 54098.0 26 63607.0 27 74452.0 28 87620.0 29 103938.0 30 122482.0 31 146584.0 32 178048.0 33 222651.0 34 291039.0 35 387071.0 36 542094.0 37 744103.0 38 710872.0 39 195548.0 40 1302.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.114132798010296 27.316252210623265 28.996412946820122 21.573202044546317 2 21.81561201812465 27.407164934172023 28.968379805368315 21.808843242335012 3 21.90406046372031 27.700205218143033 28.569133963765907 21.82660035437075 4 21.776093759454103 27.467991536088498 28.813994618860626 21.941920085596767 5 21.912591045553135 27.7016583098968 28.790505014714853 21.595245629835212 6 22.162570378318147 27.452930589077678 28.771396423355917 21.613102609248262 7 22.099617785541582 27.343754250968704 28.74522177514576 21.81140618834395 8 21.957766101399052 27.53615712485099 28.492330221107547 22.01374655264241 9 21.80211710344032 27.45239129086285 28.598918050662913 22.146573555033918 10-11 22.275859066793632 27.50260916882057 28.507805740715547 21.713726023670247 12-13 22.279432665482364 27.29379972480367 28.36670411693189 22.06006349278208 14-15 22.002882327311728 27.51527890281786 28.50550763041965 21.976331139450757 16-17 22.290482787227656 27.29726791737364 28.572846269331855 21.839403026066847 18-19 22.17991746545825 27.333559693890873 28.27332602014609 22.213196820504784 20-21 22.170680035444793 27.470224873301845 28.24169978279073 22.11739530846263 22-23 22.276858615951788 27.44016651022324 27.947641785359618 22.335333088465358 24-25 22.181240954292917 27.586611063897585 27.951502875635654 22.280645106173843 26-27 22.293210812714516 27.62094661152281 27.796593309492224 22.289249266270446 28-29 22.448978481460323 27.53470986697135 27.77021137680568 22.246100274762643 30-31 22.373374942014195 27.39947521961699 27.84697384009074 22.38017599827808 32-33 22.218100389703608 27.653296984791854 27.73474821612554 22.393854409379003 34-35 22.23476085484359 27.466802156446512 27.934907883309172 22.363529105400726 36-37 22.27805664155061 27.633031839801912 27.944725448347256 22.144186070300222 38-39 22.20698574185378 27.511058358379938 28.06934398989771 22.21261190986857 40-41 22.16014504447869 27.432339038095506 27.978813111590817 22.42870280583499 42-43 22.096742125578302 27.547125953202862 27.808347038806957 22.54778488241188 44-45 22.250554860600626 27.39934564809187 27.97568244181105 22.37441704949645 46-47 22.16008594476388 27.209123152921666 28.086114981704302 22.544675920610146 48-49 22.159417037479304 27.31008672042008 27.856984116239015 22.673512125861606 50-51 22.050963827803443 27.502524702244052 27.779812004107445 22.66669946584506 52-53 22.095709942770135 27.32951510653057 27.938800827736017 22.635974122963272 54-55 22.04887727459237 27.305698466789508 27.925273538813506 22.720150719804614 56-57 22.007260547704792 27.49220960883655 27.788338611815576 22.712191231643075 58-59 22.164404211737835 27.361092943225728 27.811991442799023 22.662511402237417 60-61 22.03460348903659 27.276516548583896 27.822616497749227 22.86626346463029 62-63 21.972649160745142 27.29233961657541 27.90856120166855 22.826450021010896 64-65 22.071353403843105 27.07855787757151 27.939709890280202 22.910378828305188 66-67 21.937885478473284 27.144987577182768 28.037484387128682 22.87964255721527 68-69 22.049080539946026 27.04970317946735 28.050432930402835 22.850783350183786 70-71 21.754737203657708 26.960672405036835 28.22809994191955 23.05649044938591 72-73 21.805103436078983 26.797537014264687 28.20746370665806 23.189895842998272 74-75 21.70064289755294 26.69624057120797 28.20345336229376 23.39966316894533 76-77 21.635387677160665 26.59501500838252 28.40956885940867 23.360028455048145 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9553.0 1 5800.0 2 1893.5 3 1762.0 4 1784.0 5 1834.5 6 1951.0 7 2170.0 8 2354.0 9 2385.0 10 2486.5 11 2726.5 12 3027.0 13 3189.0 14 3431.0 15 3898.5 16 4504.0 17 4884.0 18 5349.0 19 6447.0 20 7874.5 21 9940.5 22 11212.0 23 13093.0 24 17765.5 25 24859.5 26 29162.0 27 35361.0 28 50275.5 29 66768.0 30 74545.0 31 86636.0 32 109090.0 33 131697.0 34 154273.5 35 164606.0 36 176406.5 37 196512.5 38 208270.0 39 211722.0 40 214352.0 41 219755.0 42 225778.0 43 229028.0 44 225381.0 45 222403.5 46 222632.5 47 221067.0 48 219942.0 49 215618.5 50 206388.5 51 192473.0 52 183464.0 53 177739.5 54 162741.0 55 143045.0 56 124174.0 57 115725.0 58 108788.5 59 91290.5 60 73422.0 61 66115.0 62 58007.0 63 42141.5 64 29764.5 65 25145.0 66 21217.0 67 14983.0 68 11013.5 69 7922.5 70 6495.0 71 5345.0 72 3650.0 73 2895.0 74 2685.0 75 2417.0 76 1688.0 77 991.5 78 756.0 79 639.5 80 478.5 81 350.5 82 234.0 83 201.0 84 178.0 85 128.5 86 109.0 87 116.0 88 105.5 89 81.0 90 74.5 91 82.0 92 68.5 93 53.0 94 50.0 95 56.0 96 63.0 97 59.0 98 60.0 99 144.0 100 223.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009618523273779732 2 0.014645398559397645 3 0.014732444018888865 4 0.01597284181663874 5 0.0135355689508846 6 0.010663068787674363 7 0.01651687593845886 8 0.018257785128283247 9 0.01773551237133593 10-11 0.02012926250734446 12-13 0.016582160033077276 14-15 0.00874806867886754 16-17 0.007083324266097969 18-19 0.018714773790612146 20-21 0.024220399103431767 22-23 0.021489347811894762 24-25 0.02011838182490806 26-27 0.02468826844819707 28-29 0.00924858007094205 30-31 0.009259460753378452 32-33 0.011446477923095336 34-35 0.013307074619720149 36-37 0.012262529105825518 38-39 0.01520031336365417 40-41 0.012436620024807957 42-43 0.015766108850347092 44-45 0.014743324701325266 46-47 0.025819859421582922 48-49 0.02417687637368616 50-51 0.036754945270167344 52-53 0.03931190564272191 54-55 0.029269035753922486 56-57 0.02216395012295171 58-59 0.017561421452353492 60-61 0.0216416773660044 62-63 0.027299632232933652 64-65 0.027843666354753768 66-67 0.029029660740321633 68-69 0.04247818423171501 70-71 0.055110656540378215 72-73 0.0862185276260527 74-75 0.08655582878158118 76-77 0.09137597110090745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 4595300.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.8063916459451 #Duplication Level Percentage of deduplicated Percentage of total 1 68.68453911389598 32.83559976900146 2 14.66627722039611 14.022835855725187 3 6.271549842990979 8.994605040632765 4 3.3451893491124105 6.396857286140483 5 2.0180266225755137 4.823728553539442 6 1.2462283873444036 3.5746609419408673 7 0.8465293066866988 2.8328658102664286 8 0.5944060474492746 2.27331266408626 9 0.41730264299830394 1.7954760227458435 >10 1.7682558804991901 13.960076662467447 >50 0.08713325930823067 2.877989730900167 >100 0.05177364447858881 4.348344975852747 >500 0.0021943731771751347 0.704590415172531 >1k 5.485930036619297E-4 0.3653098349727373 >5k 4.5716083638394944E-5 0.19374643655566678 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8865 0.19291449959741475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.1761364872804822E-5 0.0 2 0.0 0.0 0.0 2.1761364872804821E-4 0.0 3 0.0 0.0 0.0 4.569886623289013E-4 0.0 4 0.0 0.0 0.0 0.0010880682436402412 0.0 5 0.0 0.0 0.0 0.0022196592170260917 0.0 6 0.0 0.0 0.0 0.004134659325832916 0.0 7 0.0 0.0 0.0 0.008965682327595587 0.0 8 0.0 0.0 0.0 0.014101364437577524 0.0 9 0.0 0.0 0.0 0.019824603399125194 0.0 10 0.0 0.0 0.0 0.027092899266642004 0.0 11 0.0 0.0 0.0 0.03621091114834722 0.0 12 0.0 2.1761364872804822E-5 0.0 0.048593127760973166 0.0 13 0.0 2.1761364872804822E-5 0.0 0.06471829913172154 0.0 14 0.0 2.1761364872804822E-5 0.0 0.08404239113877222 0.0 15 0.0 2.1761364872804822E-5 0.0 0.10547733553848497 0.0 16 0.0 2.1761364872804822E-5 0.0 0.1273039845059082 0.0 17 0.0 2.1761364872804822E-5 0.0 0.1484560311622745 0.0 18 0.0 2.1761364872804822E-5 0.0 0.1701303505755881 0.0 19 0.0 2.1761364872804822E-5 0.0 0.1945030792331295 0.0 20 0.0 2.1761364872804822E-5 0.0 0.22146541031053468 0.0 21 0.0 2.1761364872804822E-5 0.0 0.25095205971318524 0.0 22 0.0 2.1761364872804822E-5 0.0 0.2833547320087916 0.0 23 0.0 2.1761364872804822E-5 0.0 0.3163449611559637 0.0 24 0.0 2.1761364872804822E-5 0.0 0.347528997018693 0.0 25 0.0 2.1761364872804822E-5 0.0 0.3834787717885666 0.0 26 0.0 2.1761364872804822E-5 0.0 0.4187321828825104 0.0 27 0.0 2.1761364872804822E-5 0.0 0.4560746850042435 0.0 28 0.0 2.1761364872804822E-5 0.0 0.49428764172088874 0.0 29 0.0 2.1761364872804822E-5 0.0 0.5325876438970252 0.0 30 0.0 2.1761364872804822E-5 0.0 0.5745435553717929 0.0 31 0.0 2.1761364872804822E-5 0.0 0.6206776489021392 0.0 32 0.0 2.1761364872804822E-5 0.0 0.6729919700563619 0.0 33 0.0 2.1761364872804822E-5 0.0 0.7254150980349487 0.0 34 0.0 2.1761364872804822E-5 0.0 0.7817552716906404 0.0 35 0.0 2.1761364872804822E-5 0.0 0.8381607294409505 0.0 36 0.0 2.1761364872804822E-5 0.0 0.8987878919765848 0.0 37 0.0 2.1761364872804822E-5 0.0 0.9589145431201445 0.0 38 0.0 2.1761364872804822E-5 0.0 1.0224359671838619 0.0 39 0.0 2.1761364872804822E-5 0.0 1.0860444367070703 0.0 40 0.0 2.1761364872804822E-5 0.0 1.1580745544360542 0.0 41 0.0 2.1761364872804822E-5 0.0 1.234935695166801 0.0 42 0.0 2.1761364872804822E-5 0.0 1.3170413248318935 0.0 43 0.0 2.1761364872804822E-5 0.0 1.4088742845951299 0.0 44 0.0 2.1761364872804822E-5 0.0 1.50329684677823 0.0 45 0.0 2.1761364872804822E-5 0.0 1.6035514547472418 0.0 46 0.0 2.1761364872804822E-5 0.0 1.7091158357452179 0.0 47 0.0 2.1761364872804822E-5 0.0 1.8170522055143299 0.0 48 0.0 2.1761364872804822E-5 0.0 1.9274040867843232 0.0 49 0.0 2.1761364872804822E-5 0.0 2.0422823319478596 0.0 50 0.0 2.1761364872804822E-5 0.0 2.159227906774313 0.0 51 0.0 2.1761364872804822E-5 0.0 2.290340130132962 0.0 52 0.0 2.1761364872804822E-5 0.0 2.4278501947642157 0.0 53 0.0 2.1761364872804822E-5 0.0 2.575414009966705 0.0 54 0.0 2.1761364872804822E-5 0.0 2.7364698713903337 0.0 55 0.0 2.1761364872804822E-5 0.0 2.906099710573847 0.0 56 0.0 2.1761364872804822E-5 0.0 3.074140970121646 0.0 57 0.0 2.1761364872804822E-5 0.0 3.2479054686309925 0.0 58 0.0 2.1761364872804822E-5 0.0 3.4309838313058996 0.0 59 0.0 2.1761364872804822E-5 0.0 3.6202641829695557 0.0 60 0.0 2.1761364872804822E-5 0.0 3.8150936826757773 0.0 61 0.0 2.1761364872804822E-5 0.0 4.023415228603138 0.0 62 0.0 2.1761364872804822E-5 0.0 4.24222575239919 0.0 63 0.0 2.1761364872804822E-5 0.0 4.471024742671861 0.0 64 0.0 2.1761364872804822E-5 0.0 4.712118904097665 0.0 65 0.0 2.1761364872804822E-5 0.0 4.964681304811438 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 200 3.6379788E-11 23.077738 52 ACGCGAA 285 4.105459E-9 16.192965 54 CGCGGAA 220 1.2056262E-6 16.135468 59 TCGCGCT 315 9.094947E-10 15.776044 37 CGATCGT 270 3.7089194E-8 15.775357 1 AACGCGA 295 6.4774213E-9 15.644903 53 ACGATCG 240 2.9601633E-6 14.801479 69 TAGGGCG 410 1.0913936E-11 14.716453 7 TTACGGC 560 0.0 14.578913 58 CGAACGT 465 0.0 14.502835 30 GCCGTAA 525 0.0 14.198439 39 CCCGTAT 420 2.6375346E-10 13.521587 2 CGTATTA 480 1.0913936E-11 13.3095875 4 ACGCTTA 375 1.0591975E-8 13.254041 45 CTCGCGC 325 3.5810808E-7 13.10511 36 CCCCGTA 435 4.6020432E-10 13.055468 1 ACGTAAT 640 0.0 12.755439 32 TCGCGGA 280 1.4673429E-5 12.677315 58 TCGAACG 535 5.456968E-12 12.605268 29 CGGTTAC 480 1.6370905E-10 12.570167 4 >>END_MODULE