FastQCFastQC Report
Thu 26 May 2016
SRR936069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595300
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99120.2156986486192414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72910.15866211128761995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52890.11509585881226471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA17250.047.27288100-101
GGTATCA119600.037.1157531
CGGTATC16450.033.831894100-101
CCTGTCG13950.033.48977396-97
CGAGTAC15050.032.23307100-101
CGTACAT25600.032.086308100-101
TACGCAG13550.031.62815100-101
GTATCAA231050.030.8017791
CCGTATC8350.030.295843100-101
ACGTATC7500.029.364397100-101
AGGTATC32350.028.611242100-101
GCGTATC5000.028.570763100-101
TCGAGTA7700.026.282629100-101
TCGTATC8950.026.269674100-101
CCGTACA12450.025.816954100-101
GCGTACA8300.025.458385100-101
CCGGTAT7300.025.276646100-101
TACGGTA3600.024.80087598-99
ATCAACG293500.023.8387583
TCGTACA14150.023.766903100-101
Produced by FastQC (version 0.11.5)