FastQCFastQC Report
Thu 26 May 2016
SRR936068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622339
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91120.19712963501811528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG1951.5526894E-820.02764952
AACGCGA2253.8889993E-918.93420853
TCGAACT13300.016.81511124
TCGCGCT3008.105417E-915.3818837
CGATTGC4500.014.99607368
CTCGAAC14100.014.60193123
GCCTCGA14850.014.58174421
TTAGTCG5150.014.47451815
CGAACTC13800.013.88954125
TAGTCGG5500.013.55253216
ACGCGAA2901.4233756E-613.46497454
CGATCGT2901.4252837E-613.4633711
CTCGCGC3304.3088403E-712.90703336
TTACGGC5550.012.79296858
CGCGGAA3651.0626536E-712.64455759
TTGCGCG3952.1971573E-812.5804095
CGGTTAC4559.367795E-1012.4820674
ACCGGTA3153.5981138E-612.39659310
GCGTACT2601.0305175E-412.29009349
GACGCGA7050.012.09194669
Produced by FastQC (version 0.11.5)