FastQCFastQC Report
Thu 26 May 2016
SRR936068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622339
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98140.2123167513243836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.1591618442524445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.10994433770435272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA19400.046.330826100-101
GGTATCA118000.036.5660441
CGGTATC17850.035.848007100-101
CCTGTCG12950.034.46853696-97
CGAGTAC13950.031.362303100-101
CGTACAT25000.030.833622100-101
GTATCAA233400.030.5476571
ACGTATC8750.029.592117100-101
TACGCAG13350.029.204842100-101
CCGTATC7850.028.814344100-101
AGGTATC30400.028.783165100-101
TCGTATC9500.027.882467100-101
GCGTACA8350.026.732502100-101
TCGAGTA5600.026.57338100-101
GGGTATC20750.026.104664100-101
GCGTATC5600.026.041912100-101
CCGGTAT8650.025.805363100-101
CCGTACA11600.025.657057100-101
TCGTACA13700.024.982857100-101
TCCGGTA4650.024.95994298-99
Produced by FastQC (version 0.11.5)