FastQCFastQC Report
Thu 26 May 2016
SRR936067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112423
Sequences flagged as poor quality0
Sequence length77
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310362.789945910863044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224452.0176677396997365No Hit
TTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAG11970.10760295319316483No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG11620.10445666801207813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG200.006573740453.24794442
CCGTACG556.0810216E-638.7240379
TCACCGT1107.8034645E-1032.2714840
CTACCCG450.004022876331.5500832
CGTACGT703.170377E-530.42602710
ACGTATA703.172324E-530.4232923
GTCACGC604.5844138E-429.5861849
ATCGCGT604.5916613E-429.5782012
CGTGAAA500.00673058628.39762513
CGTATAG755.0775107E-528.3963494
CAGCTTA1455.0567905E-1026.92999526
TCAGCGC807.877425E-526.6227766
GTTCCGT851.1894695E-425.056736
GAGCGTA2300.024.6935888
AGCTTAG1451.4879333E-824.48181327
AGCGTAG2500.024.1379839
AACGCGA1652.2555469E-923.66894553
TACGTGT901.7524822E-423.66575212
TAACGCG1703.1868694E-922.972852
GAGATAT1553.0027877E-822.91470369
Produced by FastQC (version 0.11.5)