##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936067_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1112423 Sequences flagged as poor quality 0 Sequence length 77 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.55584970824947 39.0 36.0 41.0 30.0 41.0 2 36.36823043033091 39.0 36.0 41.0 30.0 41.0 3 36.26293415364479 39.0 35.0 41.0 30.0 41.0 4 36.13627370164048 39.0 35.0 41.0 29.0 41.0 5 36.00254040054907 39.0 35.0 41.0 29.0 41.0 6 35.82879893709497 39.0 35.0 40.0 29.0 41.0 7 35.56080735475624 38.0 35.0 40.0 28.0 41.0 8 35.2377171273877 38.0 35.0 40.0 27.0 41.0 9 35.053075134189065 38.0 34.0 40.0 27.0 41.0 10-11 35.1373070315878 38.0 35.0 40.0 27.0 41.0 12-13 35.09855558542029 38.0 35.0 40.0 26.5 41.0 14-15 34.89891749810998 38.0 35.0 41.0 25.0 41.0 16-17 34.58473620196634 38.0 34.0 41.0 24.0 41.0 18-19 34.219590928990144 38.0 34.0 40.0 22.0 41.0 20-21 33.71857467887665 38.0 33.0 40.0 16.0 41.0 22-23 33.085524571138855 38.0 33.0 40.0 4.5 41.0 24-25 32.60302061356157 38.0 33.0 40.0 2.0 41.0 26-27 31.60106002842444 37.0 31.0 39.5 2.0 40.5 28-29 31.59086381709116 37.0 31.0 39.5 2.0 40.5 30-31 31.90152981374891 37.0 32.0 40.0 2.0 41.0 32-33 31.726223298151872 37.0 31.5 40.0 2.0 41.0 34-35 31.449561003323375 37.0 31.0 40.0 2.0 41.0 36-37 31.16729877034186 36.0 31.0 40.0 2.0 41.0 38-39 30.867950410949792 36.0 30.5 40.0 2.0 41.0 40-41 30.4713800415849 35.5 29.5 39.0 2.0 41.0 42-43 30.10331996012308 35.0 29.0 39.0 2.0 41.0 44-45 29.713683553827995 35.0 29.0 39.0 2.0 40.5 46-47 29.30869147797196 35.0 28.5 38.0 2.0 40.0 48-49 28.820978620542725 35.0 28.0 37.5 2.0 40.0 50-51 28.35861852910269 35.0 26.0 37.0 2.0 39.0 52-53 27.874900105445498 34.0 26.0 36.0 2.0 39.0 54-55 27.36232575198463 34.0 25.5 36.0 2.0 38.5 56-57 26.85038020609067 34.0 24.0 35.5 2.0 37.0 58-59 26.351566805073247 34.0 21.0 35.0 2.0 37.0 60-61 25.912427646677568 33.0 20.0 35.0 2.0 36.5 62-63 25.501378072909315 33.0 17.5 35.0 2.0 36.0 64-65 25.045470113437062 33.0 8.5 35.0 2.0 36.0 66-67 24.579179412867227 33.0 2.0 35.0 2.0 35.0 68-69 24.119639741357382 32.5 2.0 35.0 2.0 35.0 70-71 23.623520009924285 32.0 2.0 35.0 2.0 35.0 72-73 23.081707677744888 32.0 2.0 35.0 2.0 35.0 74-75 22.470862702407267 31.5 2.0 35.0 2.0 35.0 76-77 20.949654942409495 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.075018955756292 1101 2 1.1736686555341151 1101 3 1.0957473899825985 1101 4 1.166357474163 1101 5 0.9337252156955884 1101 6 0.9262464193912265 1101 7 1.1657557475879656 1101 8 1.2237002776859498 1101 9 1.2343093789859623 1101 10-11 1.1946647718553223 1101 12-13 1.2891069888529145 1101 14-15 1.2508534542272542 1101 16-17 1.3915902122335453 1101 18-19 1.52410539331877 1101 20-21 1.6301890700032686 1101 22-23 1.6218591029934188 1101 24-25 1.4455378320728798 1101 26-27 1.1211627861760647 1101 28-29 1.0332065075592567 1101 30-31 1.0788323258099588 1101 32-33 1.2570268433538558 1101 34-35 1.3041093206342644 1101 36-37 1.4142168765028522 1101 38-39 1.2939115091934497 1101 40-41 1.239686070495999 1101 42-43 1.0677011098574134 1101 44-45 1.0497677448627307 1101 46-47 1.0387724149943125 1101 48-49 1.0703603095573158 1101 50-51 1.025710665464068 1101 52-53 1.0755297363601777 1101 54-55 1.0847398172476233 1101 56-57 0.7807794318185977 1101 58-59 0.7376327834394409 1101 60-61 0.6101731156327652 1101 62-63 0.9290270411341055 1101 64-65 1.0193183878743532 1101 66-67 1.0372638939223116 1101 68-69 0.9577940339760502 1101 70-71 1.0396613340559036 1101 72-73 0.9022525748651375 1101 74-75 1.0624098266894144 1101 76-77 1.2010632637615757 1102 1 0.9754640744849894 1102 2 1.184063111824095 1102 3 1.0863279122795362 1102 4 1.0675110469583657 1102 5 1.0446039720967306 1102 6 1.0641603296931947 1102 7 0.9678943221146028 1102 8 0.8313055662613849 1102 9 0.851905920089969 1102 10-11 1.008857316458318 1102 12-13 1.171415911273776 1102 14-15 1.1294276163835022 1102 16-17 1.223111406627872 1102 18-19 1.1535543026184314 1102 20-21 0.7578531762121514 1102 22-23 0.9374867796285571 1102 24-25 1.1218745550513987 1102 26-27 1.021552094214627 1102 28-29 1.0025208789258642 1102 30-31 1.0532402768970108 1102 32-33 1.1365383973057135 1102 34-35 0.9426428434961558 1102 36-37 1.0600319859373748 1102 38-39 1.0727986668119485 1102 40-41 1.3612326416694351 1102 42-43 1.2772725293168925 1102 44-45 1.002243233718815 1102 46-47 0.685096654602134 1102 48-49 0.6947668938519591 1102 50-51 0.7914287781971048 1102 52-53 1.0436698052465125 1102 54-55 1.14349025006522 1102 56-57 1.063518666050367 1102 58-59 1.081791112672395 1102 60-61 0.9491729425057223 1102 62-63 1.0321184977703375 1102 64-65 1.0351800138106064 1102 66-67 0.9948990241320672 1102 68-69 0.7988406324853301 1102 70-71 1.117149351842425 1102 72-73 0.9417175667555036 1102 74-75 1.0084727811478906 1102 76-77 1.2449646360211588 1103 1 0.2765631446716128 1103 2 0.16103978955473508 1103 3 0.204816470958896 1103 4 0.3372800224051389 1103 5 0.5616351637086296 1103 6 0.6140559180254712 1103 7 0.7068915304763266 1103 8 0.7091373205972218 1103 9 0.4427572598565277 1103 10-11 0.578803242205133 1103 12-13 0.452300882588041 1103 14-15 0.5634757116948705 1103 16-17 0.5611128574005235 1103 18-19 0.7505923084654853 1103 20-21 0.8902218922783547 1103 22-23 0.8038167644613878 1103 24-25 0.8155021461093597 1103 26-27 0.419212173787308 1103 28-29 0.43906935891179444 1103 30-31 0.6952335505874636 1103 32-33 0.6201105952262722 1103 34-35 0.7407645325472139 1103 36-37 0.6566578404644403 1103 38-39 0.6172461642291331 1103 40-41 0.6903887755801463 1103 42-43 0.7072123002706654 1103 44-45 0.529328719397931 1103 46-47 0.2323665642585624 1103 48-49 0.14179061178658614 1103 50-51 0.3047220321043014 1103 52-53 0.5976550337432052 1103 54-55 0.6804453860201995 1103 56-57 0.4906349256947067 1103 58-59 0.3066520802937873 1103 60-61 0.3222259396701226 1103 62-63 0.3753119561045857 1103 64-65 0.4682147664099041 1103 66-67 0.3903049107519898 1103 68-69 0.4395765698822949 1103 70-71 0.3815416759362442 1103 72-73 0.4453543900030361 1103 74-75 0.3293375295707257 1103 76-77 0.26451819371135343 1104 1 0.25976389148547696 1104 2 0.32918046655773736 1104 3 -0.00407426692567725 1104 4 0.08887917818076829 1104 5 0.3649958261000563 1104 6 0.11082310692066955 1104 7 0.1581172793195762 1104 8 0.3618404620859721 1104 9 0.19909503077948898 1104 10-11 0.11100756073748386 1104 12-13 0.41236561998555743 1104 14-15 0.19386778897386847 1104 16-17 0.45770754852804174 1104 18-19 0.43101787060526675 1104 20-21 0.20445803157537767 1104 22-23 0.05035361810210759 1104 24-25 -0.009527279980417802 1104 26-27 0.04105140586783307 1104 28-29 -0.07341237661868405 1104 30-31 0.10568894469413337 1104 32-33 0.16969761527660054 1104 34-35 0.04798508616358177 1104 36-37 -0.09859522390766884 1104 38-39 -0.041377709461912104 1104 40-41 0.06983110044417629 1104 42-43 0.12638187572322224 1104 44-45 0.08459091600479951 1104 46-47 -0.005434799488494235 1104 48-49 0.06468613902094233 1104 50-51 0.26660002544791794 1104 52-53 0.25969842200523985 1104 54-55 0.35079910791321467 1104 56-57 0.3288857983304716 1104 58-59 0.21826320944387945 1104 60-61 0.3717612103901473 1104 62-63 0.40490445549577103 1104 64-65 0.24336189685880427 1104 66-67 0.1208958678101908 1104 68-69 0.17931802616933012 1104 70-71 0.22799836884962943 1104 72-73 0.166255031288852 1104 74-75 0.07203782991978258 1104 76-77 0.0713186645287891 1105 1 -0.2423331201269434 1105 2 -0.12591096860040807 1105 3 -0.11229427985432494 1105 4 -0.1649215336738692 1105 5 -0.0651271518511436 1105 6 -0.21557178548335543 1105 7 -0.35726664709614653 1105 8 0.00808803796391544 1105 9 -0.19500977696524302 1105 10-11 -0.035827061566770624 1105 12-13 0.0561360157113171 1105 14-15 0.009603359119594757 1105 16-17 -0.005286135124997315 1105 18-19 0.2064648241945264 1105 20-21 0.11914548533209057 1105 22-23 0.021417486416787312 1105 24-25 0.06610581502959434 1105 26-27 -0.1847779554100768 1105 28-29 -0.07571237194567715 1105 30-31 0.08901994152428117 1105 32-33 0.1327132036470431 1105 34-35 0.305757853440074 1105 36-37 0.3123835178074792 1105 38-39 0.055885563823288464 1105 40-41 0.06377176883985669 1105 42-43 -0.13341196681204082 1105 44-45 -0.1403160132848278 1105 46-47 -0.3816478340609528 1105 48-49 -0.11831268493912006 1105 50-51 0.02485469650720873 1105 52-53 -0.2050568876982588 1105 54-55 -0.24706386785724987 1105 56-57 -0.152848101215465 1105 58-59 0.11475368429921318 1105 60-61 -0.11750353099789024 1105 62-63 -0.12538246709587852 1105 64-65 0.08255547225386906 1105 66-67 0.06700247466237741 1105 68-69 -0.28472416328992267 1105 70-71 -0.4082703913238923 1105 72-73 -0.3305059911649444 1105 74-75 -0.26002892397633914 1105 76-77 -0.0610724738934465 1106 1 -0.03675343057187064 1106 2 0.018560067182683326 1106 3 -0.20550196667149834 1106 4 0.04134165418173552 1106 5 -0.09177870344110772 1106 6 -0.13299835579629615 1106 7 -0.02809386556075566 1106 8 -0.11001168053242338 1106 9 -0.20669369219973532 1106 10-11 -0.26122331970650947 1106 12-13 -0.1180281390990956 1106 14-15 -0.06138868007806053 1106 16-17 -0.06524602353901798 1106 18-19 0.04413348960550678 1106 20-21 -0.20092261062716688 1106 22-23 -0.15552264791992343 1106 24-25 0.053433268324688754 1106 26-27 -0.2803583577562314 1106 28-29 -0.05899497994084868 1106 30-31 0.04358488020959683 1106 32-33 0.0593967820145167 1106 34-35 0.30471258925333444 1106 36-37 0.12233242142738376 1106 38-39 0.1280200216089682 1106 40-41 0.045867042644395895 1106 42-43 0.15728913645185472 1106 44-45 -0.10053104185017148 1106 46-47 0.09623269629467046 1106 48-49 -0.09010861578080664 1106 50-51 -0.2967195367785713 1106 52-53 0.04635136290736952 1106 54-55 0.01233545430772054 1106 56-57 -0.18485535020315425 1106 58-59 0.04769176032960942 1106 60-61 -0.08498902862859481 1106 62-63 -0.24353784188240724 1106 64-65 -0.0697133027121275 1106 66-67 0.051901067925232525 1106 68-69 -0.08937693150686243 1106 70-71 -0.12240699138853373 1106 72-73 -0.059051223617831994 1106 74-75 -0.12374863889896304 1106 76-77 -0.1531714288245496 1107 1 0.05232424045593831 1107 2 0.11298790436040917 1107 3 0.2395146946653952 1107 4 0.12193710579897044 1107 5 0.07013426830182112 1107 6 0.026180210170089424 1107 7 -0.06129974777739022 1107 8 0.31217686369774356 1107 9 0.29459889385294247 1107 10-11 0.30660199347564543 1107 12-13 0.2936347967257902 1107 14-15 0.26809794682385757 1107 16-17 0.19052334092019407 1107 18-19 0.33658282199338885 1107 20-21 0.2621666190582843 1107 22-23 0.20264789557798935 1107 24-25 0.02844682448891689 1107 26-27 0.021119148280313027 1107 28-29 0.13155278178581398 1107 30-31 0.29353227765779266 1107 32-33 0.32634940264121326 1107 34-35 0.31600290435884304 1107 36-37 0.414179257627211 1107 38-39 0.46194148391356293 1107 40-41 0.6382204391007917 1107 42-43 0.7322836908132366 1107 44-45 0.7926969009961091 1107 46-47 0.857329053973487 1107 48-49 0.9370013953885419 1107 50-51 0.817928072470206 1107 52-53 0.890029555789571 1107 54-55 0.7678007899212709 1107 56-57 0.7167009437964431 1107 58-59 0.930518301677072 1107 60-61 0.819401778206494 1107 62-63 0.9495774805225743 1107 64-65 0.9804866796764458 1107 66-67 1.047717427087111 1107 68-69 0.7940947442204198 1107 70-71 0.8115886242930515 1107 72-73 1.0643854146388207 1107 74-75 1.1215692705319569 1107 76-77 0.8440932384816904 1108 1 0.07771346304638627 1108 2 0.21227159671058615 1108 3 -0.06371559392891868 1108 4 -0.03360953187018367 1108 5 -0.10733656157943017 1108 6 -0.03735023722013864 1108 7 -0.2112160347146954 1108 8 0.0398453254486455 1108 9 -0.16622166676567218 1108 10-11 -0.19213842698831485 1108 12-13 -0.1474385428212983 1108 14-15 -0.08113655331422365 1108 16-17 -0.04270858741451633 1108 18-19 -0.17841864514276295 1108 20-21 -0.16124585332503472 1108 22-23 -0.2389259963027115 1108 24-25 -0.14022291453344593 1108 26-27 -0.14634365550316986 1108 28-29 0.22344814908766253 1108 30-31 0.1758856503448989 1108 32-33 0.19555594089031203 1108 34-35 0.24508154285649297 1108 36-37 0.20499584834800544 1108 38-39 0.18160240285860496 1108 40-41 0.2643294214261154 1108 42-43 0.29695418929431483 1108 44-45 0.12520216542582574 1108 46-47 0.1119915263568032 1108 48-49 0.11971378492182083 1108 50-51 0.12636583137658874 1108 52-53 -0.060252220653399746 1108 54-55 0.15156045575944077 1108 56-57 0.0936604335782718 1108 58-59 -0.10302992395120825 1108 60-61 0.10430883081626874 1108 62-63 0.16908564470373832 1108 64-65 0.06182168727103132 1108 66-67 -0.1535512141722073 1108 68-69 -0.20670052987513543 1108 70-71 -0.3431825887696398 1108 72-73 -0.18425446384778255 1108 74-75 -0.21476306169670778 1108 76-77 -0.1549652053183621 1109 1 0.39111908905825743 1109 2 0.35325581149741936 1109 3 0.131223356421863 1109 4 0.44866751885836464 1109 5 0.2496234357222491 1109 6 0.42346668129038534 1109 7 0.328380228102354 1109 8 0.5722319957130821 1109 9 0.3861697255710794 1109 10-11 0.5459223190991125 1109 12-13 0.6837347235035836 1109 14-15 0.7819520192707756 1109 16-17 1.086498273081979 1109 18-19 0.7200475245820073 1109 20-21 0.511076900317704 1109 22-23 0.6851843990022033 1109 24-25 0.558928798904212 1109 26-27 0.8014546782798888 1109 28-29 0.8324723665728158 1109 30-31 0.6876292755472697 1109 32-33 0.8672117018185332 1109 34-35 1.0070654878106176 1109 36-37 0.9267325590873448 1109 38-39 0.8970343038300008 1109 40-41 1.0624591434984296 1109 42-43 1.16908215745406 1109 44-45 0.9144417431376439 1109 46-47 0.6130059286773673 1109 48-49 0.8730116464976447 1109 50-51 0.9920009501029803 1109 52-53 1.0423963559790899 1109 54-55 0.9841056527373091 1109 56-57 0.6657555158772475 1109 58-59 0.6997240560811697 1109 60-61 0.673110267675419 1109 62-63 0.4743330475639631 1109 64-65 0.5088796675732468 1109 66-67 0.5392408797295722 1109 68-69 0.630667083454604 1109 70-71 0.5668627768536787 1109 72-73 0.6214255145655976 1109 74-75 0.5251097796416637 1109 76-77 0.5917242846978255 1110 1 -0.45070909531838055 1110 2 -0.3326138401331278 1110 3 -0.43463929919958844 1110 4 -0.38672433715104404 1110 5 -0.42635640305154254 1110 6 -0.5125802555706329 1110 7 -0.4475439605990914 1110 8 -0.3995406975914193 1110 9 -0.382410243582882 1110 10-11 -0.6093437677468003 1110 12-13 -0.5046288463690587 1110 14-15 -0.4365993298459827 1110 16-17 -0.2575519391833083 1110 18-19 -0.16903552122595045 1110 20-21 -0.4520175617050981 1110 22-23 -0.6971141079858612 1110 24-25 -0.5232496629277819 1110 26-27 -0.520861795731534 1110 28-29 -0.36528573051270286 1110 30-31 -0.20590100604209383 1110 32-33 -0.05014794074899953 1110 34-35 -0.018295840159105126 1110 36-37 0.008608920526615549 1110 38-39 -0.15541191435008272 1110 40-41 -0.0630309746387212 1110 42-43 -0.10430836295362056 1110 44-45 -0.18723491404200843 1110 46-47 -0.2667016699425133 1110 48-49 -0.21856690755447516 1110 50-51 -0.2069138734862932 1110 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-1.2669224989832983 2314 22-23 -1.0980165075071895 2314 24-25 -0.9767950433849926 2314 26-27 -1.061653529452748 2314 28-29 -1.098308678643427 2314 30-31 -1.169746352456226 2314 32-33 -1.1917864960844788 2314 34-35 -1.1276863142863611 2314 36-37 -1.2725295368060543 2314 38-39 -1.1163449010629662 2314 40-41 -0.9766982396383099 2314 42-43 -0.9621275684087642 2314 44-45 -0.8858053625853763 2314 46-47 -1.0323318279849332 2314 48-49 -0.9568355848260808 2314 50-51 -0.9812147008363006 2314 52-53 -0.8507809321379902 2314 54-55 -0.8985095722517649 2314 56-57 -0.6863203087186456 2314 58-59 -0.7033012824946248 2314 60-61 -0.6503961761865291 2314 62-63 -0.7195917012346378 2314 64-65 -0.6866683337373694 2314 66-67 -0.6026933710349525 2314 68-69 -0.41724412231210906 2314 70-71 -0.263919633587566 2314 72-73 -0.17199956188699872 2314 74-75 -0.3032609608563739 2314 76-77 -0.23151457506626372 2315 1 -1.6854683128285188 2315 2 -1.2919865939074313 2315 3 -1.4910122246065072 2315 4 -1.3601522533891668 2315 5 -1.2681588810135267 2315 6 -1.3716531039364455 2315 7 -1.7831515335977954 2315 8 -1.8001118599571626 2315 9 -1.754759455298803 2315 10-11 -1.7038717199890598 2315 12-13 -1.6933769735733435 2315 14-15 -2.007804926359647 2315 16-17 -1.745967383021373 2315 18-19 -1.8838647080616013 2315 20-21 -1.7725230778224343 2315 22-23 -1.9435111135774719 2315 24-25 -2.0954653754418686 2315 26-27 -2.1055592642150103 2315 28-29 -1.916990700502808 2315 30-31 -1.9811729404965028 2315 32-33 -1.921451225900455 2315 34-35 -1.8299603409856537 2315 36-37 -2.258064929760703 2315 38-39 -2.168994441891954 2315 40-41 -2.0812937170697907 2315 42-43 -2.107562285016929 2315 44-45 -1.9931371402331486 2315 46-47 -1.9129700801212302 2315 48-49 -1.86124651850335 2315 50-51 -2.059866018958484 2315 52-53 -2.025750405686903 2315 54-55 -2.0329378688868758 2315 56-57 -2.2498510612095153 2315 58-59 -2.2132426007941 2315 60-61 -2.4402334718642926 2315 62-63 -2.5541028791235156 2315 64-65 -2.5076996734884602 2315 66-67 -2.3901251158847927 2315 68-69 -2.4428032383635916 2315 70-71 -2.565963908576041 2315 72-73 -3.531704203237208 2315 74-75 -4.059641598554489 2315 76-77 -3.625611612615458 2316 1 -3.1586217038919244 2316 2 -2.845957708865548 2316 3 -2.4739607845744445 2316 4 -2.349627800008669 2316 5 -3.0120809372894115 2316 6 -2.6142119327799733 2316 7 -3.1820504484550014 2316 8 -3.028982772541184 2316 9 -2.5923418944537246 2316 10-11 -2.4702324810457057 2316 12-13 -2.621904281572718 2316 14-15 -2.740055930258954 2316 16-17 -4.036388592476996 2316 18-19 -3.8940790670571204 2316 20-21 -3.0140268980477103 2316 22-23 -2.8800367935813185 2316 24-25 -3.139482835756347 2316 26-27 -2.869131676568017 2316 28-29 -2.8686387958918402 2316 30-31 -2.7802617881645126 2316 32-33 -2.993028324679898 2316 34-35 -3.0416785327102573 2316 36-37 -2.8475312235638803 2316 38-39 -3.078876387209057 2316 40-41 -2.8328477838388615 2316 42-43 -3.2944366649063035 2316 44-45 -3.1279769320947928 2316 46-47 -2.526175161918001 2316 48-49 -2.633539397648434 2316 50-51 -3.281545680205376 2316 52-53 -4.195913136468086 2316 54-55 -3.493968820182676 2316 56-57 -3.310783357477195 2316 58-59 -3.2727657181429386 2316 60-61 -3.1373631607133383 2316 62-63 -3.2049169081435984 2316 64-65 -3.0835605761507097 2316 66-67 -3.0877374516394873 2316 68-69 -3.1375309461096386 2316 70-71 -3.283025914324039 2316 72-73 -3.3371968750692 2316 74-75 -3.13603766410694 2316 76-77 -3.046934540281569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31131.0 3 3690.0 4 6365.0 5 7752.0 6 8377.0 7 8608.0 8 11132.0 9 12653.0 10 18119.0 11 20426.0 12 13548.0 13 8466.0 14 7996.0 15 7733.0 16 7793.0 17 8102.0 18 8717.0 19 9055.0 20 9255.0 21 9622.0 22 10839.0 23 11911.0 24 13346.0 25 14960.0 26 17004.0 27 19298.0 28 22122.0 29 24887.0 30 29203.0 31 34187.0 32 41103.0 33 50903.0 34 70620.0 35 90606.0 36 108525.0 37 147835.0 38 144928.0 39 41419.0 40 187.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.874769060644336 28.583437096839447 35.20850845766224 17.333285384853976 2 18.13457620596586 29.051608344077206 35.31497075329374 17.498844696663195 3 18.059005722557124 29.425181948546435 34.50238926874441 18.013423060152032 4 18.882793169917715 27.760965101559353 35.55269650152483 17.803545226998104 5 17.955755904067665 28.86196331152835 35.436093659533 17.746187124870985 6 18.1815484725917 29.32395584320544 34.650713066243284 17.843782617959576 7 18.82288736224957 28.78362450067296 34.936484431965205 17.457003705112264 8 17.501755471181053 29.91511701193181 34.591997928499055 17.991129588388084 9 17.718605470801055 29.927029612526884 34.588557063634205 17.765807853037856 10-11 18.11087339332573 29.415485483005927 34.68056229272435 17.793078830943994 12-13 18.278429116018124 29.287698698122703 33.6831978709631 18.750674314896067 14-15 17.882912445047783 29.868249530265302 34.09698560678935 18.151852417897565 16-17 17.860766133726614 29.692008326504705 34.29176838436411 18.155457155404562 18-19 17.993736016287976 29.571406672082208 33.358747706749895 19.07610960487992 20-21 17.86936003564224 29.840801285062852 33.11388118743332 19.175957491861585 22-23 18.266189288482323 29.907407573912998 32.49330597579901 19.333097161805668 24-25 18.181638357883795 30.573620368297398 31.43448249818603 19.81025877563278 26-27 18.598702060652926 30.625164377926488 30.744572999534686 20.0315605618859 28-29 18.556137521329255 30.740064477732165 30.705721904268728 19.99807609666985 30-31 18.812639123133188 30.583355209453362 30.51894052452343 20.08506514289002 32-33 18.910543267426206 30.715073979758113 29.96927530629171 20.405107446523974 34-35 18.393397375672603 30.11593251729548 30.8913183221926 20.599351784839318 36-37 19.05172254910026 30.955941815559875 30.16190464631317 19.8304309890267 38-39 19.242623127685974 30.751667775520115 30.776077535827955 19.229631560965963 40-41 19.19329092511853 30.12713923461129 30.944960574385423 19.73460926588476 42-43 19.053591457014228 30.657154480864367 30.378666810518247 19.91058725160316 44-45 18.967424652792605 30.41147925296747 30.825723128653852 19.79537296558607 46-47 19.153870085402456 30.059632458328313 30.74110773225818 20.045389724011056 48-49 18.57948752070292 30.267263222444214 30.409868346517865 20.743380910335006 50-51 18.19463272835185 30.70729552367656 30.396194304496106 20.701877443475485 52-53 17.801844708345342 30.203738068141707 31.354915932360996 20.63950129115196 54-55 17.64188841356086 29.74577318985071 31.393245646177604 21.219092750410823 56-57 17.78723970520866 30.579229106171546 30.43266963472075 21.200861553899045 58-59 18.145204613612016 29.46857688222001 30.547714704820628 21.838503799347347 60-61 17.72480932932024 29.220930729167836 30.667622736172024 22.386637205339905 62-63 17.800864928925932 29.529918007543287 30.721162254426144 21.94805480910464 64-65 17.941325099269147 28.698540096104047 31.07452559417621 22.285609210450595 66-67 17.987883420067664 28.7108543425238 30.960581775674672 22.34068046173386 68-69 18.259817367202718 28.630442876439577 30.8056599260044 22.304079830353306 70-71 17.918361326322643 27.812895183239107 31.43243637043926 22.83630711999899 72-73 18.207235155138008 27.77544351493798 31.104752119280732 22.91256921064328 74-75 18.134027426642458 27.602579997372857 31.186694425600596 23.076698150384082 76-77 18.17166739021389 27.13324590783433 31.769916480906225 22.92517022104555 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 39529.0 1 22950.0 2 5655.0 3 4737.5 4 4536.0 5 4444.5 6 4222.5 7 3999.0 8 3906.5 9 3907.0 10 3863.5 11 3808.0 12 3944.5 13 4093.0 14 4224.0 15 4552.5 16 4963.5 17 5177.0 18 5464.5 19 6041.5 20 6767.5 21 7703.5 22 8203.0 23 8561.5 24 9514.5 25 10999.0 26 11889.0 27 12918.0 28 15863.5 29 20422.0 30 23064.0 31 27179.0 32 33781.0 33 40236.0 34 46949.5 35 49695.0 36 54496.0 37 61215.5 38 60725.0 39 58316.0 40 57715.5 41 55888.5 42 53376.0 43 52090.0 44 48171.0 45 42844.5 46 40085.5 47 38154.0 48 37574.0 49 35242.0 50 31452.0 51 27760.5 52 25527.0 53 23809.0 54 20560.0 55 18122.5 56 16171.0 57 15126.0 58 15203.5 59 14243.5 60 12383.5 61 11561.0 62 10380.5 63 7319.5 64 4582.0 65 3725.0 66 3295.5 67 2615.5 68 2063.5 69 1692.5 70 1623.0 71 1398.0 72 999.0 73 743.0 74 661.0 75 606.5 76 445.0 77 295.5 78 253.0 79 208.5 80 163.5 81 149.0 82 101.5 83 68.0 84 63.0 85 43.0 86 23.0 87 18.0 88 22.0 89 25.0 90 18.5 91 13.0 92 12.0 93 8.0 94 8.0 95 12.0 96 13.0 97 12.0 98 11.5 99 28.5 100 45.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010607475753377987 2 0.014472911832998777 3 0.014203230246048491 4 0.015012275006899355 5 0.012495246862030001 6 0.010247900304110937 7 0.015821319767750218 8 0.016720258390917845 9 0.017169727702501657 10-11 0.02036095981474673 12-13 0.016450576803967554 14-15 0.008360129195458922 16-17 0.006427411155648525 18-19 0.017214674633660037 20-21 0.023327457271199895 22-23 0.021304845369072734 24-25 0.020136225158954822 26-27 0.024765759068268098 28-29 0.009079280093993022 30-31 0.008899492369359496 32-33 0.011281679720753706 34-35 0.012405352999713239 36-37 0.011910936756971044 38-39 0.014652699557632303 40-41 0.01245029993087162 42-43 0.014877434213424209 44-45 0.013978495590256584 46-47 0.02490059986174324 48-49 0.02346229806467504 50-51 0.03645196116944724 52-53 0.03878920158968306 54-55 0.029260452184106223 56-57 0.02215883706108198 58-59 0.0173045684959768 60-61 0.020720535264013778 62-63 0.027732256524721263 64-65 0.026518689383444965 66-67 0.027912044249354787 68-69 0.041800645977294604 70-71 0.053666635803107265 72-73 0.08638800168640887 74-75 0.08530927533860772 76-77 0.08993880924792098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1112423.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.487378978086554 #Duplication Level Percentage of deduplicated Percentage of total 1 81.58532127431316 35.479317853050034 2 8.651201186772301 7.524361292496784 3 3.2569153311228263 4.249041339122357 4 1.7435671815713114 3.032926671949835 5 1.0184241164167762 2.2144297755519644 6 0.6712105278063031 1.7513511958076524 7 0.48202213217119827 1.467331539628796 8 0.3396991192001564 1.181809946814352 9 0.24267923766690788 0.9498135580680565 >10 1.6049060133763053 13.717976738395976 >50 0.22026545138786116 6.7321100559341485 >100 0.17467152761736093 14.198315542154658 >500 0.00808088914823929 2.3695396582559805 >1k 6.216068575568685E-4 0.31267074625045643 >5k 0.0 0.0 >10k+ 4.14404571704579E-4 4.819004086518922 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31036 2.789945910863044 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22445 2.0176677396997365 No Hit TTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAG 1197 0.10760295319316483 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1162 0.10445666801207813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0010787263478011513 0.0 2 0.0 0.0 0.0 0.007371296709974533 0.0 3 0.0 0.0 0.0 0.010877157340328274 0.0 4 0.0 0.0 0.0 0.018158560187986044 0.0 5 0.0 0.0 0.0 0.031642639535500436 0.0 6 0.0 0.0 0.0 0.05177886469445526 0.0 7 0.0 0.0 0.0 0.08746672803421 0.0 8 0.0 0.0 0.0 0.12144660798994628 0.0 9 0.0 0.0 0.0 0.16324725396724088 0.0 10 0.0 0.0 0.0 0.21295855982841058 0.0 11 0.0 0.0 0.0 0.2739964923414924 0.0 12 0.0 0.0 0.0 0.3496871244122065 0.0 13 0.0 0.0 0.0 0.4447049368810246 0.0 14 0.0 0.0 0.0 0.5586004604363628 0.0 15 0.0 0.0 0.0 0.6875082589986004 0.0 16 0.0 0.0 0.0 0.8172251023216888 0.0 17 0.0 0.0 0.0 0.9313903074639773 0.0 18 0.0 0.0 0.0 1.039802305417993 0.0 19 0.0 0.0 0.0 1.1502818622052942 0.0 20 0.0 0.0 0.0 1.274155604477793 0.0 21 0.0 0.0 0.0 1.4135809849310919 0.0 22 0.0 0.0 0.0 1.5568718014640115 0.0 23 0.0 0.0 0.0 1.7220068265399044 0.0 24 0.0 0.0 0.0 1.8802200242174065 0.0 25 0.0 0.0 0.0 2.0498497424091373 0.0 26 0.0 0.0 0.0 2.222805533506589 0.0 27 0.0 0.0 0.0 2.3990873975097604 0.0 28 0.0 0.0 0.0 2.5610761374045663 0.0 29 0.0 0.0 0.0 2.721087212328404 0.0 30 0.0 0.0 0.0 2.8923350200418367 0.0 31 0.0 0.0 0.0 3.0752690298564485 0.0 32 0.0 0.0 0.0 3.2716871190185746 0.0 33 0.0 0.0 0.0 3.473498839919707 0.0 34 0.0 0.0 0.0 3.688165383132136 0.0 35 0.0 0.0 0.0 3.909573966018322 0.0 36 0.0 0.0 0.0 4.151927818824314 0.0 37 0.0 0.0 0.0 4.386820481058015 0.0 38 0.0 0.0 0.0 4.602745538342878 0.0 39 0.0 0.0 0.0 4.821277517634928 0.0 40 0.0 0.0 0.0 5.050416972680356 0.0 41 0.0 0.0 0.0 5.30715384345703 0.0 42 0.0 0.0 0.0 5.568475301211859 0.0 43 0.0 0.0 0.0 5.845707972596755 0.0 44 0.0 0.0 0.0 6.1388518576117175 0.0 45 0.0 0.0 0.0 6.436580329604835 0.0 46 0.0 0.0 0.0 6.75300672495984 0.0 47 0.0 0.0 0.0 7.078871975858104 0.0 48 0.0 0.0 0.0 7.39386006941604 0.0 49 0.0 0.0 0.0 7.709926889321777 0.0 50 0.0 0.0 0.0 8.013228780778535 0.0 51 0.0 0.0 0.0 8.354645669857598 0.0 52 0.0 8.989386231676259E-5 0.0 8.687073172704988 0.0 53 0.0 8.989386231676259E-5 0.0 9.025613458189914 0.0 54 0.0 8.989386231676259E-5 0.0 9.398493199079846 0.0 55 0.0 8.989386231676259E-5 0.0 9.78602563952741 0.0 56 0.0 8.989386231676259E-5 0.0 10.203582629988773 0.0 57 0.0 8.989386231676259E-5 0.0 10.612509809667726 0.0 58 0.0 8.989386231676259E-5 0.0 11.030696057165304 0.0 59 0.0 8.989386231676259E-5 0.0 11.447174321278865 0.0 60 0.0 8.989386231676259E-5 0.0 11.8538541543999 0.0 61 0.0 8.989386231676259E-5 0.0 12.294963336788253 0.0 62 0.0 8.989386231676259E-5 0.0 12.724116635488478 0.0 63 0.0 8.989386231676259E-5 0.0 13.153989085087238 0.0 64 0.0 8.989386231676259E-5 0.0 13.590603574359754 0.0 65 0.0 8.989386231676259E-5 0.0 14.035038829653828 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTACG 20 0.0065737404 53.247944 42 CCGTACG 55 6.0810216E-6 38.724037 9 TCACCGT 110 7.8034645E-10 32.27148 40 CTACCCG 45 0.0040228763 31.550083 2 CGTACGT 70 3.170377E-5 30.426027 10 ACGTATA 70 3.172324E-5 30.423292 3 GTCACGC 60 4.5844138E-4 29.58618 49 ATCGCGT 60 4.5916613E-4 29.578201 2 CGTGAAA 50 0.006730586 28.397625 13 CGTATAG 75 5.0775107E-5 28.396349 4 CAGCTTA 145 5.0567905E-10 26.929995 26 TCAGCGC 80 7.877425E-5 26.622776 6 GTTCCGT 85 1.1894695E-4 25.05673 6 GAGCGTA 230 0.0 24.693588 8 AGCTTAG 145 1.4879333E-8 24.481813 27 AGCGTAG 250 0.0 24.137983 9 AACGCGA 165 2.2555469E-9 23.668945 53 TACGTGT 90 1.7524822E-4 23.665752 12 TAACGCG 170 3.1868694E-9 22.9728 52 GAGATAT 155 3.0027877E-8 22.914703 69 >>END_MODULE