FastQCFastQC Report
Thu 26 May 2016
SRR936067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112423
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171371.5405111185223606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129321.162507427480374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99640.8957024441242226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55860.5021471149014358No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33290.2992566676525027No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31800.28586248216730503No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26160.23516234382065093No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.22725168393677586No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT21070.1894063679014188No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.17547281924232058No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.14868444827192534No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT16070.1444594367430375RNA PCR Primer, Index 46 (95% over 21bp)
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT12150.10922104271486656No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA12060.10841199795401571No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11400.10247900304110936No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11340.10193963986720879No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA201.7713355E-459.50685598-99
CCGTCGA150.00423551759.50551696-97
CGTATCA13650.055.147373100-101
TCCGGTA1150.051.74509498-99
CGAATCG1100.051.3911396-97
CGGTATC10100.051.25838100-101
CCTGTCG3700.049.85597696-97
ACGTATC3650.049.724907100-101
TCCCGTA1200.049.58904698-99
CCGTACA3450.049.157837100-101
TTCGGTA850.049.00564698-99
ACCGTAC850.049.00564698-99
CGCACGT255.331247E-447.60441696-97
CGACGCG505.334227E-447.5969289
TGTATCA16400.047.35149100-101
TTTACGC3200.046.4897398-99
TGAGTAC12800.046.48973100-101
CACGTAT2250.046.2831198-99
CCCGGTA2900.046.16911398-99
GGGTATC6800.045.942795100-101
Produced by FastQC (version 0.11.5)