FastQCFastQC Report
Thu 26 May 2016
SRR936066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110540
Sequences flagged as poor quality0
Sequence length77
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312732.8160174329605416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224762.023880274461073No Hit
TTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAG11960.1076953554126821No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG11320.10193239325013057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG458.476908E-539.43428410
ACACGGC552.7563536E-432.26877612
AGCGCTC1258.913048E-1131.2319568
TAACGGC703.1712116E-530.42484712
CGATCCG1102.7686838E-829.05236846
CGCACGA500.006737780828.39140932
GCGGTCT657.3313917E-427.3043512
TCCGCCA1455.0567905E-1026.9253113
GCTCCGC1605.4569682E-1126.61814311
ACGTATA807.8891586E-526.6169453
CGCTCCG1507.512426E-1026.02662810
TCAGCGC1658.0035534E-1125.8115356
CGCGTCA1109.0092E-725.8103712
GGGCCGT1652.2591848E-923.66696720
ATCGCGT750.001690761123.6595082
CGTATAG901.7556189E-423.6595064
GCGTCAA1201.922237E-623.6595063
CGCCAGG1853.3833203E-1023.02420815
ATACGTA3550.022.993041
AATTGGT2500.022.7131271
Produced by FastQC (version 0.11.5)